DREF (ZBED1) (NM_001171136) Human Tagged ORF Clone

SKU RC230438

ZBED1 (Myc-DDK-tagged)-Human zinc finger, BED-type containing 1 (ZBED1), transcript variant 1

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: DREF
• Synonyms: ALTE; DREF; hDREF; TRAMP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC230438 ORF sequence; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGAGAATAAAAGCCTGGAGAGCTCCCAGACAGACCTGAAGCTGGTGGCCCACCCCCGCGCCAAGAGCA; AGGTGTGGAAGTATTTCGGCTTCGACACCAACGCCGAGGGATGCATCCTGCAGTGGAAGAAAATCTACTG; CCGCATCTGCATGGCCCAGATCGCCTACTCCGGAAACACCTCCAACCTGTCCTACCACCTGGAGAAGAAC; CACCCCGAGGAATTCTGCGAGTTCGTCAAGAGCAACACGGAGCAGATGCGTGAAGCCTTCGCCACCGCCT; TCTCCAAGCTGAAGCCCGAGTCGTCCCAGCAGCCCGGGCAGGATGCGCTGGCCGTCAAGGCCGGCCACGG; CTACGACAGCAAGAAGCAGCAGGAGCTGACGGCCGCCGTGCTGGGCCTCATCTGCGAGGGGCTGTACCCA; GCCTCCATCGTGGACGAGCCCACCTTCAAGGTGCTGCTGAAGACGGCCGACCCCCGGTATGAGCTGCCCA; GCCGGAAGTACATCTCTACCAAGGCCATCCCTGAGAAGTACGGGGCCGTCCGGGAGGTGATCCTGAAGGA; GCTGGCCGAGGCCACCTGGTGTGGCATCTCCACCGACATGTGGAGGAGTGAGAATCAGAACCGCGCCTAC; GTCACGCTGGCCGCCCACTTCCTGGGCCTGGGCGCCCCCAACTGCCTGTCCATGGGCTCCCGCTGCCTGA; AGACCTTCGAGGTGCCCGAAGAGAACACGGCGGAGACCATCACGCGAGTGCTCTATGAGGTCTTCATCGA; GTGGGGCATCAGCGCCAAGGTCTTCGGGGCCACCACCAACTATGGCAAGGACATCGTGAAGGCGTGCTCC; CTGCTGGACGTCGCAGTGCACATGCCCTGCCTGGGCCACACCCTCAATGCCGGCATCCAGCAGGCCTTCC; AGCTCCCGAAGCTGGGGGCGCTGCTGTCGCGCTGCCGCAAACTGGTGGAGTACTTCCAGCAGTCTGCCGT; GGCCATGTACATGCTCTATGAGAAGCAGAAGCAGCAGAACGTGGCCCACTGCATGCTGGTGAGCAACCGC; GTCTCCTGGTGGGGGAGCACGCTGGCCATGCTGCAGCGCCTCAAGGAGCAGCAGTTCGTCATCGCCGGGG; TCTTGGTGGAGGACAGCAACAACCACCACCTCATGCTGGAGGCCAGCGAGTGGGCCACCATCGAGGGGCT; GGTGGAGCTCCTGCAGCCCTTCAAGCAGGTGGCCGAGATGCTGTCGGCCTCCAGGTACCCCACCATCAGC; ATGGTGAAGCCGCTGCTGCACATGCTCCTGAACACCACGCTCAACATCAAGGAGACCGACTCCAAGGAGC; TCAGCATGGCCAAGGAGGTCATCGCCAAGGAGCTTTCCAAGACCTACCAGGAGACGCCCGAGATCGACAT; GTTTCTCAACGTGGCCACCTTCCTGGACCCCCGCTACAAGAGGCTGCCCTTCCTCTCCGCCTTCGAGCGG; CAGCAGGTGGAGAATCGCGTGGTGGAAGAGGCCAAGGGCCTGCTGGACAAGGTCAAAGACGGCGGCTACC; GGCCGGCTGAGGACAAGATCTTCCCGGTGCCCGAGGAGCCTCCCGTCAAGAAGCTCATGCGGACATCCAC; GCCGCCGCCCGCCAGCGTCATCAACAACATGCTGGCCGAGATCTTCTGCCAGACAGGCGGCGTGGAGGAC; CAGGAAGAGTGGCATGCCCAGGTGGTGGAGGAGCTGAGCAACTTCAAGTCCCAGAAGGTGCTTGGCCTCA; ACGAAGACCCCCTCAAGTGGTGGTCAGACCGCCTGGCCCTCTTCCCCCTGCTGCCCAAGGTGCTGCAGAA; GTACTGGTGCGTGACGGCCACGCGCGTCGCCCCTGAGCGTCTCTTCGGATCCGCCGCCAACGTGGTCAGC; GCCAAGAGGAACCGGCTGGCTCCCGCGCACGTGGACGAGCAGGTGTTTCTGTATGAGAACGCCCGGAGTG; GGGCAGAGGCGGAACCCGAGGACCAGGACGAGGGGGAGTGGGGCCTGGACCAGGAGCAGGTGTTCTCCTT; GGGGGATGGCGTCAGCGGCGGTTTCTTTGGCATTAGGGACAGCAGCTTCCTG; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC230438 protein sequence; Red=Cloning site Green=Tags(s); ; MENKSLESSQTDLKLVAHPRAKSKVWKYFGFDTNAEGCILQWKKIYCRICMAQIAYSGNTSNLSYHLEKN; HPEEFCEFVKSNTEQMREAFATAFSKLKPESSQQPGQDALAVKAGHGYDSKKQQELTAAVLGLICEGLYP; ASIVDEPTFKVLLKTADPRYELPSRKYISTKAIPEKYGAVREVILKELAEATWCGISTDMWRSENQNRAY; VTLAAHFLGLGAPNCLSMGSRCLKTFEVPEENTAETITRVLYEVFIEWGISAKVFGATTNYGKDIVKACS; LLDVAVHMPCLGHTLNAGIQQAFQLPKLGALLSRCRKLVEYFQQSAVAMYMLYEKQKQQNVAHCMLVSNR; VSWWGSTLAMLQRLKEQQFVIAGVLVEDSNNHHLMLEASEWATIEGLVELLQPFKQVAEMLSASRYPTIS; MVKPLLHMLLNTTLNIKETDSKELSMAKEVIAKELSKTYQETPEIDMFLNVATFLDPRYKRLPFLSAFER; QQVENRVVEEAKGLLDKVKDGGYRPAEDKIFPVPEEPPVKKLMRTSTPPPASVINNMLAEIFCQTGGVED; QEEWHAQVVEELSNFKSQKVLGLNEDPLKWWSDRLALFPLLPKVLQKYWCVTATRVAPERLFGSAANVVS; AKRNRLAPAHVDEQVFLYENARSGAEAEPEDQDEGEWGLDQEQVFSLGDGVSGGFFGIRDSSFL; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Chromatograms: Chromatograms ; Sequencher program is needed, download here
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001171136
• ORF Size: 2082 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001171136.1, NP_001164607.1
• RefSeq Size: 4528 bp
• RefSeq ORF: 2085 bp
• Locus ID: 9189
• UniProt ID: O96006
• Cytogenetics: X;Y
• Protein Families: Druggable Genome
• MW: 78.1 kDa
• Summary: This gene is located in the pseudoautosomal region 1 (PAR1) of X and Y chromosomes. It was earlier identified as a gene with similarity to Ac transposable elements, however, was found not to have transposase activity. Later studies show that this gene product is localized in the nucleus and functions as a transcription factor. It binds to DNA elements found in the promoter regions of several genes related to cell proliferation, such as histone H1, hence may have a role in regulating genes related to cell proliferation. Alternatively spliced transcript variants with different 5' untranslated region have been found for this gene. [provided by RefSeq, Jan 2010]