Kaiso (ZBTB33) (NM_001184742) Human Tagged ORF Clone

SKU RC230416

ZBTB33 (Myc-DDK-tagged)-Human zinc finger and BTB domain containing 33 (ZBTB33), transcript variant 1

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: Kaiso
• Synonyms: ZNF-kaiso; ZNF348
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC230416 representing NM_001184742; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGAGAGTAGAAAACTGATTTCTGCTACAGACATTCAGTACTCTGGCAGTCTGCTGAACTCCTTGAATG; AGCAACGTGGCCATGGACTCTTCTGTGATGTTACCGTTATTGTGGAAGACCGAAAATTCCGGGCTCACAA; GAATATTCTTTCAGCTTCTAGTACCTACTTCCATCAGCTCTTCTCTGTTGCTGGGCAAGTTGTTGAACTG; AGCTTTATAAGAGCAGAGATCTTTGCAGAAATTCTCAATTATATCTATAGTTCTAAAATTGTTCGTGTTA; GATCAGATTTGCTTGATGAGTTAATTAAATCAGGGCAGTTATTAGGAGTGAAATTTATAGCAGAGCTTGG; TGTCCCATTGTCACAGGTTAAAAGCATCTCAGGTACAGCGCAGGATGGTAATACTGAGCCTTTACCTCCT; GATTCTGGTGACAAGAACCTTGTAATACAGAAATCAAAAGATGAAGCCCAAGATAATGGGGCTACTATAA; TGCCTATTATAACAGAGTCTTTTTCATTATCTGCCGAAGATTATGAAATGAAAAAGATCATTGTTACCGA; TTCTGATGATGATGATGATGATGTCATTTTTTGCTCCGAGATTCTGCCCACAAAGGAGACTTTGCCGAGT; AATAACACAGTGGCACAGGTCCAATCTAACCCAGGCCCTGTTGCTATTTCAGATGTTGCACCTAGTGCTA; GCAATAACTCGCCCCCTTTAACAAATATCACACCTACTCAGAAACTTCCTACTCCTGTGAATCAGGCAAC; TTTGAGCCAAACACAAGGAAGTGAAAAATTGTTGGTATCTTCAGCTCCAACACATCTGACTCCCAATATT; ATTTTGTTAAATCAGACACCACTTTCTACACCACCAAATGTCAGTTCTTCACTTCCAAATCATATGCCCT; CTTCAATCAATTTACTTGTGCAGAATCAGCAGACACCAAACAGTGCTATTTTAACAGGAAACAAGGCCAA; TGAAGAGGAGGAGGAGGAAATAATAGATGATGATGATGACACTATTAGCTCCAGTCCTGACTCGGCCGTC; AGTAATACATCTTTGGTCCCACAGGCTGATACCTCCCAAAATACCAGTTTTGATGGATCATTAATACAGA; AGATGCAGATTCCTACACTTCTTCAAGAACCACTTTCCAATTCCTTAAAAATTTCAGATATAATTACTAG; AAATACTAATGATCCAGGCGTAGGATCAAAACATCTAATGGAGGGTCAGAAGATCATTACTTTAGATACA; GCTACTGAAATTGAAGGCTTATCGACTGGTTGCAAGGTTTATGCAAATATCGGTGAAGATACTTATGATA; TAGTGATCCCTGTCAAAGATGACCCTGATGAAGGGGAGGCCAGACTTGAGAATGAAATACCAAAAACGTC; TGGCAGCGAGATGGCAAACAAACGTATGAAAGTAAAACATGATGATCACTATGAGTTAATAGTAGATGGA; AGGGTCTATTATATCTGTATTGTATGCAAAAGGTCATATGTCTGTCTGACAAGCTTGCGGAGACATTTTA; ACATTCATTCTTGGGAGAAGAAGTATCCGTGCCGTTACTGTGAGAAGGTATTTCCTCTTGCAGAATATCG; CACAAAGCATGAAATTCATCACACAGGGGAGCGAAGGTATCAGTGTTTGGCCTGTGGCAAATCTTTCATC; AACTATCAGTTTATGTCTTCACATATAAAGTCAGTTCATAGTCAAGATCCTTCTGGGGACTCAAAGCTTT; ATCGTTTACATCCATGCAGGTCTTTACAAATCAGACAATATGCATATCTTTCCGATAGATCAAGCACTAT; TCCTGCAATGAAGGATGATGGTATTGGGTATAAGGTTGACACTGGAAAAGAACCTCCAGTAGGGACCACT; ACATCTACTCAGAACAAGCCAATGACCTGGGAAGATATTTTTATTCAGCAGGAAAATGATTCAATTTTTA; AACAAAATGTAACAGATGGCAGTACTGAGTTTGAATTTATAATACCAGAGTCTTAC; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC230416 representing NM_001184742; Red=Cloning site Green=Tags(s); ; MESRKLISATDIQYSGSLLNSLNEQRGHGLFCDVTVIVEDRKFRAHKNILSASSTYFHQLFSVAGQVVEL; SFIRAEIFAEILNYIYSSKIVRVRSDLLDELIKSGQLLGVKFIAELGVPLSQVKSISGTAQDGNTEPLPP; DSGDKNLVIQKSKDEAQDNGATIMPIITESFSLSAEDYEMKKIIVTDSDDDDDDVIFCSEILPTKETLPS; NNTVAQVQSNPGPVAISDVAPSASNNSPPLTNITPTQKLPTPVNQATLSQTQGSEKLLVSSAPTHLTPNI; ILLNQTPLSTPPNVSSSLPNHMPSSINLLVQNQQTPNSAILTGNKANEEEEEEIIDDDDDTISSSPDSAV; SNTSLVPQADTSQNTSFDGSLIQKMQIPTLLQEPLSNSLKISDIITRNTNDPGVGSKHLMEGQKIITLDT; ATEIEGLSTGCKVYANIGEDTYDIVIPVKDDPDEGEARLENEIPKTSGSEMANKRMKVKHDDHYELIVDG; RVYYICIVCKRSYVCLTSLRRHFNIHSWEKKYPCRYCEKVFPLAEYRTKHEIHHTGERRYQCLACGKSFI; NYQFMSSHIKSVHSQDPSGDSKLYRLHPCRSLQIRQYAYLSDRSSTIPAMKDDGIGYKVDTGKEPPVGTT; TSTQNKPMTWEDIFIQQENDSIFKQNVTDGSTEFEFIIPESY; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Chromatograms: Chromatograms ; Sequencher program is needed, download here
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001184742
• ORF Size: 2016 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001184742.2
• RefSeq ORF: 2019 bp
• Locus ID: 10009
• UniProt ID: Q86T24
• Cytogenetics: Xq24
• MW: 74.9 kDa
• Summary: This gene encodes a transcriptional regulator with bimodal DNA-binding specificity, which binds to methylated CGCG and also to the non-methylated consensus KAISO-binding site TCCTGCNA. The protein contains an N-terminal POZ/BTB domain and 3 C-terminal zinc finger motifs. It recruits the N-CoR repressor complex to promote histone deacetylation and the formation of repressive chromatin structures in target gene promoters. It may contribute to the repression of target genes of the Wnt signaling pathway, and may also activate transcription of a subset of target genes by the recruitment of catenin delta-2 (CTNND2). Its interaction with catenin delta-1 (CTNND1) inhibits binding to both methylated and non-methylated DNA. It also interacts directly with the nuclear import receptor Importin-α2 (also known as karyopherin alpha2 or RAG cohort 1), which may mediate nuclear import of this protein. Alternatively spliced transcript variants encoding the same protein have been identified.[provided by RefSeq, May 2010]