ATAD3A (NM_001170535) Human Tagged ORF Clone

SKU RC230314

ATAD3A (Myc-DDK-tagged)-Human ATPase family, AAA domain containing 3A (ATAD3A), nuclear gene encoding mitochondrial protein, transcript variant 2

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: ATAD3A
• Synonyms: HAYOS; PHRINL
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC230314 representing NM_001170535; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGTCGTGGCTCTTCGGCATTAACAAGGGCCCCAAGGGTGAAGACGCGGGGCCGCCGCCGCCTTTGCCGC; CCGCGCAGCCCGGGGCCGAGGGCGGCGGGGACCGCGGCTTGGGAGACCGGCCGGCGCCCAAGGACAAATG; GAGCAACTTCGACCCCACCGGCCTGGAGCGCGCCGCCAAGGCGGCGCGCGAGCTGGAGCACTCGCGTTAT; GCCAAGGACGCCCTGAATCTGGCACAGATGCAGGAGCAGACGCTGCAGTTGGAGCAACAGTCCAAGCTCA; AAGAGTATGAGGCCGCCGTGGAGCAGCTCAAGAGCGAGCAGATCCGGGCGCAGGCTGAGGAGAGGAGGAA; GACCCTGAGCGAGGAGACCCGGCAGCACCAGGCCAGGGCCCAGTATCAAGACAAGCTGGCCCGGCAGCGC; TACGAGGACCAACTGAAGCAGCAGCAACTTCTCAATGAGGAGAATTTACGGAAGCAGGAGGAGTCCGTGC; AGAAGCAGGAAGCCATGCGGCGAGCCACCGTGGAGCGGGAGATGGAGCTGCGGCACAAGAATGAGATGCT; GCGAGTGGAGGCCGAGGCCCGGGCGCGCGCCAAGGCCGAGCGGGAGAATGCAGACATCATCCGCGAGCAG; ATCCGCCTGAAGGCGGCCGAGCACCGTCAGACCGTCTTGGAGTCCATCAGGACGGCTGGCACCTTGTTTG; GGGAAGGATTCCGTGCCTTTGTGACAGACTGGGACAAAGTGACAGCCACGGTGGCTGGGCTGACGCTGCT; GGCTGTTGGGGTCTACTCAGCCAAGAATGCCACGCTTGTCGCCGGCCGCTTCATCGAGGCTCGGCTGGGG; AAGCCGTCCCTAGTGAGGGAGACGTCCCGCATCACGGTGCTTGAGGCGCTGCGGCACCCCATCCAGGTCA; GCCGGCGGCTCCTCAGTCGACCCCAGGACGCGCTGGAGGGTGTTGTGCTCAGTCCCAGCCTGGAAGCACG; GGTGCGCGACATCGCCATAGCAACAAGGAACACCAAGAAGAACCGCAGCCTGTACAGGAACATCCTGATG; TACGGGCCACCAGGCACCGGGAAGACGCTGTTTGCCAAGAAACTCGCCCTGCACTCAGGCATGGACTACG; CCATCATGACAGGCGGGGACGTGGCCCCCATGGGGCGGGAAGGCGTGACCGCCATGCACAAGCTCTTTGA; CTGGGCCAATACCAGCCGGCGCGGCCTCCTGCTCTTTGTGGATGAAGCGGACGCCTTCCTTCGGAAGCGA; GCCACCGAGAAGATAAGCGAGGACCTCAGGGCCACACTGAACGCCTTCCTGTACCGCACGGGCCAGCACA; GCAACAAGTTCATGCTGGTCCTGGCCAGCAACCAACCAGAGCAGTTCGACTGGGCCATCAATGACCGCAT; CAATGAGATGGTCCACTTCGACCTGCCAGGGCAGGAGGAACGGGAGCGCCTGGTGAGAATGTATTTTGAC; AAGTATGTTCTTAAGCCGGCCACAGAAGGAAAGCAGCGCCTGAAGCTGGCCCAGTTTGACTACGGGAGGA; AGTGCTCGGAGGTCGCTCGGCTGACGGAGGGCATGTCGGGCCGGGAGATCGCTCAGCTGGCCGTGTCCTG; GCAGGCCACGGCGTATGCCTCCGAGGACGGGGTCCTGACCGAGGCCATGATGGACACCCGCGTGCAAGAT; GCTGTCCAGCAGCACCAGCAGAAGATGTGCTGGCTGAAGGCGGAAGGGCCTGGGCGTGGGGACGAGCCCT; CCCCATCC; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC230314 representing NM_001170535; Red=Cloning site Green=Tags(s); ; MSWLFGINKGPKGEDAGPPPPLPPAQPGAEGGGDRGLGDRPAPKDKWSNFDPTGLERAAKAARELEHSRY; AKDALNLAQMQEQTLQLEQQSKLKEYEAAVEQLKSEQIRAQAEERRKTLSEETRQHQARAQYQDKLARQR; YEDQLKQQQLLNEENLRKQEESVQKQEAMRRATVEREMELRHKNEMLRVEAEARARAKAERENADIIREQ; IRLKAAEHRQTVLESIRTAGTLFGEGFRAFVTDWDKVTATVAGLTLLAVGVYSAKNATLVAGRFIEARLG; KPSLVRETSRITVLEALRHPIQVSRRLLSRPQDALEGVVLSPSLEARVRDIAIATRNTKKNRSLYRNILM; YGPPGTGKTLFAKKLALHSGMDYAIMTGGDVAPMGREGVTAMHKLFDWANTSRRGLLLFVDEADAFLRKR; ATEKISEDLRATLNAFLYRTGQHSNKFMLVLASNQPEQFDWAINDRINEMVHFDLPGQEERERLVRMYFD; KYVLKPATEGKQRLKLAQFDYGRKCSEVARLTEGMSGREIAQLAVSWQATAYASEDGVLTEAMMDTRVQD; AVQQHQQKMCWLKAEGPGRGDEPSPS; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001170535
• ORF Size: 1758 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001170535.3
• RefSeq ORF: 1761 bp
• Locus ID: 55210
• UniProt ID: Q9NVI7
• Cytogenetics: 1p36.33
• MW: 66.7 kDa
• Summary: This gene encodes a ubiquitously expressed mitochondrial membrane protein that contributes to mitochondrial dynamics, nucleoid organization, protein translation, cell growth, and cholesterol metabolism. This gene is a member of the ATPase family AAA-domain containing 3 gene family which, in humans, includes two other paralogs. Naturally occurring mutations in this gene are associated with distinct neurological syndromes including Harel-Yoon syndrome. High-level expression of this gene is associated with poor survival in breast cancer patients. A homozygous knockout of the orthologous gene in mice results in embryonic lethality at day 7.5 due to growth retardation and defective development of the trophoblast lineage. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2017]