HES7 (NM_001165967) Human Tagged ORF Clone

SKU
RC228163
HES7 (Myc-DDK-tagged)-Human hairy and enhancer of split 7 (Drosophila) (HES7), transcript variant 1
  • TrueORF®
    TrueORF®

    Expression-ready ORF plasmid with C-terminal tag(s)

    Click here to learn more.

$330.00
3 Weeks*
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol HES7
Synonyms bHLHb37; SCDO4
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC228163 representing NM_001165967
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGTCACCCGGGATCGAGCTGAGAATAGGGACGGCCCCAAGATGCTCAAGCCGCTTGTGGAGAAGCGGC
GCCGGGACCGCATCAACCGCAGCCTGGAAGAGCTGAGGCTGCTGCTGCTGGAGCGGACCCGGGACCAGAA
CCTCCGGAACCCGAAGCTGGAGAAAGCGGAGATATTGGAGTTCGCCGTGGGCTACTTGAGGGAGCGAAGC
CGGGTGGAGCCCCCGGCCGCCGCGGCTCCAGGGGTTCCCCGGTCCCCAGTCCAGGACGCCGAGGCGCTCG
CCAGCTGCTACTTGTCCGGTTTCCGCGAGTGCCTGCTTCGCTTGGCGGCCTTCGCGCACGACGCCAGCCC
GGCCGCCCGCGCCCAGCTCTTCTCCGCGCTGCACGGCTATCTGCGCCCCAAACCGCCCCGGCCCAAGCCG
GTAGATCCGAGGCCTCCAGCGCCGCGCCCATCCCTGGACCCCGCCGCACCGGCCCTTGGCCCTGCGCTGC
ACCAGCGCCCCCCAGTGCACCAGGGCCACCCTAGCCCGCGCTGCGCATGGTCCCCATCCCTCTGCTCCCC
GCGCGCCGGGGATTCTGGCGCGCCGGCGCCCCTCACCGGACTGCTGCCGCCGCCACCGCCGCCTCACAGA
CAAGACGGGGCGCCCAAGGCCCCGCTGCCCCCGCCGCCCGCTTTCTGGAGACCTTGGCCC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC228163 representing NM_001165967
Red=Cloning site Green=Tags(s)

MVTRDRAENRDGPKMLKPLVEKRRRDRINRSLEELRLLLLERTRDQNLRNPKLEKAEILEFAVGYLRERS
RVEPPAAAAPGVPRSPVQDAEALASCYLSGFRECLLRLAAFAHDASPAARAQLFSALHGYLRPKPPRPKP
VDPRPPAPRPSLDPAAPALGPALHQRPPVHQGHPSPRCAWSPSLCSPRAGDSGAPAPLTGLLPPPPPPHR
QDGAPKAPLPPPPAFWRPWP

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene Plasmid Map
ACCN NM_001165967
ORF Size 690 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001165967.2
RefSeq ORF 693 bp
Locus ID 84667
UniProt ID F8VPC9
Cytogenetics 17p13.1
MW 25.1 kDa
Summary This gene encodes a member of the hairy and enhancer of split family of bHLH transcription factors. The mouse ortholog of this gene is regulated by Notch signaling. The protein functions as a transcriptional repressor, and is implicated in correct patterning of the axial skeleton. A mutation in this gene has been shown to result in spondylocostal dysostosis. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Sep 2009]
Write Your Own Review
You're reviewing:HES7 (NM_001165967) Human Tagged ORF Clone
Your Rating
SKU Description Size Price
RC228163L3 Lenti-ORF clone of HES7 (Myc-DDK-tagged)-Human hairy and enhancer of split 7 (Drosophila) (HES7), transcript variant 1 10 ug
$630.00
RC228163L4 Lenti-ORF clone of HES7 (mGFP-tagged)-Human hairy and enhancer of split 7 (Drosophila) (HES7), transcript variant 1 10 ug
$630.00
RG228163 HES7 (tGFP-tagged) - Human hairy and enhancer of split 7 (Drosophila) (HES7), transcript variant 1 10 ug
$530.00
SC326798 HES7 (untagged)-Human hairy and enhancer of split 7 (Drosophila) (HES7) transcript variant 1 10 ug
$300.00

Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.