Bestrophin (BEST1) (NM_001139443) Human Tagged ORF Clone

SKU RC227442

BEST1 (Myc-DDK-tagged)-Human bestrophin 1 (BEST1), transcript variant 2

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: Bestrophin
• Synonyms: ARB; BEST; Best1V1Delta2; BMD; RP50; TU15B; VMD2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC227442 representing NM_001139443; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGTTTGAGAAACTGACTCTGTATTGCGACAGCTACATCCAGCTCATCCCCATTTCCTTCGTGCTGGGCT; TCTACGTGACGCTGGTCGTGACCCGCTGGTGGAACCAGTACGAGAACCTGCCGTGGCCCGACCGCCTCAT; GAGCCTGGTGTCGGGCTTCGTCGAAGGCAAGGACGAGCAAGGCCGGCTGCTGCGGCGCACGCTCATCCGC; TACGCCAACCTGGGCAACGTGCTCATCCTGCGCAGCGTCAGCACCGCAGTCTACAAGCGCTTCCCCAGCG; CCCAGCACCTGGTGCAAGCAGGCTTTATGACTCCGGCAGAACACAAGCAGTTGGAGAAACTGAGCCTACC; ACACAACATGTTCTGGGTGCCCTGGGTGTGGTTTGCCAACCTGTCAATGAAGGCGTGGCTTGGAGGTCGA; ATCCGGGACCCTATCCTGCTCCAGAGCCTGCTGAACGAGATGAACACCTTGCGTACTCAGTGTGGACACC; TGTATGCCTACGACTGGATTAGTATCCCACTGGTGTATACACAGGTGGTGACTGTGGCGGTGTACAGCTT; CTTCCTGACTTGTCTAGTTGGGCGGCAGTTTCTGAACCCAGCCAAGGCCTACCCTGGCCATGAGCTGGAC; CTCGTTGTGCCCGTCTTCACGTTCCTGCAGTTCTTCTTCTATGTTGGCTGGCTGAAGGTGGCAGAGCAGC; TCATCAACCCCTTTGGAGAGGATGATGATGATTTTGAGACCAACTGGATTGTCGACAGGAATTTGCAGGT; GTCCCTGTTGGCTGTGGATGAGATGCACCAGGACCTGCCTCGGATGGAGCCGGACATGTACTGGAATAAG; CCCGAGCCACAGCCCCCCTACACAGCTGCTTCCGCCCAGTTCCGTCGAGTCTCCTTTATGGGCTCCACCT; TCAACATCAGCCTGAACAAAGAGGAGATGGAGTTCCAGCCCAATCAGGAGGACGAGGAGGATGCTCACGC; TGGCATCATTGGCCGCTTCCTAGGCCTGCAGTCCCATGATCACCATCCTCCCAGGGCAAACTCAAGGACC; AAACTACTGTGGCCCAAGAGGGAATCCCTTCTCCACGAGGGCCTGCCCAAAAACCACAAGGCAGCCAAAC; AGAACGTTAGGGGCCAGGAAGACAACAAGGCCTGGAAGCTTAAGGCTGTGGACGCCTTCAAGTCTGCCCC; ACTGTATCAGAGGCCAGGCTACTACAGTGCCCCACAGACGCCCCTCAGCCCCACTCCCATGTTCTTCCCC; CTAGAACCATCAGCGCCGTCAAAGCTTCACAGTGTCACAGGCATAGACACCAAAGACAAAAGCTTAAAGA; CTGTGAGTTCTGGGGCCAAAAAAAGTTTTGAATTGCTCTCAGAGAGCGATGGGGCCTTGATGGAGCACCC; AGAAGTATCTCAAGTGAGGAGGAAAACTGTGGAGTTTAACCTGACGGATATGCCAGAGATCCCCGAAAAT; CACCTCAAAGAACCTTTGGAACAATCACCAACCAACATACACACTACACTCAAAGATCACATGGATCCTT; ATTGGGCCTTGGAAAACAGGTCTGTCCTCCACCTGAACCAGGGGCACTGCATTGCCCTGTGCCCCACCCC; AGCTTCCCTTGCTCTGAGCCTACCCTTCCTCCACAATTTCCTAGGGTTCCATCACTGCCAGAGCACACTG; GACCTACGCCCAGCACTGGCTTGGGGTATATACTTGGCCACCTTCACAGGGATCCTAGGGAAGTGTTCGG; GACCTTTTCTCACTTCACCCTGGTATCACCCGGAAGACTTCTTGGGACCAGGTGAAGGAAGA; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC227442 representing NM_001139443; Red=Cloning site Green=Tags(s); ; MFEKLTLYCDSYIQLIPISFVLGFYVTLVVTRWWNQYENLPWPDRLMSLVSGFVEGKDEQGRLLRRTLIR; YANLGNVLILRSVSTAVYKRFPSAQHLVQAGFMTPAEHKQLEKLSLPHNMFWVPWVWFANLSMKAWLGGR; IRDPILLQSLLNEMNTLRTQCGHLYAYDWISIPLVYTQVVTVAVYSFFLTCLVGRQFLNPAKAYPGHELD; LVVPVFTFLQFFFYVGWLKVAEQLINPFGEDDDDFETNWIVDRNLQVSLLAVDEMHQDLPRMEPDMYWNK; PEPQPPYTAASAQFRRVSFMGSTFNISLNKEEMEFQPNQEDEEDAHAGIIGRFLGLQSHDHHPPRANSRT; KLLWPKRESLLHEGLPKNHKAAKQNVRGQEDNKAWKLKAVDAFKSAPLYQRPGYYSAPQTPLSPTPMFFP; LEPSAPSKLHSVTGIDTKDKSLKTVSSGAKKSFELLSESDGALMEHPEVSQVRRKTVEFNLTDMPEIPEN; HLKEPLEQSPTNIHTTLKDHMDPYWALENRSVLHLNQGHCIALCPTPASLALSLPFLHNFLGFHHCQSTL; DLRPALAWGIYLATFTGILGKCSGPFLTSPWYHPEDFLGPGEGR; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Chromatograms: Chromatograms ; Sequencher program is needed, download here
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001139443
• ORF Size: 1812 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001139443.1, NP_001132915.1
• RefSeq ORF: 1815 bp
• Locus ID: 7439
• UniProt ID: O76090
• Cytogenetics: 11q12.3
• Protein Families: Druggable Genome, Ion Channels: Other, Transmembrane
• MW: 68.9 kDa
• Summary: This gene encodes a member of the bestrophin gene family. This small gene family is characterized by proteins with a highly conserved N-terminus with four to six transmembrane domains. Bestrophins may form chloride ion channels or may regulate voltage-gated L-type calcium-ion channels. Bestrophins are generally believed to form calcium-activated chloride-ion channels in epithelial cells but they have also been shown to be highly permeable to bicarbonate ion transport in retinal tissue. Mutations in this gene are responsible for juvenile-onset vitelliform macular dystrophy (VMD2), also known as Best macular dystrophy, in addition to adult-onset vitelliform macular dystrophy (AVMD) and other retinopathies. Alternative splicing results in multiple variants encoding distinct isoforms.[provided by RefSeq, Nov 2008]