PLEKHG4 (NM_001129729) Human Tagged ORF Clone

SKU
RC226355
PLEKHG4 (Myc-DDK-tagged)-Human pleckstrin homology domain containing, family G (with RhoGef domain) member 4 (PLEKHG4), transcript variant 4
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    TrueORF®

    Expression-ready ORF plasmid with C-terminal tag(s)

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$589.00 MSRP $1,037.00 MSRP $1,037.00
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Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol PLEKHG4
Synonyms ARHGEF44; PRTPHN1; SCA4
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC226355 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAAAGGCCCCTGGAGAATGGGGATGAGTCCCCAGACTCTCAGGGCCATGCCACCGACTGGAGATTTG
CTGTGTGCAGTTTCAGGGATGCCTGGGAAGAGGAGGAACCTGCTTCCCAGATGCACGTTAAGGACCCAGG
TCCTCCAAGACCACCAGCCGGGGCCACCCAGGATGAGGAGCTACAGGGCAGCCCCTTGTCCAGGAAATTC
CAGTTACCCCCAGCTGCAGATGAGTCGGGGGATGCCCAGAGGGGCACAGTAGAAAGCTCCTCAGTCCTGT
CAGAAGGGCCAGGCCCCTCTGGAGTGGAGAGTCTCCTATGCCCCATGTCCTCCCACCTCAGCTTGGCACA
GGGTGAGAGTGACACCCCAGGGGTAGGGTTGGTAGGGGACCCAGGTCCAAGCAGGGCGATGCCATCTGGC
TTGAGCCCTGGGGCATTGGACAGCGACCCTGTGGGCCTTGGAGACCCTTTATCAGAAATATCAAAGCTGC
TGGAGGCAGCCCCCAGTGGATCCGGCCTCCCTAAGCCTGCTGACTGCCTCCTGGCCCAAGACCTCTGTTG
GGAGCTGCTGGCCAGTGGTATGGCCACCTTGCCAGGGACTCGGGATGTCCAAGGCCGGGCAGTGCTGCTT
CTGTGTGCCCACAGCCCAGCCTGGCTTCAGTCTGAGTGCAGCAGCCAGGAACTCATCCGCCTCCTGCTGT
ACCTGCGAAGCATCCCCAGGCCCGAAGTACAGGCACTGGGACTGACAGTGCTAGTTGATGCCCGAATTTG
TGCTCCAAGTTCTTCCCTCTTCTCTGGGCTCAGCCAACTACAAGAAGCAGCCCCAGGGGCCGTGTACCAG
GTGCTGCTAGTGGGAAGCACGCTGCTGAAGGAAGTGCCTTCCGGGCTGCAGCTGGAGCAGTTGCCTTCTC
AGAGCCTGCTGACCCACATCCCAACGGCGGGGCTGCCCACTTCGCTAGGAGGAGGCCTGCCTTACTGCCA
CCAGGCCTGGCTGGATTTCCGAAGGCGGCTGGAAGCTCTACTACAGAACTGCCAGGCAGCTTGTGCCCTG
CTCCAGGGGGCCATCGAAAGTGTGAAGGCTGTGCCCCAGCCCATGGAGCCTGGGGAGGTCGGTCAGCTGC
TACAGCAGACAGAGGTCCTGATGCAGCAGGTGCTAGACTCGCCATGGCTGGCATGGCTACAATGCCAGGG
GGGCCGGGAGCTGACATGGCTGAAGCAAGAGGTCCCAGAGGTGACCCTGAGCCCAGACTACAGGACGGCA
ATGGACAAGGCTGACGAGCTATATGACCGGGTGGATGGATTGCTGCACCAACTGACCCTGCAGAGCAACC
AGCGAATACAGGCCCTAGAGTTGGTCCAAACACTGGAGGCCCGGGAAAGCGGACTGCACCAGATTGAAGT
GTGGCTGCAGCAGGTGGGCTGGCCAGCACTGGAGGAGGCTGGGGAGCCCTCGCTGGACATGCTGCTCCAG
GCCCAAGGCTCTTTTCAGGAGCTGTACCAGGTTGCCCAGGAGCAGGTCAGGCAAGGGGAGAAGTTTCTGC
AGCCGCTGACTGGCTGGGAGGCGGCTGAACTGGACCCCCCTGGGGCACGCTTTCTGGCCCTGCGAGCCCA
GCTGACTGAATTCTCTAGGGCTTTGGCCCAGCGGTGCCAGCGGCTGGCGGATGCTGAGAGGCTGTTTCAG
CTCTTCAGGGAGGCCTTGACGTGGGCTGAGGAGGGGCAGCGAGTGTTGGCAGAGCTGGAGCAGGAACGCC
CGGGGGTTGTGTTGCAGCAGCTGCAGCTGCACTGGACCAGGCACCCTGACTTGCCTCCTGCCCACTTCCG
AAAGATGTGGGCTCTGGCCACGGGGCTGGGCTCAGAGGCCATCCGCCAGGAGTGCCGCTGGGCCTGGGCG
CGGTGCCAGGACACCTGGCTGGCCCTGGACCAAAAGCTTGAGGCTTCACTGAAGCTACCACCGGTGGGCA
GCACAGCTAGCCTGTGTGTCAGCCAGGTCCCCGCTGCACCTGCCCACCCTCCCCTGAGGAAGGCCTACAG
CTTCGATCGGAATCTGGGGCAGAGTCTCAGTGAACCTGCCTGCCACTGCCACCATGCGGCCACTATTGCT
GCCTGCCGCAGACCAGAGGCTGGAGGAGGTGCCCTGCCCCAGGCATCCCCTACTGTGCCTCCACCAGGCA
GCTCTGACCCCAGGAGCCTCAACAGGCTACAGCTGGTGCTGGCAGAGATGGTGGCCACGGAGCGGGAGTA
TGTCCGGGCTCTAGAGTACACTATGGAGAACTATTTCCCCGAGCTGGATCGCCCCGATGTGCCCCAGGGC
CTCCGCGGTCAGCGTGCCCACCTCTTTGGCAACCTGGAGAAGCTGCGGGACTTCCACTGCCACTTCTTCC
TGCGTGAGCTGGAGGCTTGCACCCGGCACCCACCACGAGTGGCCTATGCCTTCCTGCGCCATAGGGTGCA
GTTTGGGATGTACGCGCTCTACAGCAAGAATAAGCCTCGCTCCGATGCCCTGATGTCAAGCTATGGGCAC
ACCTTCTTCAAGGACAAGCAGCAAGCACTGGGGGACCACCTGGACCTGGCCTCCTACCTGCTAAAGCCCA
TCCAGCGCATGGGCAAGTACGCACTGCTGCTGCAGGAGCTGGCACGGGCCTGCGGGGGCCCCACGCAGGA
GCTCAGTGCGCTGCGGGAGGCCCAGAGCCTTGTGCACTTCCAGCTGCGGCACGGAAACGACCTGCTGGCC
ATGGACGCCATCCAGGGCTGTGATGTTAACCTCAAGGAACAGGGGCAGCTGGTGCGACAGGATGAGTTTG
TGGTGCGCACTGGGCGCCACAAGTCCGTGCGCCGCATCTTCCTTTTTGAGGAGCTGCTGCTCTTCAGCAA
GCCTCGCCATGGGCCCACAGGGGTTGACACATTTGCCTACAAGCGCTCCTTCAAGATGGCAGACCTTGGT
CTCACTGAGTGCTGTGGGAACAGCAACCTGCGCTTCGAGATCTGGTTCCGCCGCCGCAAGGCCAGGGACA
CCTTTGTGCTGCAGGCCTCCAGCCTGGCTATCAAGCAGGCCTGGACAGCTGACATCTCCCACCTGCTTTG
GAGGCAGGCCGTCCACAACAAGGAGGTGCGCATGGCTGAGATGGTGTCCATGGGTGTGGGGAACAAGGCC
TTCCGAGACATTGCTCCCAGCGAGGAAGCCATCAACGACCGCACCGTCAACTATGTCCTGAAGTGCCGAG
AAGTTCGCTCTCGGGCGTCCATTGCCGTAGCCCCGTTTGACCATGACAGCCTCTACCTGGGGGCCTCGAA
CTCCCTTCCTGGAGACCCTGCCTCTTGCTCTGTTCTGGGGTCCCTCAACCTGCACCTGTACAGAGACCCA
GCTCTTCTGGGTCTCCGCTGTCCCCTGTATCCCAGCTTCCCAGAGGAAGCAGCACTGGAGGCTGAGGCAG
AGCTGGGCGGCCAGCCCTCTTTGACTGCTGAGGACTCAGAGATCTCGTCCCAATGCCCATCAGCCAGTGG
CTCCAGTGGCTCTGACAGCAGCTGTGTGTCAGGGCAGGCCCTGGGTAGGGGCCTGGAGGACTTACCCTGT
GTC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC226355 protein sequence
Red=Cloning site Green=Tags(s)

MERPLENGDESPDSQGHATDWRFAVCSFRDAWEEEEPASQMHVKDPGPPRPPAGATQDEELQGSPLSRKF
QLPPAADESGDAQRGTVESSSVLSEGPGPSGVESLLCPMSSHLSLAQGESDTPGVGLVGDPGPSRAMPSG
LSPGALDSDPVGLGDPLSEISKLLEAAPSGSGLPKPADCLLAQDLCWELLASGMATLPGTRDVQGRAVLL
LCAHSPAWLQSECSSQELIRLLLYLRSIPRPEVQALGLTVLVDARICAPSSSLFSGLSQLQEAAPGAVYQ
VLLVGSTLLKEVPSGLQLEQLPSQSLLTHIPTAGLPTSLGGGLPYCHQAWLDFRRRLEALLQNCQAACAL
LQGAIESVKAVPQPMEPGEVGQLLQQTEVLMQQVLDSPWLAWLQCQGGRELTWLKQEVPEVTLSPDYRTA
MDKADELYDRVDGLLHQLTLQSNQRIQALELVQTLEARESGLHQIEVWLQQVGWPALEEAGEPSLDMLLQ
AQGSFQELYQVAQEQVRQGEKFLQPLTGWEAAELDPPGARFLALRAQLTEFSRALAQRCQRLADAERLFQ
LFREALTWAEEGQRVLAELEQERPGVVLQQLQLHWTRHPDLPPAHFRKMWALATGLGSEAIRQECRWAWA
RCQDTWLALDQKLEASLKLPPVGSTASLCVSQVPAAPAHPPLRKAYSFDRNLGQSLSEPACHCHHAATIA
ACRRPEAGGGALPQASPTVPPPGSSDPRSLNRLQLVLAEMVATEREYVRALEYTMENYFPELDRPDVPQG
LRGQRAHLFGNLEKLRDFHCHFFLRELEACTRHPPRVAYAFLRHRVQFGMYALYSKNKPRSDALMSSYGH
TFFKDKQQALGDHLDLASYLLKPIQRMGKYALLLQELARACGGPTQELSALREAQSLVHFQLRHGNDLLA
MDAIQGCDVNLKEQGQLVRQDEFVVRTGRHKSVRRIFLFEELLLFSKPRHGPTGVDTFAYKRSFKMADLG
LTECCGNSNLRFEIWFRRRKARDTFVLQASSLAIKQAWTADISHLLWRQAVHNKEVRMAEMVSMGVGNKA
FRDIAPSEEAINDRTVNYVLKCREVRSRASIAVAPFDHDSLYLGASNSLPGDPASCSVLGSLNLHLYRDP
ALLGLRCPLYPSFPEEAALEAEAELGGQPSLTAEDSEISSQCPSASGSSGSDSSCVSGQALGRGLEDLPC
V

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene Plasmid Map
ACCN NM_001129729
ORF Size 3573 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001129729.3
RefSeq Size 4493 bp
RefSeq ORF 3576 bp
Locus ID 25894
UniProt ID Q58EX7
Cytogenetics 16q22.1
MW 130.8 kDa
Summary The protein encoded by this gene can function as a guanine nucleotide exchange factor (GEF) and may play a role in intracellular signaling and cytoskeleton dynamics at the Golgi apparatus. Polymorphisms in the region of this gene have been found to be associated with spinocerebellar ataxia in some study populations. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2015]
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SKU Description Size Price
RC226355L3 Lenti-ORF clone of PLEKHG4 (Myc-DDK-tagged)-Human pleckstrin homology domain containing, family G (with RhoGef domain) member 4 (PLEKHG4), transcript variant 4 10 ug
$1,337.00
RC226355L4 Lenti-ORF clone of PLEKHG4 (mGFP-tagged)-Human pleckstrin homology domain containing, family G (with RhoGef domain) member 4 (PLEKHG4), transcript variant 4 10 ug
$1,337.00
RG226355 PLEKHG4 (tGFP-tagged) - Human pleckstrin homology domain containing, family G (with RhoGef domain) member 4 (PLEKHG4), transcript variant 4 10 ug
$1,237.00
SC323265 PLEKHG4 (untagged)-Human pleckstrin homology domain containing, family G (with RhoGef domain) member 4 (PLEKHG4), transcript variant 4 10 ug
$1,141.00

Citations

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