Neurofilament (NEFM) (NM_005382) Human Tagged ORF Clone

SKU RC224475

NEFM (Myc-DDK-tagged)-Human neurofilament, medium polypeptide (NEFM), transcript variant 1

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: Neurofilament
• Synonyms: NEF3; NF-M; NFM
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC224475 representing NM_005382; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGAGCTACACGTTGGACTCGCTGGGCAACCCGTCCGCCTACCGGCGGGTAACCGAGACCCGCTCGAGCT; TCAGCCGCGTCAGCGGCTCCCCGTCCAGTGGCTTCCGCTCGCAGTCGTGGTCCCGCGGCTCGCCCAGCAC; CGTGTCCTCCTCCTATAAGCGCAGCATGCTCGCCCCGCGCCTCGCTTACAGCTCGGCCATGCTCAGCTCC; GCCGAGAGCAGCCTTGACTTCAGCCAGTCCTCGTCCCTGCTCAACGGCGGCTCCGGACCCGGCGGCGACT; ACAAGCTGTCCCGCTCCAACGAGAAGGAGCAGCTGCAGGGGCTGAACGACCGCTTTGCCGGCTACATAGA; GAAGGTGCACTACCTGGAGCAGCAGAATAAGGAGATTGAGGCGGAGATCCAGGCGCTGCGGCAGAAGCAG; GCCTCGCACGCCCAGCTGGGCGACGCGTACGACCAGGAGATCCGCGAGCTGCGCGCCACCCTGGAGATGG; TGAACCACGAGAAGGCTCAGGTGCAGCTGGACTCGGACCACCTGGAGGAAGACATCCACCGGCTCAAGGA; GCGCTTTGAGGAGGAGGCGCGGTTGCGCGACGACACTGAGGCGGCCATCCGCGCGCTGCGCAAAGACATC; GAGGAGGCGTCGCTGGTCAAGGTGGAGCTGGACAAGAAGGTGCAGTCGCTGCAGGATGAGGTGGCCTTCC; TGCGGAGCAACCACGAGGAGGAGGTGGCCGACCTTCTGGCCCAGATCCAGGCATCGCACATCACGGTGGA; GCGCAAAGACTACCTGAAGACAGACATCTCGACGGCGCTGAAGGAAATCCGCTCCCAGCTCGAAAGCCAC; TCAGACCAGAATATGCACCAGGCCGAAGAGTGGTTCAAATGCCGCTACGCCAAGCTCACCGAGGCGGCCG; AGCAGAACAAGGAGGCCATCCGCTCCGCCAAGGAAGAGATCGCCGAGTACCGGCGCCAGCTGCAGTCCAA; GAGCATCGAGCTAGAGTCGGTGCGCGGCACCAAGGAGTCCCTGGAGCGGCAGCTCAGCGACATCGAGGAG; CGCCACAACCACGACCTCAGCAGCTACCAGGACACCATCCAGCAGCTGGAAAATGAGCTTCGGGGCACAA; AGTGGGAAATGGCTCGTCATTTGCGCGAATACCAGGACCTCCTCAACGTCAAGATGGCTCTGGATATAGA; AATCGCTGCGTACAGAAAACTCCTGGAGGGTGAAGAGACTAGATTTAGCACATTTGCAGGAAGCATCACT; GGGCCACTGTATACACACCGACCCCCAATCACAATATCCAGTAAGATTCAGAAAACCAAGGTGGAAGCTC; CCAAGCTTAAGGTCCAACACAAATTTGTCGAGGAGATCATAGAGGAAACCAAAGTGGAGGATGAGAAGTC; AGAAATGGAAGAGGCCCTGACAGCCATTACAGAGGAATTGGCCGTTTCCATGAAGGAAGAGAAGAAAGAA; GCAGCAGAAGAAAAGGAAGAGGAACCCGAAGCTGAAGAAGAAGAAGTAGCTGCCAAAAAGTCTCCAGTGA; AAGCAACTGCACCTGAAGTTAAAGAAGAGGAAGGGGAAAAGGAGGAAGAAGAAGGCCAGGAAGAAGAGGA; GGAAGAAGATGAGGGAGCTAAGTCAGACCAAGCCGAAGAGGGAGGATCCGAGAAGGAAGGCTCTAGTGAA; AAAGAGGAAGGTGAGCAGGAAGAAGGAGAAACAGAAGCTGAAGCTGAAGGAGAGGAAGCCGAAGCTAAAG; AGGAAAAGAAAGTGGAGGAAAAGAGTGAGGAAGTGGCTACCAAGGAGGAGCTGGTGGCAGATGCCAAGGT; GGAAAAGCCAGAAAAAGCCAAGTCTCCTGTGCCAAAATCACCAGTGGAAGAGAAAGGCAAGTCTCCTGTG; CCCAAGTCACCAGTGGAAGAGAAAGGCAAGTCTCCTGTGCCCAAGTCACCAGTGGAAGAGAAAGGCAAGT; CTCCTGTGCCGAAATCACCAGTGGAAGAGAAAGGCAAGTCTCCTGTGTCAAAATCACCAGTGGAAGAGAA; AGCCAAATCTCCTGTGCCAAAATCACCAGTGGAAGAGGCAAAGTCAAAAGCAGAAGTGGGGAAAGGTGAA; CAGAAAGAGGAAGAAGAAAAGGAAGTCAAGGAAGCTCCCAAGGAAGAGAAGGTAGAGAAAAAGGAAGAGA; AACCAAAGGATGTGCCAGAGAAGAAGAAAGCTGAGTCCCCTGTAAAGGAGGAAGCTGTGGCAGAGGTGGT; CACCATCACCAAATCGGTAAAGGTGCACTTGGAGAAAGAGACCAAAGAAGAGGGGAAGCCACTGCAGCAG; GAGAAAGAGAAGGAGAAAGCGGGAGGAGAGGGAGGAAGTGAGGAGGAAGGGAGTGATAAAGGTGCCAAGG; GATCCAGGAAGGAAGACATAGCTGTCAATGGGGAGGTAGAAGGAAAAGAGGAGGTAGAGCAGGAGACCAA; GGAAAAAGGCAGTGGGAGGGAAGAGGAGAAAGGCGTTGTCACCAATGGCCTAGACTTGAGCCCAGCAGAT; GAAAAGAAGGGGGGTGATAAAAGTGAGGAGAAAGTGGTGGTGACCAAAACGGTAGAAAAAATCACCAGTG; AGGGGGGAGATGGTGCTACCAAATACATCACTAAATCTGTAACCGTCACTCAAAAGGTTGAAGAGCATGA; AGAGACCTTTGAGGAGAAACTAGTGTCTACTAAAAAGGTAGAAAAAGTCACTTCACACGCCATAGTAAAG; GAAGTCACCCAGAGTGAC; ; AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC; TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC224475 representing NM_005382; Red=Cloning site Green=Tags(s); ; MSYTLDSLGNPSAYRRVTETRSSFSRVSGSPSSGFRSQSWSRGSPSTVSSSYKRSMLAPRLAYSSAMLSS; AESSLDFSQSSSLLNGGSGPGGDYKLSRSNEKEQLQGLNDRFAGYIEKVHYLEQQNKEIEAEIQALRQKQ; ASHAQLGDAYDQEIRELRATLEMVNHEKAQVQLDSDHLEEDIHRLKERFEEEARLRDDTEAAIRALRKDI; EEASLVKVELDKKVQSLQDEVAFLRSNHEEEVADLLAQIQASHITVERKDYLKTDISTALKEIRSQLESH; SDQNMHQAEEWFKCRYAKLTEAAEQNKEAIRSAKEEIAEYRRQLQSKSIELESVRGTKESLERQLSDIEE; RHNHDLSSYQDTIQQLENELRGTKWEMARHLREYQDLLNVKMALDIEIAAYRKLLEGEETRFSTFAGSIT; GPLYTHRPPITISSKIQKTKVEAPKLKVQHKFVEEIIEETKVEDEKSEMEEALTAITEELAVSMKEEKKE; AAEEKEEEPEAEEEEVAAKKSPVKATAPEVKEEEGEKEEEEGQEEEEEEDEGAKSDQAEEGGSEKEGSSE; KEEGEQEEGETEAEAEGEEAEAKEEKKVEEKSEEVATKEELVADAKVEKPEKAKSPVPKSPVEEKGKSPV; PKSPVEEKGKSPVPKSPVEEKGKSPVPKSPVEEKGKSPVSKSPVEEKAKSPVPKSPVEEAKSKAEVGKGE; QKEEEEKEVKEAPKEEKVEKKEEKPKDVPEKKKAESPVKEEAVAEVVTITKSVKVHLEKETKEEGKPLQQ; EKEKEKAGGEGGSEEEGSDKGAKGSRKEDIAVNGEVEGKEEVEQETKEKGSGREEEKGVVTNGLDLSPAD; EKKGGDKSEEKVVVTKTVEKITSEGGDGATKYITKSVTVTQKVEEHEETFEEKLVSTKKVEKVTSHAIVK; EVTQSD; ; SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005382
• ORF Size: 2748 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_005382.2
• RefSeq Size: 3238 bp
• RefSeq ORF: 2751 bp
• Locus ID: 4741
• UniProt ID: P07197
• Cytogenetics: 8p21.2
• Domains: filament, filament_head
• Protein Pathways: Amyotrophic lateral sclerosis (ALS)
• MW: 102.3 kDa
• Summary: Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and functionally maintain neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the medium neurofilament protein. This protein is commonly used as a biomarker of neuronal damage. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Oct 2008]