ERCC6L (NM_017669) Human Tagged ORF Clone

SKU RC223966

ERCC6L (Myc-DDK-tagged)-Human excision repair cross-complementing rodent repair deficiency, complementation group 6-like (ERCC6L)

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: ERCC6L
• Synonyms: PICH; RAD26L
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC223966 representing NM_017669; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGAGGCATCCCGAAGGTTTCCGGAAGCCGAGGCCTTGAGCCCAGAGCAGGCTGCTCATTACCTAAGAT; ATGTGAAAGAGGCCAAAGAAGCAACTAAGAATGGAGACCTGGAAGAAGCATTTAAACTTTTCAATTTGGC; AAAGGACATTTTTCCCAATGAAAAAGTGCTGAGCAGAATCCAAAAAATACAGGAAGCCTTGGAGGAGTTG; GCAGAACAGGGAGATGATGAATTTACAGATGTGTGCAACTCTGGCTTGCTACTTTATCGAGAACTGCACA; ACCAACTCTTTGAGCACCAGAAGGAAGGCATAGCTTTCCTCTATAGCCTGTATAGGGATGGAAGAAAAGG; TGGTATATTGGCTGATGATATGGGATTAGGGAAGACTGTTCAAATCATTGCTTTCCTTTCCGGTATGTTT; GATGCATCACTTGTGAATCATGTGCTGCTGATCATGCCAACCAATCTTATTAACACATGGGTAAAAGAAT; TCATCAAGTGGACTCCAGGAATGAGAGTCAAAACCTTTCATGGTCCTAGCAAGGATGAACGGACCAGAAA; CCTCAATCGGATTCAGCAAAGGAATGGTGTTATTATCACTACATACCAAATGTTAATCAATAACTGGCAG; CAACTTTCAAGCTTTAGGGGCCAAGAGTTTGTGTGGGACTATGTCATCCTCGATGAAGCACATAAAATAA; AAACCTCATCTACTAAGTCAGCAATATGTGCTCGTGCTATTCCTGCAAGTAATCGCCTCCTCCTCACAGG; AACCCCAATCCAGAATAATTTACAAGAACTATGGTCCCTATTTGATTTTGCTTGTCAAGGGTCCCTGCTG; GGAACATTAAAAACTTTTAAGATGGAGTATGAAAATCCTATTACTAGAGCAAGAGAGAAGGATGCTACCC; CAGGAGAAAAAGCCTTGGGATTTAAAATATCTGAAAACTTAATGGCAATCATAAAACCCTATTTTCTCAG; GAGGACTAAAGAAGACGTACAGAAGAAAAAGTCAAGCAACCCAGAGGCCAGACTTAATGAAAAGAATCCA; GATGTTGATGCCATTTGTGAAATGCCTTCCCTTTCCAGGAAAAATGATTTAATTATTTGGATACGACTTG; TGCCTTTACAAGAAGAAATATACAGGAAATTTGTGTCTTTAGATCATATCAAGGAGTTGCTAATGGAGAC; GCGCTCACCTTTGGCTGAGCTAGGTGTCTTAAAGAAGCTGTGTGATCATCCTAGGCTGCTGTCTGCACGG; GCTTGTTGTTTGCTAAATCTTGGGACATTCTCTGCTCAAGATGGAAATGAGGGGGAAGATTCCCCAGATG; TGGACCATATTGATCAAGTAACTGATGACACATTGATGGAAGAATCTGGAAAAATGATATTCCTAATGGA; CCTACTTAAGAGGCTGCGAGATGAGGGACATCAAACTCTGGTGTTTTCTCAATCGAGGCAAATTCTAAAC; ATCATTGAACGCCTCTTAAAGAATAGGCACTTTAAGACATTGCGAATCGATGGGACAGTTACTCATCTTT; TGGAACGAGAAAAAAGAATTAACTTATTCCAGCAAAATAAAGATTACTCTGTTTTTCTGCTTACCACTCA; AGTAGGTGGTGTCGGTTTAACATTAACTGCAGCAACTAGAGTGGTCATTTTTGACCCTAGCTGGAATCCT; GCAACTGATGCTCAAGCTGTGGATAGAGTTTACCGAATTGGACAAAAAGAGAATGTTGTGGTTTATAGGC; TAATCACTTGTGGGACTGTAGAGGAAAAAATATACAGAAGACAGGTTTTCAAGGACTCATTAATAAGACA; AACTACTGGTGAAAAAAAGAACCCTTTCCGATATTTTAGTAAACAAGAATTAAGAGAGCTCTTTACAATC; GAGGATCTTCAGAACTCTGTAACCCAGCTGCAGCTTCAGTCTTTGCATGCTGCTCAGAGGAAATCTGATA; TAAAACTAGATGAACATATTGCCTACCTGCAGTCTTTGGGGATAGCTGGAATCTCAGACCATGATTTGAT; GTACACATGTGATCTGTCTGTTAAAGAAGAGCTTGATGTGGTAGAAGAATCTCACTATATTCAACAAAGG; GTTCAGAAAGCTCAATTCCTCGTTGAATTCGAGTCTCAAAATAAAGAGTTCCTGATGGAACAACAAAGAA; CTAGAAATGAGGGGGCCTGGCTAAGAGAACCTGTATTTCCTTCTTCAACAAAGAAGAAATGCCCTAAATT; GAATAAACCACAGCCTCAGCCTTCACCTCTTCTAAGTACTCATCATACTCAGGAAGAAGATATCAGTTCC; AAAATGGCAAGTGTAGTCATTGATGATCTGCCCAAAGAGGGTGAGAAACAAGATCTCTCCAGTATAAAGG; TGAATGTTACCACCTTGCAAGATGGTAAAGGTACAGGTAGTGCTGACTCTATAGCTACTTTACCAAAGGG; GTTTGGAAGTGTAGAAGAACTTTGTACTAACTCTTCATTGGGAATGGAAAAAAGCTTTGCAACTAAAAAT; GAAGCTGTACAAAAAGAGACATTACAAGAGGGGCCTAAGCAAGAGGCACTGCAAGAGGATCCTCTGGAAA; GTTTTAATTATGTACTTAGCAAATCAACCAAAGCTGATATTGGGCCAAATTTAGATCAACTAAAGGATGA; TGAGATTTTACGTCATTGCAATCCTTGGCCCATTATTTCCATAACAAATGAAAGTCAAAATGCAGAATCA; AATGTATCCATTATTGAAATAGCTGATGACCTTTCAGCATCCCATAGTGCACTGCAGGATGCTCAAGCAA; GTGAGGCCAAGTTGGAAGAGGAACCTTCAGCATCTTCACCACAGTATGCATGTGATTTCAATCTTTTCTT; GGAAGACTCAGCAGACAACAGACAAAATTTTTCCAGTCAGTCTTTAGAGCATGTTGAGAAAGAAAATAGC; TTGTGTGGCTCTGCACCTAATTCCAGAGCAGGGTTTGTGCATAGCAAAACATGTCTCAGTTGGGAGTTTT; CTGAGAAAGACGATGAACCAGAAGAAGTAGTAGTTAAAGCAAAAATCAGAAGTAAAGCTAGAAGGATTGT; TTCAGATGGCGAAGATGAAGATGATTCTTTTAAAGATACCTCAAGCATAAATCCATTCAACACATCTCTC; TTTCAATTCTCATCTGTGAAACAATTTGATGCTTCAACTCCCAAAAATGACATCAGTCCACCAGGAAGGT; TCTTTTCATCTCAAATACCCAGTAGTGTAAATAAGTCTATGAACTCTAGAAGATCTCTGGCTTCTAGGAG; GTCTCTTATTAATATGGTTTTAGACCACGTGGAGGACATGGAGGAAAGACTTGACGACAGCAGTGAAGCA; AAGGGTCCTGAAGATTATCCAGAAGAAGGGGTGGAGGAAAGCAGTGGCGAAGCCTCCAAGTATACAGAAG; AGGATCCTTCCGGAGAAACACTGTCTTCAGAAAACAAGTCCAGCTGGTTAATGACGTCTAAGCCTAGTGC; TCTAGCTCAAGAGACCTCTCTTGGTGCCCCTGAGCCTTTGTCTGGTGAACAGTTGGTTGGTTCTCCCCAG; GATAAGGCGGCAGAGGCTACAAATGACTATGAGACTCTTGTAAAGCGTGGAAAAGAACTAAAAGAGTGTG; GAAAAATCCAGGAGGCCCTAAACTGCTTAGTTAAAGCGCTTGACATAAAAAGTGCAGATCCTGAAGTTAT; GCTCTTGACTTTAAGTTTGTATAAGCAACTTAATAACAAT; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC223966 representing NM_017669; Red=Cloning site Green=Tags(s); ; MEASRRFPEAEALSPEQAAHYLRYVKEAKEATKNGDLEEAFKLFNLAKDIFPNEKVLSRIQKIQEALEEL; AEQGDDEFTDVCNSGLLLYRELHNQLFEHQKEGIAFLYSLYRDGRKGGILADDMGLGKTVQIIAFLSGMF; DASLVNHVLLIMPTNLINTWVKEFIKWTPGMRVKTFHGPSKDERTRNLNRIQQRNGVIITTYQMLINNWQ; QLSSFRGQEFVWDYVILDEAHKIKTSSTKSAICARAIPASNRLLLTGTPIQNNLQELWSLFDFACQGSLL; GTLKTFKMEYENPITRAREKDATPGEKALGFKISENLMAIIKPYFLRRTKEDVQKKKSSNPEARLNEKNP; DVDAICEMPSLSRKNDLIIWIRLVPLQEEIYRKFVSLDHIKELLMETRSPLAELGVLKKLCDHPRLLSAR; ACCLLNLGTFSAQDGNEGEDSPDVDHIDQVTDDTLMEESGKMIFLMDLLKRLRDEGHQTLVFSQSRQILN; IIERLLKNRHFKTLRIDGTVTHLLEREKRINLFQQNKDYSVFLLTTQVGGVGLTLTAATRVVIFDPSWNP; ATDAQAVDRVYRIGQKENVVVYRLITCGTVEEKIYRRQVFKDSLIRQTTGEKKNPFRYFSKQELRELFTI; EDLQNSVTQLQLQSLHAAQRKSDIKLDEHIAYLQSLGIAGISDHDLMYTCDLSVKEELDVVEESHYIQQR; VQKAQFLVEFESQNKEFLMEQQRTRNEGAWLREPVFPSSTKKKCPKLNKPQPQPSPLLSTHHTQEEDISS; KMASVVIDDLPKEGEKQDLSSIKVNVTTLQDGKGTGSADSIATLPKGFGSVEELCTNSSLGMEKSFATKN; EAVQKETLQEGPKQEALQEDPLESFNYVLSKSTKADIGPNLDQLKDDEILRHCNPWPIISITNESQNAES; NVSIIEIADDLSASHSALQDAQASEAKLEEEPSASSPQYACDFNLFLEDSADNRQNFSSQSLEHVEKENS; LCGSAPNSRAGFVHSKTCLSWEFSEKDDEPEEVVVKAKIRSKARRIVSDGEDEDDSFKDTSSINPFNTSL; FQFSSVKQFDASTPKNDISPPGRFFSSQIPSSVNKSMNSRRSLASRRSLINMVLDHVEDMEERLDDSSEA; KGPEDYPEEGVEESSGEASKYTEEDPSGETLSSENKSSWLMTSKPSALAQETSLGAPEPLSGEQLVGSPQ; DKAAEATNDYETLVKRGKELKECGKIQEALNCLVKALDIKSADPEVMLLTLSLYKQLNNN; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Chromatograms: Chromatograms ; Sequencher program is needed, download here
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_017669
• ORF Size: 3750 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_017669.4
• RefSeq Size: 4224 bp
• RefSeq ORF: 3753 bp
• Locus ID: 54821
• UniProt ID: Q2NKX8
• Cytogenetics: Xq13.1
• MW: 141.1 kDa
• Summary: This gene encodes a member of the SWItch/Sucrose Non-Fermentable (SWI/SNF2) family of proteins, and contains a SNF2-like ATPase domain and a PICH family domain. One distinguishing feature of this SWI/SNF protein family member is that during interphase, the protein is excluded from the nucleus, and only associates with chromatin after the nuclear envelope has broken down. This protein is a DNA translocase that is thought to bind double-stranded DNA that is exposed to stretching forces, such as those exerted by the mitotic spindle. This protein associates with ribosomal DNA and ultra-fine DNA bridges (UFBs), fine structures that connect sister chromatids during anaphase at some sites such as fragile sites, telomeres and centromeres. This gene is required for the faithful segregation of sister chromatids during mitosis, and the ATPase activity of this protein required for the resolution of UFBs before cytokinesis. [provided by RefSeq, May 2017]