DHX36 (NM_020865) Human Tagged ORF Clone

SKU RC223929

DHX36 (Myc-DDK-tagged)-Human DEAH (Asp-Glu-Ala-His) box polypeptide 36 (DHX36), transcript variant 1

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: DHX36
• Synonyms: DDX36; G4R1; MLEL1; RHAU
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC223929 representing NM_020865; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGAGTTATGACTACCATCAGAACTGGGGCCGTGATGGGGGTCCCCGCAGCTCCGGTGGGGGCTATGGAG; GGGGGCCAGCAGGGGGTCATGGAGGTAACCGAGGCTCCGGAGGAGGCGGCGGCGGCGGAGGGGGTGGTCG; AGGCGGCAGGGGCCGGCATCCCGGGCACCTGAAAGGCCGCGAAATCGGCATGTGGTACGCGAAAAAACAG; GGGCAGAAGAACAAGGAAGCGGAGAGGCAAGAGAGAGCTGTAGTACACATGGATGAACGACGAGAAGAAC; AAATTGTACAGTTACTGAATTCTGTTCAAGCGAAGAATGATAAAGAGTCAGAAGCACAGATATCCTGGTT; TGCTCCTGAGGATCATGGATACGGTACTGAAGTTTCTACTAAGAACACACCATGCTCAGAGAACAAACTT; GACATCCAGGAAAAGAAGTTGATAAATCAAGAAAAAAAAATGTTTAGAATCAGGAACAGATCATATATTG; ACCGAGATTCTGAGTATCTCTTGCAAGAAAATGAACCAGATGGAACTTTAGACCAAAAATTATTGGAAGA; TTTACAAAAGAAAAAAAATGACCTTCGGTATATTGAAATGCAGCATTTCAGAGAAAAGCTGCCTTCGTAT; GGAATGCAAAAGGAATTGGTAAATTTAATTGATAACCATCAGGTAACAGTAATAAGTGGTGAAACTGGTT; GTGGCAAAACCACTCAAGTTACTCAGTTCATTTTGGATAACTACATTGAAAGAGGAAAAGGATCTGCTTG; CAGAATAGTTTGTACTCAGCCAAGAAGAATTAGTGCCATTTCAGTTGCGGAAAGAGTAGCTGCAGAAAGG; GCAGAATCTTGTGGCAGTGGTAATAGTACTGGATATCAAATTCGTCTCCAGAGTCGGTTGCCAAGGAAAC; AGGGTTCTATCTTATACTGTACAACAGGAATCATCCTTCAGTGGCTCCAGTCAGACCCGTATTTGTCCAG; TGTTAGTCATATCGTACTTGATGAAATCCATGAAAGAAATCTGCAGTCAGATGTTTTAATGACTGTTGTT; AAAGACCTTCTCAATTTTCGATCTGACTTGAAAGTAATATTGATGAGTGCAACATTGAATGCAGAAAAGT; TTTCAGAATATTTTGGTAACTGTCCAATGATACATATACCTGGTTTTACCTTTCCGGTTGTGGAATATCT; TTTGGAAGATGTAATTGAAAAAATAAGGTATGTTCCAGAACAAAAAGAACACAGATGCCAGTTTAAGAGG; GGTTTCATGCAAGGGCATGTAAATAGACAAGAAAAAGAAGAAAAAGAAGCAATATATAAAGAACGTTGGC; CAGATTATGTAAGGGAACTGCGAAGAAGGTATTCTGCAAGTACTGTAGATGTTATAGAAATGATGGAGGA; TGATAAAGTTGATCTGAATTTGATTGTTGCCCTCATCCGATACATTGTTTTGGAAGAAGAGGATGGTGCG; ATACTGGTCTTTCTGCCAGGCTGGGACAATATCAGCACTTTACATGATCTCTTGATGTCACAAGTAATGT; TTAAATCAGATAAATTTTTAATTATACCTTTACATTCACTGATGCCTACAGTTAACCAGACACAGGTGTT; TAAAAGAACCCCTCCTGGTGTTCGGAAAATAGTAATTGCTACCAACATTGCGGAGACTAGCATTACCATA; GATGATGTCGTTTATGTGATAGATGGAGGAAAAATAAAAGAGACGCATTTTGATACTCAGAACAATATCA; GTACAATGTCCGCTGAGTGGGTTAGTAAAGCTAATGCCAAACAGAGAAAAGGTCGAGCTGGAAGAGTTCA; ACCTGGTCATTGCTATCATCTGTATAATGGTCTTAGAGCAAGTCTTCTAGATGACTATCAACTGCCAGAA; ATTTTGAGAACTCCTTTGGAAGAACTTTGTTTACAAATAAAGATTTTAAGGCTAGGTGGAATTGCTTATT; TTCTGAGTAGATTAATGGACCCACCATCAAATGAGGCAGTGTTACTCTCCATAAGACACCTGATGGAGCT; GAACGCTTTGGATAAACAAGAAGAATTGACACCTCTTGGAGTCCACTTGGCACGATTACCCGTTGAGCCA; CATATTGGAAAAATGATTCTTTTTGGAGCACTGTTCTGCTGCTTAGACCCAGTACTCACTATTGCTGCTA; GTCTCAGTTTCAAAGATCCATTTGTCATTCCACTGGGAAAAGAAAAGATTGCAGATGCAAGAAGAAAGGA; ATTGGCAAAGGATACTAGAAGTGATCACTTAACAGTTGTGAATGCGTTTGAGGGCTGGGAAGAGGCTAGG; CGACGTGGTTTCAGATACGAAAAGGACTATTGCTGGGAATATTTTCTGTCTTCAAACACACTGCAGATGC; TGCATAACATGAAAGGACAGTTTGCTGAGCATCTTCTTGGAGCTGGATTTGTAAGCAGTAGAAATCCTAA; AGATCCAGAATCTAATATAAATTCAGATAATGAGAAGATAATTAAAGCTGTCATCTGTGCTGGTTTATAT; CCCAAAGTTGCTAAAATTCGACTAAATTTGGGTAAAAAAAGAAAAATGGTAAAAGTTTACACAAAAACCG; ATGGCCTGGTTGCTGTTCATCCTAAATCTGTTAATGTGGAGCAAACAGACTTTCACTACAACTGGCTTAT; CTATCACCTAAAGATGAGAACAAGCAGTATATACTTGTATGACTGCACAGAGGTTTCCCCATACTGTCTC; TTGTTTTTTGGAGGTGACATTTCCATCCAGAAGGATAACGATCAGGAAACTATTGCTGTAGATGAGTGGA; TTGTATTTCAGTCTCCAGCAAGAATTGCCCATCTTGTTAAGGAATTAAGAAAGGAACTAGATATTCTTCT; GCAAGAGAAGATTGAAAGTCCTCATCCTGTAGACTGGAATGACACTAAATCCAGAGACTGTGCAGTACTG; TCAGCTATTATAGACTTGATCAAAACACAGGAAAAGGCAACTCCCAGGAACTTTCCGCCACGATTCCAGG; ATGGATATTACAGC; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC223929 representing NM_020865; Red=Cloning site Green=Tags(s); ; MSYDYHQNWGRDGGPRSSGGGYGGGPAGGHGGNRGSGGGGGGGGGGRGGRGRHPGHLKGREIGMWYAKKQ; GQKNKEAERQERAVVHMDERREEQIVQLLNSVQAKNDKESEAQISWFAPEDHGYGTEVSTKNTPCSENKL; DIQEKKLINQEKKMFRIRNRSYIDRDSEYLLQENEPDGTLDQKLLEDLQKKKNDLRYIEMQHFREKLPSY; GMQKELVNLIDNHQVTVISGETGCGKTTQVTQFILDNYIERGKGSACRIVCTQPRRISAISVAERVAAER; AESCGSGNSTGYQIRLQSRLPRKQGSILYCTTGIILQWLQSDPYLSSVSHIVLDEIHERNLQSDVLMTVV; KDLLNFRSDLKVILMSATLNAEKFSEYFGNCPMIHIPGFTFPVVEYLLEDVIEKIRYVPEQKEHRCQFKR; GFMQGHVNRQEKEEKEAIYKERWPDYVRELRRRYSASTVDVIEMMEDDKVDLNLIVALIRYIVLEEEDGA; ILVFLPGWDNISTLHDLLMSQVMFKSDKFLIIPLHSLMPTVNQTQVFKRTPPGVRKIVIATNIAETSITI; DDVVYVIDGGKIKETHFDTQNNISTMSAEWVSKANAKQRKGRAGRVQPGHCYHLYNGLRASLLDDYQLPE; ILRTPLEELCLQIKILRLGGIAYFLSRLMDPPSNEAVLLSIRHLMELNALDKQEELTPLGVHLARLPVEP; HIGKMILFGALFCCLDPVLTIAASLSFKDPFVIPLGKEKIADARRKELAKDTRSDHLTVVNAFEGWEEAR; RRGFRYEKDYCWEYFLSSNTLQMLHNMKGQFAEHLLGAGFVSSRNPKDPESNINSDNEKIIKAVICAGLY; PKVAKIRLNLGKKRKMVKVYTKTDGLVAVHPKSVNVEQTDFHYNWLIYHLKMRTSSIYLYDCTEVSPYCL; LFFGGDISIQKDNDQETIAVDEWIVFQSPARIAHLVKELRKELDILLQEKIESPHPVDWNDTKSRDCAVL; SAIIDLIKTQEKATPRNFPPRFQDGYYS; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Chromatograms: Chromatograms ; Sequencher program is needed, download here
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_020865
• ORF Size: 3024 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_020865.1, NP_065916.1
• RefSeq Size: 3600 bp
• RefSeq ORF: 3027 bp
• Locus ID: 170506
• UniProt ID: Q9H2U1
• Cytogenetics: 3q25.2
• Domains: DEAD, HA2, helicase_C
• MW: 114.6 kDa
• Summary: This gene is a member of the DEAH-box family of RNA-dependent NTPases which are named after the conserved amino acid sequence Asp-Glu-Ala-His in motif II. The protein encoded by this gene has been shown to enhance the deadenylation and decay of mRNAs with 3'-UTR AU-rich elements (ARE-mRNA). The protein has also been shown to resolve into single strands the highly stable tetramolecular DNA configuration (G4) that can form spontaneously in guanine-rich regions of DNA. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]