P4HA1 (NM_000917) Human Tagged ORF Clone

SKU RC223831

P4HA1 (Myc-DDK-tagged)-Human prolyl 4-hydroxylase, alpha polypeptide I (P4HA1), transcript variant 1

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: P4HA1
• Synonyms: P4HA
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC223831 representing NM_000917; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGATCTGGTATATATTAATTATAGGAATTCTGCTTCCCCAGTCTTTGGCTCATCCAGGCTTTTTTACTT; CAATTGGTCAGATGACTGATTTGATCCATACTGAGAAAGATCTGGTGACTTCTCTGAAAGATTATATTAA; GGCAGAAGAGGACAAGTTAGAACAAATAAAAAAATGGGCAGAGAAGTTAGATCGGCTAACTAGTACAGCG; ACAAAAGATCCAGAAGGATTTGTTGGGCATCCAGTAAATGCATTCAAATTAATGAAACGTCTGAATACTG; AGTGGAGTGAGTTGGAGAATCTGGTCCTTAAGGATATGTCAGATGGCTTTATCTCTAACCTAACCATTCA; GAGACAGTACTTTCCTAATGATGAAGATCAGGTTGGGGCAGCCAAAGCTCTGTTACGTCTCCAGGATACC; TACAATTTGGATACAGATACCATCTCAAAGGGTAATCTTCCAGGAGTGAAACACAAATCTTTTCTAACGG; CTGAGGACTGCTTTGAGTTGGGCAAAGTGGCCTATACAGAAGCAGATTATTACCATACGGAACTGTGGAT; GGAACAAGCCCTAAGGCAACTGGATGAAGGCGAGATTTCTACCATAGATAAAGTCTCTGTTCTAGATTAT; TTGAGCTATGCGGTATATCAGCAGGGAGACCTGGATAAGGCACTTTTGCTCACAAAGAAGCTTCTTGAAC; TAGATCCTGAACATCAGAGAGCTAATGGTAACTTAAAATATTTTGAGTATATAATGGCTAAAGAAAAAGA; TGTCAATAAGTCTGCTTCAGATGACCAATCTGATCAGAAAACTACACCAAAGAAAAAAGGGGTTGCTGTG; GATTACCTGCCAGAGAGACAGAAGTACGAAATGCTGTGCCGTGGGGAGGGTATCAAAATGACCCCTCGGA; GACAGAAAAAACTCTTTTGCCGCTACCATGATGGAAACCGTAATCCTAAATTTATTCTGGCTCCAGCTAA; ACAGGAGGATGAATGGGACAAGCCTCGTATTATTCGCTTCCATGATATTATTTCTGATGCAGAAATTGAA; ATCGTCAAAGACCTAGCAAAACCAAGGCTGAGGCGAGCCACCATTTCAAACCCAATAACAGGAGACTTGG; AGACGGTACATTACAGAATTAGCAAAAGTGCCTGGCTCTCTGGCTATGAAAATCCTGTGGTGTCTCGAAT; TAATATGAGAATACAAGATCTAACAGGACTAGATGTTTCCACAGCAGAGGAATTACAGGTAGCAAATTAT; GGAGTTGGAGGACAGTATGAACCCCATTTTGACTTTGCACGGAAAGATGAGCCAGATGCTTTCAAAGAGC; TGGGGACAGGAAATAGAATTGCTACATGGCTGTTTTATATGAGTGATGTGTCTGCAGGAGGAGCCACTGT; TTTTCCTGAAGTTGGAGCTAGTGTTTGGCCCAAAAAAGGAACTGCTGTTTTCTGGTATAATCTGTTTGCC; AGTGGAGAAGGAGATTATAGTACACGGCATGCAGCCTGTCCAGTGCTAGTTGGCAACAAATGGGTATCCA; ATAAATGGCTCCATGAACGTGGACAAGAATTTCGAAGACCTTGTACGTTGTCAGAATTGGAA; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC223831 representing NM_000917; Red=Cloning site Green=Tags(s); ; MIWYILIIGILLPQSLAHPGFFTSIGQMTDLIHTEKDLVTSLKDYIKAEEDKLEQIKKWAEKLDRLTSTA; TKDPEGFVGHPVNAFKLMKRLNTEWSELENLVLKDMSDGFISNLTIQRQYFPNDEDQVGAAKALLRLQDT; YNLDTDTISKGNLPGVKHKSFLTAEDCFELGKVAYTEADYYHTELWMEQALRQLDEGEISTIDKVSVLDY; LSYAVYQQGDLDKALLLTKKLLELDPEHQRANGNLKYFEYIMAKEKDVNKSASDDQSDQKTTPKKKGVAV; DYLPERQKYEMLCRGEGIKMTPRRQKKLFCRYHDGNRNPKFILAPAKQEDEWDKPRIIRFHDIISDAEIE; IVKDLAKPRLRRATISNPITGDLETVHYRISKSAWLSGYENPVVSRINMRIQDLTGLDVSTAEELQVANY; GVGGQYEPHFDFARKDEPDAFKELGTGNRIATWLFYMSDVSAGGATVFPEVGASVWPKKGTAVFWYNLFA; SGEGDYSTRHAACPVLVGNKWVSNKWLHERGQEFRRPCTLSELE; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Chromatograms: Chromatograms ; Sequencher program is needed, download here
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_000917
• ORF Size: 1602 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq Size: 2752 bp
• RefSeq ORF: 1605 bp
• Locus ID: 5033
• UniProt ID: P13674
• Cytogenetics: 10q22.1
• Domains: 2OG-FeII_Oxy, P4Hc
• Protein Families: Druggable Genome, P450
• Protein Pathways: Arginine and proline metabolism, Metabolic pathways
• MW: 61.05 kDa
• Summary: This gene encodes a component of prolyl 4-hydroxylase, a key enzyme in collagen synthesis composed of two identical alpha subunits and two beta subunits. The encoded protein is one of several different types of alpha subunits and provides the major part of the catalytic site of the active enzyme. In collagen and related proteins, prolyl 4-hydroxylase catalyzes the formation of 4-hydroxyproline that is essential to the proper three-dimensional folding of newly synthesized procollagen chains. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008]