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SHANK1 (NM_016148) Human Tagged ORF Clone
CAT#: RC223485
- TrueORF®
SHANK1 (Myc-DDK-tagged)-Human SH3 and multiple ankyrin repeat domains 1 (SHANK1)
ORF Plasmid: tGFP
"NM_016148" in other vectors (2)
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Other products for "SHANK1"
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Symbol | SHANK1 |
| Synonyms | SPANK-1; SSTRIP; synamon |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC223485 representing NM_016148
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACCCACAGCCCCGCGACAAGCGAGGACGAGGAACGCCACAGTGCCAGCGAGTGTCCCGAGGGGGGCT CAGAGTCCGACAGCTCCCCAGACGGGCCAGGTCGAGGCCCCCGGGGGACCCGGGGCCAGGGCAGTGGGGC ACCTGGTAGCCTGGCCTCTGTTAGAGGCCTCCAGGGCCGCTCAATGTCCGTCCCAGACGACGCCCACTTC AGCATGATGGTCTTCAGGATTGGCATCCCGGACCTGCACCAGACAAAATGCCTTCGCTTCAACCCCGATG CCACCATCTGGACGGCCAAGCAGCAGGTGCTCTGTGCCCTGAGCGAGAGCCTGCAGGATGTGCTCAACTA TGGCCTGTTCCAACCGGCCACCTCCGGCCGCGATGCCAACTTCCTGGAGGAGGAGAGGCTGCTGCGGGAG TACCCCCAGTCCTTTGAGAAGGGGGTCCCCTACCTGGAGTTCCGATACAAGACCCGAGTTTACAAACAGA CCAACCTGGATGAGAAGCAGCTGGCCAAGTTGCACACGAAGACGGGGTTGAAGAAGTTCCTGGAGTATGT GCAGCTCGGGACATCTGACAAGGTGGCGCGGCTGCTGGACAAGGGGCTGGACCCCAATTACCATGACTCG GATTCGGGAGAGACCCCCTTGACACTGGCGGCCCAGACCGAAGGCTCTGTAGAGGTGATTCGAACCCTGT GCCTGGGCGGGGCCCACATTGACTTCCGGGCCCGGGATGGCATGACCGCACTGCATAAGGCCGCATGCGC CCGACACTGCCTGGCACTCACGGCGCTCCTGGACCTTGGGGGTTCCCCCAACTACAAGGACCGTCGGGGG CTGACCCCTCTGTTCCACACGGCCATGGTGGGTGGTGACCCCCGATGCTGCGAGCTGCTCCTGTTCAACA GGGCCCAGCTGGGCATAGCTGATGAGAACGGCTGGCAGGAAATCCACCAGGCCTGCCAGCGGGGTCACTC TCAGCACCTGGAGCATCTGCTTTTCTACGGGGCTGAGCCTGGAGCCCAGAACGCCTCGGGGAACACGGCT CTGCACATCTGCGCCCTCTACAACAAGGAGACCTGTGCCAGGATCCTCCTGTATCGAGGTGCCGACAAGG ATGTGAAGAACAACAACGGACAGACCCCCTTCCAGGTGGCAGTGATTGCTGGGAATTTTGAGCTGGGGGA GCTGATCCGAAACCACCGAGAACAGGATGTGGTGCCCTTCCAGGAGTCCCCCAAGTACGCGGCCCGGCGA CGGGGGCCCCCAGGCACAGGGCTGACGGTGCCCCCGGCGCTGCTGCGGGCCAACAGTGACACCAGCATGG CGCTGCCCGACTGGATGGTGTTCTCCGCCCCGGGGGCCGCGTCCTCTGGGGCCCCTGGCCCTACCTCAGG GTCCCAGGGCCAGTCGCAGCCCTCGGCCCCCACCACCAAGCTCAGCAGCGGGACCCTCCGAAGTGCCAGC AGCCCCCGGGGTGCCAGGGCCCGCTCTCCATCCCGAGGGAGGCACCCTGAGGACGCCAAGAGGCAGCCCC GAGGCCGGCCCAGCTCCAGCGGGACACCCCGGGAAGGGCCAGCCGGGGGCACGGGGGGCTCAGGGGGCCC CGGGGGCTCCCTGGGCAGCCGCGGGAGGCGGAGGAAGCTCTACTCAGCGGTACCCGGACGCTCCTTCATG GCTGTGAAGTCCTACCAGGCCCAAGCCGAGGGGGAGATCTCCCTGAGCAAGGGCGAGAAGATCAAAGTAC TTAGCATCGGGGAAGGAGGCTTCTGGGAAGGCCAGGTCAAAGGTCGTGTTGGCTGGTTCCCCTCTGACTG CCTGGAAGAAGTGGCGAATCGCTCTCAGGAGAGCAAGCAAGAAAGCCGCAGTGACAAGGCAAAGAGACTC TTCCGGCATTATACCGTGGGCTCCTACGACAGCTTTGATGCCCCAAGCTTAATGGATGGGATTGGCCCAG GGAGCGATTACATCATTAAGGAGAAGACAGTCTTGCTGCAGAAGAAGGACAGTGAGGGGTTTGGGTTCGT GCTCCGGGGGGCCAAGGCGCAGACCCCCATCGAGGAGTTCACCCCCACCCCGGCCTTCCCGGCGCTGCAG TACCTGGAGTCGGTGGACGAGGGTGGCGTGGCATGGCGAGCTGGACTGCGAATGGGAGACTTCCTCATCG AGGTGAACGGGCAGAATGTGGTGAAGGTCGGCCACCGACAGGTGGTGAACATGATCCGCCAAGGGGGCAA CACGCTGATGGTGAAGGTGGTGATGGTCACCAGGCACCCGGACATGGATGAGGCAGTGCACAAGAAAGCA CCCCAGCAGGCCAAGCGGCTGCCGCCCCCAACCATCTCCCTGCGTTCCAAATCTATGACCTCAGAGCTGG AGGAGATGGAGTACGAGCAGCAGCCGGCGCCGGTGCCCAGCATGGAGAAAAAGCGGACCGTGTATCAGAT GGCTCTCAACAAACTGGACGAAATCCTGGCCGCAGCTCAACAGACCATCAGTGCAAGCGAAAGCCCTGGT CCCGGTGGCCTCGCGTCCCTGGGCAAACACCGACCCAAAGGTTTCTTTGCCACTGAGTCGAGCTTCGATC CCCACCACCGTGCCCAGCCAAGTTACGAGCGTCCTTCTTTCCTGCCTCCAGGACCTGGGTTGATGCTCCG GCAAAAATCTATCGGTGCGGCAGAAGATGACAGACCTTACCTAGCACCCCCAGCCATGAAATTCAGCCGC AGCCTGTCTGTGCCTGGTTCGGAGGACATTCCCCCGCCACCCACCACGTACCCACCGGAGCCTCCCTACA GCACACCTACAGTCCCCTCCTCCTCAGGGCGCCTCACCCCCTCCCCTCGGGGAGGGCCCTTCAACCCTGG CTCTGGTGGCCCCCTCCCCGCCTCCTCCCCTGCAACCTTTGACGGGCCCTCCCCTCCCGACACTCGCGTG GGGAGCCGCGAGAAGAGCCTGTACCACAGTGGGCCCCTGCCCCCGGCCCACCACCACCCGCCCCACCACC ACCACCACCACGCCCCGCCCCCTCAGCCCCACCACCACCACGCCCACCCCCCTCATCCTCCCGAGATGGA GACAGGCGGCTCTCCCGACGACCCTCCACCCCGCCTGGCTCTGGGGCCCCAGCCCAGCCTGCGAGGCTGG AGGGGCGGCGGGCCCAGCCCGACCCCGGGGGCCCCGTCCCCATCGCACCACGGCAGCGCGGGCGGGGGCG GCGGCTCCTCCCAGGGCCCGGCTCTACGCTATATCCAGCTGCCCCCGCGGGCGGCCAGCGCAGCCATGTA CGTGCCCGCCCGCTCGGGCCGCGGCCGCAAGGGCCCGCTGGTCAAGCAGACCAAGGTGGAAGGCGAGCCC CAGAAGGGCGGCGGCCTCCCGCCCGCGCCGTCGCCCTCGTCCCCGGCCTCCCCGCAGCCGCCGCCCGCCG TGGCCGCGCCCTCGGAGAAGAACTCCATCCCCATCCCCACCATCATCATCAAGGCCCCGTCCACCAGTAG CAGCGGCCGCAGCAGCCAGGGCAGCAGCACCGAGGCGGAGCCCCCCACCCAGCCGGAGCCCACGGGAGGC GGCGGCGGCGGCGGCTCCTCGCCCAGCCCCGCCCCGGCCATGTCACCCGTGCCCCCGTCCCCCTCGCCCG TGCCCACCCCCGCCTCGCCCAGCGGCCCGGCCACGCTGGACTTCACGAGCCAGTTCGGGGCCGCCCTGGT GGGGGCGGCCCGGAGGGAGGGGGGCTGGCAGAATGAGGCGCGCCGGCGCTCCACGCTGTTCCTGTCCACC GACGCGGGGGACGAGGACGGCGGGGACGGCGGGCTGGGCACAGGGGCGGCCCCGGGCCCGCGGCTGCGCC ACTCCAAATCCATCGACGAGGGCATGTTCTCCGCCGAGCCCTACCTCCGACTGGAGTCTGCGGGCAGCGG CGCGGGCTACGGCGGCTACGGGGCCGGTAGCCGAGCCTACGGGGGTGGCGGGGGCAGCAGCGCCTTCACC AGCTTCCTGCCCCCGCGACCCCTGGTGCACCCGCTGACCGGCAAGGCCCTGGATCCCGCCTCCCCGCTGG GGCTGGCCCTGGCCGCCCGCGAGCGAGCGCTGAAGGAGTCCTCGGAGGGCGGCGGGGCCCCCCAGCCGCC TCCCAGGCCCCCATCGCCCCGCTACGAGGCCCCGCCGCCCACCCCGCACCACCACTCGCCCCACGCCCAC CACGAGCCAGTGCTGCGTCTCTGGGGGGCCTCCCCGCCGGACCCTGCGCGCCGGGAGCTGGGGTACAGGG CCGGGCTGGGCAGCCAGGAGAAGTCCCTTCCCGCCAGCCCGCCCGCCGCCCGGCGTTCCCTGCTACACCG CCTGCCGCCCACCGCTCCCGGGGTGGGGCCCCTCCTGCTGCAGCTGGGGACGGAGCCCCCGGCCCCGCAC CCCGGAGTAAGCAAGCCCTGGAGGTCCGCAGCCCCCGAAGAACCCGAGCGGCTGCCGCTGCACGTGCGGT TCCTTGAAAACTGCCAGCCCCGGGCCCCTGTGACGAGCGGAAGGGGTCCCCCCTCGGAGGACGGGCCGGG GGTCCCGCCGCCCAGCCCACGCCGGTCCGTGCCCCCCTCCCCGACCTCCCCGAGGGCCAGCGAAGAGAAC GGGCTGCCCCTGCTGGTCCTGCCGCCTCCCGCCCCCTCGGTGGATGTGGAAGATGGCGAATTCCTTTTCG TGGAACCGCTGCCTCCGCCTCTGGAATTCTCCAACAGCTTCGAAAAGCCAGAGTCGCCCCTCACGCCTGG GCCTCCCCACCCGCTGCCCGACACACCTGCCCCTGCCACCCCGTTACCCCCTGTGCCACCCCCGGCTGTG GCCGCAGCCCCTCCCACCCTGGACTCCACCGCATCCAGCCTGACATCCTATGACAGCGAGGTGGCCACCC TGACCCAGGGGGCCTCCGCCGCTCCTGGGGACCCCCATCCACCAGGCCCGCCTGCCCCAGCAGCACCGGC TCCCGCTGCCCCACAGCCTGGCCCGGACCCTCCGCCTGGCACGGATTCTGGCATCGAGGAGGTGGACAGT CGGAGCAGCAGTGACCACCCACTGGAGACCATCAGCAGCGCCTCCACGCTGAGCAGCCTATCTGCCGAAG GTGGTGGCAGCGCAGGGGGTGGGGGCGGGGCTGGGGCCGGTGTGGCCAGTGGGCCGGAGCTTCTGGACAC CTATGTGGCCTACCTGGACGGCCAGGCCTTTGGGGGCAGCAGTACTCCCGGCCCGCCATACCCTCCTCAG CTCATGACTCCCTCTAAGCTCCGGGGCCGGGCGCTAGGAGCCAGCGGAGGCCTGCGGCCTGGCCCCAGCG GGGGACTCCGAGACCCTGTTACCCCCACCAGCCCCACCGTCTCGGTGACAGGGGCTGGAACCGATGGGCT GCTGGCCCTGCGTGCTTGTTCAGGACCCCCCACGGCAGGCGTGGCGGGGGGTCCGGTGGCTGTAGAGCCA GAAGTCCCACCGGTGCCCTTGCCGACGGCCTCCTGTCTGCCCCGGAAGCTGCTGCCCTGGGAGGAGGGCC CGGGCCCACCGCCACCACCTCTGCCCGGGCCCTTGGCCCAGCCTCAGGCCTCAGCCTTGGCCACAGTAAA AGCCAGCATCATCAGTGAACTCAGCTCCAAGCTTCAGCAGTTTGGGGGCTCCTCGGCAGCTGGCGGCGCT CTGCCCTGGGCCCGAGGAGGCAGTGGGGGAGGCGGAGACAGCCACCACGGGGGAGCCAGCTATGTCCCCG AGAGGACCTCCTCCCTGCAGCGGCAGAGACTCTCCGACGACTCCCAGTCCTCACTCCTCTCCAAGCCTGT CAGCAGCCTGTTTCAGAACTGGCCCAAACCACCTCTGCCGCCACTCCCCACCGGAACAGGGGTCTCCCCT ACAGCCGCTGCGGCCCCAGGGGCCACCTCACCCTCAGCCTCCTCCTCCTCCACGTCCACCCGCCACCTCC AGGGCGTGGAGTTCGAGATGCGGCCCCCTCTGCTCCGCCGGGCCCCCAGCCCCTCGCTGCTGCCCGCCTC GGAGCACAAGGTCAGCCCTGCGCCCAGGCCCTCGTCCCTGCCCATCCTGCCTTCCGGACCCCTCTACCCA GGCCTCTTTGACATCCGTGGCTCCCCAACTGGAGGGGCAGGAGGCTCGGCTGACCCCTTCGCCCCAGTCT TTGTGCCGCCACACCCGGGGATATCCGGGGGGCTCGGGGGAGCCTTGTCAGGGGCCTCGCGCTCCCTCTC ACCGACCCGCCTGCTCTCGCTGCCCCCGGACAAGCCGTTTGGCGCTAAACCTCTGGGGTTCTGGACCAAG TTCGACGTGGCTGATTGGCTGGAGTGGCTGGGTTTGGCGGAGCACCGAGCCCAGTTCCTGGACCACGAGA TCGATGGCTCCCACCTGCCCGCCTTGACCAAGGAGGACTACGTCGATCTAGGTGTGACCAGGGTGGGCCA CCGCATGAACATCGACCGGGCTCTCAAATTCTTCCTGGAGAGG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC223485 representing NM_016148
Red=Cloning site Green=Tags(s) MTHSPATSEDEERHSASECPEGGSESDSSPDGPGRGPRGTRGQGSGAPGSLASVRGLQGRSMSVPDDAHF SMMVFRIGIPDLHQTKCLRFNPDATIWTAKQQVLCALSESLQDVLNYGLFQPATSGRDANFLEEERLLRE YPQSFEKGVPYLEFRYKTRVYKQTNLDEKQLAKLHTKTGLKKFLEYVQLGTSDKVARLLDKGLDPNYHDS DSGETPLTLAAQTEGSVEVIRTLCLGGAHIDFRARDGMTALHKAACARHCLALTALLDLGGSPNYKDRRG LTPLFHTAMVGGDPRCCELLLFNRAQLGIADENGWQEIHQACQRGHSQHLEHLLFYGAEPGAQNASGNTA LHICALYNKETCARILLYRGADKDVKNNNGQTPFQVAVIAGNFELGELIRNHREQDVVPFQESPKYAARR RGPPGTGLTVPPALLRANSDTSMALPDWMVFSAPGAASSGAPGPTSGSQGQSQPSAPTTKLSSGTLRSAS SPRGARARSPSRGRHPEDAKRQPRGRPSSSGTPREGPAGGTGGSGGPGGSLGSRGRRRKLYSAVPGRSFM AVKSYQAQAEGEISLSKGEKIKVLSIGEGGFWEGQVKGRVGWFPSDCLEEVANRSQESKQESRSDKAKRL FRHYTVGSYDSFDAPSLMDGIGPGSDYIIKEKTVLLQKKDSEGFGFVLRGAKAQTPIEEFTPTPAFPALQ YLESVDEGGVAWRAGLRMGDFLIEVNGQNVVKVGHRQVVNMIRQGGNTLMVKVVMVTRHPDMDEAVHKKA PQQAKRLPPPTISLRSKSMTSELEEMEYEQQPAPVPSMEKKRTVYQMALNKLDEILAAAQQTISASESPG PGGLASLGKHRPKGFFATESSFDPHHRAQPSYERPSFLPPGPGLMLRQKSIGAAEDDRPYLAPPAMKFSR SLSVPGSEDIPPPPTTYPPEPPYSTPTVPSSSGRLTPSPRGGPFNPGSGGPLPASSPATFDGPSPPDTRV GSREKSLYHSGPLPPAHHHPPHHHHHHAPPPQPHHHHAHPPHPPEMETGGSPDDPPPRLALGPQPSLRGW RGGGPSPTPGAPSPSHHGSAGGGGGSSQGPALRYIQLPPRAASAAMYVPARSGRGRKGPLVKQTKVEGEP QKGGGLPPAPSPSSPASPQPPPAVAAPSEKNSIPIPTIIIKAPSTSSSGRSSQGSSTEAEPPTQPEPTGG GGGGGSSPSPAPAMSPVPPSPSPVPTPASPSGPATLDFTSQFGAALVGAARREGGWQNEARRRSTLFLST DAGDEDGGDGGLGTGAAPGPRLRHSKSIDEGMFSAEPYLRLESAGSGAGYGGYGAGSRAYGGGGGSSAFT SFLPPRPLVHPLTGKALDPASPLGLALAARERALKESSEGGGAPQPPPRPPSPRYEAPPPTPHHHSPHAH HEPVLRLWGASPPDPARRELGYRAGLGSQEKSLPASPPAARRSLLHRLPPTAPGVGPLLLQLGTEPPAPH PGVSKPWRSAAPEEPERLPLHVRFLENCQPRAPVTSGRGPPSEDGPGVPPPSPRRSVPPSPTSPRASEEN GLPLLVLPPPAPSVDVEDGEFLFVEPLPPPLEFSNSFEKPESPLTPGPPHPLPDTPAPATPLPPVPPPAV AAAPPTLDSTASSLTSYDSEVATLTQGASAAPGDPHPPGPPAPAAPAPAAPQPGPDPPPGTDSGIEEVDS RSSSDHPLETISSASTLSSLSAEGGGSAGGGGGAGAGVASGPELLDTYVAYLDGQAFGGSSTPGPPYPPQ LMTPSKLRGRALGASGGLRPGPSGGLRDPVTPTSPTVSVTGAGTDGLLALRACSGPPTAGVAGGPVAVEP EVPPVPLPTASCLPRKLLPWEEGPGPPPPPLPGPLAQPQASALATVKASIISELSSKLQQFGGSSAAGGA LPWARGGSGGGGDSHHGGASYVPERTSSLQRQRLSDDSQSSLLSKPVSSLFQNWPKPPLPPLPTGTGVSP TAAAAPGATSPSASSSSTSTRHLQGVEFEMRPPLLRRAPSPSLLPASEHKVSPAPRPSSLPILPSGPLYP GLFDIRGSPTGGAGGSADPFAPVFVPPHPGISGGLGGALSGASRSLSPTRLLSLPPDKPFGAKPLGFWTK FDVADWLEWLGLAEHRAQFLDHEIDGSHLPALTKEDYVDLGVTRVGHRMNIDRALKFFLER TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_016148 |
| ORF Size | 6483 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_016148.1, NP_057232.1 |
| RefSeq Size | 6643 bp |
| RefSeq ORF | 6486 bp |
| Locus ID | 50944 |
| UniProt ID | Q9Y566 |
| Cytogenetics | 19q13.33 |
| MW | 224.8 kDa |
| Gene Summary | This gene encodes a member of the SHANK (SH3 domain and ankyrin repeat containing) family of proteins. Members of this family act as scaffold proteins that are required for the development and function of neuronal synapses. Deletions in this gene may be associated with autism spectrum disorder in males. [provided by RefSeq, Apr 2016] |
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