RNF14 (NM_183401) Human Tagged ORF Clone

SKU RC223420

RNF14 (Myc-DDK-tagged)-Human ring finger protein 14 (RNF14), transcript variant 5

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: RNF14
• Synonyms: ARA54; HFB30; HRIHFB2038; TRIAD2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC223420 representing NM_183401.; Blue=ORF Red=Cloning site Green=Tag(s); ; GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG; GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC; ATGTCGTCAGAAGATCGAGAAGCTCAGGAGGATGAATTGCTGGCCCTGGCAAGTATTTACGATGGAGAT; GAATTTAGAAAAGCAGAGTCTGTCCAAGGTGGAGAAACCAGGATCTATTTGGATTTGCCACAGAATTTC; AAGATATTTGTGAGCGGCAATTCAAATGAGTGTCTCCAGAATAGTGGCTTTGAATACACCATTTGCTTT; CTGCCTCCACTTGTGCTGAACTTTGAACTGCCACCAGATTATCCATCCTCTTCCCCACCTTCATTCACA; CTTAGTGGCAAATGGCTGTCACCAACTCAGCTATCTGCTCTATGCAAGCACTTAGACAACCTATGGGAA; GAACACCGTGGCAGCGTGGTCCTGTTTGCCTGGATGCAATTTCTTAAGGAAGAGACCCTAGCATACTTG; AATATTGTCTCTCCTTTTGAGCTCAAGATTGGTTCTCAGAAAAAAGTGCAGAGAAGGACAGCTCAAGCT; TCTCCCAACACAGAGCTAGATTTTGGAGGAGCTGCTGGATCTGATGTAGACCAAGAGGAAATTGTGGAT; GAGAGAGCAGTGCAGGATGTGGAATCACTGTCAAATCTGATCCAGGAAATCTTGGACTTTGATCAAGCT; CAGCAGATAAAATGCTTTAATAGTAAATTGTTCCTGTGCAGTATCTGTTTCTGTGAGAAGCTGGGTAGT; GAATGCATGTACTTCTTGGAGTGCAGGCATGTGTACTGCAAAGCCTGTCTGAAGGACTACTTTGAAATC; CAGATCAGAGATGGCCAGGTTCAATGCCTCAACTGCCCAGAACCAAAGTGCCCTTCGGTGGCCACTCCT; GGTCAGGTCAAAGAGTTAGTGGAAGCAGAGTTATTTGCCCGTTATGACCGCCTTCTCCTCCAGTCCTCC; TTGGACCTGATGGCAGATGTGGTGTACTGCCCCCGGCCGTGCTGCCAGCTGCCTGTGATGCAGGAACCT; GGCTGCACCATGGGTATCTGCTCCAGCTGCAATTTTGCCTTCTGTACTTTGTGCAGGTTGACCTACCAT; GGGGTCTCCCCATGTAAGGTGACTGCAGAGAAATTAATGGACTTACGAAATGAATACCTGCAAGCGGAT; GAGGCTAATAAAAGACTTTTGGATCAAAGGTATGGTAAGAGAGTGATTCAGAAGGCACTGGAAGAGATG; GAAAGTAAGGAGTGGCTAGAGAAGAACTCAAAGAGCTGCCCATGTTGTGGAACTCCCATAGAGAAATTA; GACGGATGTAACAAGATGACATGTACTGGCTGTATGCAATATTTCTGTTGGATTTGCATGGGTTCTCTC; TCTAGAGCAAACCCTTACAAACATTTCAATGACCCTGGTTCACCATGTTTTAACCGGCTGTTTTATGCT; GTGGATGTTGACGACGATATTTGGGAAGATGAGGTAGAAGAC; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT; TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC;
  Protein Sequence

  >Peptide sequence encoded by RC223420; Blue=ORF Red=Cloning site Green=Tag(s); ; MSSEDREAQEDELLALASIYDGDEFRKAESVQGGETRIYLDLPQNFKIFVSGNSNECLQNSGFEYTICF; LPPLVLNFELPPDYPSSSPPSFTLSGKWLSPTQLSALCKHLDNLWEEHRGSVVLFAWMQFLKEETLAYL; NIVSPFELKIGSQKKVQRRTAQASPNTELDFGGAAGSDVDQEEIVDERAVQDVESLSNLIQEILDFDQA; QQIKCFNSKLFLCSICFCEKLGSECMYFLECRHVYCKACLKDYFEIQIRDGQVQCLNCPEPKCPSVATP; GQVKELVEAELFARYDRLLLQSSLDLMADVVYCPRPCCQLPVMQEPGCTMGICSSCNFAFCTLCRLTYH; GVSPCKVTAEKLMDLRNEYLQADEANKRLLDQRYGKRVIQKALEEMESKEWLEKNSKSCPCCGTPIEKL; DGCNKMTCTGCMQYFCWICMGSLSRANPYKHFNDPGSPCFNRLFYAVDVDDDIWEDEVED; TRTRPLEQKLISEEDLAANDILDYKDDDDKV; ; Recombinant protein using RC223420 also available, TP323420
• Chromatograms: Chromatograms ; Sequencher program is needed, download here
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_183401
• ORF Size: 1424 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_183401.3
• RefSeq Size: 4201 bp
• RefSeq ORF: 1425 bp
• Locus ID: 9604
• UniProt ID: Q9UBS8
• Cytogenetics: 5q31.3
• Protein Families: Druggable Genome, Transcription Factors
• MW: 53.8 kDa
• Summary: The protein encoded by this gene contains a RING zinc finger, a motif known to be involved in protein-protein interactions. This protein interacts with androgen receptor (AR) and may function as a coactivator that induces AR target gene expression in prostate. A dominant negative mutant of this gene has been demonstrated to inhibit the AR-mediated growth of prostate cancer. This protein also interacts with class III ubiquitin-conjugating enzymes (E2s) and may act as a ubiquitin-ligase (E3) in the ubiquitination of certain nuclear proteins. Six alternatively spliced transcript variants encoding two distinct isoforms have been reported. [provided by RefSeq, Jan 2011]