FREM1 (NM_144966) Human Tagged ORF Clone

SKU RC222886

FREM1 (Myc-DDK-tagged)-Human FRAS1 related extracellular matrix 1 (FREM1), transcript variant 1

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: FREM1
• Synonyms: BNAR; C9orf143; C9orf145; C9orf154; MOTA; TILRR; TRIGNO2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC222886 representing NM_144966; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGAACTCTCTGAGTTGGGGGGCTGCGAATGCCGTGCTGCTGCTGCTCCTCCTGGCCTGGGCCAGCCCCA; CCTTCATCAGCATCAACCGCGGGGTGAGGGTGATGAAGGGCCACTCTGCCTTCCTGTCAGGAGATGACCT; GAAGTTTGCCATCCCTAAAGAGAAAGATGCCTGCAAAGTGGAAGTTGTGATGAATGAGCCAATAACCCAG; AGGGTTGGGAAACTCACTCCACAGGTCTTTGACTGCCATTTCCTTCCCAACGAAGTCAAGTATGTTCACA; ATGGTTGTCCAATTCTTGATGAAGACACAGTGAAGCTCAGACTTTACAGATTTACTGAAAGAGATACCTT; CATAGAAACTTTTATCCTGTGGGTCTATCTCCTGGAACCAGACTGTAACATCATCCATATGAGTAACAAT; GTGCTGGAGGTGCCTGAATTCAATGGCTTGTCCCAAGCGATTGATAAAAATCTGCTCAGATTCGATTATG; ATAGGATGGCTAGCCTGGAATGTACCGTCAGCCTGGACACTGCGAGAACTCGGCTGCCAGCCCATGGCCA; GATGGTTCTCGGGGAGCCTCGACCAGAAGAACCTCGTGGAGATCAGCCACACAGTTTTTTCCCAGAGTCC; CAACTGAGAGCAAAACTGAAGTGTCCAGGTGGGAGCTGTACCCCAGGATTAAAGAAAATAGGAAGCCTCA; AAGTGAGCTGTGAGGAGTTCCTGCTGATGGGCCTTCGTTATCAGCATCTGGATCCCCCTTCACCCAACAT; TGATTATATCTCCATCCAACTGGACCTCACAGATACCAGGAGCAAAATTGTGTACAAGTCAGAGAGTGCG; TGGCTGCCTGTCTATATCAGAGCTGGAATTCCGAATCAGATTCCAAAGGCTGCATTCATGGCCGTGTTTA; TTCTGGAAGTGGATCAGTTCATCCTGACCTCCTTGACTACATCAGTTCTGGACTGTGAAGAAGATGAGAC; CCCTAAACCCTTGCTGGTGTTCAACATTACTAAAGCCCCGCTCCAGGGCTATGTGACTCACCTGTTGGAT; CACACCAGACCAATCTCCTCATTCACCTGGAAAGATCTCAGTGACATGCAGATCGCCTATCAGCCACCAA; ACAGCAGCCATTCTGAGAGGAGACATGATGAGGTAGAATTGGAGGTATACGACTTCTTCTTTGAAAGGAG; TGCACCTATGACAGTCCACATCTCCATCAGAACAGCAGATACAAATGCCCCCCGTGTATCCTGGAATACA; GGTCTGAGTCTCCTTGAGGGGCAGTCTCGAGCCATCACTTGGGAACAGTTTCAGGTTGTCGACAATGACG; ACATTGGTGCTGTCCGGCTAGTCACCGTTGGTGGCCTGCAGCATGGATGGCTGACTTTAAGAGGGGGGAA; AGGGTTTCTCTTCACCGTGGCTGACCTCCAGGCTGGAGTTGTTCGCTATCATCATGATGACAGCGACTCC; ACCAAAGACTTCGTGGTCTTCCGGATATTTGATGGCCATCACAGCATCCGTCACAAATTCCCCATCAACG; TCTTGCCCAAAGATGATAGTCCCCCGTTCCTCATAACCAATGTTGTGATTGAACTGGAGGAGGGGCAGAC; CATCCTGATCCAGGGATCCATGCTGCGAGCTTCAGATGTGGACGCCAGTGATGACTACATCTTCTTCAAT; ATCACAAAGCCTCCACAGGCTGGGGAGATCATGAAGAAGCCAGGGCCAGGACTGATAGGCTATCCTGTCC; ATGGCTTCCTTCAGAGGGATTTGTTTAATGGAATCATTTATTATCGTCATTTTGGTGGAGAAATCTTTGA; AGATTCTTTTCAATTTGTCCTGTGGGACAGCCATGAACCTCCAAATCTTTCAGTGCCACAGGTGGCAACA; ATCCATATAACTCCAGTGGATGACCAGCTTCCAAAAGAGGCTCCTGGAGTTTCTCGGCATTTGGTTGTCA; AGGAAACTGAGGTGGCCTATATAACTAAGAAACAGCTACATTTTATAGATTCAGAATCATATGACAGGGA; GCTGGTCTACACAATAACTACTCCTCCATTTTTCTCCTTCAGCCACAGACACTTGGATGCTGGGAAATTA; TTTATGGTGGACAGCATACCAAAAGTAGTTAAGAATCCTACGGCCCTGGAGCTGAGGTCATTCACTCAGC; ATGCTGTGAACTATATGAAAGTGGCCTACATGCCCCCCATGCAAGACATTGGTCCCCATTGCAGAGATGT; CCAGTTCACATTTTCTGTCAGTAACCAACATGGCGGTACTTTGCATGGGATCTGCTTTAACATTACAATC; CTCCCAGTGGACAATCAAGTTCCTGAGGCGTTCACCAACCCTCTGAAAGTGACTGAGGGAGGTCAAAGCA; TCATCAGCACAGAGCACATTCTAATTTCTGATGCAGATACCAAGCTGGACAATATTGACCTCTCCCTGCG; GGAATTGCCTCTGCACGGAAGGGTGGAGCTGAATGGATTTCCTCTAAATTCAGGGGGCACATTTTCTTGG; GGCGATCTCCATACCTTAAAAGTTAGGTATCAACATGATGGAACTGAAGTTCTTCAGGATGACCTACTCT; TGGAGGTCACCGATGGCACAAATTCAGCAGAATTTGTACTACATGTTGAGGTATTCCCTGTCAACGATGA; GCCACCAGTCTTAAAGGCTGACCTCATGCCTGTCATGAATTGCTCAGAGGGAGGAGAGGTGGTCATCACC; TCTGAATACATTTTTGCTACTGATGTGGACAGCGATAACTTGAAGTTGATGTTTGTGATTGCTCGCGAAC; CTCAGCATGGGGTGGTGAGGAGAGCTGGAGTCACAGTGGATCAGTTCTCTCAGAGAGATGTTATCTCAGA; GGCCGTGACATACAAACACACAGGTGGCGAGATTGGCCTGATGCCTTGTTTTGACACCATTACATTGGTG; GTTTCGGATGGAGAGGCTGGCCCTTTTGTGAATGGCTGTTGCTACAATGGACCCAATCCATCTGTTCCAC; TTCATGCGTCCTTTCCAGTGTATGATCTCAACATCACGGTATACCCAGTAGACAACCAGCCACCTTCCAT; TGCAATAGGTCCCGTGTTTGTTGTAGATGAGGGCTGCTCAACAGCCCTTACTGTTAACCATTTGTCCGCC; ACTGACCCTGACACTGCAGCAGATGACTTGGAATTTGTTTTGGTTTCTCCTCCTCAGTTTGGCTACCTCG; AAAATATACTCCCTTCTGTGGGTTTTGAAAAAAGCAATATTGGCATAAGTATAGATTCATTTCAGTGGAA; AGACATGAACGCTTTTCACATTAACTATGTGCAGTCCAGGCATCTGAGGATAGAACCAACTGCCGACCAG; TTCACGGTGTACGTCACAGATGGGAAGCATCACTCCTTGGAGATACCATTTTCTATTATAATCAACCCCA; CAAATGATGAAGCTCCTGACTTTGTAGTGCAGAATATTACTGTGTGTGAGGGTCAGATGAAAGAGCTGGA; CTCTTCCATCATCAGCGCTGTGGACCTGGACATTCCCCAGGATGCCCTGCTGTTCAGCATCACTCAAAAG; CCACGCCATGGCCTCCTCATCGATAGGGGGTTTAGCAAAGACTTCTCTGAGAATAAGCAGCCAGCCAACC; CTCACCAGAAACATGCACCTGTTCACAGCTTTTCCATGGAACTCCTCAAGACTGGAATGAGGTTGACGTA; CATGCATGATGACTCAGAGAGCCTTGCTGATGATTTTACAATCCAATTGTCAGATGGGAAACATAAGATA; CTTAAAACCATTTCAGTAGAGGTCATCCCAGTTAATGATGAAAAACCAATGCTGAGCAAGAAGGCTGAAA; TTGCAATGAATATGGGTGAAACTCGTATTATTTCCAGTGCTATTCTTTCAGCCATAGATGAAGACTCACC; CAGGGAGAAGATTTACTATGTATTTGAAAGGCTTCCCCAAAATGGGCAACTTCAGCTTAAGATAGGGAGG; GACTGGGTTCCTCTCTCCCCTGGCATGAAATGCACTCAGGAGGAAGTGGATCTGAACTTGCTGAGATACA; CACACACCGGGGCAATGGATTCCCAGAATCAAGATAGCTTCACCTTCTACCTTTGGGATGGCAACAACAG; GTCCCCTGCTCTTGACTGTCAAATCACCATCAAGGACATGGAAAAAGGTGATATTGTCATCCTTACAAAA; CCACTCGTGGTGTCTAAAGGTGATAGAGGTTTCTTAACAACCACCACGCTCCTGGCTGTGGACGGAACAG; ACAAGCCTGAGGAACTGCTCTATGTCATCACCTCCCCTCCGCGATATGGCCAGATCGAATATGTTCACTA; TCCTGGAGTTCCCATTACAAACTTCAGCCAAATGGATGTAGTGGGGCAGACAGTTTGCTATGTACACAAG; AGCAAGGTGACTGTCTCCAGTGACAGATTCAGATTCATCATCAGCAATGGACTGCGGACCGAGCACGGGG; TGTTTGAGATCACACTGGAGACTGTGGACAGAGCCCTGCCTGTGGTAACCAGGAACAAGGGGTTGAGACT; GGCCCAAGGGGCCGTGGGCCTGCTTTCCCCTGACCTCCTTCAGCTGACCGACCCTGATACACCTGCGGAG; AACCTCACCTTCCTCTTGGTTCAGCTCCCCCAGCATGGCCAGCTCTACCTGTGGGGGACAGGGCTACTTC; AACACAATTTCACCCAGCAGGATGTGGACAGCAAGAATGTGGCCTATCGGCACTCAGGAGGGGACTCCCA; GACTGACTGCTTTACTTTCATGGCCACAGATGGGACAAACCAAGGCTTTATTGTGAATGGGAGAGTGTGG; GAAGAACCTGTTTTATTCACCATTCAGGTAGACCAATTGGACAAAACAGCTCCTCGTATCACACTCTTGC; ATTCCCCTTCTCAAGTGGGGCTCCTGAAAAATGGCTGCTACGGGATTTACATCACTTCCCGCGTGTTGAA; GGCATCAGACCCTGACACTGAGGACGATCAGATCATCTTTAAAATTCTACAAGGCCCAAAACATGGACAT; CTGGAGAACACAACAACAGGTGAATTTATCCATGAGAAATTTAGCCAAAAGGACTTAAACAGTAAGACTA; TTCTTTACATCATAAACCCATCTTTGGAAGTAAATTCAGATACCGTGGAATTTCAAATCATGGACCCCAC; AGGGAACTCGGCCACTCCTCAAATTTTGGAACTGAAGTGGTCTCATATTGAATGGTCACAGACCGAATAT; GAAGTCTGTGAGAATGTGGGTTTGTTGCCCTTGGAAATTATCAGAAGGGGATATTCCATGGACTCGGCCT; TTGTGGGTATAAAGGTCAACCAAGTGTCAGCTGCAGTTGGAAAAGATTTCACCGTGATTCCATCTAAACT; GATTCAGTTTGACCCAGGAATGTCAACTAAGATGTGGAATATAGCAATTACCTATGACGGATTAGAGGAA; GATGATGAGGTCTTTGAAGTAATTCTGAACTCCCCTGTGAATGCAGTTCTTGGCACAAAGACAAAAGCTG; CAGTGAAAATTTTGGACTCAAAAGGAGGACAATGCCATCCTTCATATTCCTCCAACCAAAGCAAGCACAG; CACATGGGAGAAGGGCATTTGGCATCTGCTGCCCCCAGGGTCTTCCTCATCCACCACTTCTGGTTCCTTT; CATCTGGAAAGAAGACCTCTTCCATCTTCCATGCAGCTAGCAGTCATCAGGGGAGACACCCTGCGGGGCT; TTGATTCTACAGATCTTTCTCAAAGGAAGCTTAGGACCCGTGGGAATGGCAAAACAGTTCGTCCATCCTC; TGTTTATAGAAATGGAACAGACATCATCTATAATTATCATGGGATAGTTTCCTTGAAACTGGAGGATGAC; AGTTTCCCAACTCACAAAAGGAAGGCCAAAGTATCCATCATTAGTCAGCCACAAAAGACAATCAAAGTGG; CAGAACTGCCTCAAGCAGATAAGGTGGAATCCACAACTGACTCACACTTCCCCAGACAGGACCAGTTGCC; CTCATTTCCAAAGAACTGCACTCTGGAATTAAAGGGACTCTTCCATTTTGAAGAAGGCATCCAGAAGCTG; TATCAGTGCAATGGGATCGCCTGGAAAGCCTGGAGTCCCCAAACCAAGGATGTGGAAGACAAATCCTGTC; CAGCCGGGTGGCACCAGCACTCAGGCTACTGTCACATCTTGATCACAGAGCAGAAAGGCACCTGGAATGC; GGCTGCCCAAGCTTGCAGGGAACAATACCTGGGCAACCTTGTAACTGTATTCTCCAGGCAGCACATGCGG; TGGCTCTGGGACATTGGTGGGAGAAAGTCCTTTTGGATAGGTTTGAACGACCAAGTGCATGCTGGCCACT; GGGAGTGGATCGGTGGTGAACCTGTTGCCTTCACCAATGGGAGAAGAGGGCCCTCTCAACGCTCCAAGCT; TGGAAAGAGCTGTGTTTTGGTTCAAAGACAAGGGAAATGGCAAACAAAAGACTGTAGGAGAGCCAAACCT; CATAATTATGTGTGTTCCAGAAAACTC; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC222886 representing NM_144966; Red=Cloning site Green=Tags(s); ; MNSLSWGAANAVLLLLLLAWASPTFISINRGVRVMKGHSAFLSGDDLKFAIPKEKDACKVEVVMNEPITQ; RVGKLTPQVFDCHFLPNEVKYVHNGCPILDEDTVKLRLYRFTERDTFIETFILWVYLLEPDCNIIHMSNN; VLEVPEFNGLSQAIDKNLLRFDYDRMASLECTVSLDTARTRLPAHGQMVLGEPRPEEPRGDQPHSFFPES; QLRAKLKCPGGSCTPGLKKIGSLKVSCEEFLLMGLRYQHLDPPSPNIDYISIQLDLTDTRSKIVYKSESA; WLPVYIRAGIPNQIPKAAFMAVFILEVDQFILTSLTTSVLDCEEDETPKPLLVFNITKAPLQGYVTHLLD; HTRPISSFTWKDLSDMQIAYQPPNSSHSERRHDEVELEVYDFFFERSAPMTVHISIRTADTNAPRVSWNT; GLSLLEGQSRAITWEQFQVVDNDDIGAVRLVTVGGLQHGWLTLRGGKGFLFTVADLQAGVVRYHHDDSDS; TKDFVVFRIFDGHHSIRHKFPINVLPKDDSPPFLITNVVIELEEGQTILIQGSMLRASDVDASDDYIFFN; ITKPPQAGEIMKKPGPGLIGYPVHGFLQRDLFNGIIYYRHFGGEIFEDSFQFVLWDSHEPPNLSVPQVAT; IHITPVDDQLPKEAPGVSRHLVVKETEVAYITKKQLHFIDSESYDRELVYTITTPPFFSFSHRHLDAGKL; FMVDSIPKVVKNPTALELRSFTQHAVNYMKVAYMPPMQDIGPHCRDVQFTFSVSNQHGGTLHGICFNITI; LPVDNQVPEAFTNPLKVTEGGQSIISTEHILISDADTKLDNIDLSLRELPLHGRVELNGFPLNSGGTFSW; GDLHTLKVRYQHDGTEVLQDDLLLEVTDGTNSAEFVLHVEVFPVNDEPPVLKADLMPVMNCSEGGEVVIT; SEYIFATDVDSDNLKLMFVIAREPQHGVVRRAGVTVDQFSQRDVISEAVTYKHTGGEIGLMPCFDTITLV; VSDGEAGPFVNGCCYNGPNPSVPLHASFPVYDLNITVYPVDNQPPSIAIGPVFVVDEGCSTALTVNHLSA; TDPDTAADDLEFVLVSPPQFGYLENILPSVGFEKSNIGISIDSFQWKDMNAFHINYVQSRHLRIEPTADQ; FTVYVTDGKHHSLEIPFSIIINPTNDEAPDFVVQNITVCEGQMKELDSSIISAVDLDIPQDALLFSITQK; PRHGLLIDRGFSKDFSENKQPANPHQKHAPVHSFSMELLKTGMRLTYMHDDSESLADDFTIQLSDGKHKI; LKTISVEVIPVNDEKPMLSKKAEIAMNMGETRIISSAILSAIDEDSPREKIYYVFERLPQNGQLQLKIGR; DWVPLSPGMKCTQEEVDLNLLRYTHTGAMDSQNQDSFTFYLWDGNNRSPALDCQITIKDMEKGDIVILTK; PLVVSKGDRGFLTTTTLLAVDGTDKPEELLYVITSPPRYGQIEYVHYPGVPITNFSQMDVVGQTVCYVHK; SKVTVSSDRFRFIISNGLRTEHGVFEITLETVDRALPVVTRNKGLRLAQGAVGLLSPDLLQLTDPDTPAE; NLTFLLVQLPQHGQLYLWGTGLLQHNFTQQDVDSKNVAYRHSGGDSQTDCFTFMATDGTNQGFIVNGRVW; EEPVLFTIQVDQLDKTAPRITLLHSPSQVGLLKNGCYGIYITSRVLKASDPDTEDDQIIFKILQGPKHGH; LENTTTGEFIHEKFSQKDLNSKTILYIINPSLEVNSDTVEFQIMDPTGNSATPQILELKWSHIEWSQTEY; EVCENVGLLPLEIIRRGYSMDSAFVGIKVNQVSAAVGKDFTVIPSKLIQFDPGMSTKMWNIAITYDGLEE; DDEVFEVILNSPVNAVLGTKTKAAVKILDSKGGQCHPSYSSNQSKHSTWEKGIWHLLPPGSSSSTTSGSF; HLERRPLPSSMQLAVIRGDTLRGFDSTDLSQRKLRTRGNGKTVRPSSVYRNGTDIIYNYHGIVSLKLEDD; SFPTHKRKAKVSIISQPQKTIKVAELPQADKVESTTDSHFPRQDQLPSFPKNCTLELKGLFHFEEGIQKL; YQCNGIAWKAWSPQTKDVEDKSCPAGWHQHSGYCHILITEQKGTWNAAAQACREQYLGNLVTVFSRQHMR; WLWDIGGRKSFWIGLNDQVHAGHWEWIGGEPVAFTNGRRGPSQRSKLGKSCVLVQRQGKWQTKDCRRAKP; HNYVCSRKL; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Chromatograms: Chromatograms ; Sequencher program is needed, download here
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_144966
• ORF Size: 6537 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_144966.7
• RefSeq Size: 7387 bp
• RefSeq ORF: 6540 bp
• Locus ID: 158326
• UniProt ID: Q5H8C1
• Cytogenetics: 9p22.3
• Protein Families: Protease, Transmembrane
• MW: 241.9 kDa
• Summary: This gene encodes a basement membrane protein that may play a role in craniofacial and renal development. Mutations in this gene have been associated with bifid nose with or without anorectal and renal anomalies. Alternatively spliced transcript variants encoding different isoforms have been described. PubMed ID 19940113 describes one such variant that initiates transcription within a distinct, internal exon; the resulting shorter isoform (named Toll-like/interleukin-1 receptor regulator, TILRR) is suggested to be a co-receptor of the interleukin 1 receptor family and may regulate receptor function and Toll-like receptor/interleukin 1 receptor signal transduction, contributing to the control of inflammatory response activation. [provided by RefSeq, Apr 2011]