UNG (NM_080911) Human Tagged ORF Clone
$450.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | UNG |
| Synonyms | DGU; HIGM4; HIGM5; UDG; UNG1; UNG2; UNG15 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_080911 |
| ORF Size | 939 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_080911.3 |
| RefSeq Size | 2053 bp |
| RefSeq ORF | 942 bp |
| Locus ID | 7374 |
| UniProt ID | P13051 |
| Cytogenetics | 12q24.11 |
| Domains | UDG |
| Protein Families | Druggable Genome, Stem cell - Pluripotency |
| Protein Pathways | Base excision repair, Primary immunodeficiency |
| MW | 34.5 kDa |
| Summary | This gene encodes one of several uracil-DNA glycosylases. One important function of uracil-DNA glycosylases is to prevent mutagenesis by eliminating uracil from DNA molecules by cleaving the N-glycosylic bond and initiating the base-excision repair (BER) pathway. Uracil bases occur from cytosine deamination or misincorporation of dUMP residues. Alternative promoter usage and splicing of this gene leads to two different isoforms: the mitochondrial UNG1 and the nuclear UNG2. The UNG2 term was used as a previous symbol for the CCNO gene (GeneID 10309), which has been confused with this gene, in the literature and some databases. provided by RefSeq, Nov 2010 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_080911" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC222868L1 | Lenti ORF clone of Human uracil-DNA glycosylase (UNG), transcript variant 2, Myc-DDK-tagged | 10 ug |
$750.00
|
|
| RC222868L2 | Lenti ORF clone of Human uracil-DNA glycosylase (UNG), transcript variant 2, mGFP tagged | 10 ug |
$750.00
|
|
| RC222868L3 | Lenti ORF clone of Human uracil-DNA glycosylase (UNG), transcript variant 2, Myc-DDK-tagged | 10 ug |
$750.00
|
|
| RC222868L4 | Lenti ORF clone of Human uracil-DNA glycosylase (UNG), transcript variant 2, mGFP tagged | 10 ug |
$750.00
|
|
| RG222868 | UNG (tGFP-tagged) - Human uracil-DNA glycosylase (UNG), transcript variant 2 | 10 ug |
$650.00
|
|
| SC109875 | UNG (untagged)-Human uracil-DNA glycosylase (UNG), transcript variant 2 | 10 ug |
$450.00
|
|
Citations
Isoliquiritigenin alleviates experimental autoimmune encephalomyelitis by modulating inflammatory and neuroprotective reactive astrocytes
Zhang, Qu, Song et al
Biomed Pharmacother (2024) 178, 117188 DOI: 10.1016/j.biopha.2024.117188
Zhang, Qu, Song et al
Biomed Pharmacother (2024) 178, 117188 DOI: 10.1016/j.biopha.2024.117188
Cited by 12
AI Summary: OriGene’s mouse monoclonal anti-β-actin antibody (TA811000S) was used as an internal control in western blot analyses to assess GFAP levels in astrocytes, helping to evaluate the effects of isoliquiritigenin on astrocyte activation in this study.
Snippet: Once primary astrocytes on slides in a 24-well plate reached 60–70 % confluence, the cell culture supernatants prepared in Section 2.9 were added to the corresponding wells. GFAP levels were assessed by western blotting after 24 hours using standard methods [47] , [48] , with β-actin serving as an internal reference, detected by staining with mouse monoclonal anti-β-actin (TA811000S, OriGene, US)
Loss of WIPI4 in neurodegeneration causes autophagy-independent ferroptosis
Zhu, Fujimaki, Snape et al
Nat Cell Biol (2024)
Zhu, Fujimaki, Snape et al
Nat Cell Biol (2024)
Cited by 46
AI Summary: OriGene's PISD-myc-DDK isoform d (RC222868) plasmid was used as the template to generate a mitochondrial-only PISD-myc-DDK construct for investigating the molecular mechanisms underlying WIPI4-associated neurodegeneration in this study.
Snippet: The primers used are listed in Supplementary Table 2. Mitochondrial-only PISD-myc-DDK was made from PISD-myc-DDK isoform d (Origene, RC222868) by mutating three leucine residues, required for lipid droplet localization, to positively charged residues (I80K, I89K, I90R)
Unprocessed Genomic Uracil as a Source of DNA Replication Stress in Cancer Cells
Saxena, Patel, Nabel et al
bioRxiv (2024)
Saxena, Patel, Nabel et al
bioRxiv (2024)
Cited by 1
Snippet: The human UNG ORF clone was purchased from Origene (Catalog#: RC222868). From this plasmid, UNG2 was amplified and cloned into the pENTR-TOPO Gateway vector. For lentiviral expression, pENTR-TOPO-UNG2 was inserted into pINDUCER20 carrying an HA tag at the C terminus. The endogenous stop codon of UNG2 was removed to
Showing 1-3 of 3 papers.
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