RUNX1T1 (NM_175635) Human Tagged ORF Clone

SKU RC222426

RUNX1T1 (Myc-DDK-tagged)-Human runt-related transcription factor 1, translocated to, 1 (cyclin D-related) (RUNX1T1), transcript variant 3

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: RUNX1T1
• Synonyms: AML1-MTG8; AML1T1; CBFA2T1; CDR; ETO; MTG8; ZMYND2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC222426 representing NM_175635; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGCCAGACTCACCTGTGGATGTGAAGACGCAATCTAGGCTGACTCCTCCAACAATGCCACCTCCCCCAA; CTACTCAAGGAGCTCCAAGAACCAGTTCATTTACACCGACAACGTTAACTAATGGCACGAGCCATTCTCC; TACAGCCTTGAATGGCGCCCCCTCACCACCCAATGGCTTCAGCAATGGGCCTTCCTCTTCTTCCTCCTCC; TCTCTGGCTAATCAACAGCTGCCCCCAGCCTGTGGTGCCAGGCAACTCAGCAAGCTGAAAAGGTTCCTTA; CTACCCTGCAGCAGTTTGGCAATGACATTTCACCCGAGATAGGAGAAAGAGTTCGCACCCTCGTTCTGGG; ACTAGTGAACTCCACTTTGACAATTGAAGAATTTCATTCCAAACTGCAAGAAGCTACTAACTTCCCACTG; AGACCTTTTGTCATCCCATTTTTGAAGGCCAACTTGCCCCTGCTGCAGCGTGAGCTCCTCCACTGCGCAA; GACTGGCCAAACAGAACCCTGCCCAGTACCTCGCCCAGCATGAACAGCTGCTTCTGGATGCCAGCACCAC; CTCACCTGTTGACTCCTCAGAGCTGCTTCTCGATGTGAACGAAAACGGGAAGAGGCGAACTCCAGACAGA; ACCAAAGAAAATGGCTTTGACAGAGAGCCTTTGCACTCAGAACATCCAAGCAAGCGACCATGCACTATTA; GCCCAGGCCAGCGGTACAGTCCAAATAACGGCTTATCCTACCAGCCCAATGGCCTGCCTCACCCTACCCC; ACCTCCACCTCAGCATTACCGTTTGGATGATATGGCCATTGCCCACCACTACAGGGACTCCTATCGACAC; CCCAGCCACAGGGACCTCAGGGACAGAAACAGACCTATGGGGTTGCATGGCACACGTCAAGAAGAAATGA; TTGATCACAGACTAACAGACAGAGAATGGGCAGAAGAGTGGAAACATCTTGACCATCTGTTAAACTGCAT; AATGGACATGGTAGAAAAAACAAGGCGATCTCTCACCGTACTAAGGCGGTGTCAAGAAGCAGACCGGGAA; GAATTGAATTACTGGATCCGGCGGTACAGTGACGCCGAGGACTTAAAAAAAGGTGGCGGCAGTAGCAGCA; GCCACTCTAGGCAGCAGAGTCCCGTCAACCCAGACCCAGTTGCACTAGACGCGCATCGGGAATTCCTTCA; CAGGCCTGCGTCTGGATACGTGCCAGAGGAGATCTGGAAGAAAGCTGAGGAGGCCGTCAATGAGGTGAAG; CGCCAGGCGATGACGGAGCTGCAGAAGGCCGTGTCTGAGGCGGAGCGGAAAGCCCACGACATGATCACAA; CAGAGAGGGCCAAGATGGAGCGCACGGTCGCCGAGGCCAAACGGCAGGCGGCGGAGGACGCACTGGCAGT; TATCAATCAGCAGGAGGATTCAAGCGAGAGTTGCTGGAATTGTGGCCGTAAAGCGAGTGAAACCTGCAGT; GGCTGTAACACAGCCCGATACTGTGGCTCATTTTGCCAGCACAAAGACTGGGAGAAGCACCATCACATCT; GTGGACAGACCCTGCAGGCCCAGCAGCAGGGAGACACACCTGCAGTCAGCTCCTCTGTCACGCCCAACAG; CGGGGCTGGGAGCCCGATGGACACACCACCAGCAGCCACTCCGAGGTCAACCACCCCGGGAACCCCTTCC; ACCATAGAGACAACCCCTCGC; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC222426 representing NM_175635; Red=Cloning site Green=Tags(s); ; MPDSPVDVKTQSRLTPPTMPPPPTTQGAPRTSSFTPTTLTNGTSHSPTALNGAPSPPNGFSNGPSSSSSS; SLANQQLPPACGARQLSKLKRFLTTLQQFGNDISPEIGERVRTLVLGLVNSTLTIEEFHSKLQEATNFPL; RPFVIPFLKANLPLLQRELLHCARLAKQNPAQYLAQHEQLLLDASTTSPVDSSELLLDVNENGKRRTPDR; TKENGFDREPLHSEHPSKRPCTISPGQRYSPNNGLSYQPNGLPHPTPPPPQHYRLDDMAIAHHYRDSYRH; PSHRDLRDRNRPMGLHGTRQEEMIDHRLTDREWAEEWKHLDHLLNCIMDMVEKTRRSLTVLRRCQEADRE; ELNYWIRRYSDAEDLKKGGGSSSSHSRQQSPVNPDPVALDAHREFLHRPASGYVPEEIWKKAEEAVNEVK; RQAMTELQKAVSEAERKAHDMITTERAKMERTVAEAKRQAAEDALAVINQQEDSSESCWNCGRKASETCS; GCNTARYCGSFCQHKDWEKHHHICGQTLQAQQQGDTPAVSSSVTPNSGAGSPMDTPPAATPRSTTPGTPS; TIETTPR; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Chromatograms: Chromatograms ; Sequencher program is needed, download here
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_175635
• ORF Size: 1701 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_175635.3
• RefSeq Size: 3233 bp
• RefSeq ORF: 1704 bp
• Locus ID: 862
• UniProt ID: Q06455
• Cytogenetics: 8q21.3
• Protein Families: Transcription Factors
• Protein Pathways: Acute myeloid leukemia, Pathways in cancer
• MW: 63 kDa
• Summary: This gene encodes a member of the myeloid translocation gene family which interact with DNA-bound transcription factors and recruit a range of corepressors to facilitate transcriptional repression. The t(8;21)(q22;q22) translocation is one of the most frequent karyotypic abnormalities in acute myeloid leukemia. The translocation produces a chimeric gene made up of the 5'-region of the runt-related transcription factor 1 gene fused to the 3'-region of this gene. The chimeric protein is thought to associate with the nuclear corepressor/histone deacetylase complex to block hematopoietic differentiation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2010]