Calpastatin (CAST) (NM_173061) Human Tagged ORF Clone

SKU RC222278

CAST (Myc-DDK-tagged)-Human calpastatin (CAST), transcript variant 3

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: Calpastatin
• Synonyms: BS-17; calpain inhibitor; calpastatin; heart-type calpastatin; MGC9402; OTTHUMP00000158519; OTTHUMP00000158520; sperm BS-17 component
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC222278 representing NM_173061; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGGCCAGTTTCTATCTTCGACTTTCTTGGAGGGCTCACCGGCCACAGTGTGGCACGATAAGCTTTGTG; ACGGTGAACGCAGAGGAGCAAGAGAAGCAGTTCGTATCTTCCAGGACCAAGCAAAAGCTAAAGAAGAAAA; ACTAGAGAAGTGTGGTGAGGATGATGAAACAATCCCATCTGAGTACAGATTAAAACCAGCCACGGATAAA; GATGGAAAACCACTATTGCCAGAGCCTGAAGAAAAACCCAAGCCTCGGAGTGAATCAGAACTCATTGATG; AACTTTCAGAAGATTTTGACCGGTCTGAATGTAAAGAGAAACCATCTAAGCCAACTGAAAAGACAGAAGA; ATCTAAGGCCGCTGCTCCAGCTCCTGTGTCGGAGGCTGTGTGTCGGACCTCCATGTGTAGTATACAGTCA; GCACCCCCTGAGCCGGCTACCTTGAAGGGCACAGTGCCAGATGATGCTGTAGAAGCCTTGGCTGATAGCC; TGGGGAAAAAGGAAGCAGATCCAGAAGATGGAAAACCTGTGATGGATAAAGTCAAGGAGAAGGCCAAAGA; AGAAGACCGTGAAAAGCTTGGTGAAAAAGAAGAAACAATTCCTCCTGATTATAGATTAGAAGAGGTCAAG; GATAAAGATGGAAAGCCACTCCTGCCAAAAGAGTCTAAGGAACAGCTTCCACCCATGAGTGAAGACTTCC; TTCTGGATGCTTTGTCTGAGGACTTCTCTGGTCCACAAAATGCTTCATCTCTTAAATTTGAAGATGCTAA; ACTTGCTGCTGCCATCTCTGAAGTGGTTTCCCAAACCCCAGCTTCAACGACCCAAGCTGGAGCCCCACCC; CGTGATACCTCGAGTGACAAAGACCTCGATGATGCCTTGGATAAACTCTCTGACAGTCTAGGACAAAGGC; AGCCTGACCCAGATGAGAACAAACCAATGGAAGATAAAGTAAAGGAAAAAGCTAAAGCTGAACATAGAGA; CAAGCTTGGAGAAAGAGATGACACTATCCCACCTGAATACAGACATCTCCTGGATGATAATGGACAGGAC; AAACCAGTGAAGCCACCTACAAAGAAATCAGAGGATTCAAAGAAACCTGCAGATGACCAAGACCCCATTG; ATGCTCTCTCAGGAGATCTGGACAGCTGTCCCTCCACTACAGAAACCTCACAGAACACAGCAAAGGATAA; GTGCAAGAAGGCTGCTTCCAGCTCCAAAGCACCTAAGAATGGAGGTAAAGCGAAGGATTCAGCAAAGACA; ACAGAGGAAACTTCCAAGCCAAAAGATGAC; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC222278 representing NM_173061; Red=Cloning site Green=Tags(s); ; MGQFLSSTFLEGSPATVWHDKLCDGERRGAREAVRIFQDQAKAKEEKLEKCGEDDETIPSEYRLKPATDK; DGKPLLPEPEEKPKPRSESELIDELSEDFDRSECKEKPSKPTEKTEESKAAAPAPVSEAVCRTSMCSIQS; APPEPATLKGTVPDDAVEALADSLGKKEADPEDGKPVMDKVKEKAKEEDREKLGEKEETIPPDYRLEEVK; DKDGKPLLPKESKEQLPPMSEDFLLDALSEDFSGPQNASSLKFEDAKLAAAISEVVSQTPASTTQAGAPP; RDTSSDKDLDDALDKLSDSLGQRQPDPDENKPMEDKVKEKAKAEHRDKLGERDDTIPPEYRHLLDDNGQD; KPVKPPTKKSEDSKKPADDQDPIDALSGDLDSCPSTTETSQNTAKDKCKKAASSSKAPKNGGKAKDSAKT; TEETSKPKDD; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Chromatograms: Chromatograms ; Sequencher program is needed, download here
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_173061
• ORF Size: 1290 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_173061.2, NP_775084.1
• RefSeq Size: 1889 bp
• RefSeq ORF: 1292 bp
• Locus ID: 831
• Cytogenetics: 5q15
• MW: 46.9 kDa
• Summary: The protein encoded by this gene is an endogenous calpain (calcium-dependent cysteine protease) inhibitor. It consists of an N-terminal domain L and four repetitive calpain-inhibition domains (domains 1-4), and it is involved in the proteolysis of amyloid precursor protein. The calpain/calpastatin system is involved in numerous membrane fusion events, such as neural vesicle exocytosis and platelet and red-cell aggregation. The encoded protein is also thought to affect the expression levels of genes encoding structural or regulatory proteins. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jun 2010]