Flt3 ligand (FLT3LG) (NM_001459) Human Tagged ORF Clone
SKU
RC222242
FLT3LG (Myc-DDK-tagged)-Human fms-related tyrosine kinase 3 ligand (FLT3LG), transcript variant 3
$300.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | Flt3 ligand |
| Synonyms | FL; FLG3L; FLT3L |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_001459 |
| ORF Size | 705 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_001459.4 |
| RefSeq Size | 1074 bp |
| RefSeq ORF | 708 bp |
| Locus ID | 2323 |
| UniProt ID | P49771 |
| Cytogenetics | 19q13.33 |
| Protein Families | Druggable Genome, ES Cell Differentiation/IPS, Secreted Protein, Transmembrane |
| Protein Pathways | Cytokine-cytokine receptor interaction, Hematopoietic cell lineage, Pathways in cancer |
| MW | 26.4 kDa |
| Summary | Dendritic cells (DCs) provide the key link between innate and adaptive immunity by recognizing pathogens and priming pathogen-specific immune responses. FLT3LG controls the development of DCs and is particularly important for plasmacytoid DCs and CD8 (see MIM 186910)-positive classical DCs and their CD103 (ITGAE; MIM 604682)-positive tissue counterparts (summary by Sathaliyawala et al., 2010 PubMed 20933441).supplied by OMIM, Jan 2011 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_001459" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC222242L1 | Lenti ORF clone of Human fms-related tyrosine kinase 3 ligand (FLT3LG), transcript variant 3, Myc-DDK-tagged | 10 ug |
$600.00
|
|
| RC222242L2 | Lenti ORF clone of Human fms-related tyrosine kinase 3 ligand (FLT3LG), transcript variant 3, mGFP tagged | 10 ug |
$600.00
|
|
| RC222242L3 | Lenti ORF clone of Human fms-related tyrosine kinase 3 ligand (FLT3LG), transcript variant 3, Myc-DDK-tagged | 10 ug |
$600.00
|
|
| RC222242L4 | Lenti ORF clone of Human fms-related tyrosine kinase 3 ligand (FLT3LG), transcript variant 3, mGFP tagged | 10 ug |
$600.00
|
|
| RG222242 | FLT3LG (tGFP-tagged) - Human fms-related tyrosine kinase 3 ligand (FLT3LG), transcript variant 3 | 10 ug |
$489.00
$500.00
|
|
| SC303027 | FLT3LG (untagged)-Human fms-related tyrosine kinase 3 ligand (FLT3LG), transcript variant 3 | 10 ug |
$300.00
|
|
Citations
Human myelocyte and metamyelocyte-stage neutrophils suppress tumor immunity and promote cancer progression
Liu, Shi, Lu et al
bioRxiv (2025)
Liu, Shi, Lu et al
bioRxiv (2025)
AI Summary: OriGene products, specifically Flt3L (Cat#: RC222242), were used to induce the trans-differentiation of myelocyte and metamyelocyte-stage neutrophils into monocytic cells, enabling the study of their role in tumor immunity and cancer progression.
Snippet: d from Origene company, and their product information is Flt3L (Cat#: RC222242) and G-C
A ROR1-HER3-lncRNA signalling axis modulates the Hippo-YAP pathway to regulate bone metastasis
Li, Wang, Xing et al
Nat. Cell Biol. (2017) 19 (2), 106-119 DOI: 10.1038/ncb3464
Li, Wang, Xing et al
Nat. Cell Biol. (2017) 19 (2), 106-119 DOI: 10.1038/ncb3464
Engineered niches support the development of human dendritic cells in humanized mice
Anselmi, Vaivode, Dutertre et al
bioRxiv (2019)
Anselmi, Vaivode, Dutertre et al
bioRxiv (2019)
Snippet: Human FLT3L, SCF and TPO were amplified by PCR from cDNA expression plasmids (Origene) and cloned into pMX retroviral vectors (vectors details in Supplementary Table 1). Lentiviral vector pBABE-puro-SDF-1 alpha was a gift from Bob Weinberg (Addgene plasmid #12270) 75. Viral particles were generated using the retroviral packaging plasmid pCL-Ampho and a second generation lentiviral packaging system
Showing 1-3 of 3 papers.
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