MYH14 (NM_001077186) Human Tagged ORF Clone

SKU RC221497

MYH14 (Myc-DDK-tagged)-Human myosin, heavy chain 14, non-muscle (MYH14), transcript variant 1

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: MYH14
• Synonyms: DFNA4; DFNA4A; FP17425; MHC16; MYH17; myosin; NMHC-II-C; NMHC II-C; PNMHH
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC221497 representing NM_001077186; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGCAGCCGTGACCATGTCGGTGCCCGGGCGGAAGGCGCCCCCCAGGCCGGGCCCAGTGCCCGAGGCGG; CCCAGCCGTTCCTGTTCACGCCCCGCGGGCCCAGCGCGGGTGGCGGGCCTGGCTCGGGCACCTCCCCGCA; GGTGGAGTGGACGGCCCGGCGTCTCGTGTGGGTGCCTTCGGAGCTTCACGGGTTCGAGGCGGCGGCGCTG; CGGGACGAAGGCGAGGAGGAGGCGGAGGTGGAGCTGGCGGAGAGCGGGAGGCGGCTGCGACTGCCGCGGG; ACCAGATCCAGCGCATGAACCCGCCCAAGTTCAGCAAGGCCGAGGACATGGCCGAGCTGACCTGCCTCAA; CGAGGCCTCGGTCCTGCACAACCTCCGGGAGCGGTACTACTCCGGCCTCATCTACACGTACTCCGGCCTT; TTCTGTGTGGTCATCAACCCGTACAAGCAGCTTCCCATCTACACAGAAGCCATTGTGGAGATGTACCGGG; GCAAGAAGCGCCACGAGGTGCCACCCCACGTGTACGCAGTGACCGAGGGGGCCTATCGGAGCATGCTGCA; GGATCGTGAGGACCAGTCCATTCTCTGCACTGGAGAGTCTGGAGCTGGGAAGACGGAAAACACCAAGAAG; GTCATCCAGTACCTCGCCCACGTGGCGTCGTCTCCAAAGGGCAGGAAGGAGCCGGGTGTCCCCGCCTCCG; TCAGCACCGTGTCTTATGGTGAGCTGGAGCGGCAGCTGCTTCAGGCCAACCCCATCCTAGAGGCCTTTGG; CAATGCCAAGACAGTGAAGAATGACAACTCCTCCCGATTCGGCAAATTCATCCGCATCAACTTTGATGTT; GCCGGGTACATCGTGGGCGCCAACATTGAGACCTACCTGCTGGAGAAGTCGCGGGCCATCCGCCAGGCCA; AGGACGAGTGCAGCTTCCACATCTTCTACCAGCTGCTGGGGGGCGCTGGAGAGCAGCTCAAAGCCGACCT; CCTCCTCGAGCCCTGCTCCCACTACCGGTTCCTGACCAACGGGCCGTCATCCTCTCCCGGCCAGGAGCGG; GAACTCTTCCAGGAGACGCTGGAGTCGCTGCGGGTCCTGGGATTCAGCCACGAGGAAATCATCTCCATGC; TGCGGATGGTCTCAGCAGTTCTCCAGTTTGGCAACATTGCCTTGAAGAGAGAACGGAACACCGATCAAGC; CACCATGCCTGACAACACAGCTGCACAGAAGCTCTGCCGCCTCTTGGGACTGGGGGTGACGGATTTCTCC; CGAGCCTTGCTCACCCCTCGCATCAAAGTTGGCCGAGACTATGTGCAGAAAGCCCAGACTAAGGAACAGG; CTGACTTCGCGCTGGAGGCCCTGGCCAAGGCCACCTACGAGCGCCTCTTCCGCTGGCTGGTTCTGCGCCT; CAACCGGGCCTTGGACCGCAGCCCCCGCCAAGGCGCCTCCTTCCTGGGCATCCTGGACATCGCGGGCTTT; GAGATCTTCCAGCTGAACTCCTTCGAGCAGCTCTGCATCAACTACACCAACGAGAAGCTGCAGCAGCTCT; TCAACCACACCATGTTCGTGCTGGAGCAGGAGGAGTACCAGCGTGAGGGCATCCCCTGGACCTTCCTCGA; CTTTGGCCTCGACCTGCAGCCCTGCATCGACCTCATCGAGCGGCCGGCCAACCCCCCTGGACTCCTGGCC; CTGCTGGATGAGGAGTGCTGGTTCCCGAAGGCCACAGACAAGTCGTTTGTGGAGAAGGTAGCCCAGGAGC; AGGGCGGCCACCCCAAGTTCCAGCGGCCGAGGCACCTGCGGGATCAGGCCGACTTCAGTGTTCTCCACTA; CGCGGGCAAGGTCGACTACAAGGCCAACGAGTGGCTGATGAAAAACATGGACCCTCTGAATGACAACGTC; GCAGCCTTGCTCCACCAGAGCACAGACCGGCTGACGGCAGAGATCTGGAAAGACGTGGAGGGCATCGTGG; GGCTGGAACAGGTGAGCAGCCTGGGCGACGGCCCACCAGGTGGCCGCCCCCGTCGGGGTATGTTCCGGAC; AGTGGGACAGCTCTACAAGGAGTCCCTGAGCCGCCTCATGGCCACACTCAGCAACACCAACCCCAGTTTT; GTCCGCTGCATTGTCCCCAACCACGAGAAGAGGGCCGGGAAGCTGGAGCCACGGCTGGTGCTGGACCAGC; TTCGCTGCAACGGGGTCCTGGAGGGCATCCGCATCTGTCGCCAGGGCTTCCCCAACCGCATCCTCTTCCA; GGAGTTCCGGCAGCGATACGAGATCCTGACACCCAATGCCATCCCCAAGGGCTTCATGGATGGGAAGCAG; GCCTGTGAAAAGATGATCCAGGCGCTGGAACTGGACCCCAACCTCTACCGCGTGGGACAGAGCAAGATCT; TCTTCCGGGCTGGGGTCCTGGCCCAGCTGGAAGAGGAGCGAGACCTGAAGGTCACCGACATCATCGTCTC; CTTCCAGGCAGCTGCCCGGGGATACCTGGCTCGCAGGGCCTTCCAGAAGCGCCAGCAGCAGCAGAGCGCC; CTGAGGGTGATGCAGCGGAACTGCGCGGCCTACCTCAAGCTGAGACACTGGCAGTGGTGGCGGCTGTTTA; CCAAGGTGAAGCCACTGCTGCAGGTGACGCGGCAGGATGAGGTGCTGCAGGCACGGGCCCAGGAGCTGCA; GAAAGTGCAGGAGCTACAGCAGCAGAGCGCCCGCGAAGTTGGGGAGCTCCAGGGCCGAGTGGCACAGCTG; GAAGAGGAGCGCGCCCGCCTGGCAGAGCAATTGCGAGCAGAGGCAGAACTGTGTGCAGAGGCCGAGGAGA; CGCGGGGGAGGCTGGCAGCCCGCAAGCAGGAGCTGGAGCTGGTGGTGTCAGAGCTGGAGGCTCGCGTGGG; CGAGGAGGAGGAGTGCAGCCGTCAAATGCAAACCGAGAAGAAGAGGCTGCAGCAGCACATACAGGAGCTA; GAGGCCCACCTTGAGGCTGAGGAGGGTGCGCGGCAGAAGCTGCAGCTGGAGAAGGTGACGACAGAGGCAA; AAATGAAGAAATTTGAAGAGGACCTGCTGCTCCTGGAAGACCAGAATTCCAAGCTGAGCAAGGAGCGGAA; GCTGCTGGAAGATCGTCTGGCCGAGTTCTCATCCCAGGCAGCTGAGGAGGAGGAGAAGGTCAAGAGCCTC; AATAAGCTACGGCTCAAATATGAGGCCACAATCGCAGACATGGAGGACCGCCTACGGAAGGAGGAGAAGG; GTCGCCAGGAGCTGGAGAAGCTGAAGCGGAGGCTGGATGGGGAGAGCTCAGAGCTGCAGGAGCAGATGGT; GGAGCAGCAACAGCGGGCAGAGGAGCTGCGGGCCCAGCTGGGCCGGAAGGAGGAGGAGCTGCAGGCTGCC; CTGGCCAGGGCAGAAGACGAGGGTGGGGCCCGGGCCCAGCTGCTGAAATCCCTGCGGGAGGCTCAAGCAG; CCCTGGCCGAGGCCCAGGAGGACCTGGAGTCTGAGCGTGTGGCCAGGACCAAGGCGGAGAAGCAGCGCCG; GGACCTGGGCGAGGAGCTGGAGGCGCTGCGGGGCGAGCTGGAGGACACGCTGGACTCCACCAACGCACAG; CAGGAGCTCCGGTCCAAGAGGGAACAGGAGGTGACGGAGCTGAAGAAGACTCTGGAGGAGGAGACTCGCA; TCCACGAGGCGGCAGTGCAGGAGCTGAGGCAGCGCCACGGCCAGGCCCTGGGGGAGCTGGCGGAGCAGCT; GGAGCAGGCCCGGAGGGGCAAAGGTGCATGGGAGAAGACCCGGCTGGCCCTGGAGGCCGAGGTGTCCGAG; CTGCGGGCAGAACTGAGCAGCCTGCAGACTGCACGTCAGGAGGGTGAGCAGCGGAGGCGCCGCCTGGAGT; TACAGCTGCAGGAGGTGCAGGGCCGGGCTGGTGATGGGGAGAGGGCACGAGCGGAGGCTGCTGAGAAGCT; GCAGCGAGCCCAGGCTGAACTGGAGAATGTGTCTGGGGCGCTGAACGAGGCTGAGTCCAAAACCATCCGT; CTTAGCAAGGAGCTGAGCAGCACAGAAGCCCAGCTGCACGATGCCCAGGAGCTGCTGCAGGAGGAGACCA; GGGCGAAATTGGCCTTGGGGTCCCGGGTGCGAGCCATGGAGGCTGAGGCAGCCGGGCTGCGTGAGCAGCT; GGAGGAGGAGGCAGCTGCCAGGGAACGGGCGGGCCGTGAACTGCAGACTGCCCAGGCCCAGCTTTCCGAG; TGGCGGCGGCGCCAGGAGGAGGAGGCAGGGGCACTGGAGGCAGGGGAGGAGGCACGGCGCCGGGCAGCCC; GGGAGGCCGAGGCCCTGACCCAGCGCCTGGCAGAAAAGACAGAGACCGTGGATCGGCTGGAGCGGGGCCG; CCGCCGGCTGCAGCAGGAGCTGGACGACGCCACCATGGACCTGGAGCAGCAGCGGCAGCTTGTGAGCACC; CTGGAGAAGAAGCAGCGCAAGTTTGACCAGCTTCTGGCAGAGGAGAAGGCAGCTGTACTTCGGGCAGTGG; AGGAACGTGAGCGGGCCGAGGCAGAGGGCCGGGAGCGTGAGGCTCGGGCCCTGTCACTGACACGGGCACT; GGAGGAGGAGCAGGAGGCACGTGAGGAGCTGGAGCGGCAGAACCGGGCCCTGCGGGCTGAGCTGGAGGCA; CTGCTGAGCAGCAAGGATGACGTCGGCAAGAGCGTGCATGAGCTGGAACGAGCCTGCCGGGTAGCAGAAC; AGGCAGCCAATGATCTGCGAGCACAGGTGACAGAACTGGAGGATGAGCTGACAGCGGCCGAGGATGCCAA; GCTGCGTCTGGAGGTGACTGTGCAGGCTCTCAAGACTCAGCATGAGCGTGACCTGCAGGGCCGTGATGAG; GCTGGTGAAGAGAGGCGGAGGCAGCTGGCCAAGCAGCTGAGAGATGCAGAGGTGGAGCGGGATGAGGAGC; GGAAGCAGCGCACTCTGGCCGTGGCTGCCCGCAAGAAGCTGGAGGGAGAGCTGGAGGAGCTGAAGGCTCA; GATGGCCTCTGCCGGCCAGGGCAAGGAGGAGGCGGTGAAGCAGCTTCGCAAGATGCAGGCCCAGATGAAG; GAGCTATGGCGGGAGGTGGAGGAGACACGCACCTCCCGGGAGGAGATCTTCTCCCAGAATCGGGAAAGTG; AAAAGCGCCTCAAGGGCCTGGAGGCTGAGGTGCTGCGGCTGCAGGAGGAACTGGCCGCCTCGGACCGTGC; TCGGCGGCAGGCCCAGCAGGACCGGGATGAGATGGCAGATGAGGTGGCCAATGGTAACCTTAGCAAGGCA; GCCATTCTGGAGGAGAAGCGTCAGCTGGAGGGGCGCCTGGGGCAGTTGGAGGAAGAGCTGGAGGAGGAGC; AGAGCAACTCGGAGCTGCTCAATGACCGCTACCGCAAGCTGCTCCTGCAGGTAGAGTCACTGACCACAGA; GCTGTCAGCTGAGCGCAGTTTCTCAGCCAAGGCAGAGAGCGGGCGGCAGCAGCTGGAACGGCAGATCCAG; GAGCTACGGGGACGCCTGGGTGAGGAGGATGCTGGGGCCCGTGCCCGCCACAAGATGACCATTGCTGCCC; TTGAGTCTAAGTTGGCCCAGGCTGAGGAGCAGCTAGAGCAAGAGACCAGAGAGCGCATCCTCTCTGGAAA; GCTGGTGCGCAGAGCTGAGAAGCGGCTTAAAGAGGTGGTGCTCCAGGTGGAGGAGGAGCGGAGGGTGGCT; GACCAGCTCCGGGACCAGCTGGAGAAGGGAAACCTTCGAGTCAAGCAGCTGAAGCGGCAGCTGGAGGAGG; CCGAGGAGGAGGCATCCCGGGCTCAGGCCGGCCGCCGGAGGCTGCAGCGTGAGCTGGAAGATGTCACAGA; GTCGGCCGAGTCCATGAACCGTGAAGTGACCACACTGAGGAACCGGCTTCGACGCGGCCCCCTCACCTTC; ACCACCCGCACGGTGCGCCAGGTCTTCCGACTAGAGGAGGGCGTGGCATCCGACGAGGAGGCAGAGGAAG; CACAGCCTGGGTCTGGGCCATCCCCGGAGCCTGAGGGGTCCCCACCAGCCCACCCCCAG; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC221497 representing NM_001077186; Red=Cloning site Green=Tags(s); ; MAAVTMSVPGRKAPPRPGPVPEAAQPFLFTPRGPSAGGGPGSGTSPQVEWTARRLVWVPSELHGFEAAAL; RDEGEEEAEVELAESGRRLRLPRDQIQRMNPPKFSKAEDMAELTCLNEASVLHNLRERYYSGLIYTYSGL; FCVVINPYKQLPIYTEAIVEMYRGKKRHEVPPHVYAVTEGAYRSMLQDREDQSILCTGESGAGKTENTKK; VIQYLAHVASSPKGRKEPGVPASVSTVSYGELERQLLQANPILEAFGNAKTVKNDNSSRFGKFIRINFDV; AGYIVGANIETYLLEKSRAIRQAKDECSFHIFYQLLGGAGEQLKADLLLEPCSHYRFLTNGPSSSPGQER; ELFQETLESLRVLGFSHEEIISMLRMVSAVLQFGNIALKRERNTDQATMPDNTAAQKLCRLLGLGVTDFS; RALLTPRIKVGRDYVQKAQTKEQADFALEALAKATYERLFRWLVLRLNRALDRSPRQGASFLGILDIAGF; EIFQLNSFEQLCINYTNEKLQQLFNHTMFVLEQEEYQREGIPWTFLDFGLDLQPCIDLIERPANPPGLLA; LLDEECWFPKATDKSFVEKVAQEQGGHPKFQRPRHLRDQADFSVLHYAGKVDYKANEWLMKNMDPLNDNV; AALLHQSTDRLTAEIWKDVEGIVGLEQVSSLGDGPPGGRPRRGMFRTVGQLYKESLSRLMATLSNTNPSF; VRCIVPNHEKRAGKLEPRLVLDQLRCNGVLEGIRICRQGFPNRILFQEFRQRYEILTPNAIPKGFMDGKQ; ACEKMIQALELDPNLYRVGQSKIFFRAGVLAQLEEERDLKVTDIIVSFQAAARGYLARRAFQKRQQQQSA; LRVMQRNCAAYLKLRHWQWWRLFTKVKPLLQVTRQDEVLQARAQELQKVQELQQQSAREVGELQGRVAQL; EEERARLAEQLRAEAELCAEAEETRGRLAARKQELELVVSELEARVGEEEECSRQMQTEKKRLQQHIQEL; EAHLEAEEGARQKLQLEKVTTEAKMKKFEEDLLLLEDQNSKLSKERKLLEDRLAEFSSQAAEEEEKVKSL; NKLRLKYEATIADMEDRLRKEEKGRQELEKLKRRLDGESSELQEQMVEQQQRAEELRAQLGRKEEELQAA; LARAEDEGGARAQLLKSLREAQAALAEAQEDLESERVARTKAEKQRRDLGEELEALRGELEDTLDSTNAQ; QELRSKREQEVTELKKTLEEETRIHEAAVQELRQRHGQALGELAEQLEQARRGKGAWEKTRLALEAEVSE; LRAELSSLQTARQEGEQRRRRLELQLQEVQGRAGDGERARAEAAEKLQRAQAELENVSGALNEAESKTIR; LSKELSSTEAQLHDAQELLQEETRAKLALGSRVRAMEAEAAGLREQLEEEAAARERAGRELQTAQAQLSE; WRRRQEEEAGALEAGEEARRRAAREAEALTQRLAEKTETVDRLERGRRRLQQELDDATMDLEQQRQLVST; LEKKQRKFDQLLAEEKAAVLRAVEERERAEAEGREREARALSLTRALEEEQEAREELERQNRALRAELEA; LLSSKDDVGKSVHELERACRVAEQAANDLRAQVTELEDELTAAEDAKLRLEVTVQALKTQHERDLQGRDE; AGEERRRQLAKQLRDAEVERDEERKQRTLAVAARKKLEGELEELKAQMASAGQGKEEAVKQLRKMQAQMK; ELWREVEETRTSREEIFSQNRESEKRLKGLEAEVLRLQEELAASDRARRQAQQDRDEMADEVANGNLSKA; AILEEKRQLEGRLGQLEEELEEEQSNSELLNDRYRKLLLQVESLTTELSAERSFSAKAESGRQQLERQIQ; ELRGRLGEEDAGARARHKMTIAALESKLAQAEEQLEQETRERILSGKLVRRAEKRLKEVVLQVEEERRVA; DQLRDQLEKGNLRVKQLKRQLEEAEEEASRAQAGRRRLQRELEDVTESAESMNREVTTLRNRLRRGPLTF; TTRTVRQVFRLEEGVASDEEAEEAQPGSGPSPEPEGSPPAHPQ; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001077186
• ORF Size: 6009 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001077186.2
• RefSeq Size: 6831 bp
• RefSeq ORF: 6012 bp
• Locus ID: 79784
• UniProt ID: Q7Z406
• Cytogenetics: 19q13.33
• Protein Pathways: Regulation of actin cytoskeleton, Tight junction, Viral myocarditis
• MW: 228.5 kDa
• Summary: This gene encodes a member of the myosin superfamily. The protein represents a conventional non-muscle myosin; it should not be confused with the unconventional myosin-14 (MYO14). Myosins are actin-dependent motor proteins with diverse functions including regulation of cytokinesis, cell motility, and cell polarity. Mutations in this gene result in one form of autosomal dominant hearing impairment. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011]