EAAT4 (SLC1A6) (NM_005071) Human Tagged ORF Clone

SKU RC221087

SLC1A6 (Myc-DDK-tagged)-Human solute carrier family 1 (high affinity aspartate/glutamate transporter), member 6 (SLC1A6)

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: EAAT4
• Synonyms: EAAT4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC221087 representing NM_005071; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGAGCAGCCATGGCAACAGCCTGTTCCTTCGGGAGAGCGGCCAGCGGCTGGGCCGGGTGGGCTGGCTGC; AGCGGCTGCAGGAAAGCCTGCAGCAGAGAGCACTGCGCACGCGCCTGCGCCTGCAGACCATGACCCTCGA; GCACGTGCTGCGCTTCCTGCGCCGAAACGCCTTCATTCTGCTGACGGTCAGCGCCGTGGTCATTGGGGTC; AGCCTGGCCTTTGCCCTGCGCCCATATCAGCTCACCTACCGCCAGATCAAGTACTTCTCTTTTCCTGGAG; AGCTTCTGATGAGGATGCTGCAGATGCTGGTGTTACCTCTCATTGTCTCCAGCCTGGTCACAGGTATGGC; ATCCCTGGACAACAAGGCCACGGGGCGGATGGGGATGCGGGCAGCTGTGTACTACATGGTGACCACCATC; ATCGCGGTCTTCATCGGCATCCTCATGGTCACCATCATCCATCCCGGGAAGGGCTCCAAGGAGGGGCTGC; ACCGGGAGGGCCGGATCGAGACCATCCCCACAGCTGATGCCTTCATGGACCTGATCAGAAATATGTTTCC; ACCAAACCTTGTGGAGGCCTGCTTCAAACAGTTCAAGACGCAGTACAGCACGAGGGTGGTAACCAGGACC; ATGGTGAGGACAGAGAACGGGTCTGAGCCGGGTGCCTCCATGCCTCCTCCATTCTCAGTGGAGAACGGAA; CCAGCTTCCTGGAAAATGTCACTCGGGCCTTGGGTACCCTGCAGGAGATGCTGAGCTTTGAGGAGACTGT; ACCCGTGCCTGGCTCCGCCAATGGCATCAACGCCCTGGGCCTCGTGGTCTTCTCTGTGGCCTTTGGGCTG; GTCATTGGTGGCATGAAACACAAGGGCAGAGTCCTCAGGGACTTCTTCGACAGCCTCAATGAGGCTATTA; TGAGGCTGGTGGGCATCATTATCTGGTATGCACCTGTGGGCATCCTGTTCCTGATTGCTGGGAAGATTCT; GGAGATGGAAGACATGGCCGTCCTGGGGGGTCAGCTGGGCATGTACACCCTGACCGTCATCGTGGGCCTG; TTCCTCCATGCCGGCATTGTCCTTCCCCTCATCTACTTCCTCGTCACTCACCGGAACCCCTTCCCCTTCA; TTGGGGGCATGCTACAAGCCCTCATCACCGCTATGGGCACGTCTTCCAGCTCGGCAACGCTGCCCATCAC; CTTCCGCTGCCTGGAGGAGGGCCTGGGTGTGGACCGCCGCATCACCAGGTTCGTCCTGCCCGTGGGCGCC; ACGGTCAACATGGATGGCACTGCCCTCTACGAGGCCCTGGCTGCCATCTTCATTGCTCAAGTTAACAACT; ACGAGCTCAACCTGGGTCAGATCACAACCATCAGCATCACGGCCACAGCAGCCAGTGTTGGGGCTGCTGG; CATCCCCCAGGCGGGTCTGGTCACCATGGTCATTGTGCTTACGTCGGTCGGCTTGCCCACGGAAGACATC; ACGCTCATCATTGCCGTGGACTGGTTCCTTGACCGGCTTCGCACAATGACCAACGTACTGGGGGACTCAA; TTGGAGCGGCCGTCATCGAGCACTTGTCTCAGCGGGAGCTGGAGCTTCAGGAAGCTGAGCTTACCCTCCC; CAGCCTGGGGAAACCCTACAAGTCCCTCATGGCACAGGAGAAGGGGGCATCCCGGGGACGGGGAGGCAAC; GAGAGTGCTATG; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC221087 representing NM_005071; Red=Cloning site Green=Tags(s); ; MSSHGNSLFLRESGQRLGRVGWLQRLQESLQQRALRTRLRLQTMTLEHVLRFLRRNAFILLTVSAVVIGV; SLAFALRPYQLTYRQIKYFSFPGELLMRMLQMLVLPLIVSSLVTGMASLDNKATGRMGMRAAVYYMVTTI; IAVFIGILMVTIIHPGKGSKEGLHREGRIETIPTADAFMDLIRNMFPPNLVEACFKQFKTQYSTRVVTRT; MVRTENGSEPGASMPPPFSVENGTSFLENVTRALGTLQEMLSFEETVPVPGSANGINALGLVVFSVAFGL; VIGGMKHKGRVLRDFFDSLNEAIMRLVGIIIWYAPVGILFLIAGKILEMEDMAVLGGQLGMYTLTVIVGL; FLHAGIVLPLIYFLVTHRNPFPFIGGMLQALITAMGTSSSSATLPITFRCLEEGLGVDRRITRFVLPVGA; TVNMDGTALYEALAAIFIAQVNNYELNLGQITTISITATAASVGAAGIPQAGLVTMVIVLTSVGLPTEDI; TLIIAVDWFLDRLRTMTNVLGDSIGAAVIEHLSQRELELQEAELTLPSLGKPYKSLMAQEKGASRGRGGN; ESAM; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Chromatograms: Chromatograms ; Sequencher program is needed, download here
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005071
• ORF Size: 1692 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_005071.2
• RefSeq Size: 1719 bp
• RefSeq ORF: 1695 bp
• Locus ID: 6511
• UniProt ID: P48664
• Cytogenetics: 19p13.12
• Domains: SDF
• Protein Families: Transmembrane
• MW: 61.4 kDa
• Summary: Sodium-dependent, high-affinity amino acid transporter that mediates the uptake of L-glutamate and also L-aspartate and D-aspartate (PubMed:7791878). Functions as a symporter that transports one amino acid molecule together with two or three Na(+) ions and one proton, in parallel with the counter-transport of one K(+) ion. Mediates Cl(-) flux that is not coupled to amino acid transport; this avoids the accumulation of negative charges due to aspartate and Na(+) symport (By similarity). Plays a redundant role in the rapid removal of released glutamate from the synaptic cleft, which is essential for terminating the postsynaptic action of glutamate (Probable).[UniProtKB/Swiss-Prot Function]