Cytochrome P450 26B (CYP26B1) (NM_019885) Human Tagged ORF Clone

SKU RC221075

CYP26B1 (Myc-DDK-tagged)-Human cytochrome P450, family 26, subfamily B, polypeptide 1 (CYP26B1)

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: Cytochrome P450 26B
• Synonyms: CYP26A2; P450RAI-2; P450RAI2; RHFCA
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC221075 representing NM_019885; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGCTCTTTGAGGGCTTGGATCTGGTGTCGGCGCTGGCCACCCTCGCCGCGTGCCTGGTGTCCGTGACGC; TGCTGCTGGCCGTGTCGCAGCAGCTGTGGCAGCTGCGCTGGGCCGCCACTCGCGACAAGAGCTGCAAGCT; GCCCATCCCCAAGGGATCCATGGGCTTCCCGCTCATCGGAGAGACCGGCCGCTGGCTGCTGCAGGGTTCT; GGCTTCCAGTCGTCGCGGAGGGAGAAGTATGGCAACGTGTTCAAGACGCATTTGTTGGGGCGGCCGCTGA; TACGCGTGACCGGCGCGGAGAACGTGCGCAAGATCCTCATGGGCGAGCACCACCTCGTGAGCACCGAGTG; GCCTCGCAGCACCCGCATGTTGCTGGGCCCCAACACGGTGTCCAATTCCATTGGCGACATCCACCGCAAC; AAGCGCAAGGTCTTCTCCAAGATCTTCAGCCACGAGGCCCTGGAGAGTTACCTGCCCAAGATCCAGCTGG; TGATCCAGGACACACTGCGCGCCTGGAGCAGCCACCCCGAGGCCATCAACGTGTACCAGGAGGCGCAGAA; GCTGACCTTCCGCATGGCCATCCGGGTGCTGCTGGGCTTCAGCATCCCTGAGGAGGACCTTGGGCACCTC; TTTGAGGTCTACCAGCAGTTTGTGGACAATGTCTTCTCCCTGCCTGTCGACCTGCCCTTCAGTGGCTACC; GGCGGGGCATTCAGGCTCGGCAGATCCTGCAGAAGGGGCTGGAGAAGGCCATCCGGGAGAAGCTGCAGTG; CACACAGAGCAAGGACTACTTGGACGCCCTGGACCTCCTCATTGAGAGCAGCAAGGAGCACGGGAAGGAG; ATGACCATGCAGGAGCTGAAGGACGGGACCCTGGAGCTGATCTTTGCGGCCTATGCCACCACGGCCAGCG; CCAGCACCTCACTCATCATGCAGCTGCTGAAGCACCCCACTGTGCTGGAGAAGCTGCGGGATGAGCTGCG; GGCTCATGGCATCCTGCACAGTGGCGGCTGCCCCTGCGAGGGCACACTGCGCCTGGACACGCTCAGTGGG; CTGCGCTACCTGGACTGCGTCATCAAGGAGGTCATGCGCCTGTTCACGCCCATTTCCGGCGGCTACCGCA; CTGTGCTGCAGACCTTCGAGCTTGATGGTTTCCAGATCCCCAAAGGCTGGAGTGTCATGTATAGCATCCG; GGACACCCATGACACAGCGCCCGTGTTCAAAGACGTGAACGTGTTCGACCCCGATCGCTTCAGCCAGGCG; CGGAGCGAGGACAAGGATGGCCGCTTCCATTACCTCCCGTTCGGTGGCGGTGTCCGGACCTGCCTGGGCA; AGCACCTGGCCAAGCTGTTCCTGAAGGTGCTGGCGGTGGAGCTGGCTAGCACCAGCCGCTTTGAGCTGGC; CACACGGACCTTCCCCCGCATCACCTTGGTCCCCGTCCTGCACCCCGTGGATGGCCTCAGCGTCAAGTTC; TTTGGCCTGGACTCCAACCAGAACGAGATCCTGCCGGAGACGGAGGCCATGCTGAGCGCCACAGTC; ; AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC; TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC221075 representing NM_019885; Red=Cloning site Green=Tags(s); ; MLFEGLDLVSALATLAACLVSVTLLLAVSQQLWQLRWAATRDKSCKLPIPKGSMGFPLIGETGRWLLQGS; GFQSSRREKYGNVFKTHLLGRPLIRVTGAENVRKILMGEHHLVSTEWPRSTRMLLGPNTVSNSIGDIHRN; KRKVFSKIFSHEALESYLPKIQLVIQDTLRAWSSHPEAINVYQEAQKLTFRMAIRVLLGFSIPEEDLGHL; FEVYQQFVDNVFSLPVDLPFSGYRRGIQARQILQKGLEKAIREKLQCTQSKDYLDALDLLIESSKEHGKE; MTMQELKDGTLELIFAAYATTASASTSLIMQLLKHPTVLEKLRDELRAHGILHSGGCPCEGTLRLDTLSG; LRYLDCVIKEVMRLFTPISGGYRTVLQTFELDGFQIPKGWSVMYSIRDTHDTAPVFKDVNVFDPDRFSQA; RSEDKDGRFHYLPFGGGVRTCLGKHLAKLFLKVLAVELASTSRFELATRTFPRITLVPVLHPVDGLSVKF; FGLDSNQNEILPETEAMLSATV; ; SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_019885
• ORF Size: 1536 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_019885.3, NP_063938.1
• RefSeq Size: 4567 bp
• RefSeq ORF: 1539 bp
• Locus ID: 56603
• UniProt ID: Q9NR63
• Cytogenetics: 2p13.2
• Protein Families: Druggable Genome, P450
• Protein Pathways: Retinol metabolism
• MW: 58 kDa
• Summary: This gene encodes a member of the cytochrome P450 superfamily. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. The encoded protein is localized to the endoplasmic reticulum, and functions as a critical regulator of all-trans retinoic acid levels by the specific inactivation of all-trans retinoic acid to hydroxylated forms. Mutations in this gene are associated with radiohumeral fusions and other skeletal and craniofacial anomalies, and increased levels of the encoded protein are associated with atherosclerotic lesions. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2013]