Cytochrome P450 2C9 (CYP2C9) (NM_000771) Human Tagged Lenti ORF Clone

SKU
RC220997L3
Lenti ORF clone of Human cytochrome P450, family 2, subfamily C, polypeptide 9 (CYP2C9), Myc-DDK-tagged
  • LentiORF®
    LentiORF®

    Expression-ready ORF plasmid in lenti backbone

    Click here to learn more.

$986.00
3 Weeks*
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol Cytochrome P450 2C9
Synonyms CPC9; CYP2C; CYP2C10; CYPIIC9; P450IIC9
Vector pLenti-C-Myc-DDK-P2A-Puro
E. coli Selection Chl (34 ug/mL)
Mammalian Cell Selection Puromycin
Sequence Data
ORF Nucleotide Sequence
The ORF insert of this clone is exactly the same as(RC220997).
Restriction Sites SgfI-MluI Cloning Scheme for this gene Plasmid Map
ACCN NM_000771
ORF Size 1470 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_000771.2
RefSeq Size 1835 bp
RefSeq ORF 1473 bp
Locus ID 1559
UniProt ID P11712
Cytogenetics 10q23.33
Domains p450
Protein Families Druggable Genome, P450
Protein Pathways Arachidonic acid metabolism, Drug metabolism - cytochrome P450, Linoleic acid metabolism, Metabolic pathways, Metabolism of xenobiotics by cytochrome P450, Retinol metabolism
MW 55.4 kDa
Summary This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and its expression is induced by rifampin. The enzyme is known to metabolize many xenobiotics, including phenytoin, tolbutamide, ibuprofen and S-warfarin. Studies identifying individuals who are poor metabolizers of phenytoin and tolbutamide suggest that this gene is polymorphic. The gene is located within a cluster of cytochrome P450 genes on chromosome 10q24. [provided by RefSeq, Jul 2008]
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SKU Description Size Price
RC220997 CYP2C9 (Myc-DDK-tagged)-Human cytochrome P450, family 2, subfamily C, polypeptide 9 (CYP2C9) 10 ug
$686.00
RC220997L1 Lenti ORF clone of Human cytochrome P450, family 2, subfamily C, polypeptide 9 (CYP2C9), Myc-DDK-tagged 10 ug
$986.00
RC220997L2 Lenti ORF clone of Human cytochrome P450, family 2, subfamily C, polypeptide 9 (CYP2C9), mGFP tagged 10 ug
$986.00
RC220997L4 Lenti ORF clone of Human cytochrome P450, family 2, subfamily C, polypeptide 9 (CYP2C9), mGFP tagged 10 ug
$986.00
RG220997 CYP2C9 (tGFP-tagged) - Human cytochrome P450, family 2, subfamily C, polypeptide 9 (CYP2C9) 10 ug
$886.00
SC128275 CYP2C9 (untagged)-Human cytochrome P450, family 2, subfamily C, polypeptide 9 (CYP2C9) 10 ug
$686.00

Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.