PKA R2 (PRKAR2A) (NM_004157) Human Tagged ORF Clone

SKU RC220376

PRKAR2A (Myc-DDK-tagged)-Human protein kinase, cAMP-dependent, regulatory, type II, alpha (PRKAR2A)

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: PKA R2
• Synonyms: PKR2; PRKAR2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC220376 representing NM_004157; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGAGCCACATCCAGATCCCGCCGGGGCTCACGGAGCTGCTGCAGGGCTACACGGTGGAGGTGCTGCGAC; AGCAGCCGCCTGACCTCGTCGAATTCGCAGTGGAGTACTTCACCCGCCTGCGCGAGGCCCGCGCCCCAGC; CTCAGTCCTGCCCGCCGCCACCCCACGCCAGAGCCTGGGCCACCCCCCGCCAGAACCCGGCCCGGACCGT; GTCGCCGACGCCAAAGGGGACAGCGAGTCGGAGGAGGACGAGGACTTGGAAGTTCCAGTTCCTAGCAGAT; TTAATAGACGAGTATCAGTCTGTGCTGAGACCTATAACCCTGATGAGGAAGAGGAAGATACAGATCCAAG; GGTGATTCATCCTAAAACTGATGAACAGAGATGCAGACTTCAGGAAGCTTGCAAAGATATTCTCCTTTTC; AAAAATCTTGATCAGGAACAGCTTTCTCAAGTTCTCGATGCCATGTTTGAAAGGATAGTCAAAGCTGATG; AGCATGTCATTGACCAAGGAGATGATGGAGACAACTTTTATGTCATAGAACGGGGAACTTATGACATTTT; AGTAACAAAAGATAATCAAACCCGCTCTGTTGGTCAATATGACAACCGTGGCAGTTTTGGAGAACTAGCT; CTGATGTACAACACCCCGAGAGCTGCTACCATTGTTGCTACCTCAGAAGGCTCCCTTTGGGGACTGGACC; GGGTGACTTTTAGAAGAATCATAGTGAAAAATAATGCAAAGAAGAGGAAGATGTTTGAATCATTTATTGA; GTCTGTGCCCCTCCTTAAATCACTAGAGGTGTCAGAACGAATGAAGATTGTGGATGTAATAGGAGAGAAG; ATCTATAAGGATGGAGAACGCATAATCACTCAGGGTGAAAAGGCTGATAGCTTTTACATCATAGAGTCTG; GCGAAGTGAGCATCTTGATTAGAAGCAGGACTAAATCAAACAAGGATGGTGGGAACCAGGAGGTCGAGAT; TGCCCGCTGCCATAAGGGGCAGTACTTTGGAGAGCTTGCCCTGGTCACCAACAAACCCAGAGCTGCCTCA; GCTTATGCAGTTGGAGATGTCAAATGCTTAGTTATGGATGTACAAGCATTCGAGAGGCTTCTGGGGCCCT; GCATGGACATCATGAAGAGGAACATCTCACACTATGAGGAACAGCTGGTGAAGATGTTTGGCTCCAGCGT; GGATCTGGGCAACCTCGGGCAG; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC220376 representing NM_004157; Red=Cloning site Green=Tags(s); ; MSHIQIPPGLTELLQGYTVEVLRQQPPDLVEFAVEYFTRLREARAPASVLPAATPRQSLGHPPPEPGPDR; VADAKGDSESEEDEDLEVPVPSRFNRRVSVCAETYNPDEEEEDTDPRVIHPKTDEQRCRLQEACKDILLF; KNLDQEQLSQVLDAMFERIVKADEHVIDQGDDGDNFYVIERGTYDILVTKDNQTRSVGQYDNRGSFGELA; LMYNTPRAATIVATSEGSLWGLDRVTFRRIIVKNNAKKRKMFESFIESVPLLKSLEVSERMKIVDVIGEK; IYKDGERIITQGEKADSFYIIESGEVSILIRSRTKSNKDGGNQEVEIARCHKGQYFGELALVTNKPRAAS; AYAVGDVKCLVMDVQAFERLLGPCMDIMKRNISHYEEQLVKMFGSSVDLGNLGQ; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Chromatograms: Chromatograms ; Sequencher program is needed, download here
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004157
• ORF Size: 1212 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_004157.4
• RefSeq Size: 2381 bp
• RefSeq ORF: 1215 bp
• Locus ID: 5576
• UniProt ID: P13861
• Cytogenetics: 3p21.31
• Domains: cNMP, RIIa
• Protein Families: Druggable Genome
• Protein Pathways: Apoptosis, Insulin signaling pathway
• MW: 45.3 kDa
• Summary: cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. The protein encoded by this gene is one of the regulatory subunits. This subunit can be phosphorylated by the activated catalytic subunit. It may interact with various A-kinase anchoring proteins and determine the subcellular localization of cAMP-dependent protein kinase. This subunit has been shown to regulate protein transport from endosomes to the Golgi apparatus and further to the endoplasmic reticulum (ER). [provided by RefSeq, Jul 2008]