Xanthine Oxidase (XDH) (NM_000379) Human Tagged ORF Clone

SKU RC219402

XDH (Myc-DDK-tagged)-Human xanthine dehydrogenase (XDH)

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: Xanthine Oxidase
• Synonyms: XAN1; XO; XOR
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC219402 representing NM_000379; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGACAGCAGACAAATTGGTTTTCTTTGTGAATGGCAGAAAGGTGGTGGAGAAAAATGCAGATCCAGAGA; CAACCCTTTTGGCCTACCTGAGAAGAAAGTTGGGGCTGAGTGGAACCAAGCTCGGCTGTGGAGAGGGGGG; CTGCGGGGCTTGCACAGTGATGCTCTCCAAGTATGATCGTCTGCAGAACAAGATCGTCCACTTTTCTGCC; AATGCCTGCCTGGCCCCCATCTGCTCCTTGCACCATGTTGCAGTGACAACTGTGGAAGGAATAGGAAGCA; CCAAGACGAGGCTGCATCCTGTGCAGGAGAGAATTGCCAAAAGCCACGGCTCCCAGTGCGGGTTCTGCAC; CCCTGGCATCGTCATGAGTATGTACACACTGCTCCGGAATCAGCCCGAGCCCACCATGGAGGAGATTGAG; AATGCCTTCCAAGGAAATCTGTGCCGCTGCACAGGCTACAGACCCATCCTCCAGGGCTTCCGGACCTTTG; CCAGGGATGGTGGATGCTGTGGAGGAGATGGGAATAATCCAAATTGCTGCATGAACCAGAAGAAAGACCA; CTCAGTCAGCCTCTCGCCATCTTTATTCAAACCAGAGGAGTTCACGCCCCTGGATCCAACCCAGGAGCCC; ATTTTTCCCCCAGAGTTGCTGAGGCTGAAAGACACTCCTCGGAAGCAGCTGCGATTTGAAGGGGAGCGTG; TGACGTGGATACAGGCCTCAACCCTCAAGGAGCTGCTGGACCTCAAGGCTCAGCACCCTGACGCCAAGCT; GGTCGTGGGGAACACGGAGATTGGCATTGAGATGAAGTTCAAGAATATGCTGTTTCCTATGATTGTCTGC; CCAGCCTGGATCCCTGAGCTGAATTCGGTAGAACATGGACCCGACGGTATCTCCTTTGGAGCTGCTTGCC; CCCTGAGCATTGTGGAAAAAACCCTGGTGGATGCTGTTGCTAAGCTTCCTGCCCAAAAGACAGAGGTGTT; CAGAGGGGTCCTGGAGCAGCTGCGCTGGTTTGCTGGGAAGCAAGTCAAGTCTGTGGCGTCCGTTGGAGGG; AACATCATCACTGCCAGCCCCATCTCCGACCTCAACCCCGTGTTCATGGCCAGTGGGGCCAAGCTGACAC; TTGTGTCCAGAGGCACCAGGAGAACTGTCCAGATGGACCACACCTTCTTCCCTGGCTACAGAAAGACCCT; GCTGAGCCCGGAGGAGATACTGCTCTCCATAGAGATCCCCTACAGCAGGGAGGGGGAGTATTTCTCAGCA; TTCAAGCAGGCCTCCCGGAGAGAAGATGACATTGCCAAGGTAACCAGTGGCATGAGAGTTTTATTCAAGC; CAGGAACCACAGAGGTACAGGAGCTGGCCCTTTGCTATGGTGGAATGGCCAACAGAACCATCTCAGCCCT; CAAGACCACTCAGAGGCAGCTTTCCAAGCTCTGGAAGGAGGAGCTGCTGCAGGACGTGTGTGCAGGACTG; GCAGAGGAGCTGCATCTGCCTCCCGATGCCCCTGGTGGCATGGTGGACTTCCGGTGCACCCTCACCCTCA; GCTTCTTCTTCAAGTTCTACCTGACAGTCCTTCAGAAGCTGGGCCAAGAGAACCTGGAAGACAAGTGTGG; TAAACTGGACCCCACTTTCGCCAGTGCAACTTTACTGTTTCAGAAAGACCCCCCAGCCGATGTCCAGCTC; TTCCAAGAGGTGCCCAAGGGTCAGTCTGAGGAGGACATGGTGGGCCGGCCCCTGCCCCACCTGGCAGCGG; ACATGCAGGCCTCTGGTGAGGCCGTGTACTGTGACGACATTCCTCGCTACGAGAATGAGCTGTCTCTCCG; GCTGGTCACCAGCACCCGGGCCCACGCCAAGATCAAGTCCATAGATACATCAGAAGCTAAGAAGGTTCCA; GGGTTTGTTTGTTTCATTTCCGCTGATGATGTTCCTGGGAGTAACATAACTGGAATTTGTAATGATGAGA; CAGTCTTTGCGAAGGATAAGGTTACTTGTGTTGGGCATATCATTGGTGCTGTGGTTGCTGACACCCCGGA; ACACACACAGAGAGCTGCCCAAGGGGTGAAAATCACCTATGAAGAACTACCAGCCATTATCACAATTGAG; GATGCTATAAAGAACAACTCCTTTTATGGACCTGAGCTGAAGATCGAGAAAGGGGACCTAAAGAAGGGGT; TTTCCGAAGCAGATAATGTTGTGTCAGGGGAGATATACATCGGTGGCCAAGAGCACTTCTACCTGGAGAC; TCACTGCACCATTGCTGTTCCAAAAGGCGAGGCAGGGGAGATGGAGCTCTTTGTGTCTACACAGAACACC; ATGAAGACCCAGAGCTTTGTTGCAAAAATGTTGGGGGTTCCAGCAAACCGGATTGTGGTTCGAGTGAAGA; GAATGGGAGGAGGCTTTGGAGGCAAGGAGACCCGGAGCACTGTGGTGTCCACGGCAGTGGCCCTGGCTGC; ATATAAGACCGGCCGCCCTGTGCGATGCATGCTGGACCGTGATGAGGACATGCTGATAACTGGTGGCAGA; CATCCCTTCCTGGCCAGATACAAGGTTGGCTTCATGAAGACTGGGACAGTTGTGGCTCTTGAGGTGGACC; ACTTCAGCAATGTGGGGAACACCCAGGATCTCTCTCAGAGTATTATGGAACGAGCTTTATTCCACATGGA; CAACTGCTATAAAATCCCCAACATCCGGGGCACTGGGCGGCTGTGCAAAACCAACCTTCCCTCCAACACG; GCCTTCCGGGGCTTTGGGGGGCCCCAGGGGATGCTCATTGCCGAGTGCTGGATGAGTGAAGTTGCAGTGA; CCTGTGGGATGCCTGCAGAGGAGGTGCGGAGAAAAAACCTGTACAAAGAAGGGGACCTGACACACTTCAA; CCAGAAGCTTGAGGGTTTCACCTTGCCCAGATGCTGGGAAGAATGCCTAGCAAGCTCTCAGTATCATGCT; CGGAAGAGTGAGGTTGACAAGTTCAACAAGGAGAATTGTTGGAAAAAGAGAGGATTGTGCATAATTCCCA; CCAAGTTTGGAATAAGCTTCACAGTTCCTTTTCTGAATCAGGCAGGAGCCCTACTTCATGTGTACACAGA; TGGCTCTGTGCTGCTGACCCACGGGGGGACTGAGATGGGCCAAGGCCTTCATACCAAAATGGTCCAGGTG; GCCAGTAGAGCTCTGAAAATCCCCACCTCTAAGATTTATATCAGCGAGACAAGCACTAACACTGTGCCCA; ACACCTCTCCCACGGCTGCCTCTGTCAGCGCTGACCTCAATGGACAGGCCGTCTATGCGGCTTGTCAGAC; CATCTTGAAAAGGCTGGAACCCTACAAGAAGAAGAATCCCAGTGGCTCCTGGGAAGACTGGGTCACAGCT; GCCTACATGGACACAGTGAGCTTGTCTGCCACTGGGTTTTATAGAACACCCAATCTGGGCTACAGCTTTG; AGACTAACTCAGGGAACCCCTTCCACTACTTCAGCTATGGGGTGGCTTGCTCTGAAGTAGAAATCGACTG; CCTAACAGGAGATCATAAGAACCTCCGCACAGATATTGTCATGGATGTTGGCTCCAGTCTAAACCCTGCT; ATTGATATTGGACAGGTGGAAGGGGCATTTGTCCAGGGCCTTGGCCTCTTCACCCTAGAGGAGCTACACT; ATTCCCCCGAGGGGAGCCTGCACACCCGTGGCCCTAGCACCTACAAGATCCCGGCATTTGGCAGCATCCC; CATTGAGTTCAGGGTGTCCCTGCTCCGCGACTGCCCCAACAAGAAGGCCATCTATGCATCGAAGGCTGTT; GGAGAGCCGCCCCTCTTCCTGGCTGCTTCTATCTTCTTTGCCATCAAAGATGCCATCCGTGCAGCTCGAG; CTCAGCACACAGGTAATAACGTGAAGGAACTCTTCCGGCTAGACAGCCCTGCCACCCCGGAGAAGATCCG; CAATGCCTGCGTGGACAAGTTCACCACCCTGTGTGTCACTGGTGTCCCAGAAAACTGCAAACCCTGGTCT; GTGAGGGTC; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC219402 representing NM_000379; Red=Cloning site Green=Tags(s); ; MTADKLVFFVNGRKVVEKNADPETTLLAYLRRKLGLSGTKLGCGEGGCGACTVMLSKYDRLQNKIVHFSA; NACLAPICSLHHVAVTTVEGIGSTKTRLHPVQERIAKSHGSQCGFCTPGIVMSMYTLLRNQPEPTMEEIE; NAFQGNLCRCTGYRPILQGFRTFARDGGCCGGDGNNPNCCMNQKKDHSVSLSPSLFKPEEFTPLDPTQEP; IFPPELLRLKDTPRKQLRFEGERVTWIQASTLKELLDLKAQHPDAKLVVGNTEIGIEMKFKNMLFPMIVC; PAWIPELNSVEHGPDGISFGAACPLSIVEKTLVDAVAKLPAQKTEVFRGVLEQLRWFAGKQVKSVASVGG; NIITASPISDLNPVFMASGAKLTLVSRGTRRTVQMDHTFFPGYRKTLLSPEEILLSIEIPYSREGEYFSA; FKQASRREDDIAKVTSGMRVLFKPGTTEVQELALCYGGMANRTISALKTTQRQLSKLWKEELLQDVCAGL; AEELHLPPDAPGGMVDFRCTLTLSFFFKFYLTVLQKLGQENLEDKCGKLDPTFASATLLFQKDPPADVQL; FQEVPKGQSEEDMVGRPLPHLAADMQASGEAVYCDDIPRYENELSLRLVTSTRAHAKIKSIDTSEAKKVP; GFVCFISADDVPGSNITGICNDETVFAKDKVTCVGHIIGAVVADTPEHTQRAAQGVKITYEELPAIITIE; DAIKNNSFYGPELKIEKGDLKKGFSEADNVVSGEIYIGGQEHFYLETHCTIAVPKGEAGEMELFVSTQNT; MKTQSFVAKMLGVPANRIVVRVKRMGGGFGGKETRSTVVSTAVALAAYKTGRPVRCMLDRDEDMLITGGR; HPFLARYKVGFMKTGTVVALEVDHFSNVGNTQDLSQSIMERALFHMDNCYKIPNIRGTGRLCKTNLPSNT; AFRGFGGPQGMLIAECWMSEVAVTCGMPAEEVRRKNLYKEGDLTHFNQKLEGFTLPRCWEECLASSQYHA; RKSEVDKFNKENCWKKRGLCIIPTKFGISFTVPFLNQAGALLHVYTDGSVLLTHGGTEMGQGLHTKMVQV; ASRALKIPTSKIYISETSTNTVPNTSPTAASVSADLNGQAVYAACQTILKRLEPYKKKNPSGSWEDWVTA; AYMDTVSLSATGFYRTPNLGYSFETNSGNPFHYFSYGVACSEVEIDCLTGDHKNLRTDIVMDVGSSLNPA; IDIGQVEGAFVQGLGLFTLEELHYSPEGSLHTRGPSTYKIPAFGSIPIEFRVSLLRDCPNKKAIYASKAV; GEPPLFLAASIFFAIKDAIRAARAQHTGNNVKELFRLDSPATPEKIRNACVDKFTTLCVTGVPENCKPWS; VRV; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Chromatograms: Chromatograms ; Sequencher program is needed, download here
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_000379
• ORF Size: 3999 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_000379.4
• RefSeq Size: 4428 bp
• RefSeq ORF: 4002 bp
• Locus ID: 7498
• UniProt ID: P47989
• Cytogenetics: 2p23.1
• Protein Families: Druggable Genome
• Protein Pathways: Caffeine metabolism, Drug metabolism - other enzymes, Metabolic pathways, Purine metabolism
• MW: 146.2 kDa
• Summary: Xanthine dehydrogenase belongs to the group of molybdenum-containing hydroxylases involved in the oxidative metabolism of purines. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Xanthine dehydrogenase can be converted to xanthine oxidase by reversible sulfhydryl oxidation or by irreversible proteolytic modification. Defects in xanthine dehydrogenase cause xanthinuria, may contribute to adult respiratory stress syndrome, and may potentiate influenza infection through an oxygen metabolite-dependent mechanism. [provided by RefSeq, Jan 2014]