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CD47 (NM_001777) Human Tagged ORF Clone

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SKU
RC218813
CD47 (Myc-DDK-tagged)-Human CD47 molecule (CD47), transcript variant 1
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $300.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "CD47 or NM_001777"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol CD47
Synonyms IAP; MER6; OA3
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC218813 representing NM_001777
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTGGCCCCTGGTAGCGGCGCTGTTGCTGGGCTCGGCGTGCTGCGGATCAGCTCAGCTACTATTTAATA
AAACAAAATCTGTAGAATTCACGTTTTGTAATGACACTGTCGTCATTCCATGCTTTGTTACTAATATGGA
GGCACAAAACACTACTGAAGTATACGTAAAGTGGAAATTTAAAGGAAGAGATATTTACACCTTTGATGGA
GCTCTAAACAAGTCCACTGTCCCCACTGACTTTAGTAGTGCAAAAATTGAAGTCTCACAATTACTAAAAG
GAGATGCCTCTTTGAAGATGGATAAGAGTGATGCTGTCTCACACACAGGAAACTACACTTGTGAAGTAAC
AGAATTAACCAGAGAAGGTGAAACGATCATCGAGCTAAAATATCGTGTTGTTTCATGGTTTTCTCCAAAT
GAAAATATTCTTATTGTTATTTTCCCAATTTTTGCTATACTCCTGTTCTGGGGACAGTTTGGTATTAAAA
CACTTAAATATAGATCCGGTGGTATGGATGAGAAAACAATTGCTTTACTTGTTGCTGGACTAGTGATCAC
TGTCATTGTCATTGTTGGAGCCATTCTTTTCGTCCCAGGTGAATATTCATTAAAGAATGCTACTGGCCTT
GGTTTAATTGTGACTTCTACAGGGATATTAATATTACTTCACTACTATGTGTTTAGTACAGCGATTGGAT
TAACCTCCTTCGTCATTGCCATATTGGTTATTCAGGTGATAGCCTATATCCTCGCTGTGGTTGGACTGAG
TCTCTGTATTGCGGCGTGTATACCAATGCATGGCCCTCTTCTGATTTCAGGTTTGAGTATCTTAGCTCTA
GCACAATTACTTGGACTAGTTTATATGAAATTTGTGGCTTCCAATCAGAAGACTATACAACCTCCTAGGA
AAGCTGTAGAGGAACCCCTTAATGCATTCAAAGAATCAAAAGGAATGATGAATGATGAA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC218813 representing NM_001777
Red=Cloning site Green=Tags(s)

MWPLVAALLLGSACCGSAQLLFNKTKSVEFTFCNDTVVIPCFVTNMEAQNTTEVYVKWKFKGRDIYTFDG
ALNKSTVPTDFSSAKIEVSQLLKGDASLKMDKSDAVSHTGNYTCEVTELTREGETIIELKYRVVSWFSPN
ENILIVIFPIFAILLFWGQFGIKTLKYRSGGMDEKTIALLVAGLVITVIVIVGAILFVPGEYSLKNATGL
GLIVTSTGILILLHYYVFSTAIGLTSFVIAILVIQVIAYILAVVGLSLCIAACIPMHGPLLISGLSILAL
AQLLGLVYMKFVASNQKTIQPPRKAVEEPLNAFKESKGMMNDE

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001777
ORF Size 969 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001777.4
RefSeq Size 5346 bp
RefSeq ORF 972 bp
Locus ID 961
UniProt ID Q08722
Cytogenetics 3q13.12
Domains CD47
Protein Families Druggable Genome, Transmembrane
Protein Pathways ECM-receptor interaction
MW 35.21 kDa
Summary This gene encodes a membrane protein, which is involved in the increase in intracellular calcium concentration that occurs upon cell adhesion to extracellular matrix. The encoded protein is also a receptor for the C-terminal cell binding domain of thrombospondin, and it may play a role in membrane transport and signal transduction. This gene has broad tissue distribution, and is reduced in expression on Rh erythrocytes. Alternatively spliced transcript variants have been found for this gene. provided by RefSeq, Jul 2010
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"NM_001777" in other vectors (6)
SKU Description Size Price
RC218813L1 Lenti ORF clone of Human CD47 molecule (CD47), transcript variant 1, Myc-DDK-tagged 10 ug
$600.00
RC218813L2 Lenti ORF clone of Human CD47 molecule (CD47), transcript variant 1, mGFP tagged 10 ug
$600.00
RC218813L3 Lenti ORF clone of Human CD47 molecule (CD47), transcript variant 1, Myc-DDK-tagged 10 ug
$600.00
RC218813L4 Lenti ORF clone of Human CD47 molecule (CD47), transcript variant 1, mGFP tagged 10 ug
$600.00
RG218813 CD47 (tGFP-tagged) - Human CD47 molecule (CD47), transcript variant 1 10 ug
$489.00 $500.00
SC119049 CD47 (untagged)-Human CD47 molecule (CD47), transcript variant 1 10 ug
$450.00

Plasmid Map for RC218813

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC218813
Circular map for RC218813

file-alt Citations

THE CIS ASSOCIATION OF CD47 WITH INTEGRIN Mac-1 REGULATES MACROPHAGE RESPONSES BY STABILIZING THE EXTENDED INTEGRIN CONFORMATION
Podolnikova, Key, Wang et al
J Biol Chem (2023) 299 (4), 103024 DOI: 10.1016/j.jbc.2023.103024
Cited by 16
AI Summary: OriGene’s CD47 plasmid (catalog # RC218813) was used to restore CD47 expression in knockout cells, enabling the authors to investigate the functional role of CD47 in macrophage responses.
Snippet: CD47-KO cells were transiently transfected with a plasmid encoding CD47 (OriGene, catalog # RC218813), and expression of CD47 was verified using mAb B6H12. The HEK293-based cell variants were maintained in DMEM/F12 medium (10-092-CM, Corning, Manassas, VA)
Large-scale generation of functional mRNA-encapsulating exosomes via cellular nanoporation
Yang, Shi, Xie et al
Nat Biomed Eng (2019) 4 (1), 69-83 DOI: 10.1038/s41551-019-0485-1
Cited by 741
AI Summary: In this study, OriGene's PTEN, CD47, CD63-GFP, and miR-128 plasmids were used to transfect source cells, enabling the production of exosomes loaded with specific mRNAs and peptides for therapeutic delivery via the cellular nanoporation method.
Snippet: …PTEN, CD47 CD63-GFP and miR-128 plasmids were purchased from Origene. Primers designed to encode CDX (FKESWREARGTRIERG)41, CREKA42 and Flag tag were used to introduce the ligands into the N terminus of CD47
Showing 1-2 of 2 papers.
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