NLRP8 (NM_176811) Human Tagged ORF Clone

SKU RC218775

NLRP8 (Myc-DDK-tagged)-Human NLR family, pyrin domain containing 8 (NLRP8)

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: NLRP8
• Synonyms: CLR19.2; NALP8; NOD16; PAN4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC218775 representing NM_176811; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGAGTGACGTGAATCCACCCTCTGACACCCCCATTCCCTTTTCATCCTCCTCCACTCACAGTTCTCATA; TTCCGCCCTGGACATTCTCTTGCTACCCCGGCTCCCCATGTGAAAATGGGGTCATGCTGTACATGAGAAA; CGTGAGCCATGAGGAGCTACAACGGTTCAAGCAGCTCTTACTGACTGAGCTCAGTACTGGCACCATGCCC; ATCACCTGGGACCAGGTCGAGACAGCCAGCTGGGCAGAGGTGGTTCATCTCTTGATAGAGCGTTTCCCTG; GACGACGCGCTTGGGATGTGACTTCGAACATCTTTGCCATTATGAACTGTGATAAAATGTGTGTTGTAGT; CCGCAGAGAGATAAATGCCATTCTGCCTACCTTGGAACCAGAGGACTTGAATGTGGGAGAAACACAGGTG; AATCTGGAGGAAGGAGAATCTGGTAAAATACGGCGGTATAAATCGAATGTGATGGAAAAGTTTTTCCCCA; TATGGGACATTACGACTTGGCCTGGAAACCAGAGGGACTTCTTCTACCAAGGTGTACACAGGCACGAGGA; GTACTTACCATGTCTGCTTCTGCCCAAAAGACCCCAGGGTAGACAGCCCAAGACCGTGGCCATACAGGGA; GCTCCTGGGATCGGAAAAACAATCCTGGCCAAAAAGGTGATGTTTGAGTGGGCCAGAAACAAGTTCTACG; CCCACAAGCGCTGGTGTGCTTTCTACTTCCATTGCCAAGAGGTGAACCAGACGACAGACCAGAGCTTCTC; CGAGCTGATTGAGCAAAAGTGGCCTGGATCTCAGGACCTCGTGTCAAAGATTATGTCCAAACCCGACCAA; CTTCTGCTGCTCTTGGATGGCTTTGAGGAGCTCACATCTACCCTCATTGACAGACTGGAGGACCTGAGTG; AAGACTGGAGGCAGAAATTGCCTGGGTCTGTCCTACTGAGCAGTTTGCTGAGCAAAACGATGCTTCCAGA; GGCCACGCTACTGATCATGATAAGATTTACCTCTTGGCAGACATGCAAGCCCTTGCTGAAATGTCCCTCT; CTCGTAACCCTTCCGGGGTTTAATACGATGGAAAAAATCAAGTATTTCCAGATGTATTTTGGACACACAG; AGGAGGGAGACCAAGTCTTGAGTTTCGCCATGGAAAACACCATTCTCTTCTCCATGTGCCGGGTCCCTGT; GGTTTGCTGGATGGTCTGCTCTGGTCTGAAACAGCAAATGGAGAGAGGAAACAATCTCACACAGTCATGT; CCAAATGCCACCTCTGTGTTCGTCCGGTATATTTCTAGCTTGTTTCCCACCAGAGCTGAGAACTTTTCCA; GAAAGATCCACCAAGCACAACTGGAAGGTCTGTGTCACTTGGCCGCAGACAGCATGTGGCACAGGAAATG; GGTGTTAGGTAAAGAAGATCTTGAGGAAGCCAAGCTGGATCAGACGGGAGTCACCGCCTTCCTTGGCATG; AGTATTCTTCGGAGAATTGCAGGTGAGGAAGACCACTATGTCTTTACCCTCGTGACTTTTCAGGAATTTT; TTGCGGCCTTGTTTTATGTTCTCTGTTTCCCACAAAGACTCAAAAATTTTCATGTGTTGAGCCACGTGAA; TATCCAGCGCCTGATAGCGAGTCCCAGAGGAAGCAAAAGCTATCTCTCTCACATGGGACTTTTCTTATTC; GGTTTTCTGAACGAGGCCTGCGCTTCGGCCGTGGAACAGTCATTCCAATGCAAGGTGTCTTTCGGTAATA; AGAGGAAACTGCTGAAAGTCATACCTCTGTTGCATAAATGTGACCCACCTTCTCCGGGCAGTGGGGTCCC; GCAGTTATTCTACTGTCTGCATGAAATCCGGGAGGAAGCCTTTGTAAGCCAAGCCCTAAATGATTATCAT; AAAGTTGTCTTGAGAATTGGCAACAACAAAGAAGTTCAAGTGTCTGCTTTTTGCCTGAAGCGGTGTCAAT; ATTTGCATGAGGTGGAACTGACCGTCACCCTGAACTTCATGAACGTGTGGAAGCTCAGCTCCAGCTCCCA; TCCTGGCTCTGAAGCGCCAGAGAGCAATGGGCTGCATCGTTGGTGGCAAGACTTATGCTCTGTGTTTGCA; ACGAATGATAAGCTGGAAGTCCTGACTATGACCAACAGTGTTTTGGGGCCTCCTTTTTTGAAGGCTCTCG; CGGCCGCACTGAGGCACCCTCAGTGCAAACTGCAAAAGCTACTCCTAAGGCGTGTGAATAGCACCATGTT; GAACCAGGACTTAATCGGTGTTTTGACGGGGAACCAGCATCTGAGATACTTGGAAATACAACATGTGGAA; GTGGAGTCCAAAGCTGTGAAGCTTCTATGCAGGGTGCTGAGATCCCCCCGGTGCCGTCTGCAGTGTCTCA; GGTTGGAAGACTGCTTGGCCACCCCTAGAATTTGGACTGATCTTGGCAATAATCTTCAAGGTAACGGGCA; TCTAAAGACTCTCATACTAAGAAAAAACTCCCTGGAGAACTGTGGGGCGTATTACCTGTCTGTGGCCCAG; CTGGAGAGGCTGTCGATAGAGAACTGCAACCTTACACAGCTTACTTGTGAAAGCCTTGCCTCCTGTCTCA; GGCAGAGTAAGATGCTGACCCACCTGAGCTTGGCAGAAAACGCCTTGAAAGATGAAGGGGCCAAGCATAT; TTGGAATGCCCTGCCACACCTGAGATGTCCTCTGCAGAGGCTGGTACTGAGAAAGTGTGACTTGACCTTT; AATTGCTGTCAGGATATGATCTCTGCGCTCTGTAAAAATAAAACCCTGAAAAGTCTTGACCTAAGTTTTA; ATAGCCTGAAGGATGATGGGGTGATCCTGCTGTGTGAGGCCCTGAAGAACCCTGACTGTACATTACAGAT; CCTGGAGCTGGAAAACTGCCTGTTCACCTCCATCTGCTGCCAGGCCATGGCTTCCATGCTCCGCAAAAAC; CAACATCTGAGACATCTGGACTTGAGCAAGAATGCGATTGGAGTCTATGGTATTCTGACCTTGTGCGAGG; CCTTCTCAAGCCAAAAGAAGAGAGAAGAGGTCATTTTCTGTATTCCTGCCTGGACTCGAATAACTAGCTT; CTCCCCAACTCCTCACCCACCCGACTTCACGGGAAAAAGTGACTGCCTATCCCAGATTAATCCT; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC218775 representing NM_176811; Red=Cloning site Green=Tags(s); ; MSDVNPPSDTPIPFSSSSTHSSHIPPWTFSCYPGSPCENGVMLYMRNVSHEELQRFKQLLLTELSTGTMP; ITWDQVETASWAEVVHLLIERFPGRRAWDVTSNIFAIMNCDKMCVVVRREINAILPTLEPEDLNVGETQV; NLEEGESGKIRRYKSNVMEKFFPIWDITTWPGNQRDFFYQGVHRHEEYLPCLLLPKRPQGRQPKTVAIQG; APGIGKTILAKKVMFEWARNKFYAHKRWCAFYFHCQEVNQTTDQSFSELIEQKWPGSQDLVSKIMSKPDQ; LLLLLDGFEELTSTLIDRLEDLSEDWRQKLPGSVLLSSLLSKTMLPEATLLIMIRFTSWQTCKPLLKCPS; LVTLPGFNTMEKIKYFQMYFGHTEEGDQVLSFAMENTILFSMCRVPVVCWMVCSGLKQQMERGNNLTQSC; PNATSVFVRYISSLFPTRAENFSRKIHQAQLEGLCHLAADSMWHRKWVLGKEDLEEAKLDQTGVTAFLGM; SILRRIAGEEDHYVFTLVTFQEFFAALFYVLCFPQRLKNFHVLSHVNIQRLIASPRGSKSYLSHMGLFLF; GFLNEACASAVEQSFQCKVSFGNKRKLLKVIPLLHKCDPPSPGSGVPQLFYCLHEIREEAFVSQALNDYH; KVVLRIGNNKEVQVSAFCLKRCQYLHEVELTVTLNFMNVWKLSSSSHPGSEAPESNGLHRWWQDLCSVFA; TNDKLEVLTMTNSVLGPPFLKALAAALRHPQCKLQKLLLRRVNSTMLNQDLIGVLTGNQHLRYLEIQHVE; VESKAVKLLCRVLRSPRCRLQCLRLEDCLATPRIWTDLGNNLQGNGHLKTLILRKNSLENCGAYYLSVAQ; LERLSIENCNLTQLTCESLASCLRQSKMLTHLSLAENALKDEGAKHIWNALPHLRCPLQRLVLRKCDLTF; NCCQDMISALCKNKTLKSLDLSFNSLKDDGVILLCEALKNPDCTLQILELENCLFTSICCQAMASMLRKN; QHLRHLDLSKNAIGVYGILTLCEAFSSQKKREEVIFCIPAWTRITSFSPTPHPPDFTGKSDCLSQINP; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Chromatograms: Chromatograms ; Sequencher program is needed, download here
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_176811
• ORF Size: 3144 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_176811.2, NP_789781.2
• RefSeq Size: 3934 bp
• RefSeq ORF: 3147 bp
• Locus ID: 126205
• UniProt ID: Q86W28
• Cytogenetics: 19q13.43
• MW: 119.2 kDa
• Summary: This gene encodes a member of the nucleotide-binding oligomerization domain/ leucine rich repeat/ pyrin domain containing (NLRP) subfamily, which belongs to the Nod-like receptor family of proteins. NLRP genes play roles in the mammalian innate immune system through inflammasome formation and activation of caspases. In addition, NLRP genes have been found to function during mammalian reproduction. Consistent with a function during human preimplantation development, this gene is expressed at high levels in oocytes with decreased levels in embryos. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2016]