FSH Receptor (FSHR) (NM_000145) Human Tagged ORF Clone

SKU
RC217736
FSHR (Myc-DDK-tagged)-Human follicle stimulating hormone receptor (FSHR), transcript variant 1
  • TrueORF®
    TrueORF®

    Expression-ready ORF plasmid with C-terminal tag(s)

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$700.00
2 Weeks*
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol FSH Receptor
Synonyms FSHR1; FSHRO; LGR1; ODG1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC217736 representing NM_000145
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCCTGCTCCTGGTCTCTTTGCTGGCATTCCTGAGCTTGGGCTCAGGATGTCATCATCGGATCTGTC
ACTGCTCTAACAGGGTTTTTCTCTGCCAAGAGAGCAAGGTGACAGAGATTCCTTCTGACCTCCCGAGGAA
TGCCATTGAACTGAGGTTTGTCCTCACCAAGCTTCGAGTCATCCAAAAAGGTGCATTTTCAGGATTTGGG
GACCTGGAGAAAATAGAGATCTCTCAGAATGATGTCTTGGAGGTGATAGAGGCAGATGTGTTCTCCAACC
TTCCCAAATTACATGAAATTAGAATTGAAAAGGCCAACAACCTGCTCTACATCAACCCTGAGGCCTTCCA
GAACCTTCCCAACCTTCAATATCTGTTAATATCCAACACAGGTATTAAGCACCTTCCAGATGTTCACAAG
ATTCATTCTCTCCAAAAAGTTTTACTTGACATTCAAGATAACATAAACATCCACACAATTGAAAGAAATT
CTTTCGTGGGGCTGAGCTTTGAAAGTGTGATTCTATGGCTGAATAAGAATGGGATTCAAGAAATACACAA
CTGTGCATTCAATGGAACCCAACTAGATGAGCTGAATCTAAGCGATAATAATAATTTAGAAGAATTGCCT
AATGATGTTTTCCACGGAGCCTCTGGACCAGTCATTCTAGATATTTCAAGAACAAGGATCCATTCCCTGC
CTAGCTATGGCTTAGAAAATCTTAAGAAGCTGAGGGCCAGGTCGACTTACAACTTAAAAAAGCTGCCTAC
TCTGGAAAAGCTTGTCGCCCTCATGGAAGCCAGCCTCACCTATCCCAGCCATTGCTGTGCCTTTGCAAAC
TGGAGACGGCAAATCTCTGAGCTTCATCCAATTTGCAACAAATCTATTTTAAGGCAAGAAGTTGATTATA
TGACTCAGGCTAGGGGTCAGAGATCCTCTCTGGCAGAAGACAATGAGTCCAGCTACAGCAGAGGATTTGA
CATGACGTACACTGAGTTTGACTATGACTTATGCAATGAAGTGGTTGACGTGACCTGCTCCCCTAAGCCA
GATGCATTCAACCCATGTGAAGATATCATGGGGTACAACATCCTCAGAGTCCTGATATGGTTTATCAGCA
TCCTGGCCATCACTGGGAACATCATAGTGCTAGTGATCCTAACTACCAGCCAATATAAACTCACAGTCCC
CAGGTTCCTTATGTGCAACCTGGCCTTTGCTGATCTCTGCATTGGAATCTACCTGCTGCTCATTGCATCA
GTTGATATCCATACCAAGAGCCAATATCACAACTATGCCATTGACTGGCAAACTGGGGCAGGCTGTGATG
CTGCTGGCTTTTTCACTGTCTTTGCCAGTGAGCTGTCAGTCTACACTCTGACAGCTATCACCTTGGAAAG
ATGGCATACCATCACGCATGCCATGCAGCTGGACTGCAAGGTGCAGCTCCGCCATGCTGCCAGTGTCATG
GTGATGGGCTGGATTTTTGCTTTTGCAGCTGCCCTCTTTCCCATCTTTGGCATCAGCAGCTACATGAAGG
TGAGCATCTGCCTGCCCATGGATATTGACAGCCCTTTGTCACAGCTGTATGTCATGTCCCTCCTTGTGCT
CAATGTCCTGGCCTTTGTGGTCATCTGTGGCTGCTATATCCACATCTACCTCACAGTGCGGAACCCCAAC
ATCGTGTCCTCCTCTAGTGACACCAGGATCGCCAAGCGCATGGCCATGCTCATCTTCACTGACTTCCTCT
GCATGGCACCCATTTCTTTCTTTGCCATTTCTGCCTCCCTCAAGGTGCCCCTCATCACTGTGTCCAAAGC
AAAGATTCTGCTGGTTCTGTTTCACCCCATCAACTCCTGTGCCAACCCCTTCCTCTATGCCATCTTTACC
AAAAACTTTCGCAGAGATTTCTTCATTCTGCTGAGCAAGTGTGGCTGCTATGAAATGCAAGCCCAAATTT
ATAGGACAGAAACTTCATCCACTGTCCACAACACCCATCCAAGGAATGGCCACTGCTCTTCAGCTCCCAG
AGTCACCAGTGGTTCCACTTACATACTTGTCCCTCTAAGTCATTTAGCCCAAAAC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC217736 representing NM_000145
Red=Cloning site Green=Tags(s)

MALLLVSLLAFLSLGSGCHHRICHCSNRVFLCQESKVTEIPSDLPRNAIELRFVLTKLRVIQKGAFSGFG
DLEKIEISQNDVLEVIEADVFSNLPKLHEIRIEKANNLLYINPEAFQNLPNLQYLLISNTGIKHLPDVHK
IHSLQKVLLDIQDNINIHTIERNSFVGLSFESVILWLNKNGIQEIHNCAFNGTQLDELNLSDNNNLEELP
NDVFHGASGPVILDISRTRIHSLPSYGLENLKKLRARSTYNLKKLPTLEKLVALMEASLTYPSHCCAFAN
WRRQISELHPICNKSILRQEVDYMTQARGQRSSLAEDNESSYSRGFDMTYTEFDYDLCNEVVDVTCSPKP
DAFNPCEDIMGYNILRVLIWFISILAITGNIIVLVILTTSQYKLTVPRFLMCNLAFADLCIGIYLLLIAS
VDIHTKSQYHNYAIDWQTGAGCDAAGFFTVFASELSVYTLTAITLERWHTITHAMQLDCKVQLRHAASVM
VMGWIFAFAAALFPIFGISSYMKVSICLPMDIDSPLSQLYVMSLLVLNVLAFVVICGCYIHIYLTVRNPN
IVSSSSDTRIAKRMAMLIFTDFLCMAPISFFAISASLKVPLITVSKAKILLVLFHPINSCANPFLYAIFT
KNFRRDFFILLSKCGCYEMQAQIYRTETSSTVHNTHPRNGHCSSAPRVTSGSTYILVPLSHLAQN

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene Plasmid Map
ACCN NM_000145
ORF Size 2085 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_000145.4
RefSeq Size 2382 bp
RefSeq ORF 2088 bp
Locus ID 2492
UniProt ID P23945
Cytogenetics 2p16.3
Protein Families Druggable Genome, ES Cell Differentiation/IPS, GPCR, Transmembrane
Protein Pathways Neuroactive ligand-receptor interaction
MW 78.24 kDa
Summary The protein encoded by this gene belongs to family 1 of G-protein coupled receptors. It is the receptor for follicle stimulating hormone and functions in gonad development. Mutations in this gene cause ovarian dysgenesis type 1, and also ovarian hyperstimulation syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2010]
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Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.