TRIM58 (NM_015431) Human Tagged ORF Clone

CAT#: RC217706

Reviews ()
Write a review

TRIM58 (Myc-DDK-tagged)-Human tripartite motif containing 58 (TRIM58)



  "NM_015431" in other vectors (6)

Reconstitution Protocol

Special Offer: 20% off the control vector (empty cloning vector without insert). Use code "Shuttle20".

USD 511.00


Availability*
In Stock

Size
    • 10 ug


Product images

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol TRIM58
Synonyms BIA2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC217706 representing NM_015431
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCTCGGCGCCGCCCGGGGAGCGGCTGCGCGAGGATGCGCGGTGCCCGGTGTGCCTGGATTTCCTGC
AGGAGCCGGTCAGCGTGGACTGCGGCCACAGCTTCTGCCTCAGGTGCATCTCCGAGTTCTGCGAGAAGTC
GGACGGCGCGCAGGGCGGCGTCTACGCCTGTCCGCAGTGCCGGGGCCCCTTCCGGCCCTCGGGCTTTCGC
CCCAACCGGCAGCTGGCGGGCCTGGTGGAGAGCGTGCGGCGGCTGGGGTTGGGCGCGGGGCCCGGGGCGC
GGCGATGCGCGCGGCACGGCGAGGACCTGAGCCGCTTCTGCGAGGAGGACGAGGCGGCGCTGTGCTGGGT
GTGCGACGCCGGCCCCGAGCACAGGACGCACCGCACGGCGCCGCTGCAGGAGGCCGCCGGCAGCTACCAG
GTAAAGCTCCAGATGGCTCTGGAACTTATGAGGAAAGAGTTGGAGGACGCCTTGACTCAGGAGGCCAACG
TGGGGAAAAAGACTGTCATTTGGAAGGAGAAAGTGGAAATGCAGAGGCAGCGCTTCAGATTGGAGTTTGA
GAAGCATCGTGGCTTTCTGGCCCAGGAGGAGCAACGGCAGCTGAGGCGGCTGGAGGCGGAGGAGCGAGCG
ACGCTGCAGAGACTGCGGGAGAGCAAGAGCCGGCTGGTCCAGCAGAGCAAGGCCCTGAAGGAGCTGGCGG
ATGAGCTGCAGGAGAGGTGCCAGCGCCCGGCCCTGGGTCTGCTGGAGGGTGTGAGAGGAGTCCTGAGCAG
AAGTAAGGCTGTCACAAGGCTGGAAGCAGAGAACATCCCCATGGAACTGAAGACAGCATGCTGCATCCCT
GGGAGGAGGGAGCTCTTAAGGAAGTTCCAAGTGGATGTAAAGCTGGATCCCGCCACGGCGCACCCGAGTC
TGCTCTTGACCGCCGACCTGCGCAGTGTGCAGGATGGAGAACCATGGAGGGATGTCCCCAACAACCCTGA
GCGATTTGACACATGGCCCTGCATCCTGGGTTTGCAGAGCTTCTCATCAGGGAGGCATTACTGGGAGGTT
CTGGTGGGAGAAGGAGCAGAGTGGGGTTTAGGGGTCTGTCAAGACACACTGCCAAGAAAGGGGGAAACCA
CGCCATCTCCTGAGAATGGGGTCTGGGCCCTGTGGCTGCTGAAAGGGAATGAGTACATGGTCCTTGCCTC
CCCATCAGTGCCTCTTCTCCAACTGGAAAGTCCTCGCTGCATTGGGATTTTCTTGGACTATGAAGCCGGT
GAAATTTCATTCTACAATGTCACAGATGGATCTTATATCTACACATTCAACCAACTCTTCTCTGGTCTTC
TTCGGCCTTACTTTTTCATCTGTGATGCAACTCCTCTTATCTTGCCACCCACAACAATAGCAGGGTCAGG
AAATTGGGCATCCAGGGATCATTTAGATCCTGCTTCTGATGTAAGAGATGATCATCTC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC217706 representing NM_015431
Red=Cloning site Green=Tags(s)

MASAPPGERLREDARCPVCLDFLQEPVSVDCGHSFCLRCISEFCEKSDGAQGGVYACPQCRGPFRPSGFR
PNRQLAGLVESVRRLGLGAGPGARRCARHGEDLSRFCEEDEAALCWVCDAGPEHRTHRTAPLQEAAGSYQ
VKLQMALELMRKELEDALTQEANVGKKTVIWKEKVEMQRQRFRLEFEKHRGFLAQEEQRQLRRLEAEERA
TLQRLRESKSRLVQQSKALKELADELQERCQRPALGLLEGVRGVLSRSKAVTRLEAENIPMELKTACCIP
GRRELLRKFQVDVKLDPATAHPSLLLTADLRSVQDGEPWRDVPNNPERFDTWPCILGLQSFSSGRHYWEV
LVGEGAEWGLGVCQDTLPRKGETTPSPENGVWALWLLKGNEYMVLASPSVPLLQLESPRCIGIFLDYEAG
EISFYNVTDGSYIYTFNQLFSGLLRPYFFICDATPLILPPTTIAGSGNWASRDHLDPASDVRDDHL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_015431
ORF Size 1458 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_015431.2, NP_056246.2
RefSeq Size 2594 bp
RefSeq ORF 1461 bp
Locus ID 25893
UniProt ID Q8NG06
Cytogenetics 1q44
Domains zf-B_box, RING, SPRY, PRY
Protein Families Druggable Genome
MW 54.6 kDa
Gene Summary E3 ubiquitin ligase induced during late erythropoiesis. Directly binds and ubiquitinates the intermediate chain of the microtubule motor dynein (DYNC1LI1/DYNC1LI2), stimulating the degradation of the dynein holoprotein complex. May participate in the erythroblast enucleation process through regulation of nuclear polarization.[UniProtKB/Swiss-Prot Function]

Other Versions

Other products for "TRIM58"
Frequently bought together (5)
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody
    • 30 ul

USD 165.00


pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

USD 627.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

USD 149.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

USD 55.00


Rabbit Polyclonal Anti-TRIM58 Antibody
    • 100 ug

USD 484.00

Customer Reviews 
Loading...
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.