ROR alpha (RORA) (NM_134260) Human Tagged ORF Clone

SKU RC216664

RORA (Myc-DDK-tagged)-Human RAR-related orphan receptor A (RORA), transcript variant 2

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: ROR alpha
• Synonyms: IDDECA; NR1F1; ROR1; ROR2; ROR3; RZR-ALPHA; RZRA
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC216664 representing NM_134260; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGAATGAGGGGGCCCCAGGAGACAGTGACTTAGAGACTGAGGCAAGAGTGCCGTGGTCAATCATGGGTC; ATTGTCTTCGAACTGGACAGGCCAGAATGTCTGCCACACCCACACCTGCAGGTGAAGGAGCCAGAAGGGA; TGAACTTTTTGGGATTCTCCAAATACTCCATCAGTGTATCCTGTCTTCAGGTGATGCTTTTGTTCTTACT; GGCGTCTGTTGTTCCTGGAGGCAGAATGGCAAGCCACCATATTCACAAAAGGAAGATAAGGAAGTACAAA; CTGGATACATGAATGCTCAAATTGAAATTATTCCATGCAAGATCTGTGGAGACAAATCATCAGGAATCCA; TTATGGTGTCATTACATGTGAAGGCTGCAAGGGCTTTTTCAGGAGAAGTCAGCAAAGCAATGCCACCTAC; TCCTGTCCTCGTCAGAAGAACTGTTTGATTGATCGAACCAGTAGAAACCGCTGCCAACACTGTCGATTAC; AGAAATGCCTTGCCGTAGGGATGTCTCGAGATGCTGTAAAATTTGGCCGAATGTCAAAAAAGCAGAGAGA; CAGCTTGTATGCAGAAGTACAGAAACACCGGATGCAGCAGCAGCAGCGCGACCACCAGCAGCAGCCTGGA; GAGGCTGAGCCGCTGACGCCCACCTACAACATCTCGGCCAACGGGCTGACGGAACTTCACGACGACCTCA; GTAACTACATTGACGGTCACACCCCTGAGGGGATTAAGGCAGACTCCGCCGTCAGCAGCTTCTACCTGGA; CATACAGCCTTCCCCAGACCAGTCAGGTCTTGATATCAATGGAATCAAACCAGAACCAATATGTGACTAC; ACACCAGCATCAGGCTTCTTTCCCTACTGTTCGTTCACCAACGGCGAGACTTCCCCAACTGTGTCCATGG; CAGAATTAGAACACCTTGCACAGAATATATCTAAATCGCATCTGGAAACCTGCCAATACTTGAGAGAAGA; GCTCCAGCAGATAACGTGGCAGACCTTTTTACAGGAAGAAATTGAGAACTATCAAAACAAGCAGCGGGAG; GTGATGTGGCAATTGTGTGCCATCAAAATTACAGAAGCTATACAGTATGTGGTGGAGTTTGCCAAACGCA; TTGATGGATTTATGGAACTGTGTCAAAATGATCAAATTGTGCTTCTAAAAGCAGGTTCTCTAGAGGTGGT; GTTTATCAGAATGTGCCGTGCCTTTGACTCTCAGAACAACACCGTGTACTTTGATGGGAAGTATGCCAGC; CCCGACGTCTTCAAATCCTTAGGTTGTGAAGACTTTATTAGCTTTGTGTTTGAATTTGGAAAGAGTTTAT; GTTCTATGCACCTGACTGAAGATGAAATTGCATTATTTTCTGCATTTGTACTGATGTCAGCAGATCGCTC; ATGGCTGCAAGAAAAGGTAAAAATTGAAAAACTGCAACAGAAAATTCAGCTAGCTCTTCAACACGTCCTA; CAGAAGAATCACCGAGAAGATGGAATACTAACAAAGTTAATATGCGAGGTGTCTACATTAAGAGCCTTAT; GTGGACGACATACAGAAAAGCTAATGGCATTTAAAGCAATATACCCAGACATTGTGCGACTTCATTTTCC; TCCATTATACAAGGAGTTGTTCACTTCAGAATTTGAGCCAGCAATGCAAATTGATGGG; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC216664 representing NM_134260; Red=Cloning site Green=Tags(s); ; MNEGAPGDSDLETEARVPWSIMGHCLRTGQARMSATPTPAGEGARRDELFGILQILHQCILSSGDAFVLT; GVCCSWRQNGKPPYSQKEDKEVQTGYMNAQIEIIPCKICGDKSSGIHYGVITCEGCKGFFRRSQQSNATY; SCPRQKNCLIDRTSRNRCQHCRLQKCLAVGMSRDAVKFGRMSKKQRDSLYAEVQKHRMQQQQRDHQQQPG; EAEPLTPTYNISANGLTELHDDLSNYIDGHTPEGIKADSAVSSFYLDIQPSPDQSGLDINGIKPEPICDY; TPASGFFPYCSFTNGETSPTVSMAELEHLAQNISKSHLETCQYLREELQQITWQTFLQEEIENYQNKQRE; VMWQLCAIKITEAIQYVVEFAKRIDGFMELCQNDQIVLLKAGSLEVVFIRMCRAFDSQNNTVYFDGKYAS; PDVFKSLGCEDFISFVFEFGKSLCSMHLTEDEIALFSAFVLMSADRSWLQEKVKIEKLQQKIQLALQHVL; QKNHREDGILTKLICEVSTLRALCGRHTEKLMAFKAIYPDIVRLHFPPLYKELFTSEFEPAMQIDG; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Chromatograms: Chromatograms ; Sequencher program is needed, download here
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_134260
• ORF Size: 1668 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_134260.2
• RefSeq Size: 2020 bp
• RefSeq ORF: 1671 bp
• Locus ID: 6095
• UniProt ID: P35398
• Cytogenetics: 15q22.2
• Protein Families: Druggable Genome, Nuclear Hormone Receptor, Transcription Factors
• MW: 62.9 kDa
• Summary: The protein encoded by this gene is a member of the NR1 subfamily of nuclear hormone receptors. It can bind as a monomer or as a homodimer to hormone response elements upstream of several genes to enhance the expression of those genes. The encoded protein has been shown to interact with NM23-2, a nucleoside diphosphate kinase involved in organogenesis and differentiation, as well as with NM23-1, the product of a tumor metastasis suppressor candidate gene. Also, it has been shown to aid in the transcriptional regulation of some genes involved in circadian rhythm. Four transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Feb 2014]