TMEM41B (NM_015012) Human Tagged ORF Clone

TrueORF Gold

Protein expression verified by Western blot, fully sequenced and in stock

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SKU

RC216351

TMEM41B (Myc-DDK-tagged)-Human transmembrane protein 41B (TMEM41B), transcript variant 1

Datasheet SDS Plasmid Map

Reconstitution Protocol

NM_015012 in other vectors (4)

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$300.00

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Bulk Requests & Clone Modifications

Frequently Bought Together (5)

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CC100003	DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)	5 x 200 ul	$160.00
PRIMER	Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each	100 pmol	$59.00
PS100001	pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug	10 ug	$650.00
AR100028M	DDKTrap Anti-DDK 8G6 affinity resin used for the purification of DDK, DYKDDDDK, or FLAG® tagged proteins , 5 mL	5 ml	$720.00

Specifications

Specifications
Product Data
Type	Human Tagged ORF Clone
Target Symbol	TMEM41B
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence >RC216351 ORF sequence Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGAAAGGCAGAGTCGCCGAACGATCGCAGTTGGGCGCTCACCACACGACCCCCGTGGGGGACGGGG CAGCGGGGACGCGGGGTCTCGCGGCGCCTGGCAGCAGAGACCACCAGAAGGAAAAATCCTGGGTAGAAGC TGGATCAGCAAGAATGTCACTCCTTATATTGGTGTCCATTTTCTTATCTGCAGCTTTTGTTATGTTTTTG GTATATAAAAATTTTCCTCAGCTTAGTGAAGAAGAAAGAGTGAATATGAAGGTTCCCAGAGATATGGATG ATGCCAAGGCTCTAGGAAAAGTTTTATCCAAATACAAGGACACCTTTTATGTTCAAGTACTTGTAGCTTA TTTTGCTACATATATTTTCTTGCAAACATTTGCTATTCCAGGCTCTATATTTCTCAGTATACTCTCAGGG TTTCTTTATCCCTTTCCACTAGCCTTATTTCTTGTTTGTTTGTGTTCTGGACTTGGTGCCTCTTTCTGTT ATATGCTTTCCTATTTAGTTGGGAGACCAGTTGTATACAAATACCTAACAGAGAAAGCAGTAAAATGGTC ACAGCAGGTTGAACGTCATAGAGAACATCTCATTAACTACATTATATTTTTGAGAATAACACCATTTCTG CCTAATTGGTTTATTAATATCACATCTCCTGTGATAAACGTGCCATTGAAAGTTTTTTTTATTGGTACTT TTCTAGGTGTCGCACCTCCTTCTTTTGTAGCCATTAAGGCAGGAACAACACTGTATCAACTTACAACAGC AGGAGAAGCTGTTTCCTGGAACTCAATATTTATTCTGATGATCTTGGCTGTTCTTTCTATTCTGCCAGCC ATCTTCCAAAAAAAACTAAAGCAGAAATTTGAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence >RC216351 protein sequence Red=Cloning site Green=Tags(s) MAKGRVAERSQLGAHHTTPVGDGAAGTRGLAAPGSRDHQKEKSWVEAGSARMSLLILVSIFLSAAFVMFL VYKNFPQLSEEERVNMKVPRDMDDAKALGKVLSKYKDTFYVQVLVAYFATYIFLQTFAIPGSIFLSILSG FLYPFPLALFLVCLCSGLGASFCYMLSYLVGRPVVYKYLTEKAVKWSQQVERHREHLINYIIFLRITPFL PNWFINITSPVINVPLKVFFIGTFLGVAPPSFVAIKAGTTLYQLTTAGEAVSWNSIFILMILAVLSILPA IFQKKLKQKFE TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms	Chromatograms Sequencher program is needed, download here
Restriction Sites	SgfI-MluI Cloning Scheme for this gene Plasmid Map
ACCN	NM_015012
ORF Size	873 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping	Ambient
Reference Data
RefSeq	NM_015012.4
RefSeq Size	3973 bp
RefSeq ORF	876 bp
Locus ID	440026
UniProt ID	Q5BJD5
Cytogenetics	11p15.4
Protein Families	Transmembrane
MW	32.5 kDa
Summary	Required for normal motor neuron development (By similarity). Required for autophagosome formation (PubMed:30093494).[UniProtKB/Swiss-Prot Function]

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Documents

Product Manuals
TrueORFs: Open Reading Frame Clones - 10 ug in PrecisionShuttle Vector System TrueClones: Full length cDNA clones (Human/Mouse/Rat), expressing native proteins pLenti-ORF destination vector Transfection - TurboFectin 8.0 Transfection Reagent Plasmid Preparation - PowerPrep Plasmid Purification Kits
FAQs
TrueORF Clones/PrecisionShuttle Organelle Marker Transfection Reagent
SDS
SDS for RC216351

Resources

cDNA Clone Resources
Video Tutorial: Clone Selection Guide Product Video: What is TrueORF GOLD and why it is the most popular type of clone Video Tutorial: How to make Lentivirus particles Video Tutorial: Is the Lentivirus Safe Product Brochure: cDNA clones Protocol: Lentivirus packaging Product Sheet: AAV AAV Biosafety Lentivirus and AAV FAQ Virus-Mediated Gene Delivery

"NM_015012" in other vectors (4)

SKU	Description	Size	Price
RC216351L3	Lenti ORF clone of Human transmembrane protein 41B (TMEM41B), transcript variant 1, Myc-DDK-tagged	10 ug	$600.00
RC216351L4	Lenti ORF clone of Human transmembrane protein 41B (TMEM41B), transcript variant 1, mGFP tagged	10 ug	$600.00
RG216351	TMEM41B (tGFP-tagged) - Human transmembrane protein 41B (TMEM41B), transcript variant 1	10 ug	$489.00 $500.00 $500.00
SC304205	TMEM41B (untagged)-Human transmembrane protein 41B (TMEM41B), transcript variant 1	10 ug	$330.00

Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.