BRN3A (POU4F1) (NM_006237) Human Tagged ORF Clone

SKU
RC216284
POU4F1 (Myc-DDK-tagged)-Human POU class 4 homeobox 1 (POU4F1)
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Specifications
Product Data
Target Symbol BRN3A
Synonyms ATITHS; brn-3A; BRN3A; Oct-T1; RDC-1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC216284 representing NM_006237
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGATGTCCATGAACAGCAAGCAGCCTCACTTTGCCATGCATCCCACCCTCCCTGAGCACAAGTACCCGT
CGCTGCACTCCAGCTCCGAGGCCATCCGGCGGGCCTGCCTGCCCACGCCGCCGCTGCAGAGCAACCTCTT
CGCCAGCCTGGACGAGACGCTGCTGGCGCGGGCCGAGGCGCTGGCGGCCGTGGACATCGCCGTGTCCCAG
GGCAAGAGCCATCCTTTCAAGCCGGACGCCACGTACCACACGATGAACAGCGTGCCGTGCACGTCCACTT
CCACGGTGCCTCTGGCGCACCACCACCACCACCACCACCACCACCAGGCGCTCGAACCCGGCGATCTGCT
GGACCACATCTCCTCGCCGTCGCTCGCGCTCATGGCCGGCGCGGGCGGCGCGGGCGCGGCGGCCGGCGGC
GGCGGCGCCCACGACGGCCCGGGGGGCGGTGGCGGCCCGGGCGGCGGCGGCGGCCCGGGCGGCGGCCCCG
GGGGAGGCGGCGGTGGCGGCCCGGGGGGCGGCGGCGGCGGCCCGGGCGGCGGGCTCCTGGGCGGCTCCGC
GCACCCTCACCCGCATATGCACAGCCTGGGCCACCTGTCGCACCCCGCGGCGGCGGCCGCCATGAACATG
CCGTCCGGGCTGCCGCACCCCGGGCTGGTGGCGGCGGCGGCGCACCACGGCGCGGCAGCGGCAGCGGCGG
CGGCGGCGGCCGGGCAGGTGGCAGCGGCATCGGCGGCGGCGGCCGTGGTGGGCGCAGCGGGCCTGGCGTC
CATCTGCGACTCGGACACGGACCCGCGCGAGCTCGAGGCGTTCGCGGAGCGCTTCAAGCAGCGGCGCATC
AAGCTGGGCGTGACGCAGGCCGACGTGGGCTCGGCGCTGGCCAACCTCAAGATCCCGGGCGTGGGCTCAC
TCAGCCAGAGCACCATCTGCAGGTTCGAGTCGCTCACGCTCTCGCACAACAACATGATCGCGCTCAAGCC
CATCCTGCAGGCGTGGCTCGAGGAGGCCGAGGGCGCCCAGCGCGAGAAAATGAACAAGCCTGAGCTCTTC
AACGGCGGCGAGAAGAAGCGCAAGCGGACTTCCATCGCCGCGCCCGAGAAGCGCTCCCTCGAGGCCTACT
TCGCCGTGCAGCCCCGGCCCTCGTCCGAGAAGATCGCCGCCATCGCCGAGAAACTGGACCTCAAAAAGAA
CGTGGTGCGGGTGTGGTTTTGCAACCAGAGACAGAAGCAGAAGCGGATGAAATTCTCTGCCACTTAC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC216284 representing NM_006237
Red=Cloning site Green=Tags(s)

MMSMNSKQPHFAMHPTLPEHKYPSLHSSSEAIRRACLPTPPLQSNLFASLDETLLARAEALAAVDIAVSQ
GKSHPFKPDATYHTMNSVPCTSTSTVPLAHHHHHHHHHQALEPGDLLDHISSPSLALMAGAGGAGAAAGG
GGAHDGPGGGGGPGGGGGPGGGPGGGGGGGPGGGGGGPGGGLLGGSAHPHPHMHSLGHLSHPAAAAAMNM
PSGLPHPGLVAAAAHHGAAAAAAAAAAGQVAAASAAAAVVGAAGLASICDSDTDPRELEAFAERFKQRRI
KLGVTQADVGSALANLKIPGVGSLSQSTICRFESLTLSHNNMIALKPILQAWLEEAEGAQREKMNKPELF
NGGEKKRKRTSIAAPEKRSLEAYFAVQPRPSSEKIAAIAEKLDLKKNVVRVWFCNQRQKQKRMKFSATY

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene Plasmid Map
ACCN NM_006237
ORF Size 1257 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_006237.4
RefSeq Size 3824 bp
RefSeq ORF 1260 bp
Locus ID 5457
UniProt ID Q01851
Cytogenetics 13q31.1
Protein Families Transcription Factors
MW 42.5 kDa
Summary This gene encodes a member of the POU-IV class of neural transcription factors. This protein is expressed in a subset of retinal ganglion cells and may be involved in the developing sensory nervous system. This protein may also promote the growth of cervical tumors. A translocation of this gene is associated with some adult acute myeloid leukemias. [provided by RefSeq, Mar 2012]
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SKU Description Size Price
RC216284L1 Lenti ORF clone of Human POU class 4 homeobox 1 (POU4F1), Myc-DDK-tagged 10 ug
$757.00
RC216284L2 Lenti ORF clone of Human POU class 4 homeobox 1 (POU4F1), mGFP tagged 10 ug
$757.00
RC216284L3 Lenti ORF clone of Human POU class 4 homeobox 1 (POU4F1), Myc-DDK-tagged 10 ug
$757.00
RC216284L4 Lenti ORF clone of Human POU class 4 homeobox 1 (POU4F1), mGFP tagged 10 ug
$757.00
RG216284 POU4F1 (tGFP-tagged) - Human POU class 4 homeobox 1 (POU4F1) 10 ug
$657.00
SC303757 POU4F1 (untagged)-Human POU class 4 homeobox 1 (POU4F1) 10 ug
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Citations

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