SPIRE2 (NM_032451) Human Tagged ORF Clone

SKU RC215650

SPIRE2 (Myc-DDK-tagged)-Human spire homolog 2 (Drosophila) (SPIRE2)

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: SPIRE2
• Synonyms: Spir-2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC215650 representing NM_032451; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGCCCGGGCGGGCAGCTGCGGCGGCGCCGCGGCGGGCGCAGGGCGGCCGGAGCCCTGGGAGCTGTCCC; TGGAGGAGGTGCTGAAGGCCTACGAGCAGCCGCTCAACGAGGAGCAGGCGTGGGCCGTGTGCTTCCAGGG; CTGCCGCGGGCTGCGGGGCTCGCCGGGCCGGCGCCTGCGGGATACCGGGGACCTCCTGCTGCGCGGGGAC; GGCTCGGTCGGGGCGCGGGAGCCCGAGGCCGCGGAACCTGCAACCATGGTCGTGCCACTAGCCAGCTCGG; AAGCCCAGACCGTGCAGTCCCTCGGCTTCGCCATCTACCGCGCGCTGGACTGGGGGCTGGACGAGAGCGA; GGAGCGCGAACTCAGCCCTCAGCTGGAGCGGCTCATCGACCTCATGGCCAACAACGACAGCGAGGACAGC; GGCTGCGGTGCCGCCGATGAGGGCTACGGGGGTCCCGAGGAGGAGGAGGAGGCCGAGGGCGTCCCCCGCA; GCGTGCGCACCTTTGCCCAGGCCATGCGGCTGTGCGCGGCGCGGCTGACCGACCCCCGGGGCGCACAGGC; GCATTACCAGGCCGTGTGCCGCGCGCTCTTCGTGGAGACGCTGGAGCTGCGGGCCTTCCTGGCCAGGGTC; CGGGAGGCCAAGGAGATGCTGCAGAAGCTTCGGGAGGACGAGCCGCATCTGGAGACGCCTCGGGCAGAGC; TGGACAGCCTGGGTCACACAGACTGGGCCCGACTGTGGGTTCAGCTCATGCGGGAGCTCCGCCGCGGAGT; GAAGCTGAAGAAGGTGCAAGAGCAGGAGTTCAACCCCCTCCCCACCGAGTTCCAGCTCACGCCCTTCGAG; ATGCTGATGCAGGACATCCGGGCCCGGAACTACAAGCTGCGCAAGGTCATGGTGGATGGGGACATCCCGC; CCCGGGTGAAGAAGGACGCTCACGAGCTCATCCTGGACTTTATCCGCTCACGGCCTCCACTGAAGCAGGT; CTCTGAGAGGCGGCTGCGCCCGTTGCCACCAAAGCAAAGGTCCCTGCATGAGAAGATCCTGGAGGAGATC; AAGCAGGAGCGGAGGCTGCGCCCGGTGCGGGGCGAGGGCTGGGCTGCCCGCGGGTTTGGCTCTCTGCCCT; GCATCCTCAACGCCTGCTCCGGAGATGCCAAGTCCACCTCCTGCATCAACCTGTCAGTCACAGATGCTGG; GGGCAGCGCCCAGCGCCCGCGGCCCCGCGTGCTGCTCAAGGCGCCTACCTTGGCTGAAATGGAAGAGATG; AATACATCTGAGGAAGAAGAGTCTCCGTGTGGGGAGGTGACGCTGAAACGGGACCGCTCCTTCTCAGAGC; ATGACCTGGCCCAGCTCCGAAGTGAGGTGGCCTCTGGCCTGCAGTCGGCCACCCACCCCCCAGGAGGGAC; GGAGCCACCACGGCCCCGAGCTGGCAGTGCGCATGTGTGGAGGCCCGGCTCCCGAGACCAGGGTACCTGT; CCCGCGAGTGTCTCTGACCCCAGCCACCCCCTACTCAGCAACCGGGGCTCCTCGGGGGACAGACCCGAGG; CCTCCATGACCCCCGATGCCAAACACCTGTGGCTGGAGTTCAGCCACCCCGTGGAGAGCCTGGCGCTGAC; TGTGGAAGAGGTGATGGACGTGCGCCGTGTGCTGGTGAAGGCCGAGATGGAAAAGTTTTTGCAGAACAAG; GAGCTCTTCAGCAGTCTGAAGAAGGGGAAGATTTGCTGCTGCTGCCGGGCCAAGTTCCCGCTGTTCTCGT; GGCCGCCCAGCTGTCTCTTCTGCAAGAGAGCCGTCTGCACTTCCTGTAGCATAAAGATGAAGATGCCTTC; TAAGAAATTTGGACACATCCCTGTCTACACACTGGGCTTTGAGAGTCCTCAGAGGGTATCAGCTGCCAAA; ACCGCGCCAATCCAGAGAAGAGACATCTTTCAGTCTCTGCAAGGGCCACAGTGGCAGAGCGTGGAGGAGG; CGTTCCCCCACATCTACTCCCACGGCTGTGTCCTGAAGGATGTCTGCAGTGAGTGCACCAGCTTTGTGGC; AGACGTGGTGCGTTCCAGCCGCAAGAGCGTGGACGTCCTCAACACTACGCCACGACGCAGTCGCCAGACC; CAATCCCTCTACATCCCTAACACCAGGACTCTTGACTTCAAG; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC215650 representing NM_032451; Red=Cloning site Green=Tags(s); ; MARAGSCGGAAAGAGRPEPWELSLEEVLKAYEQPLNEEQAWAVCFQGCRGLRGSPGRRLRDTGDLLLRGD; GSVGAREPEAAEPATMVVPLASSEAQTVQSLGFAIYRALDWGLDESEERELSPQLERLIDLMANNDSEDS; GCGAADEGYGGPEEEEEAEGVPRSVRTFAQAMRLCAARLTDPRGAQAHYQAVCRALFVETLELRAFLARV; REAKEMLQKLREDEPHLETPRAELDSLGHTDWARLWVQLMRELRRGVKLKKVQEQEFNPLPTEFQLTPFE; MLMQDIRARNYKLRKVMVDGDIPPRVKKDAHELILDFIRSRPPLKQVSERRLRPLPPKQRSLHEKILEEI; KQERRLRPVRGEGWAARGFGSLPCILNACSGDAKSTSCINLSVTDAGGSAQRPRPRVLLKAPTLAEMEEM; NTSEEEESPCGEVTLKRDRSFSEHDLAQLRSEVASGLQSATHPPGGTEPPRPRAGSAHVWRPGSRDQGTC; PASVSDPSHPLLSNRGSSGDRPEASMTPDAKHLWLEFSHPVESLALTVEEVMDVRRVLVKAEMEKFLQNK; ELFSSLKKGKICCCCRAKFPLFSWPPSCLFCKRAVCTSCSIKMKMPSKKFGHIPVYTLGFESPQRVSAAK; TAPIQRRDIFQSLQGPQWQSVEEAFPHIYSHGCVLKDVCSECTSFVADVVRSSRKSVDVLNTTPRRSRQT; QSLYIPNTRTLDFK; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Chromatograms: Chromatograms ; Sequencher program is needed, download here
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_032451
• ORF Size: 2142 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_032451.2
• RefSeq Size: 3249 bp
• RefSeq ORF: 2145 bp
• Locus ID: 84501
• UniProt ID: Q8WWL2
• Cytogenetics: 16q24.3
• Protein Pathways: Dorso-ventral axis formation
• MW: 79.5 kDa
• Summary: Acts as an actin nucleation factor, remains associated with the slow-growing pointed end of the new filament (PubMed:21620703). Involved in intracellular vesicle transport along actin fibers, providing a novel link between actin cytoskeleton dynamics and intracellular transport (By similarity). Required for asymmetric spindle positioning and asymmetric cell division during meiosis (PubMed:21620703). Required for normal formation of the cleavage furrow and for polar body extrusion during female germ cell meiosis (PubMed:21620703). Also acts in the nucleus: together with SPIRE1 and SPIRE2, promotes assembly of nuclear actin filaments in response to DNA damage in order to facilitate movement of chromatin and repair factors after DNA damage (PubMed:26287480).[UniProtKB/Swiss-Prot Function]