B7H3 (CD276) (NM_025240) Human Tagged ORF Clone
$300.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | B7H3 |
| Synonyms | 4Ig-B7-H3; B7-H3; B7H3; B7RP-2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_025240 |
| ORF Size | 948 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_025240.2, NP_079516.1 |
| RefSeq Size | 2765 bp |
| RefSeq ORF | 951 bp |
| Locus ID | 80381 |
| UniProt ID | Q5ZPR3 |
| Cytogenetics | 15q24.1 |
| Domains | IG, ig, IGc2 |
| Protein Families | Druggable Genome, Transmembrane |
| Protein Pathways | Cell adhesion molecules (CAMs) |
| MW | 33.6 kDa |
| Summary | The protein encoded by this gene belongs to the immunoglobulin superfamily, and thought to participate in the regulation of T-cell-mediated immune response. Studies show that while the transcript of this gene is ubiquitously expressed in normal tissues and solid tumors, the protein is preferentially expressed only in tumor tissues. Additionally, it was observed that the 3' UTR of this transcript contains a target site for miR29 microRNA, and there is an inverse correlation between the expression of this protein and miR29 levels, suggesting regulation of expression of this gene product by miR29. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011] |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_025240" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC215291L1 | Lenti ORF clone of Human CD276 molecule (CD276), transcript variant 2, Myc-DDK-tagged | 10 ug |
$600.00
|
|
| RC215291L2 | Lenti ORF clone of Human CD276 molecule (CD276), transcript variant 2, mGFP tagged | 10 ug |
$600.00
|
|
| RC215291L3 | Lenti ORF clone of Human CD276 molecule (CD276), transcript variant 2, Myc-DDK-tagged | 10 ug |
$600.00
|
|
| RC215291L4 | Lenti ORF clone of Human CD276 molecule (CD276), transcript variant 2, mGFP tagged | 10 ug |
$600.00
|
|
| RG215291 | CD276 (tGFP-tagged) - Human CD276 molecule (CD276), transcript variant 2 | 10 ug |
$489.00
$500.00
|
|
| SC312930 | CD276 (untagged)-Human CD276 molecule (CD276), transcript variant 2 | 10 ug |
$300.00
|
|
Plasmid Map for RC215291
Download sequence for
Cloning scheme for RC215291
Circular map for RC215291
Citations
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
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