Factor V (F5) (NM_000130) Human Tagged ORF Clone

SKU RC215231

F5 (Myc-DDK-tagged)-Human coagulation factor V (proaccelerin, labile factor) (F5)

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: Factor V
• Synonyms: FVL; PCCF; RPRGL1; THPH2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC215231 representing NM_000130; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGTTCCCAGGCTGCCCACGCCTCTGGGTCCTGGTGGTCTTGGGCACCAGCTGGGTAGGCTGGGGGAGCC; AAGGGACAGAAGCGGCACAGCTAAGGCAGTTCTACGTGGCTGCTCAGGGCATCAGTTGGAGCTACCGACC; TGAGCCCACAAACTCAAGTTTGAATCTTTCTGTAACTTCCTTTAAGAAAATTGTCTACAGAGAGTATGAA; CCATATTTTAAGAAAGAAAAACCACAATCTACCATTTCAGGACTTCTTGGGCCTACTTTATATGCTGAAG; TCGGAGACATCATAAAAGTTCACTTTAAAAATAAGGCAGATAAGCCCTTGAGCATCCATCCTCAAGGAAT; TAGGTACAGTAAATTATCAGAAGGTGCTTCTTACCTTGACCACACATTCCCTGCGGAGAAGATGGACGAC; GCTGTGGCTCCAGGCCGAGAATACACCTATGAATGGAGTATCAGTGAGGACAGTGGACCCACCCATGATG; ACCCTCCATGCCTCACACACATCTATTACTCCCATGAAAATCTGATCGAGGATTTCAACTCGGGGCTGAT; TGGGCCCCTGCTTATCTGTAAAAAAGGGACCCTAACTGAGGGTGGGACACAGAAGACGTTTGACAAGCAA; ATCGTGCTACTATTTGCTGTGTTTGATGAAAGCAAGAGCTGGAGCCAGTCATCATCCCTAATGTACACAG; TCAATGGATATGTGAATGGGACAATGCCAGATATAACAGTTTGTGCCCATGACCACATCAGCTGGCATCT; GCTGGGAATGAGCTCGGGGCCAGAATTATTCTCCATTCATTTCAACGGCCAGGTCCTGGAGCAGAACCAT; CATAAGGTCTCAGCCATCACCCTTGTCAGTGCTACATCCACTACCGCAAATATGACTGTGGGCCCAGAGG; GAAAGTGGATCATATCTTCTCTCACCCCAAAACATTTGCAAGCTGGGATGCAGGCTTACATTGACATTAA; AAACTGCCCAAAGAAAACCAGGAATCTTAAGAAAATAACTCGTGAGCAGAGGCGGCACATGAAGAGGTGG; GAATACTTCATTGCTGCAGAGGAAGTCATTTGGGACTATGCACCTGTAATACCAGCGAATATGGACAAAA; AATACAGGTCTCAGCATTTGGATAATTTCTCAAACCAAATTGGAAAACATTATAAGAAAGTTATGTACAC; ACAGTACGAAGATGAGTCCTTCACCAAACATACAGTGAATCCCAATATGAAAGAAGATGGGATTTTGGGT; CCTATTATCAGAGCCCAGGTCAGAGACACACTCAAAATCGTGTTCAAAAATATGGCCAGCCGCCCCTATA; GCATTTACCCTCATGGAGTGACCTTCTCGCCTTATGAAGATGAAGTCAACTCTTCTTTCACCTCAGGCAG; GAACAACACCATGATCAGAGCAGTTCAACCAGGGGAAACCTATACTTATAAGTGGAACATCTTAGAGTTT; GATGAACCCACAGAAAATGATGCCCAGTGCTTAACAAGACCATACTACAGTGACGTGGACATCATGAGAG; ACATCGCCTCTGGGCTAATAGGACTACTTCTAATCTGTAAGAGCAGATCCCTGGACAGGCGAGGAATACA; GAGGGCAGCAGACATCGAACAGCAGGCTGTGTTTGCTGTGTTTGATGAGAACAAAAGCTGGTACCTTGAG; GACAACATCAACAAGTTTTGTGAAAATCCTGATGAGGTGAAACGTGATGACCCCAAGTTTTATGAATCAA; ACATCATGAGCACTATCAATGGCTATGTGCCTGAGAGCATAACTACTCTTGGATTCTGCTTTGATGACAC; TGTCCAGTGGCACTTCTGTAGTGTGGGGACCCAGAATGAAATTTTGACCATCCACTTCACTGGGCACTCA; TTCATCTATGGAAAGAGGCATGAGGACACCTTGACCCTCTTCCCCATGCGTGGAGAATCTGTGACGGTCA; CAATGGATAATGTTGGAACTTGGATGTTAACTTCCATGAATTCTAGTCCAAGAAGCAAAAAGCTGAGGCT; GAAATTCAGGGATGTTAAATGTATCCCAGATGATGATGAAGACTCATATGAGATTTTTGAACCTCCAGAA; TCTACAGTCATGGCTACACGGAAAATGCATGATCGTTTAGAACCTGAAGATGAAGAGAGTGATGCTGACT; ATGATTACCAGAACAGACTGGCTGCAGCATTAGGAATCAGGTCATTCCGAAACTCATCATTGAATCAGGA; AGAAGAAGAGTTCAATCTTACTGCCCTAGCTCTGGAGAATGGCACTGAATTCGTTTCTTCAAACACAGAT; ATAATTGTTGGTTCAAATTATTCTTCCCCAAGTAATATTAGTAAGTTCACTGTCAATAACCTTGCAGAAC; CTCAGAAAGCCCCTTCTCACCAACAAGCCACCACAGCTGGTTCCCCACTGAGACACCTCATTGGCAAGAA; CTCAGTTCTCAATTCTTCCACAGCAGAGCATTCCAGCCCATATTCTGAAGACCCTATAGAGGATCCTCTA; CAGCCAGATGTCACAGGGATACGTCTACTTTCACTTGGTGCTGGAGAATTCAAAAGTCAAGAACATGCTA; AGCATAAGGGACCCAAGGTAGAAAGAGATCAAGCAGCAAAGCACAGGTTCTCCTGGATGAAATTACTAGC; ACATAAAGTTGGGAGACACCTAAGCCAAGACACTGGTTCTCCTTCCGGAATGAGGCCCTGGGAGGACCTT; CCTAGCCAAGACACTGGTTCTCCTTCCAGAATGAGGCCCTGGAAGGACCCTCCTAGTGATCTGTTACTCT; TAAAACAAAGTAACTCATCTAAGATTTTGGTTGGGAGATGGCATTTGGCTTCTGAGAAAGGTAGCTATGA; AATAATCCAAGATACTGATGAAGACACAGCTGTTAACAATTGGCTGATCAGCCCCCAGAATGCCTCACGT; GCTTGGGGAGAAAGCACCCCTCTTGCCAACAAGCCTGGAAAGCAGAGTGGCCACCCAAAGTTTCCTAGAG; TTAGACATAAATCTCTACAAGTAAGACAGGATGGAGGAAAGAGTAGACTGAAGAAAAGCCAGTTTCTCAT; TAAGACACGAAAAAAGAAAAAAGAGAAGCACACACACCATGCTCCTTTATCTCCGAGGACCTTTCACCCT; CTAAGAAGTGAAGCCTACAACACATTTTCAGAAAGAAGACTTAAGCATTCGTTGGTGCTTCATAAATCCA; ATGAAACATCTCTTCCCACAGACCTCAATCAGACATTGCCCTCTATGGATTTTGGCTGGATAGCCTCACT; TCCTGACCATAATCAGAATTCCTCAAATGACACTGGTCAGGCAAGCTGTCCTCCAGGTCTTTATCAGACA; GTGCCCCCAGAGGAACACTATCAAACATTCCCCATTCAAGACCCTGATCAAATGCACTCTACTTCAGACC; CCAGTCACAGATCCTCTTCTCCAGAGCTCAGTGAAATGCTTGAGTATGACCGAAGTCACAAGTCCTTCCC; CACAGATATAAGTCAAATGTCCCCTTCCTCAGAACATGAAGTCTGGCAGACAGTCATCTCTCCAGACCTC; AGCCAGGTGACCCTCTCTCCAGAACTCAGCCAGACAAACCTCTCTCCAGACCTCAGCCACACGACTCTCT; CTCCAGAACTCATTCAGAGAAACCTTTCCCCAGCCCTCGGTCAGATGCCCATTTCTCCAGACCTCAGCCA; TACAACCCTTTCTCCAGACCTCAGCCATACAACCCTTTCTTTAGACCTCAGCCAGACAAACCTCTCTCCA; GAACTCAGTCAGACAAACCTTTCTCCAGCCCTCGGTCAGATGCCCCTTTCTCCAGACCTCAGCCATACAA; CCCTTTCTCTAGACTTCAGCCAGACAAACCTCTCTCCAGAACTCAGCCATATGACTCTCTCTCCAGAACT; CAGTCAGACAAACCTTTCCCCAGCCCTCGGTCAGATGCCCATTTCTCCAGACCTCAGCCATACAACCCTT; TCTCTAGACTTCAGCCAGACAAACCTCTCTCCAGAACTCAGTCAAACAAACCTTTCCCCAGCCCTCGGTC; AGATGCCCCTTTCTCCAGACCCCAGCCATACAACCCTTTCTCTAGACCTCAGCCAGACAAACCTCTCTCC; AGAACTCAGTCAGACAAACCTTTCCCCAGACCTCAGTGAGATGCCCCTCTTTGCAGATCTCAGTCAAATT; CCCCTTACCCCAGACCTCGACCAGATGACACTTTCTCCAGACCTTGGTGAGACAGATCTTTCCCCAAACT; TTGGTCAGATGTCCCTTTCCCCAGACCTCAGCCAGGTGACTCTCTCTCCAGACATCAGTGACACCACCCT; TCTCCCGGATCTCAGCCAGATATCACCTCCTCCAGACCTTGATCAGATATTCTACCCTTCTGAATCTAGT; CAGTCATTGCTTCTTCAAGAATTTAATGAGTCTTTTCCTTATCCAGACCTTGGTCAGATGCCATCTCCTT; CATCTCCTACTCTCAATGATACTTTTCTATCAAAGGAATTTAATCCACTGGTTATAGTGGGCCTCAGTAA; AGATGGTACAGATTACATTGAGATCATTCCAAAGGAAGAGGTCCAGAGCAGTGAAGATGACTATGCTGAA; ATTGATTATGTGCCCTATGATGACCCCTACAAAACTGATGTTAGGACAAACATCAACTCCTCCAGAGATC; CTGACAACATTGCAGCATGGTACCTCCGCAGCAACAATGGAAACAGAAGAAATTATTACATTGCTGCTGA; AGAAATATCCTGGGATTATTCAGAATTTGTACAAAGGGAAACAGATATTGAAGACTCTGATGATATTCCA; GAAGATACCACATATAAGAAAGTAGTTTTTCGAAAGTACCTCGACAGCACTTTTACCAAACGTGATCCTC; GAGGGGAGTATGAAGAGCATCTCGGAATTCTTGGTCCTATTATCAGAGCTGAAGTGGATGATGTTATCCA; AGTTCGTTTTAAAAATTTAGCATCCAGACCGTATTCTCTACATGCCCATGGACTTTCCTATGAAAAATCA; TCAGAGGGAAAGACTTATGAAGATGACTCTCCTGAATGGTTTAAGGAAGATAATGCTGTTCAGCCAAATA; GCAGTTATACCTACGTATGGCATGCCACTGAGCGATCAGGGCCAGAAAGTCCTGGCTCTGCCTGTCGGGC; TTGGGCCTACTACTCAGCTGTGAACCCAGAAAAAGATATTCACTCAGGCTTGATAGGTCCCCTCCTAATC; TGCCAAAAAGGAATACTACATAAGGACAGCAACATGCCTATGGACATGAGAGAATTTGTCTTACTATTTA; TGACCTTTGATGAAAAGAAGAGCTGGTACTATGAAAAGAAGTCCCGAAGTTCTTGGAGACTCACATCCTC; AGAAATGAAAAAATCCCATGAGTTTCACGCCATTAATGGGATGATCTACAGCTTGCCTGGCCTGAAAATG; TATGAGCAAGAGTGGGTGAGGTTACACCTGCTGAACATAGGCGGCTCCCAAGACATTCACGTGGTTCACT; TTCACGGCCAGACCTTGCTGGAAAATGGCAATAAACAGCACCAGTTAGGGGTCTGGCCCCTTCTGCCTGG; TTCATTTAAAACTCTTGAAATGAAGGCATCAAAACCTGGCTGGTGGCTCCTAAACACAGAGGTTGGAGAA; AACCAGAGAGCAGGGATGCAAACGCCATTTCTTATCATGGACAGAGACTGTAGGATGCCAATGGGACTAA; GCACTGGTATCATATCTGATTCACAGATCAAGGCTTCAGAGTTTCTGGGTTACTGGGAGCCCAGATTAGC; AAGATTAAACAATGGTGGATCTTATAATGCTTGGAGTGTAGAAAAACTTGCAGCAGAATTTGCCTCTAAA; CCTTGGATCCAGGTGGACATGCAAAAGGAAGTCATAATCACAGGGATCCAGACCCAAGGTGCCAAACACT; ACCTGAAGTCCTGCTATACCACAGAGTTCTATGTAGCTTACAGTTCCAACCAGATCAACTGGCAGATCTT; CAAAGGGAACAGCACAAGGAATGTGATGTATTTTAATGGCAATTCAGATGCCTCTACAATAAAAGAGAAT; CAGTTTGACCCACCTATTGTGGCTAGATATATTAGGATCTCTCCAACTCGAGCCTATAACAGACCTACCC; TTCGATTGGAACTGCAAGGTTGTGAGGTAAATGGATGTTCCACACCCCTGGGTATGGAAAATGGAAAGAT; AGAAAACAAGCAAATCACAGCTTCTTCGTTTAAGAAATCTTGGTGGGGAGATTACTGGGAACCCTTCCGT; GCCCGTCTGAATGCCCAGGGACGTGTGAATGCCTGGCAAGCCAAGGCAAACAACAATAAGCAGTGGCTAG; AAATTGATCTACTCAAGATCAAGAAGATAACGGCAATTATAACACAGGGCTGCAAGTCTCTGTCCTCTGA; AATGTATGTAAAGAGCTATACCATCCACTACAGTGAGCAGGGAGTGGAATGGAAACCATACAGGCTGAAA; TCCTCCATGGTGGACAAGATTTTTGAAGGAAATACTAATACCAAAGGACATGTGAAGAACTTTTTCAACC; CCCCAATCATTTCCAGGTTTATCCGTGTCATTCCTAAAACATGGAATCAAAGTATTGCACTTCGCCTGGA; ACTCTTTGGCTGTGATATTTAC; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC215231 representing NM_000130; Red=Cloning site Green=Tags(s); ; MFPGCPRLWVLVVLGTSWVGWGSQGTEAAQLRQFYVAAQGISWSYRPEPTNSSLNLSVTSFKKIVYREYE; PYFKKEKPQSTISGLLGPTLYAEVGDIIKVHFKNKADKPLSIHPQGIRYSKLSEGASYLDHTFPAEKMDD; AVAPGREYTYEWSISEDSGPTHDDPPCLTHIYYSHENLIEDFNSGLIGPLLICKKGTLTEGGTQKTFDKQ; IVLLFAVFDESKSWSQSSSLMYTVNGYVNGTMPDITVCAHDHISWHLLGMSSGPELFSIHFNGQVLEQNH; HKVSAITLVSATSTTANMTVGPEGKWIISSLTPKHLQAGMQAYIDIKNCPKKTRNLKKITREQRRHMKRW; EYFIAAEEVIWDYAPVIPANMDKKYRSQHLDNFSNQIGKHYKKVMYTQYEDESFTKHTVNPNMKEDGILG; PIIRAQVRDTLKIVFKNMASRPYSIYPHGVTFSPYEDEVNSSFTSGRNNTMIRAVQPGETYTYKWNILEF; DEPTENDAQCLTRPYYSDVDIMRDIASGLIGLLLICKSRSLDRRGIQRAADIEQQAVFAVFDENKSWYLE; DNINKFCENPDEVKRDDPKFYESNIMSTINGYVPESITTLGFCFDDTVQWHFCSVGTQNEILTIHFTGHS; FIYGKRHEDTLTLFPMRGESVTVTMDNVGTWMLTSMNSSPRSKKLRLKFRDVKCIPDDDEDSYEIFEPPE; STVMATRKMHDRLEPEDEESDADYDYQNRLAAALGIRSFRNSSLNQEEEEFNLTALALENGTEFVSSNTD; IIVGSNYSSPSNISKFTVNNLAEPQKAPSHQQATTAGSPLRHLIGKNSVLNSSTAEHSSPYSEDPIEDPL; QPDVTGIRLLSLGAGEFKSQEHAKHKGPKVERDQAAKHRFSWMKLLAHKVGRHLSQDTGSPSGMRPWEDL; PSQDTGSPSRMRPWKDPPSDLLLLKQSNSSKILVGRWHLASEKGSYEIIQDTDEDTAVNNWLISPQNASR; AWGESTPLANKPGKQSGHPKFPRVRHKSLQVRQDGGKSRLKKSQFLIKTRKKKKEKHTHHAPLSPRTFHP; LRSEAYNTFSERRLKHSLVLHKSNETSLPTDLNQTLPSMDFGWIASLPDHNQNSSNDTGQASCPPGLYQT; VPPEEHYQTFPIQDPDQMHSTSDPSHRSSSPELSEMLEYDRSHKSFPTDISQMSPSSEHEVWQTVISPDL; SQVTLSPELSQTNLSPDLSHTTLSPELIQRNLSPALGQMPISPDLSHTTLSPDLSHTTLSLDLSQTNLSP; ELSQTNLSPALGQMPLSPDLSHTTLSLDFSQTNLSPELSHMTLSPELSQTNLSPALGQMPISPDLSHTTL; SLDFSQTNLSPELSQTNLSPALGQMPLSPDPSHTTLSLDLSQTNLSPELSQTNLSPDLSEMPLFADLSQI; PLTPDLDQMTLSPDLGETDLSPNFGQMSLSPDLSQVTLSPDISDTTLLPDLSQISPPPDLDQIFYPSESS; QSLLLQEFNESFPYPDLGQMPSPSSPTLNDTFLSKEFNPLVIVGLSKDGTDYIEIIPKEEVQSSEDDYAE; IDYVPYDDPYKTDVRTNINSSRDPDNIAAWYLRSNNGNRRNYYIAAEEISWDYSEFVQRETDIEDSDDIP; EDTTYKKVVFRKYLDSTFTKRDPRGEYEEHLGILGPIIRAEVDDVIQVRFKNLASRPYSLHAHGLSYEKS; SEGKTYEDDSPEWFKEDNAVQPNSSYTYVWHATERSGPESPGSACRAWAYYSAVNPEKDIHSGLIGPLLI; CQKGILHKDSNMPMDMREFVLLFMTFDEKKSWYYEKKSRSSWRLTSSEMKKSHEFHAINGMIYSLPGLKM; YEQEWVRLHLLNIGGSQDIHVVHFHGQTLLENGNKQHQLGVWPLLPGSFKTLEMKASKPGWWLLNTEVGE; NQRAGMQTPFLIMDRDCRMPMGLSTGIISDSQIKASEFLGYWEPRLARLNNGGSYNAWSVEKLAAEFASK; PWIQVDMQKEVIITGIQTQGAKHYLKSCYTTEFYVAYSSNQINWQIFKGNSTRNVMYFNGNSDASTIKEN; QFDPPIVARYIRISPTRAYNRPTLRLELQGCEVNGCSTPLGMENGKIENKQITASSFKKSWWGDYWEPFR; ARLNAQGRVNAWQAKANNNKQWLEIDLLKIKKITAIITQGCKSLSSEMYVKSYTIHYSEQGVEWKPYRLK; SSMVDKIFEGNTNTKGHVKNFFNPPIISRFIRVIPKTWNQSIALRLELFGCDIY; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Chromatograms: Chromatograms ; Sequencher program is needed, download here
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_000130
• ORF Size: 6672 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_000130.5
• RefSeq Size: 6914 bp
• RefSeq ORF: 6675 bp
• Locus ID: 2153
• UniProt ID: P12259
• Cytogenetics: 1q24.2
• Domains: Cu-oxidase, F5_F8_type_C
• Protein Families: Druggable Genome
• Protein Pathways: Complement and coagulation cascades
• MW: 251.7 kDa
• Summary: This gene encodes an essential cofactor of the blood coagulation cascade. This factor circulates in plasma, and is converted to the active form by the release of the activation peptide by thrombin during coagulation. This generates a heavy chain and a light chain which are held together by calcium ions. The activated protein is a cofactor that participates with activated coagulation factor X to activate prothrombin to thrombin. Defects in this gene result in either an autosomal recessive hemorrhagic diathesis or an autosomal dominant form of thrombophilia, which is known as activated protein C resistance. [provided by RefSeq, Oct 2008]