FGR (NM_001042747) Human Tagged ORF Clone

SKU RC215016

FGR (Myc-DDK-tagged)-Human Gardner-Rasheed feline sarcoma viral (v-fgr) oncogene homolog (FGR), transcript variant 2

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: FGR
• Synonyms: c-fgr; c-src2; p55-Fgr; p55c-fgr; p58-Fgr; p58c-fgr; SRC2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC215016 ORF sequence; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGGCTGTGTGTTCTGCAAGAAATTGGAGCCGGTGGCCACGGCCAAGGAGGATGCTGGCCTGGAAGGGG; ACTTCAGAAGCTACGGGGCAGCAGACCACTATGGGCCTGACCCCACTAAGGCCCGGCCTGCATCCTCATT; TGCCCACATCCCCAACTACAGCAACTTCTCCTCTCAGGCCATCAACCCTGGCTTCCTTGATAGTGGCACC; ATCAGGGGTGTGTCAGGGATTGGGGTGACCCTGTTCATTGCCCTGTATGACTATGAGGCTCGAACTGAGG; ATGACCTCACCTTCACCAAGGGCGAGAAGTTCCACATCCTGAACAATACTGAAGGTGACTGGTGGGAGGC; TCGGTCTCTCAGCTCCGGAAAAACTGGCTGCATTCCCAGCAACTACGTGGCCCCTGTTGACTCAATCCAA; GCTGAAGAGTGGTACTTTGGAAAGATTGGGAGAAAGGATGCAGAGAGGCAGCTGCTTTCACCAGGCAACC; CCCAGGGGGCCTTTCTCATTCGGGAAAGCGAGACCACCAAAGGTGCCTACTCCCTGTCCATCCGGGACTG; GGATCAGACCAGAGGCGATCATGTGAAGCATTACAAGATCCGCAAACTGGACATGGGCGGCTACTACATC; ACCACACGGGTTCAGTTCAACTCGGTGCAGGAGCTGGTGCAGCACTACATGGAGGTGAATGACGGGCTGT; GCAACCTGCTCATCGCGCCCTGCACCATCATGAAGCCGCAGACGCTGGGCCTGGCCAAGGACGCCTGGGA; GATCAGCCGCAGCTCCATCACGCTGGAGCGCCGGCTGGGCACCGGCTGCTTCGGGGATGTGTGGCTGGGC; ACGTGGAACGGCAGCACTAAGGTGGCGGTGAAGACGCTGAAGCCGGGCACCATGTCCCCGAAGGCCTTCC; TGGAGGAGGCGCAGGTCATGAAGCTGCTGCGGCACGACAAGCTGGTGCAGCTGTACGCCGTGGTGTCGGA; GGAGCCCATCTACATCGTGACCGAGTTCATGTGTCACGGCAGCTTGCTGGATTTTCTCAAGAACCCAGAG; GGCCAGGATTTGAGGCTGCCCCAATTGGTGGACATGGCAGCCCAGGTAGCTGAGGGCATGGCCTACATGG; AACGCATGAACTACATTCACCGCGACCTGAGGGCAGCCAACATCCTGGTTGGGGAGCGGCTGGCGTGCAA; GATCGCAGACTTTGGCTTGGCGCGTCTCATCAAGGACGATGAGTACAACCCCTGCCAAGGTTCCAAGTTC; CCCATCAAGTGGACAGCCCCAGAAGCTGCCCTCTTTGGCAGATTCACCATCAAGTCAGACGTGTGGTCCT; TTGGGATCCTGCTCACTGAGCTCATCACCAAGGGCCGAATCCCCTACCCAGGCATGAATAAACGGGAAGT; GTTGGAACAGGTGGAGCAGGGCTACCACATGCCGTGCCCTCCAGGCTGCCCAGCATCCCTGTACGAGGCC; ATGGAACAGACCTGGCGTCTGGACCCGGAGGAGAGGCCTACCTTCGAGTACCTGCAGTCCTTCCTGGAGG; ACTACTTCACCTCCGCTGAACCACAGTACCAGCCCGGGGATCAGACA; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC215016 protein sequence; Red=Cloning site Green=Tags(s); ; MGCVFCKKLEPVATAKEDAGLEGDFRSYGAADHYGPDPTKARPASSFAHIPNYSNFSSQAINPGFLDSGT; IRGVSGIGVTLFIALYDYEARTEDDLTFTKGEKFHILNNTEGDWWEARSLSSGKTGCIPSNYVAPVDSIQ; AEEWYFGKIGRKDAERQLLSPGNPQGAFLIRESETTKGAYSLSIRDWDQTRGDHVKHYKIRKLDMGGYYI; TTRVQFNSVQELVQHYMEVNDGLCNLLIAPCTIMKPQTLGLAKDAWEISRSSITLERRLGTGCFGDVWLG; TWNGSTKVAVKTLKPGTMSPKAFLEEAQVMKLLRHDKLVQLYAVVSEEPIYIVTEFMCHGSLLDFLKNPE; GQDLRLPQLVDMAAQVAEGMAYMERMNYIHRDLRAANILVGERLACKIADFGLARLIKDDEYNPCQGSKF; PIKWTAPEAALFGRFTIKSDVWSFGILLTELITKGRIPYPGMNKREVLEQVEQGYHMPCPPGCPASLYEA; MEQTWRLDPEERPTFEYLQSFLEDYFTSAEPQYQPGDQT; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Chromatograms: Chromatograms ; Sequencher program is needed, download here
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001042747
• ORF Size: 1587 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001042747.1, NP_001036212.1
• RefSeq Size: 2501 bp
• RefSeq ORF: 1590 bp
• Locus ID: 2268
• UniProt ID: P09769
• Cytogenetics: 1p35.3
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Chemokine signaling pathway
• MW: 59.5 kDa
• Summary: This gene is a member of the Src family of protein tyrosine kinases (PTKs). The encoded protein contains N-terminal sites for myristylation and palmitylation, a PTK domain, and SH2 and SH3 domains which are involved in mediating protein-protein interactions with phosphotyrosine-containing and proline-rich motifs, respectively. The protein localizes to plasma membrane ruffles, and functions as a negative regulator of cell migration and adhesion triggered by the beta-2 integrin signal transduction pathway. Infection with Epstein-Barr virus results in the overexpression of this gene. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Jul 2008]