BSX (NM_001098169) Human Tagged ORF Clone

SKU
RC214365
BSX (Myc-DDK-tagged)-Human brain-specific homeobox (BSX)
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$300.00
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Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol BSX
Synonyms BSX1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC214365 representing NM_001098169
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAATCTCAACTTCACCTCTCCTCTACACCCGGCGTCTTCTCAGAGGCCCACATCCTTCTTCATCGAGG
ACATCCTGCTGCACAAGCCCAAGCCGCTGAGAGAGGTGGCCCCAGACCATTTCGCCAGCTCTCTGGCCTC
TCGGGTGCCTCTGCTAGACTATGGCTACCCCCTCATGCCCACACCCACCCTCTTGGCTCCTCACGCCCAT
CACCCTCTGCATAAGGGAGACCACCATCATCCTTATTTCCTCACCACCTCGGGGATGCCAGTCCCAGCGC
TGTTCCCGCACCCGCAGCACGCGGAGCTGCCGGGGAAGCACTGCCGCCGCCGCAAAGCCCGCACGGTTTT
CTCTGACTCGCAGCTCTCGGGCTTGGAGAAGAGGTTCGAGATCCAGCGCTACCTGTCCACGCCAGAACGA
GTGGAGCTGGCCACGGCCCTCAGCCTGTCCGAGACGCAGGTGAAAACGTGGTTCCAGAACCGGCGGATGA
AGCATAAAAAGCAACTGCGGAAAAGCCAAGACGAACCCAAAGCACCAGACGGGCCAGAAAGCCCCGAGGG
CAGCCCCCGCGGTTCAGAGGCCGCCACCGCCGCCGAGGCTCGGCTGAGCCTGCCCGCCGGTCCCTTCGTG
CTGACCGAGCCAGAGGACGAGGTGGACATTGGAGACGAGGGGGAGCTGGGCTCAGGGCCGCACGTGCTC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC214365 representing NM_001098169
Red=Cloning site Green=Tags(s)

MNLNFTSPLHPASSQRPTSFFIEDILLHKPKPLREVAPDHFASSLASRVPLLDYGYPLMPTPTLLAPHAH
HPLHKGDHHHPYFLTTSGMPVPALFPHPQHAELPGKHCRRRKARTVFSDSQLSGLEKRFEIQRYLSTPER
VELATALSLSETQVKTWFQNRRMKHKKQLRKSQDEPKAPDGPESPEGSPRGSEAATAAEARLSLPAGPFV
LTEPEDEVDIGDEGELGSGPHVL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene Plasmid Map
ACCN NM_001098169
ORF Size 699 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001098169.2
RefSeq Size 702 bp
RefSeq ORF 702 bp
Locus ID 390259
UniProt ID Q3C1V8
Cytogenetics 11q24.1
MW 25.8 kDa
Summary DNA binding protein that function as transcriptional activator. Is essential for normal postnatal growth and nursing. Is an essential factor for neuronal neuropeptide Y and agouti-related peptide function and locomotory behavior in the control of energy balance (By similarity).[UniProtKB/Swiss-Prot Function]
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SKU Description Size Price
RC214365L3 Lenti ORF clone of Human brain-specific homeobox (BSX), Myc-DDK-tagged 10 ug
$600.00
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RG214365 BSX (tGFP-tagged) - Human brain-specific homeobox (BSX) 10 ug
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SC316164 BSX (untagged)-Human brain-specific homeobox (BSX) 10 ug
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Citations

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