SLC22A17 (NM_016609) Human Tagged ORF Clone

SKU RC214220

SLC22A17 (Myc-DDK-tagged)-Human solute carrier family 22, member 17 (SLC22A17), transcript variant 2

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: SLC22A17
• Synonyms: 24p3R; BOCT; BOIT; hBOIT; NGALR; NGALR2; NGALR3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC214220 representing NM_016609; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGCCTCGGACCCCATCTTCACGCTGGCGCCCCCGCTGCATTGCCACTACGGGGCCTTCCCCCCTAATG; CCTCTGGCTGGGAGCAGCCTCCCAATGCCAGCGGCGTCAGCGTCGCCAGCGCTGCCCTAGCAGCCAGCGC; CGCCAGCCGTGTCGCCACCAGTACCGACCCCTCGTGCAGCGGCTTCGCCCCGCCGGACTTCAACCATTGC; CTCAAGGATTGGGACTATAATGGCCTTCCTGTGCTCACCACCAACGCCATCGGCCAGTGGGATCTGGTGT; GTGACCTGGGCTGGCAGGTGATCCTGGAGCAGATCCTCTTCATCTTGGGCTTTGCCTCCGGCTACCTGTT; CCTGGGTTACCCCGCAGACAGATTTGGCCGTCGCGGGATTGTGCTGCTGACCTTGGGGCTGGTGGGCCCC; TGTGGAGTAGGAGGGGCTGCTGCAGGCTCCTCCACAGGCGTCATGGCCCTCCGATTCCTCTTGGGCTTTC; TGCTTGCCGGTGTTGACCTGGGTGTCTACCTGATGCGCCTGGAGCTGTGCGACCCAACCCAGAGGCTTCG; GGTGGCCCTGGCAGGGGAGTTGGTGGGGGTGGGAGGGCACTTCCTGTTCCTGGGCCTGGCCCTTGTCTCT; AAGGATTGGCGATTCCTACAGCGAATGATCACCGCTCCCTGCATCCTCTTCCTGTTTTATGGCTGGCCTG; GTTTGTTCCTGGAGTCCGCACGGTGGCTGATAGTGAAGCGGCAGATTGAGGAGGCTCAGTCTGTGCTGAG; GATCCTGGCTGAGCGAAACCGGCCCCATGGGCAGATGCTGGGGGAGGAGGCCCAGGAGGCCCTGCAGGAC; CTGGAGAATACCTGCCCTCTCCCTGCAACATCCTCCTTTTCCTTTGCTTCCCTCCTCAACTACCGCAACA; TCTGGAAAAATCTGCTTATCCTGGGCTTCACCAACTTCATTGCCCATGCCATTCGCCACTGCTACCAGCC; TGTGGGAGGAGGAGGGAGCCCATCGGACTTCTACCTGTGCTCTCTGCTGGCCAGCGGCACCGCAGCCCTG; GCCTGTGTCTTCCTGGGGGTCACCGTGGACCGATTTGGCCGCCGGGGCATCCTTCTTCTCTCCATGACCC; TTACCGGCATTGCTTCCCTGGTCCTGCTGGGCCTGTGGGATTATCTGAACGAGGCTGCCATCACCACTTT; CTCTGTCCTTGGGCTCTTCTCCTCCCAAGCTGCCGCCATCCTCAGCACCCTCCTTGCTGCTGAGGTCATC; CCCACCACTGTCCGGGGCCGTGGCCTGGGCCTGATCATGGCTCTAGGGGCGCTTGGAGGACTGAGCGGCC; CGGCCCAGCGCCTCCACATGGGCCATGGAGCCTTCCTGCAGCACGTGGTGCTGGCGGCCTGCGCCCTCCT; CTGCATTCTCAGCATTATGCTGCTGCCGGAGACCAAGCGCAAGCTCCTGCCCGAGGTGCTCCGGGACGGG; GAGCTGTGTCGCCGGCCTTCCCTGCTGCGGCAGCCACCCCCTACCCGCTGTGACCACGTCCCGCTGCTTG; CCACCCCCAACCCTGCCCTC; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC214220 representing NM_016609; Red=Cloning site Green=Tags(s); ; MASDPIFTLAPPLHCHYGAFPPNASGWEQPPNASGVSVASAALAASAASRVATSTDPSCSGFAPPDFNHC; LKDWDYNGLPVLTTNAIGQWDLVCDLGWQVILEQILFILGFASGYLFLGYPADRFGRRGIVLLTLGLVGP; CGVGGAAAGSSTGVMALRFLLGFLLAGVDLGVYLMRLELCDPTQRLRVALAGELVGVGGHFLFLGLALVS; KDWRFLQRMITAPCILFLFYGWPGLFLESARWLIVKRQIEEAQSVLRILAERNRPHGQMLGEEAQEALQD; LENTCPLPATSSFSFASLLNYRNIWKNLLILGFTNFIAHAIRHCYQPVGGGGSPSDFYLCSLLASGTAAL; ACVFLGVTVDRFGRRGILLLSMTLTGIASLVLLGLWDYLNEAAITTFSVLGLFSSQAAAILSTLLAAEVI; PTTVRGRGLGLIMALGALGGLSGPAQRLHMGHGAFLQHVVLAACALLCILSIMLLPETKRKLLPEVLRDG; ELCRRPSLLRQPPPTRCDHVPLLATPNPAL; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Chromatograms: Chromatograms ; Sequencher program is needed, download here
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_016609
• ORF Size: 1560 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_016609.4, NP_057693.3
• RefSeq Size: 2344 bp
• RefSeq ORF: 1563 bp
• Locus ID: 51310
• UniProt ID: Q8WUG5
• Cytogenetics: 14q11.2
• Protein Families: Druggable Genome, Transmembrane
• MW: 55.5 kDa
• Summary: Cell surface receptor for LCN2 (24p3) that plays a key role in iron homeostasis and transport. Able to bind iron-bound LCN2 (holo-24p3), followed by internalization of holo-24p3 and release of iron, thereby increasing intracellular iron concentration and leading to inhibition of apoptosis. Also binds iron-free LCN2 (apo-24p3), followed by internalization of apo-24p3 and its association with an intracellular siderophore, leading to iron chelation and iron transfer to the extracellular medium, thereby reducing intracellular iron concentration and resulting in apoptosis (By similarity).[UniProtKB/Swiss-Prot Function]