Myosin (MYH2) (NM_017534) Human Tagged ORF Clone

SKU RC213749

MYH2 (Myc-DDK-tagged)-Human myosin, heavy chain 2, skeletal muscle, adult (MYH2), transcript variant 1

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: Myosin
• Synonyms: IBM3; MYH2A; MYHas8; MyHC-2A; MyHC-IIa; MYHSA2; MYPOP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC213749 representing NM_017534; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGAGTTCAGACTCAGAATTGGCTGTTTTTGGGGAGGCTGCTCCTTTCCTCCGAAAGTCTGAAAGGGAGC; GCATTGAGGCCCAGAATAGGCCCTTTGATGCCAAAACATCTGTCTTTGTGGCGGAGCCCAAAGAATCCTT; TGTCAAAGGGACCATCCAGAGCAGAGAAGGAGGAAAAGTGACGGTGAAGACTGAGGGAGGAGCGACTCTG; ACAGTGAAGGATGATCAGGTCTTCCCCATGAACCCTCCCAAATATGACAAGATCGAGGATATGGCCATGA; TGACTCATCTGCATGAGCCTGCTGTGCTGTACAACCTCAAAGAACGTTATGCAGCCTGGATGATCTACAC; CTATTCAGGTCTCTTCTGTGTCACTGTCAACCCCTACAAGTGGCTGCCTGTGTATAAGCCCGAGGTGGTG; ACAGCCTACCGAGGCAAAAAGCGCCAGGAGGCCCCGCCCCACATCTTCTCCATCTCTGACAACGCCTATC; AGTTCATGCTGACTGACCGAGAGAATCAGTCAATCCTGATCACTGGAGAATCTGGTGCAGGGAAGACTGT; GAACACCAAGCGTGTCATCCAGTACTTTGCAACAATTGCAGTTACTGGTGAGAAGAAGAAGGAAGAAATT; ACTTCTGGCAAAATACAGGGGACTCTGGAAGATCAAATCATCAGTGCCAACCCCCTACTGGAGGCCTTTG; GCAACGCCAAGACCGTGAGGAATGACAACTCCTCTCGCTTTGGTAAATTCATCAGAATCCACTTTGGCAC; TACTGGAAAACTGGCATCTGCTGATATTGAAACATATCTGCTAGAGAAGTCTAGAGTTGTTTTCCAGCTT; AAGGCTGAGAGAAGTTATCATATTTTTTACCAGATTACATCGAATAAGAAACCAGAACTTATTGAAATGC; TTCTGATTACCACGAACCCATATGATTACCCATTTGTCAGTCAAGGGGAGATCAGTGTGGCCAGCATCGA; TGATCAGGAAGAACTGATGGCCACAGATAGTGCTATTGATATTTTGGGCTTTACTAATGAAGAAAAGGTC; TCCATTTACAAGCTCACGGGGGCTGTGATGCATTATGGGAACCTAAAATTTAAGCAAAAGCAGCGTGAGG; AGCAAGCAGAGCCAGATGGCACAGAAGTTGCTGACAAGGCGGCCTACCTCCAGAGTCTGAACTCTGCAGA; TCTGCTCAAAGCTCTCTGCTACCCCAGGGTCAAGGTCGGCAATGAGTATGTCACCAAAGGCCAGACTGTA; GAACAGGTGTCCAACGCAGTAGGTGCTCTGGCCAAAGCCGTCTACGAGAAGATGTTCCTGTGGATGGTTG; CCCGCATCAACCAGCAGCTGGACACCAAGCAGCCCAGGCAGTACTTCATCGGGGTCTTGGACATTGCTGG; TTTTGAGATTTTTGATTTCAACAGCCTGGAGCAGCTGTGCATCAACTTCACCAATGAGAAACTGCAACAG; TTTTTCAACCACCACATGTTCGTGCTGGAGCAGGAGGAGTACAAGAAGGAAGGCATCGAGTGGACGTTCA; TCGACTTCGGGATGGACCTGGCTGCCTGCATCGAGCTCATCGAGAAGCCTATGGGCATCTTCTCCATCCT; GGAAGAGGAGTGCATGTTCCCTAAGGCAACAGACACCTCCTTCAAGAACAAGCTGTATGACCAGCACCTG; GGCAAGTCTGCCAACTTCCAGAAGCCCAAGGTGGTCAAAGGCAAGGCCGAGGCCCACTTCGCTCTGATTC; ACTATGCTGGTGTTGTGGACTACAACATTACTGGCTGGCTGGAGAAGAACAAGGACCCCCTGAATGAGAC; CGTGGTTGGACTGTACCAGAAGTCTGCAATGAAAACTCTAGCTCAGCTCTTCTCTGGGGCTCAAACTGCT; GAAGGAGAGGGAGCTGGTGGAGGGGCCAAGAAAGGTGGTAAGAAGAAGGGCTCTTCTTTCCAGACAGTGT; CTGCCCTTTTCAGAGAGAATTTGAACAAGCTGATGACCAACCTCAGGAGTACCCATCCTCACTTTGTGAG; GTGTATCATCCCCAATGAGACAAAAACTCCTGGTGCCATGGAGCATGAGCTTGTCCTCCACCAGCTGAGG; TGTAACGGTGTGCTGGAAGGCATCCGCATCTGTAGGAAAGGATTTCCAAGCAGAATCCTTTATGCAGACT; TCAAACAGAGATACAAGGTATTAAATGCAAGTGCAATCCCTGAAGGGCAATTCATTGATAGCAAGAAGGC; CTCTGAGAAGCTCCTTGCATCCATCGACATTGACCACACCCAGTATAAATTTGGGCACACCAAGGTCTTT; TTCAAAGCTGGTCTTCTGGGGCTCCTAGAGGAGATGCGAGATGACAAGCTGGCCCAGCTGATTACCCGAA; CCCAGGCCAGGTGCAGAGGGTTCTTGGCAAGAGTGGAGTACCAGAGGATGGTGGAGAGAAGGGAGGCCAT; CTTCTGTATCCAGTACAATATCAGATCCTTCATGAATGTCAAGCACTGGCCCTGGATGAAACTCTTCTTC; AAGATCAAGCCTCTGTTGAAGAGTGCAGAAACTGAGAAGGAGATGGCCACCATGAAGGAAGAATTTCAGA; AAATTAAAGACGAACTTGCCAAGTCAGAGGCAAAAAGGAAGGAACTGGAAGAAAAGATGGTGACGCTGTT; GAAAGAAAAAAATGACTTGCAGCTCCAAGTTCAGGCTGAAGCCGAAGGCTTGGCTGATGCAGAGGAAAGG; TGTGACCAGCTAATCAAAACCAAAATCCAGCTAGAAGCCAAAATCAAAGAGGTGACTGAGAGAGCTGAGG; ATGAGGAAGAGATCAATGCTGAGCTGACAGCCAAGAAGAGGAAACTGGAGGATGAATGTTCAGAACTCAA; GAAAGACATTGATGACCTTGAGCTGACACTGGCCAAGGTTGAGAAGGAGAAACATGCCACAGAAAACAAG; GTGAAAAACCTCACAGAAGAGATGGCAGGTCTGGATGAAACCATTGCTAAGCTGACCAAGGAGAAGAAGG; CTCTCCAGGAGGCCCACCAGCAGACCCTGGATGACCTGCAGGCAGAGGAGGACAAAGTCAACACCCTGAC; CAAAGCTAAAATCAAACTTGAACAACAAGTGGATGATCTTGAAGGGTCCTTGGAGCAAGAAAAGAAACTT; CGCATGGACCTAGAAAGGGCTAAGAGGAAACTTGAGGGTGACTTGAAGTTGGCCCAAGAATCCATAATGG; ACATTGAAAATGAGAAACAGCAACTTGATGAAAAGCTCAAAAAGAAAGAGTTTGAAATCAGCAATCTGCA; AAGCAAGATTGAAGATGAACAGGCACTTGGCATTCAATTGCAGAAGAAAATTAAAGAATTGCAAGCCCGC; ATTGAGGAGCTGGAGGAGGAAATCGAGGCAGAGCGGGCCTCCCGGGCCAAAGCAGAGAAGCAGCGCTCTG; ACCTCTCCCGGGAGCTGGAGGAGATCAGCGAGAGGCTGGAAGAAGCCGGTGGGGCCACTTCAGCCCAGAT; TGAGATGAACAAGAAGCGGGAGGCTGAGTTCCAGAAAATGCGCAGGGACCTGGAGGAGGCCACCCTACAG; CATGAAGCCACAGCGGCCACCCTGAGGAAGAAGCATGCAGATAGTGTGGCCGAGCTTGGGGAGCAGATTG; ACAACCTGCAGCGAGTGAAGCAGAAGCTGGAGAAGGAGAAGAGTGAGATGAAGATGGAGATTGATGACCT; TGCTAGTAATGTAGAAACGGTCTCCAAAGCCAAGGGAAACCTAGAGAAAATGTGCCGGACTCTAGAGGAC; CAACTGAGTGAACTGAAATCAAAGGAAGAGGAGCAGCAGCGGCTGATCAATGACCTGACTGCGCAGAGGG; GGCGCCTGCAGACTGAATCTGGTGAGTTTTCACGCCAGCTTGATGAAAAGGAAGCTCTGGTGTCTCAGTT; ATCAAGAGGCAAACAAGCCTTTACTCAACAGATTGAAGAATTAAAGAGGCAACTTGAAGAGGAGATAAAA; GCCAAGAACGCCCTGGCGCATGCCCTGCAGTCTTCCCGCCACGACTGTGACCTGCTGCGGGAACAGTATG; AGGAGGAGCAGGAATCCAAGGCCGAGCTGCAGAGAGCACTGTCCAAGGCCAACACCGAGGTTGCCCAATG; GAGGACCAAATACGAGACGGACGCCATCCAGCGCACAGAGGAGCTGGAGGAGGCCAAGAAGAAGCTGGCC; CAGCGGCTGCAGGCAGCTGAGGAACATGTAGAAGCTGTGAACGCCAAATGTGCTTCCCTCGAAAAGACGA; AGCAGCGGCTGCAGAATGAGGTCGAGGACCTCATGCTTGATGTGGAGAGGACAAATGCCGCCTGTGCCGC; CCTTGACAAAAAGCAAAGGAACTTCGATAAGATCCTGGCAGAATGGAAACAGAAATGTGAGGAAACGCAT; GCTGAGCTTGAGGCCTCCCAGAAGGAGGCCCGTTCCCTTGGCACTGAGCTGTTCAAGATAAAGAATGCCT; ATGAGGAATCTTTGGATCAGCTAGAAACCCTGAAGCGAGAGAACAAAAACTTACAGCAGGAGATTTCTGA; CCTCACGGAACAGATTGCAGAAGGAGGGAAACGTATCCATGAACTGGAGAAAATAAAGAAACAAGTGGAA; CAAGAAAAGTGTGAACTTCAGGCTGCTTTAGAAGAAGCAGAGGCATCTCTTGAACATGAAGAGGGAAAGA; TCCTGCGCATCCAGCTTGAGTTGAACCAAGTCAAGTCTGAGGTTGATAGGAAAATTGCTGAAAAAGATGA; GGAAATTGACCAGCTGAAGAGAAACCACATTAGAATCGTGGAGTCCATGCAGAGCACGCTGGATGCTGAG; ATCAGGAGTAGGAATGATGCCATTAGGCTCAAGAAGAAGATGGAGGGAGACCTCAATGAAATGGAAATCC; AGCTGAACCATGCCAACCGCATGGCTGCTGAGGCCCTGAGGAACTACAGGAACACCCAAGGCATCCTCAA; GGATACCCAGATCCACCTGGATGATGCTCTCCGGAGCCAGGAGGACCTGAAGGAACAGCTGGCCATGGTG; GAGCGCAGAGCCAACCTGCTGCAGGCTGAGATCGAGGAGCTGCGGGCCACTCTGGAACAGACAGAGAGGA; GCAGAAAAATCGCAGAACAGGAGCTCCTGGATGCCAGTGAGCGTGTTCAGCTACTGCACACCCAGAACAC; CAGCCTGATCAACACCAAGAAGAAGCTGGAGACAGATATTTCCCAAATGCAAGGAGAGATGGAGGACATT; CTCCAGGAAGCCCGCAATGCAGAAGAAAAGGCCAAGAAGGCCATCACTGATGCCGCCATGATGGCTGAGG; AGCTGAAGAAGGAGCAGGACACCAGCGCCCACCTGGAGCGGATGAAGAAGAACATGGAGCAGACCGTGAA; GGATCTGCAGCTCCGTCTGGATGAGGCTGAGCAGCTGGCCCTGAAGGGTGGGAAGAAGCAGATCCAGAAA; CTGGAGGCCAGGGTACGGGAGCTGGAAGGAGAGGTTGAGAGTGAGCAAAAGCGTAATGCTGAGGCTGTCA; AAGGTCTGCGCAAACATGAGAGGCGAGTGAAGGAACTCACTTACCAGACGGAAGAAGATAGAAAGAATAT; TCTCAGGCTTCAAGATTTGGTAGATAAACTTCAGGCAAAAGTGAAATCTTATAAGAGACAAGCTGAGGAG; GCTGAGGAACAATCCAACACCAATCTAGCTAAATTCCGCAAGCTCCAGCATGAGCTGGAGGAGGCCGAGG; AACGGGCTGACATTGCTGAGTCCCAGGTGAACAAACTGCGGGTGAAGAGCCGGGAGGTTCACACAAAAGT; CATAAGTGAAGAG; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC213749 representing NM_017534; Red=Cloning site Green=Tags(s); ; MSSDSELAVFGEAAPFLRKSERERIEAQNRPFDAKTSVFVAEPKESFVKGTIQSREGGKVTVKTEGGATL; TVKDDQVFPMNPPKYDKIEDMAMMTHLHEPAVLYNLKERYAAWMIYTYSGLFCVTVNPYKWLPVYKPEVV; TAYRGKKRQEAPPHIFSISDNAYQFMLTDRENQSILITGESGAGKTVNTKRVIQYFATIAVTGEKKKEEI; TSGKIQGTLEDQIISANPLLEAFGNAKTVRNDNSSRFGKFIRIHFGTTGKLASADIETYLLEKSRVVFQL; KAERSYHIFYQITSNKKPELIEMLLITTNPYDYPFVSQGEISVASIDDQEELMATDSAIDILGFTNEEKV; SIYKLTGAVMHYGNLKFKQKQREEQAEPDGTEVADKAAYLQSLNSADLLKALCYPRVKVGNEYVTKGQTV; EQVSNAVGALAKAVYEKMFLWMVARINQQLDTKQPRQYFIGVLDIAGFEIFDFNSLEQLCINFTNEKLQQ; FFNHHMFVLEQEEYKKEGIEWTFIDFGMDLAACIELIEKPMGIFSILEEECMFPKATDTSFKNKLYDQHL; GKSANFQKPKVVKGKAEAHFALIHYAGVVDYNITGWLEKNKDPLNETVVGLYQKSAMKTLAQLFSGAQTA; EGEGAGGGAKKGGKKKGSSFQTVSALFRENLNKLMTNLRSTHPHFVRCIIPNETKTPGAMEHELVLHQLR; CNGVLEGIRICRKGFPSRILYADFKQRYKVLNASAIPEGQFIDSKKASEKLLASIDIDHTQYKFGHTKVF; FKAGLLGLLEEMRDDKLAQLITRTQARCRGFLARVEYQRMVERREAIFCIQYNIRSFMNVKHWPWMKLFF; KIKPLLKSAETEKEMATMKEEFQKIKDELAKSEAKRKELEEKMVTLLKEKNDLQLQVQAEAEGLADAEER; CDQLIKTKIQLEAKIKEVTERAEDEEEINAELTAKKRKLEDECSELKKDIDDLELTLAKVEKEKHATENK; VKNLTEEMAGLDETIAKLTKEKKALQEAHQQTLDDLQAEEDKVNTLTKAKIKLEQQVDDLEGSLEQEKKL; RMDLERAKRKLEGDLKLAQESIMDIENEKQQLDEKLKKKEFEISNLQSKIEDEQALGIQLQKKIKELQAR; IEELEEEIEAERASRAKAEKQRSDLSRELEEISERLEEAGGATSAQIEMNKKREAEFQKMRRDLEEATLQ; HEATAATLRKKHADSVAELGEQIDNLQRVKQKLEKEKSEMKMEIDDLASNVETVSKAKGNLEKMCRTLED; QLSELKSKEEEQQRLINDLTAQRGRLQTESGEFSRQLDEKEALVSQLSRGKQAFTQQIEELKRQLEEEIK; AKNALAHALQSSRHDCDLLREQYEEEQESKAELQRALSKANTEVAQWRTKYETDAIQRTEELEEAKKKLA; QRLQAAEEHVEAVNAKCASLEKTKQRLQNEVEDLMLDVERTNAACAALDKKQRNFDKILAEWKQKCEETH; AELEASQKEARSLGTELFKIKNAYEESLDQLETLKRENKNLQQEISDLTEQIAEGGKRIHELEKIKKQVE; QEKCELQAALEEAEASLEHEEGKILRIQLELNQVKSEVDRKIAEKDEEIDQLKRNHIRIVESMQSTLDAE; IRSRNDAIRLKKKMEGDLNEMEIQLNHANRMAAEALRNYRNTQGILKDTQIHLDDALRSQEDLKEQLAMV; ERRANLLQAEIEELRATLEQTERSRKIAEQELLDASERVQLLHTQNTSLINTKKKLETDISQMQGEMEDI; LQEARNAEEKAKKAITDAAMMAEELKKEQDTSAHLERMKKNMEQTVKDLQLRLDEAEQLALKGGKKQIQK; LEARVRELEGEVESEQKRNAEAVKGLRKHERRVKELTYQTEEDRKNILRLQDLVDKLQAKVKSYKRQAEE; AEEQSNTNLAKFRKLQHELEEAEERADIAESQVNKLRVKSREVHTKVISEE; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Chromatograms: Chromatograms ; Sequencher program is needed, download here
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_017534
• ORF Size: 5823 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_017534.6
• RefSeq Size: 6062 bp
• RefSeq ORF: 5826 bp
• Locus ID: 4620
• UniProt ID: Q9UKX2
• Cytogenetics: 17p13.1
• Protein Pathways: Tight junction, Viral myocarditis
• MW: 222.9 kDa
• Summary: Myosins are actin-based motor proteins that function in the generation of mechanical force in eukaryotic cells. Muscle myosins are heterohexamers composed of 2 myosin heavy chains and 2 pairs of nonidentical myosin light chains. This gene encodes a member of the class II or conventional myosin heavy chains, and functions in skeletal muscle contraction. This gene is found in a cluster of myosin heavy chain genes on chromosome 17. A mutation in this gene results in inclusion body myopathy-3. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Sep 2009]