Zonula occludens protein 3 (TJP3) (NM_014428) Human Tagged ORF Clone

SKU RC213399

TJP3 (Myc-DDK-tagged)-Human tight junction protein 3 (zona occludens 3) (TJP3)

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: Zonula occludens protein 3
• Synonyms: ZO-3; ZO3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC213399 representing NM_014428; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGAACCTGTGTGGCCTCATGCCCATCTTCCCCGCTCCCCTCGACCAGGTGGCTGACATGGAGGAGCTGA; CCATCTGGGAACAGCACACGGCCACACTGTCCAAGGACCCCCGCCGGGGCTTTGGCATTGCGATCTCTGG; AGGCCGAGACCGGCCCGGTGGATCCATGGTTGTATCTGACGTGGTACCTGGAGGGCCGGCGGAGGGCAGG; CTACAGACAGGCGACCACATCGTCATGGTGAACGGGGTTTCCATGGAGAATGCCACCTCCGCGTTTGCCA; TTCAGATACTCAAGACCTGCACCAAGATGGCCAACATCACAGTGAAACGTCCCCGGAGGATCCACCTGCC; CGCCACCAAAGCCAGCCCCTCCAGCCCAGGGCGCCAGGACTCGGATGAAGACGATGGGCCCCAGCGGGTG; GAGGAGGTGGACCAGGGCCGGGGCTATGACGGCGACTCATCCAGTGGCTCCGGCCGCTCCTGGGACGAGC; GCTCCCGCCGGCCGAGGCCTGGTCGCCGGGGCCGGGCCGGCAGCCATGGGCGTAGGAGCCCAGGTGGTGG; CTCTGAGGCCAACGGGCTGGCCCTGGTGTCCGGCTTTAAGCGGCTGCCACGGCAGGACGTGCAGATGAAG; CCTGTGAAGTCAGTGCTGGTGAAGAGGAGAGACAGCGAAGAGTTTGGCGTCAAGCTGGGCAGTCAGATCT; TCATCAAGCACATTACAGATTCGGGCCTGGCTGCCCGGCACCGTGGGCTGCAGGAAGGAGATCTCATTCT; ACAGATCAACGGGGTGTCTAGCCAGAACCTGTCACTGAACGACACCCGGCGACTGATTGAGAAGTCAGAA; GGGAAGCTAAGCCTGCTGGTGCTGAGAGATCGTGGGCAGTTCCTGGTGAACATTCCGCCTGCTGTCAGTG; ACAGCGACAGCTCGCCATTGGAGGAAGGCGTGACCATGGCTGATGAGATGTCCTCTCCCCCTGCAGACAT; CTCGGACCTCGCCTCGGAGCTATCGCAGGCACCACCATCCCACATCCCACCACCACCCCGGCATGCTCAG; CGGAGCCCCGAGGCCAGCCAGACCGACTCTCCCGTGGAGAGTCCCCGGCTTCGGCGGGAAAGTTCAGTAG; ATTCCAGAACCATCTCGGAACCAGATGAGCAACGGTCAGAGTTGCCCAGGGAAAGCAGCTATGACATCTA; CAGAGTGCCCAGCAGTCAGAGCATGGAGGATCGTGGGTACAGCCCCGACACGCGTGTGGTCCGCTTCCTC; AAGGGCAAGAGCATCGGGCTGCGGCTGGCAGGGGGCAATGACGTGGGCATCTTCGTGTCCGGGGTGCAGG; CGGGCAGCCCGGCCGACGGGCAGGGCATCCAGGAGGGAGATCAGATTCTGCAGGTGAATGACGTGCCATT; CCAGAACCTGACACGGGAGGAGGCAGTGCAGTTCCTGCTGGGGCTGCCACCAGGCGAGGAGATGGAGCTG; GTGACGCAGAGGAAGCAGGACATTTTCTGGAAAATGGTGCAGTCCCGCGTGGGTGACTCCTTCTACATCC; GCACTCACTTTGAGCTGGAGCCCAGTCCACCGTCTGGCCTGGGCTTCACCCGTGGCGACGTCTTCCACGT; GCTGGACACGCTGCACCCCGGCCCCGGGCAGAGCCACGCACGAGGAGGCCACTGGCTGGCGGTGCGCATG; GGTCGTGACCTGCGGGAGCAAGAGCGGGGCATCATTCCCAACCAGAGCAGGGCGGAGCAGCTGGCCAGCC; TGGAAGCTGCCCAGAGGGCCGTGGGAGTCGGGCCCGGCTCCTCCGCGGGCTCCAATGCTCGGGCCGAGTT; CTGGCGGCTGCGGGGTCTTCGTCGAGGAGCCAAGAAGACCACTCAGCGGAGCCGTGAGGACCTCTCAGCT; CTGACCCGACAGGGCCGCTACCCGCCCTACGAACGAGTGGTGTTGCGAGAAGCCAGTTTCAAGCGCCCGG; TAGTGATCCTGGGACCCGTGGCCGACATTGCTATGCAGAAGTTGACTGCTGAGATGCCTGACCAGTTTGA; AATCGCAGAGACTGTGTCCAGGACCGACAGCCCCTCCAAGATCATCAAACTAGACACCGTGCGGGTGATT; GCAGAAAAAGACAAGCATGCGCTCCTGGATGTGACCCCCTCCGCCATCGAGCGCCTCAACTATGTGCAGT; ACTACCCCATTGTGGTCTTCTTCATCCCCGAGAGCCGGCCGGCCCTCAAGGCACTGCGCCAGTGGCTGGC; GCCTGCCTCCCGCCGCAGCACCCGTCGCCTCTACGCACAAGCCCAGAAGCTGCGAAAACACAGCAGCCAC; CTCTTCACAGCCACCATCCCTCTGAATGGCACGAGTGACACCTGGTACCAGGAGCTCAAGGCCATCATTC; GAGAGCAGCAGACGCGGCCCATCTGGACGGCGGAAGATCAGCTGGATGGCTCCTTGGAGGACAACCTAGA; CCTCCCTCACCACGGCCTGGCCGACAGCTCCGCTGACCTCAGCTGCGACAGCCGCGTTAACAGCGACTAC; GAGACGGACGGCGAGGGCGGCGCGTACACGGATGGCGAGGGCTACACAGACGGCGAGGGGGGGCCCTACA; CGGATGTGGATGATGAGCCCCCGGCTCCAGCCCTGGCCCGGTCCTCGGAGCCCGTGCAGGCAGATGAGTC; CCAGAGCCCGAGGGATCGTGGGAGAATCTCGGCTCATCAGGGGGCCCAGGTGGACAGCCGCCACCCCCAG; GGACAGTGGCGACAGGACAGCATGCGAACCTATGAACGGGAAGCCCTGAAGAAAAAGTTTATGCGAGTAC; ATGATGCGGAGTCCTCCGATGAAGACGGCTATGACTGGGGTCCGGCCACTGACCTG; ; AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC; TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC213399 representing NM_014428; Red=Cloning site Green=Tags(s); ; MNLCGLMPIFPAPLDQVADMEELTIWEQHTATLSKDPRRGFGIAISGGRDRPGGSMVVSDVVPGGPAEGR; LQTGDHIVMVNGVSMENATSAFAIQILKTCTKMANITVKRPRRIHLPATKASPSSPGRQDSDEDDGPQRV; EEVDQGRGYDGDSSSGSGRSWDERSRRPRPGRRGRAGSHGRRSPGGGSEANGLALVSGFKRLPRQDVQMK; PVKSVLVKRRDSEEFGVKLGSQIFIKHITDSGLAARHRGLQEGDLILQINGVSSQNLSLNDTRRLIEKSE; GKLSLLVLRDRGQFLVNIPPAVSDSDSSPLEEGVTMADEMSSPPADISDLASELSQAPPSHIPPPPRHAQ; RSPEASQTDSPVESPRLRRESSVDSRTISEPDEQRSELPRESSYDIYRVPSSQSMEDRGYSPDTRVVRFL; KGKSIGLRLAGGNDVGIFVSGVQAGSPADGQGIQEGDQILQVNDVPFQNLTREEAVQFLLGLPPGEEMEL; VTQRKQDIFWKMVQSRVGDSFYIRTHFELEPSPPSGLGFTRGDVFHVLDTLHPGPGQSHARGGHWLAVRM; GRDLREQERGIIPNQSRAEQLASLEAAQRAVGVGPGSSAGSNARAEFWRLRGLRRGAKKTTQRSREDLSA; LTRQGRYPPYERVVLREASFKRPVVILGPVADIAMQKLTAEMPDQFEIAETVSRTDSPSKIIKLDTVRVI; AEKDKHALLDVTPSAIERLNYVQYYPIVVFFIPESRPALKALRQWLAPASRRSTRRLYAQAQKLRKHSSH; LFTATIPLNGTSDTWYQELKAIIREQQTRPIWTAEDQLDGSLEDNLDLPHHGLADSSADLSCDSRVNSDY; ETDGEGGAYTDGEGYTDGEGGPYTDVDDEPPAPALARSSEPVQADESQSPRDRGRISAHQGAQVDSRHPQ; GQWRQDSMRTYEREALKKKFMRVHDAESSDEDGYDWGPATDL; ; SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Chromatograms: Chromatograms ; Sequencher program is needed, download here
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_014428
• ORF Size: 2856 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_014428.1, NP_055243.1
• RefSeq Size: 2859 bp
• RefSeq ORF: 2858 bp
• Locus ID: 27134
• Cytogenetics: 19p13.3
• Protein Pathways: Tight junction
• MW: 104.6 kDa
• Summary: The protein encoded by this gene is a member of the membrane-associated guanylate kinase-like (MAGUK) protein family which is characterized by members having multiple PDZ domains, a single SH3 domain, and a single guanylate kinase-like (GUK)-domain. In addition, members of the zonula occludens protein subfamily have an acidic domain, a basic arginine-rich region, and a proline-rich domain. The protein encoded by this gene plays a role in the linkage between the actin cytoskeleton and tight-junctions and also sequesters cyclin D1 at tight junctions during mitosis. Alternative splicing results in multiple transcript variants encoding distinct isoforms. This gene has a partial pseudogene on chromosome 1. [provided by RefSeq, May 2012]