MYPN (NM_032578) Human Tagged ORF Clone

SKU RC213189

MYPN (Myc-DDK-tagged)-Human myopalladin (MYPN)

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: MYPN
• Synonyms: CMD1DD; CMH22; MYOP; NEM11; RCM4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC213189 representing NM_032578; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGCAAGACGACAGCATAGAAGCTTCTACTTCCATATCTCAGCTTCTAAGAGAGAGCTATTTAGCTGAAA; CCAGACATCGGGGAAACAATGAGAGGAGTCGAGCGGAGCCCTCCTCCAACCCTTGCCATTTCGGCAGTCC; TTCTGGGGCCGCTGAAGGAGGCGGAGGCCAAGATGACCTTCCAGATCTTTCAGCCTTTCTGAGCCAAGAA; GAATTAGACGAAAGTGTCAATTTGGCAAGACTGGCCATCAATTACGACCCTTTGGAGAAGGCAGATGAAA; CTCAAGCTAGAAAACGACTTTCTCCTGATCAGATGAAACACTCACCTAATTTAAGTTTTGAGCCTAACTT; CTGCCAGGATAACCCTCGAAGTCCCACCAGCTCTAAAGAAAGCCCCCAGGAGGCAAAAAGGCCACAGTAT; TGTTCTGAAACCCAGTCCAAAAAAGTATTTTTAAATAAGGCTGCCGACTTCATTGAAGAGCTATCCTCCC; TTTTCAAATCCCACAGCTCCAAAAGGATTAGACCTCGTGCCTGCAAAAACCACAAGAGTAAACTGGAATC; TCAAAACAAAGTTATGCAGGAAAACAGCTCCAGTTTCTCAGATCTGTCAGAAAGACGAGAAAGATCTTCT; GTTCCCATCCCTATCCCTGCGGATACCAGGGATAATGAAGTGAATCACGCCCTGGAACAGCAGGAAGCCA; AGAGGCGTGAAGCGGAGCAGGCTGCCAGTGAGGCGGCTGGTGGAGACACTACACCAGGGTCTTCCCCTTC; ATCTCTGTACTATGAAGAACCTCTGGGGCAACCTCCCCGGTTCACTCAAAAGTTACGGAGCAGAGAAGTT; CCAGAAGGAACTCGAGTACAGTTGGATTGCATAGTGGTAGGAATTCCACCACCTCAAGTAAGGTGGTACT; GTGAAGGCAAGGAGCTTGAAAATTCCCCAGATATTCACATCGTCCAGGCAGGAAATCTGCACTCACTGAC; CATTGCGGAAGCCTTTGAAGAGGACACAGGACGCTATTCCTGCTTTGCTTCTAACATCTATGGGACAGAT; TCGACTTCTGCTGAGATTTATATAGAAGGGGTTTCTTCTTCTGACTCAGAAGGCGACCCTAACAAGGAAG; AGATGAATCGAATCCAGAAGCCAAATGAGGTGTCATCTCCTCCCACTACCTCTGCAGTCATTCCTCCAGC; AGTACCCCAAGCCCAGCATTTGGTGGCCCAACCTCGTGTGGCAACCATCCAGCAGTGTCAGAGCCCCACC; AATTACTTGCAGGGATTGGATGGAAAACCTATCATTGCAGCTCCTGTGTTTACAAAGATGCTACAAAATT; TGTCAGCTTCTGAGGGTCAGCTGGTTGTCTTTGAATGCAGAGTAAAAGGAGCTCCATCTCCTAAGGTTGA; GTGGTATAGAGAAGGGACTTTAATAGAAGATTCTCCAGATTTTAGGATTTTACAGAAAAAACCTCGATCC; ATGGCAGAGCCAGAGGAGATTTGCACCTTGGTCATTGCTGAGGTGTTTGCAGAAGATTCTGGGTGCTTCA; CATGTACTGCAAGCAACAAATACGGCACAGTGTCAAGCATTGCACAGCTGCACGTGAGAGGAAATGAGGA; CCTCAGCAACAACGGGTCTCTTCACTCAGCCAACTCTACCACCAACCTGGCAGCTATTGAGCCACAGCCC; TCCCCACCCCACTCAGAGCCTCCATCTGTGGAACAACCCCCCAAACCCAAACTCGAGGGGGTTCTGGTGA; ACCACAATGAGCCCCGGTCCAGCTCCAGGATTGGGCTTCGTGTGCACTTCAACCTGCCTGAAGATGACAA; AGGAAGTGAAGCATCCTCCGAGGCTGGTGTGGTGACCACCAGACAGACCAGGCCCGATTCTTTCCAGGAG; AGGTTCAACGGACAGGCAACAAAAACCCCAGAGCCTTCTTCCCCCGTGAAAGAGCCCCCTCCAGTTCTGG; CCAAACCCAAACTTGATTCCACTCAGTTACAACAGCTTCATAACCAAGTCTTACTGGAACAACACCAATT; GCAAAACCCACCTCCTTCATCTCCTAAGGAGTTTCCTTTCAACATGACTGTTTTGAACTCCAATGCTCCC; CCAGCGGTGACAACATCCAATAAGCAGGTGAAGGCTCCTTCATCACAGACGTTCAGCTTGGCCCGGCCGA; AGTATTTCTTCCCCTCCACGAACACCACCGCAGCAACTGTGGCCCCTTCCAGCTCTCCGGTGTTCACTTT; GAGCAGCACTCCTCAAACTATTCAGAGGACAGTGAGCAAAGAAAGCCTCTTAGTGTCTCACCCCTCTGTG; CAAACCAAATCTCCAGGAGGGCTTTCCATCCAAAATGAGCCACTCCCACCAGGCCCAACAGAACCAACAC; CACCACCATTCACATTTTCCATCCCCAGGGGAAACCAGTTTCAGCCCCGCTGTGTGTCCCCAATTCCTGT; CTCTCCTACCAGCCGGATTCAGAACCCAGTGGCTTTCCTCAGCTCTGTTCTGCCTTCTCTCCCTGCCATC; CCACCCACAAATGCCATGGGGCTGCCTAGAAGTGCACCATCCATGCCATCCCAGGGATTAGCGAAGAAAA; ATACAAAGTCTCCTCAACCAGTGAATGATGATAACATTCGTGAAACTAAGAACGCAGTGATTCGAGACTT; GGGAAAAAAAATAACTTTCAGTGATGTCAGACCAAACCAGCAGGAGTACAAAATTTCAAGCTTTGAGCAG; AGGCTGATGAATGAAATAGAGTTTCGCTTGGAACGTACTCCTGTTGATGAATCAGATGATGAAATTCAAC; ATGATGAGATCCCCACGGGCAAGTGTATTGCTCCCATCTTTGACAAGAGACTCAAGCACTTCCGGGTCAC; AGAAGGCTCTCCAGTCACATTCACCTGCAAAATTGTTGGGATACCTGTTCCAAAGGTTTACTGGTTCAAA; GATGGGAAGCAGATTTCTAAGAGAAATGAGCACTGCAAAATGAGGCGAGAAGGAGATGGGACATGCTCTC; TGCACATTGAATCCACTACCAGTGATGACGATGGCAACTACACCATCATGGCAGCCAACCCCCAGGGGAG; AATCAGCTGTTCTGGCCACTTGATGGTACAAAGTTTGCCCATTCGCAGTCGGCTAACCTCTGCTGGTCAG; TCTCACAGGGGAAGATCCCGAGTGCAAGAAAGAGACAAAGAGCCCCTACAGGAACGCTTTTTCCGACCAC; ATTTCCTGCAGGCTCCTGGGGATATGGTAGCTCATGAGGGGCGCCTCTGTCGGCTGGACTGTAAGGTGAG; TGGTTTACCGCCCCCGGAGCTGACATGGCTACTCAATGGCCAACCTGTGCTACCAGATGCCTCCCACAAG; ATGCTGGTCAGGGAGACCGGAGTCCACTCTCTGCTCATTGACCCACTCACTCAGCGCGACGCAGGGACCT; ATAAGTGCATCGCTACCAACAAAACCGGGCAGAATTCTTTTAGTCTGGAGCTCTCTGTAGTAGCCAAAGA; GGTGAAGAAAGCACCTGTGATCCTGGAGAAACTACAGAACTGCGGTGTTCCCGAAGGCCACCCCGTGAGA; CTGGAGTGCCGCGTGATAGGCATGCCCCCACCTGTGTTCTACTGGAAGAAAGACAATGAGACCATCCCTT; GCACCAGAGAGAGGATCAGTATGCACCAGGACACAACAGGGTATGCCTGCCTTCTCATTCAGCCAGCCAA; GAAATCAGACGCTGGATGGTACACGTTGTCAGCCAAGAATGAAGCCGGCATCGTGTCGTGCACTGCCAGG; CTGGATATATACGCTCAGTGGCACCATCAGATCCCACCGCCCATGTCTGTCCGGCCCAGTGGCAGTCGCT; ACGGATCTCTCACCAGTAAAGGACTTGACATATTTTCTGCCTTTTCCTCCATGGAAAGCACGATGGTGTA; TTCATGCTCTTCTCGGAGTGTAGTGGAGAGTGATGAACTT; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC213189 representing NM_032578; Red=Cloning site Green=Tags(s); ; MQDDSIEASTSISQLLRESYLAETRHRGNNERSRAEPSSNPCHFGSPSGAAEGGGGQDDLPDLSAFLSQE; ELDESVNLARLAINYDPLEKADETQARKRLSPDQMKHSPNLSFEPNFCQDNPRSPTSSKESPQEAKRPQY; CSETQSKKVFLNKAADFIEELSSLFKSHSSKRIRPRACKNHKSKLESQNKVMQENSSSFSDLSERRERSS; VPIPIPADTRDNEVNHALEQQEAKRREAEQAASEAAGGDTTPGSSPSSLYYEEPLGQPPRFTQKLRSREV; PEGTRVQLDCIVVGIPPPQVRWYCEGKELENSPDIHIVQAGNLHSLTIAEAFEEDTGRYSCFASNIYGTD; STSAEIYIEGVSSSDSEGDPNKEEMNRIQKPNEVSSPPTTSAVIPPAVPQAQHLVAQPRVATIQQCQSPT; NYLQGLDGKPIIAAPVFTKMLQNLSASEGQLVVFECRVKGAPSPKVEWYREGTLIEDSPDFRILQKKPRS; MAEPEEICTLVIAEVFAEDSGCFTCTASNKYGTVSSIAQLHVRGNEDLSNNGSLHSANSTTNLAAIEPQP; SPPHSEPPSVEQPPKPKLEGVLVNHNEPRSSSRIGLRVHFNLPEDDKGSEASSEAGVVTTRQTRPDSFQE; RFNGQATKTPEPSSPVKEPPPVLAKPKLDSTQLQQLHNQVLLEQHQLQNPPPSSPKEFPFNMTVLNSNAP; PAVTTSNKQVKAPSSQTFSLARPKYFFPSTNTTAATVAPSSSPVFTLSSTPQTIQRTVSKESLLVSHPSV; QTKSPGGLSIQNEPLPPGPTEPTPPPFTFSIPRGNQFQPRCVSPIPVSPTSRIQNPVAFLSSVLPSLPAI; PPTNAMGLPRSAPSMPSQGLAKKNTKSPQPVNDDNIRETKNAVIRDLGKKITFSDVRPNQQEYKISSFEQ; RLMNEIEFRLERTPVDESDDEIQHDEIPTGKCIAPIFDKRLKHFRVTEGSPVTFTCKIVGIPVPKVYWFK; DGKQISKRNEHCKMRREGDGTCSLHIESTTSDDDGNYTIMAANPQGRISCSGHLMVQSLPIRSRLTSAGQ; SHRGRSRVQERDKEPLQERFFRPHFLQAPGDMVAHEGRLCRLDCKVSGLPPPELTWLLNGQPVLPDASHK; MLVRETGVHSLLIDPLTQRDAGTYKCIATNKTGQNSFSLELSVVAKEVKKAPVILEKLQNCGVPEGHPVR; LECRVIGMPPPVFYWKKDNETIPCTRERISMHQDTTGYACLLIQPAKKSDAGWYTLSAKNEAGIVSCTAR; LDIYAQWHHQIPPPMSVRPSGSRYGSLTSKGLDIFSAFSSMESTMVYSCSSRSVVESDEL; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Chromatograms: Chromatograms ; Sequencher program is needed, download here
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_032578
• ORF Size: 3960 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_032578.1, NP_115967.1
• RefSeq Size: 5707 bp
• RefSeq ORF: 3963 bp
• Locus ID: 84665
• UniProt ID: Q86TC9
• Cytogenetics: 10q21.3
• MW: 145.1 kDa
• Summary: Striated muscle in vertebrates comprises large proteins which must be organized properly to contract efficiently. Z-lines in striated muscle are a sign of this organization, representing the ends of actin thin filaments, titin, nebulin or nebulette and accessory proteins required for structure and function. This gene encodes a protein which interacts with nebulin in skeletal muscle or nebulette in cardiac muscle and alpha-actinin. In addition, this gene product can interact with a protein with the I-band indicating it has a regulatory as well as structural function. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2011]