IRS2 (NM_003749) Human Tagged ORF Clone

CAT#: RC212413

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IRS2 (Myc-DDK-tagged)-Human insulin receptor substrate 2 (IRS2)



  "NM_003749" in other vectors (6)

Special Offer: 20% off the control vector (empty cloning vector without insert). Use code "Shuttle20".

USD 1,587.00


Availability*
In Stock

Size
    • 10 ug


Product images

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol IRS2
Synonyms IRS-2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC212413 representing NM_003749.
Blue=ORF Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGGCGAGCCCGCCGCGGCACGGGCCGCCCGGGCCGGCGAGCGGAGACGGCCCCAACCTCAACAACAAC
AACAACAACAACAACCACAGCGTGCGCAAGTGCGGCTACCTGCGCAAGCAGAAGCATGGCCACAAGCGC
TTCTTCGTGCTGCGCGGACCCGGCGCGGGCGGCGACGAGGCGACGGCGGGCGGGGGGTCGGCGCCGCAA
CCGCCGCGGCTCGAGTACTACGAGAGCGAGAAAAAGTGGCGGAGCAAGGCAGGCGCGCCGAAACGGGTG
ATCGCTCTCGACTGCTGCCTGAACATCAACAAGCGCGCCGACGCCAAGCACAAGTACCTGATCGCCCTC
TACACCAAGGACGAGTACTTCGCCGTGGCCGCCGAGAACGAGCAGGAGCAGGAGGGCTGGTACCGCGCG
CTCACCGACCTGGTCAGCGAGGGCCGCGCGGCCGCCGGAGACGCGCCCCCCGCCGCCGCGCCCGCCGCG
TCCTGCAGCGCCTCCCTGCCCGGCGCCCTGGGCGGCTCTGCCGGCGCCGCCGGGGCCGAGGACAGCTAC
GGGCTGGTGGCTCCCGCCACGGCCGCCTACCGTGAGGTGTGGCAGGTGAACCTGAAGCCCAAGGGTCTG
GGCCAGAGCAAGAACCTGACGGGGGTGTACCGTCTGTGCCTGTCTGCGCGCACCATCGGCTTCGTGAAG
CTCAACTGCGAGCAGCCGTCGGTGACGCTGCAGCTCATGAACATCCGCCGCTGCGGCCACTCGGACAGC
TTCTTCTTCATCGAGGTGGGCCGCTCGGCCGTCACAGGCCCCGGCGAGCTGTGGATGCAGGCGGACGAC
TCGGTGGTGGCGCAGAACATCCACGAGACCATCCTGGAGGCCATGAAGGCGCTCAAGGAGCTCTTCGAG
TTCCGGCCGCGCAGTAAGAGCCAATCGTCGGGGTCGTCGGCCACGCACCCCATCAGCGTCCCCGGCGCG
CGCCGCCACCACCACCTGGTCAACCTGCCCCCCAGCCAGACGGGCCTGGTGCGCCGCTCGCGCACCGAC
AGCCTGGCCGCCACCCCGCCGGCGGCCAAGTGCAGCTCGTGCCGGGTGCGCACCGCCAGCGAGGGCGAC
GGCGGCGCGGCGGCGGGAGCGGCGGCCGCGGGCGCCAGGCCGGTGTCGGTGGCTGGGAGCCCCCTGAGC
CCCGGGCCGGTGCGCGCGCCCCTGAGCCGCTCGCACACCCTGAGCGGCGGCTGCGGCGGCCGCGGGAGC
AAGGTGGCGCTGCTGCCGGCAGGGGGCGCGCTGCAACACAGCCGCTCCATGTCCATGCCCGTGGCGCAC
TCGCCGCCCGCCGCCACCAGCCCCGGCTCCCTGTCGTCCAGCAGCGGCCACGGCTCGGGCTCCTACCCG
CCGCCGCCCGGCCCGCACCCGCCTCTGCCGCATCCGCTGCACCACGGCCCCGGCCAGCGGCCCTCCAGC
GGCAGCGCCTCCGCCTCGGGCTCCCCCAGCGACCCCGGCTTCATGTCCCTGGACGAGTACGGCTCCAGC
CCAGGCGACCTGCGCGCCTTCTGCAGCCACCGAAGCAACACGCCCGAGTCCATCGCGGAGACGCCCCCG
GCCCGAGACGGCGGCGGCGGCGGTGAGTTCTACGGGTACATGACCATGGACAGGCCCCTGAGCCACTGT
GGCCGCTCCTACCGCCGGGTCTCGGGGGACGCGGCCCAGGACCTGGACCGAGGGCTGCGCAAGAGGACC
TACTCCCTGACCACGCCAGCCCGGCAGCGGCCGGTGCCCCAGCCCTCCTCTGCCTCGCTGGATGAATAC
ACCCTGATGCGGGCCACCTTCTCGGGCAGCGCGGGCCGCCTCTGCCCGTCCTGCCCCGCGTCCTCTCCC
AAGGTGGCCTACCACCCCTACCCAGAGGACTACGGAGACATCGAGATCGGCTCCCACAGGAGCTCCAGC
AGCAACCTGGGGGCAGACGACGGCTACATGCCCATGACGCCCGGCGCGGCCCTCGCGGGCAGTGGGAGC
GGCAGCTGCAGGAGCGACGACTACATGCCCATGAGCCCCGCCAGCGTGTCCGCCCCCAAGCAGATCTTG
CAGCCCAGGGCCGCCGCCGCCGCCGCCGCCGCCGTGCCTTCTGCGGGGCCTGCGGGGCCAGCACCCACC
TCTGCGGCGGGCAGGACATTCCCGGCGAGCGGGGGCGGCTACAAGGCCAGCTCGCCCGCCGAGAGCTCC
CCCGAGGACAGTGGGTACATGCGCATGTGGTGCGGTTCCAAGCTGTCCATGGAGCATGCAGATGGCAAG
CTGCTGCCCAACGGGGACTACCTCAACGTGTCCCCCAGCGACGCGGTCACCACGGGCACCCCGCCCGAC
TTCTTCTCCGCAGCCCTGCACCCCGGCGGGGAGCCGCTCAGGGGCGTTCCCGGCTGCTGCTACAGCTCC
TTGCCCCGCTCCTACAAGGCCCCCTACACCTGTGGCGGGGACAGCGACCAGTACGTGCTCATGAGCTCC
CCTGTGGGGCGCATCCTGGAGGAGGAGCGTCTGGAGCCTCAGGCCACGCCAGGGCCCAGCCAGGCGGCC
AGCGCCTTCGGGGCCGGCCCCACGCAGCCCCCTCACCCTGTAGTGCCTTCGCCCGTGCGGCCTAGCGGC
GGCAGCCCGGAGGGCTTCTTGGGCCAGCGCGGCCGGGCGGTGAGGCCCACGCGCCTGTCCCTGGAGGGG
CTGCCCAGCCTGCCCAGCATGCACGAGTACCCACTGCCACCGGAGCCCAAGAGCCCCGGCGAGTACATC
AACATCGACTTTGGCGAGCCCGGGGCCCGCCTGTCGCCGCCCGCGCCTCCCCTGCTGGCGTCGGCGGCC
TCGTCCTCCTCGCTCTTGTCCGCCAGCAGCCCGGCCTCGTCGCTGGGCTCAGGCACCCCGGGCACCAGC
AGCGACAGCCGGCAGCGGTCTCCGCTCTCCGACTACATGAACCTCGACTTCAGCTCCCCCAAGTCTCCT
AAGCCGGGCGCCCCGAGCGGCCACCCCGTGGGCTCCTTGGACGGCCTCCTGTCCCCCGAGGCCTCCTCC
CCGTATCCGCCGTTGCCCCCGCGTCCGTCCGCGTCCCCGTCGTCGTCTCTGCAGCCGCCGCCACCGCCG
CCGGCCCCGGGGGAGCTGTACCGCCTGCCCCCCGCCTCGGCCGTTGCCACCGCCCAGGGCCCGGGCGCC
GCCTCATCGTTGTCCTCGGACACCGGGGACAATGGTGACTACACCGAGATGGCTTTTGGTGTGGCCGCC
ACCCCGCCGCAACCTATCGCGGCCCCCCCGAAGCCAGAAGCTGCCCGCGTGGCCAGCCCGACGTCGGGC
GTGAAGAGGCTGAGCCTCATGGAGCAGGTGTCGGGAGTCGAGGCCTTCCTGCAGGCCAGCCAGCCCCCG
GACCCCCACCGCGGCGCCAAGGTCATCCGCGCAGACCCGCAGGGGGGCCGCCGCCGCCACAGTTCCGAG
ACCTTCTCCTCCACCACGACGGTCACCCCCGTGTCCCCGTCCTTCGCCCACAACCCCAAGCGCCACAAC
TCGGCCTCCGTGGAAAATGTCTCTCTCAGGAAAAGCAGCGAGGGCGGCGTGGGTGTCGGCCCTGGAGGG
GGCGACGAGCCGCCCACCTCCCCACGACAGTTGCAGCCGGCGCCCCCTTTGGCACCGCAGGGCCGGCCG
TGGACCCCGGGTCAGCCCGGGGGCTTGGTCGGTTGTCCTGGGAGCGGTGGATCGCCCATGCGCAGAGAG
ACCTCTGCCGGCTTCCAGAATGGTCTCAACTACATCGCCATCGACGTGAGGGAGGAGCCCGGGCTGCCA
CCCCAGCCGCAGCCGCCGCCGCCGCCGCTTCCTCAGCCGGGAGACAAGAGCTCCTGGGGCCGGACCCGA
AGCCTCGGGGGTCTCATCAGCGCTGTGGGCGTCGGCAGCACCGGCGGCGGGTGCGGGGGGCCGGGTCCC
GGTGCCCTGCCCCCTGCCAACACCTACGCCAGCATTGACTTCTTGTCCCACCACTTGAAGGAGGCCACC
ATCGTGAAAGAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAG
GTTTAAACGGCCGGC
>Peptide sequence encoded by RC212413
Blue=ORF Red=Cloning site Green=Tag(s)

MASPPRHGPPGPASGDGPNLNNNNNNNNHSVRKCGYLRKQKHGHKRFFVLRGPGAGGDEATAGGGSAPQ
PPRLEYYESEKKWRSKAGAPKRVIALDCCLNINKRADAKHKYLIALYTKDEYFAVAAENEQEQEGWYRA
LTDLVSEGRAAAGDAPPAAAPAASCSASLPGALGGSAGAAGAEDSYGLVAPATAAYREVWQVNLKPKGL
GQSKNLTGVYRLCLSARTIGFVKLNCEQPSVTLQLMNIRRCGHSDSFFFIEVGRSAVTGPGELWMQADD
SVVAQNIHETILEAMKALKELFEFRPRSKSQSSGSSATHPISVPGARRHHHLVNLPPSQTGLVRRSRTD
SLAATPPAAKCSSCRVRTASEGDGGAAAGAAAAGARPVSVAGSPLSPGPVRAPLSRSHTLSGGCGGRGS
KVALLPAGGALQHSRSMSMPVAHSPPAATSPGSLSSSSGHGSGSYPPPPGPHPPLPHPLHHGPGQRPSS
GSASASGSPSDPGFMSLDEYGSSPGDLRAFCSHRSNTPESIAETPPARDGGGGGEFYGYMTMDRPLSHC
GRSYRRVSGDAAQDLDRGLRKRTYSLTTPARQRPVPQPSSASLDEYTLMRATFSGSAGRLCPSCPASSP
KVAYHPYPEDYGDIEIGSHRSSSSNLGADDGYMPMTPGAALAGSGSGSCRSDDYMPMSPASVSAPKQIL
QPRAAAAAAAAVPSAGPAGPAPTSAAGRTFPASGGGYKASSPAESSPEDSGYMRMWCGSKLSMEHADGK
LLPNGDYLNVSPSDAVTTGTPPDFFSAALHPGGEPLRGVPGCCYSSLPRSYKAPYTCGGDSDQYVLMSS
PVGRILEEERLEPQATPGPSQAASAFGAGPTQPPHPVVPSPVRPSGGSPEGFLGQRGRAVRPTRLSLEG
LPSLPSMHEYPLPPEPKSPGEYINIDFGEPGARLSPPAPPLLASAASSSSLLSASSPASSLGSGTPGTS
SDSRQRSPLSDYMNLDFSSPKSPKPGAPSGHPVGSLDGLLSPEASSPYPPLPPRPSASPSSSLQPPPPP
PAPGELYRLPPASAVATAQGPGAASSLSSDTGDNGDYTEMAFGVAATPPQPIAAPPKPEAARVASPTSG
VKRLSLMEQVSGVEAFLQASQPPDPHRGAKVIRADPQGGRRRHSSETFSSTTTVTPVSPSFAHNPKRHN
SASVENVSLRKSSEGGVGVGPGGGDEPPTSPRQLQPAPPLAPQGRPWTPGQPGGLVGCPGSGGSPMRRE
TSAGFQNGLNYIAIDVREEPGLPPQPQPPPPPLPQPGDKSSWGRTRSLGGLISAVGVGSTGGGCGGPGP
GALPPANTYASIDFLSHHLKEATIVKE

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_003749
ORF Size 4014 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq Size 7014 bp
RefSeq ORF 4017 bp
Locus ID 8660
UniProt ID Q9Y4H2
Cytogenetics 13q34
Protein Families Druggable Genome
Protein Pathways Adipocytokine signaling pathway, Insulin signaling pathway, Neurotrophin signaling pathway, Type II diabetes mellitus
MW 137.3 kDa
Gene Summary This gene encodes the insulin receptor substrate 2, a cytoplasmic signaling molecule that mediates effects of insulin, insulin-like growth factor 1, and other cytokines by acting as a molecular adaptor between diverse receptor tyrosine kinases and downstream effectors. The product of this gene is phosphorylated by the insulin receptor tyrosine kinase upon receptor stimulation, as well as by an interleukin 4 receptor-associated kinase in response to IL4 treatment. [provided by RefSeq, Jul 2008]

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