IRS2 (NM_003749) Human Tagged ORF Clone

CAT#: RC212413

IRS2 (Myc-DDK-tagged)-Human insulin receptor substrate 2 (IRS2)

Product Images

Circular map for RC212413

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY IRS2 (Cat# RC212413, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IRS2 antibody(Cat# TA890130). Positive lysates LY418459 (100ug) and LC418459 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY418459) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC212413 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: IRS2
• Synonyms: IRS-2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC212413 representing NM_003749.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGCGAGCCCGCCGCGGCACGGGCCGCCCGGGCCGGCGAGCGGAGACGGCCCCAACCTCAACAACAAC
  AACAACAACAACAACCACAGCGTGCGCAAGTGCGGCTACCTGCGCAAGCAGAAGCATGGCCACAAGCGC
  TTCTTCGTGCTGCGCGGACCCGGCGCGGGCGGCGACGAGGCGACGGCGGGCGGGGGGTCGGCGCCGCAA
  CCGCCGCGGCTCGAGTACTACGAGAGCGAGAAAAAGTGGCGGAGCAAGGCAGGCGCGCCGAAACGGGTG
  ATCGCTCTCGACTGCTGCCTGAACATCAACAAGCGCGCCGACGCCAAGCACAAGTACCTGATCGCCCTC
  TACACCAAGGACGAGTACTTCGCCGTGGCCGCCGAGAACGAGCAGGAGCAGGAGGGCTGGTACCGCGCG
  CTCACCGACCTGGTCAGCGAGGGCCGCGCGGCCGCCGGAGACGCGCCCCCCGCCGCCGCGCCCGCCGCG
  TCCTGCAGCGCCTCCCTGCCCGGCGCCCTGGGCGGCTCTGCCGGCGCCGCCGGGGCCGAGGACAGCTAC
  GGGCTGGTGGCTCCCGCCACGGCCGCCTACCGTGAGGTGTGGCAGGTGAACCTGAAGCCCAAGGGTCTG
  GGCCAGAGCAAGAACCTGACGGGGGTGTACCGTCTGTGCCTGTCTGCGCGCACCATCGGCTTCGTGAAG
  CTCAACTGCGAGCAGCCGTCGGTGACGCTGCAGCTCATGAACATCCGCCGCTGCGGCCACTCGGACAGC
  TTCTTCTTCATCGAGGTGGGCCGCTCGGCCGTCACAGGCCCCGGCGAGCTGTGGATGCAGGCGGACGAC
  TCGGTGGTGGCGCAGAACATCCACGAGACCATCCTGGAGGCCATGAAGGCGCTCAAGGAGCTCTTCGAG
  TTCCGGCCGCGCAGTAAGAGCCAATCGTCGGGGTCGTCGGCCACGCACCCCATCAGCGTCCCCGGCGCG
  CGCCGCCACCACCACCTGGTCAACCTGCCCCCCAGCCAGACGGGCCTGGTGCGCCGCTCGCGCACCGAC
  AGCCTGGCCGCCACCCCGCCGGCGGCCAAGTGCAGCTCGTGCCGGGTGCGCACCGCCAGCGAGGGCGAC
  GGCGGCGCGGCGGCGGGAGCGGCGGCCGCGGGCGCCAGGCCGGTGTCGGTGGCTGGGAGCCCCCTGAGC
  CCCGGGCCGGTGCGCGCGCCCCTGAGCCGCTCGCACACCCTGAGCGGCGGCTGCGGCGGCCGCGGGAGC
  AAGGTGGCGCTGCTGCCGGCAGGGGGCGCGCTGCAACACAGCCGCTCCATGTCCATGCCCGTGGCGCAC
  TCGCCGCCCGCCGCCACCAGCCCCGGCTCCCTGTCGTCCAGCAGCGGCCACGGCTCGGGCTCCTACCCG
  CCGCCGCCCGGCCCGCACCCGCCTCTGCCGCATCCGCTGCACCACGGCCCCGGCCAGCGGCCCTCCAGC
  GGCAGCGCCTCCGCCTCGGGCTCCCCCAGCGACCCCGGCTTCATGTCCCTGGACGAGTACGGCTCCAGC
  CCAGGCGACCTGCGCGCCTTCTGCAGCCACCGAAGCAACACGCCCGAGTCCATCGCGGAGACGCCCCCG
  GCCCGAGACGGCGGCGGCGGCGGTGAGTTCTACGGGTACATGACCATGGACAGGCCCCTGAGCCACTGT
  GGCCGCTCCTACCGCCGGGTCTCGGGGGACGCGGCCCAGGACCTGGACCGAGGGCTGCGCAAGAGGACC
  TACTCCCTGACCACGCCAGCCCGGCAGCGGCCGGTGCCCCAGCCCTCCTCTGCCTCGCTGGATGAATAC
  ACCCTGATGCGGGCCACCTTCTCGGGCAGCGCGGGCCGCCTCTGCCCGTCCTGCCCCGCGTCCTCTCCC
  AAGGTGGCCTACCACCCCTACCCAGAGGACTACGGAGACATCGAGATCGGCTCCCACAGGAGCTCCAGC
  AGCAACCTGGGGGCAGACGACGGCTACATGCCCATGACGCCCGGCGCGGCCCTCGCGGGCAGTGGGAGC
  GGCAGCTGCAGGAGCGACGACTACATGCCCATGAGCCCCGCCAGCGTGTCCGCCCCCAAGCAGATCTTG
  CAGCCCAGGGCCGCCGCCGCCGCCGCCGCCGCCGTGCCTTCTGCGGGGCCTGCGGGGCCAGCACCCACC
  TCTGCGGCGGGCAGGACATTCCCGGCGAGCGGGGGCGGCTACAAGGCCAGCTCGCCCGCCGAGAGCTCC
  CCCGAGGACAGTGGGTACATGCGCATGTGGTGCGGTTCCAAGCTGTCCATGGAGCATGCAGATGGCAAG
  CTGCTGCCCAACGGGGACTACCTCAACGTGTCCCCCAGCGACGCGGTCACCACGGGCACCCCGCCCGAC
  TTCTTCTCCGCAGCCCTGCACCCCGGCGGGGAGCCGCTCAGGGGCGTTCCCGGCTGCTGCTACAGCTCC
  TTGCCCCGCTCCTACAAGGCCCCCTACACCTGTGGCGGGGACAGCGACCAGTACGTGCTCATGAGCTCC
  CCTGTGGGGCGCATCCTGGAGGAGGAGCGTCTGGAGCCTCAGGCCACGCCAGGGCCCAGCCAGGCGGCC
  AGCGCCTTCGGGGCCGGCCCCACGCAGCCCCCTCACCCTGTAGTGCCTTCGCCCGTGCGGCCTAGCGGC
  GGCAGCCCGGAGGGCTTCTTGGGCCAGCGCGGCCGGGCGGTGAGGCCCACGCGCCTGTCCCTGGAGGGG
  CTGCCCAGCCTGCCCAGCATGCACGAGTACCCACTGCCACCGGAGCCCAAGAGCCCCGGCGAGTACATC
  AACATCGACTTTGGCGAGCCCGGGGCCCGCCTGTCGCCGCCCGCGCCTCCCCTGCTGGCGTCGGCGGCC
  TCGTCCTCCTCGCTCTTGTCCGCCAGCAGCCCGGCCTCGTCGCTGGGCTCAGGCACCCCGGGCACCAGC
  AGCGACAGCCGGCAGCGGTCTCCGCTCTCCGACTACATGAACCTCGACTTCAGCTCCCCCAAGTCTCCT
  AAGCCGGGCGCCCCGAGCGGCCACCCCGTGGGCTCCTTGGACGGCCTCCTGTCCCCCGAGGCCTCCTCC
  CCGTATCCGCCGTTGCCCCCGCGTCCGTCCGCGTCCCCGTCGTCGTCTCTGCAGCCGCCGCCACCGCCG
  CCGGCCCCGGGGGAGCTGTACCGCCTGCCCCCCGCCTCGGCCGTTGCCACCGCCCAGGGCCCGGGCGCC
  GCCTCATCGTTGTCCTCGGACACCGGGGACAATGGTGACTACACCGAGATGGCTTTTGGTGTGGCCGCC
  ACCCCGCCGCAACCTATCGCGGCCCCCCCGAAGCCAGAAGCTGCCCGCGTGGCCAGCCCGACGTCGGGC
  GTGAAGAGGCTGAGCCTCATGGAGCAGGTGTCGGGAGTCGAGGCCTTCCTGCAGGCCAGCCAGCCCCCG
  GACCCCCACCGCGGCGCCAAGGTCATCCGCGCAGACCCGCAGGGGGGCCGCCGCCGCCACAGTTCCGAG
  ACCTTCTCCTCCACCACGACGGTCACCCCCGTGTCCCCGTCCTTCGCCCACAACCCCAAGCGCCACAAC
  TCGGCCTCCGTGGAAAATGTCTCTCTCAGGAAAAGCAGCGAGGGCGGCGTGGGTGTCGGCCCTGGAGGG
  GGCGACGAGCCGCCCACCTCCCCACGACAGTTGCAGCCGGCGCCCCCTTTGGCACCGCAGGGCCGGCCG
  TGGACCCCGGGTCAGCCCGGGGGCTTGGTCGGTTGTCCTGGGAGCGGTGGATCGCCCATGCGCAGAGAG
  ACCTCTGCCGGCTTCCAGAATGGTCTCAACTACATCGCCATCGACGTGAGGGAGGAGCCCGGGCTGCCA
  CCCCAGCCGCAGCCGCCGCCGCCGCCGCTTCCTCAGCCGGGAGACAAGAGCTCCTGGGGCCGGACCCGA
  AGCCTCGGGGGTCTCATCAGCGCTGTGGGCGTCGGCAGCACCGGCGGCGGGTGCGGGGGGCCGGGTCCC
  GGTGCCCTGCCCCCTGCCAACACCTACGCCAGCATTGACTTCTTGTCCCACCACTTGAAGGAGGCCACC
  ATCGTGAAAGAG
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
  Protein Sequence

  >Peptide sequence encoded by RC212413
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MASPPRHGPPGPASGDGPNLNNNNNNNNHSVRKCGYLRKQKHGHKRFFVLRGPGAGGDEATAGGGSAPQ
  PPRLEYYESEKKWRSKAGAPKRVIALDCCLNINKRADAKHKYLIALYTKDEYFAVAAENEQEQEGWYRA
  LTDLVSEGRAAAGDAPPAAAPAASCSASLPGALGGSAGAAGAEDSYGLVAPATAAYREVWQVNLKPKGL
  GQSKNLTGVYRLCLSARTIGFVKLNCEQPSVTLQLMNIRRCGHSDSFFFIEVGRSAVTGPGELWMQADD
  SVVAQNIHETILEAMKALKELFEFRPRSKSQSSGSSATHPISVPGARRHHHLVNLPPSQTGLVRRSRTD
  SLAATPPAAKCSSCRVRTASEGDGGAAAGAAAAGARPVSVAGSPLSPGPVRAPLSRSHTLSGGCGGRGS
  KVALLPAGGALQHSRSMSMPVAHSPPAATSPGSLSSSSGHGSGSYPPPPGPHPPLPHPLHHGPGQRPSS
  GSASASGSPSDPGFMSLDEYGSSPGDLRAFCSHRSNTPESIAETPPARDGGGGGEFYGYMTMDRPLSHC
  GRSYRRVSGDAAQDLDRGLRKRTYSLTTPARQRPVPQPSSASLDEYTLMRATFSGSAGRLCPSCPASSP
  KVAYHPYPEDYGDIEIGSHRSSSSNLGADDGYMPMTPGAALAGSGSGSCRSDDYMPMSPASVSAPKQIL
  QPRAAAAAAAAVPSAGPAGPAPTSAAGRTFPASGGGYKASSPAESSPEDSGYMRMWCGSKLSMEHADGK
  LLPNGDYLNVSPSDAVTTGTPPDFFSAALHPGGEPLRGVPGCCYSSLPRSYKAPYTCGGDSDQYVLMSS
  PVGRILEEERLEPQATPGPSQAASAFGAGPTQPPHPVVPSPVRPSGGSPEGFLGQRGRAVRPTRLSLEG
  LPSLPSMHEYPLPPEPKSPGEYINIDFGEPGARLSPPAPPLLASAASSSSLLSASSPASSLGSGTPGTS
  SDSRQRSPLSDYMNLDFSSPKSPKPGAPSGHPVGSLDGLLSPEASSPYPPLPPRPSASPSSSLQPPPPP
  PAPGELYRLPPASAVATAQGPGAASSLSSDTGDNGDYTEMAFGVAATPPQPIAAPPKPEAARVASPTSG
  VKRLSLMEQVSGVEAFLQASQPPDPHRGAKVIRADPQGGRRRHSSETFSSTTTVTPVSPSFAHNPKRHN
  SASVENVSLRKSSEGGVGVGPGGGDEPPTSPRQLQPAPPLAPQGRPWTPGQPGGLVGCPGSGGSPMRRE
  TSAGFQNGLNYIAIDVREEPGLPPQPQPPPPPLPQPGDKSSWGRTRSLGGLISAVGVGSTGGGCGGPGP
  GALPPANTYASIDFLSHHLKEATIVKE
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003749
• ORF Size: 4014 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.

Reference Data

• RefSeq Size: 7014 bp
• RefSeq ORF: 4017 bp
• Locus ID: 8660
• UniProt ID: Q9Y4H2
• Cytogenetics: 13q34
• Protein Families: Druggable Genome
• Protein Pathways: Adipocytokine signaling pathway, Insulin signaling pathway, Neurotrophin signaling pathway, Type II diabetes mellitus
• MW: 137.3 kDa
• Gene Summary: This gene encodes the insulin receptor substrate 2, a cytoplasmic signaling molecule that mediates effects of insulin, insulin-like growth factor 1, and other cytokines by acting as a molecular adaptor between diverse receptor tyrosine kinases and downstream effectors. The product of this gene is phosphorylated by the insulin receptor tyrosine kinase upon receptor stimulation, as well as by an interleukin 4 receptor-associated kinase in response to IL4 treatment. [provided by RefSeq, Jul 2008]