IRS2 (NM_003749) Human Tagged ORF Clone

CAT#: RG212413

IRS2 (tGFP-tagged) - Human insulin receptor substrate 2 (IRS2)

Product Images

Circular map for RG212413

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: IRS2
• Synonyms: IRS-2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG212413 representing NM_003749
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGAGCCCGCCGCGGCACGGGCCGCCCGGGCCGGCGAGCGGAGACGGCCCCAACCTCAACAACAACA
  ACAACAACAACAACCACAGCGTGCGCAAGTGCGGCTACCTGCGCAAGCAGAAGCATGGCCACAAGCGCTT
  CTTCGTGCTGCGCGGACCCGGCGCGGGCGGCGACGAGGCGACGGCGGGCGGGGGGTCGGCGCCGCAACCG
  CCGCGGCTCGAGTACTACGAGAGCGAGAAAAAGTGGCGGAGCAAGGCAGGCGCGCCGAAACGGGTGATCG
  CTCTCGACTGCTGCCTGAACATCAACAAGCGCGCCGACGCCAAGCACAAGTACCTGATCGCCCTCTACAC
  CAAGGACGAGTACTTCGCCGTGGCCGCCGAGAACGAGCAGGAGCAGGAGGGCTGGTACCGCGCGCTCACC
  GACCTGGTCAGCGAGGGCCGCGCGGCCGCCGGAGACGCGCCCCCCGCCGCCGCGCCCGCCGCGTCCTGCA
  GCGCCTCCCTGCCCGGCGCCCTGGGCGGCTCTGCCGGCGCCGCCGGGGCCGAGGACAGCTACGGGCTGGT
  GGCTCCCGCCACGGCCGCCTACCGTGAGGTGTGGCAGGTGAACCTGAAGCCCAAGGGTCTGGGCCAGAGC
  AAGAACCTGACGGGGGTGTACCGTCTGTGCCTGTCTGCGCGCACCATCGGCTTCGTGAAGCTCAACTGCG
  AGCAGCCGTCGGTGACGCTGCAGCTCATGAACATCCGCCGCTGCGGCCACTCGGACAGCTTCTTCTTCAT
  CGAGGTGGGCCGCTCGGCCGTCACAGGCCCCGGCGAGCTGTGGATGCAGGCGGACGACTCGGTGGTGGCG
  CAGAACATCCACGAGACCATCCTGGAGGCCATGAAGGCGCTCAAGGAGCTCTTCGAGTTCCGGCCGCGCA
  GTAAGAGCCAATCGTCGGGGTCGTCGGCCACGCACCCCATCAGCGTCCCCGGCGCGCGCCGCCACCACCA
  CCTGGTCAACCTGCCCCCCAGCCAGACGGGCCTGGTGCGCCGCTCGCGCACCGACAGCCTGGCCGCCACC
  CCGCCGGCGGCCAAGTGCAGCTCGTGCCGGGTGCGCACCGCCAGCGAGGGCGACGGCGGCGCGGCGGCGG
  GAGCGGCGGCCGCGGGCGCCAGGCCGGTGTCGGTGGCTGGGAGCCCCCTGAGCCCCGGGCCGGTGCGCGC
  GCCCCTGAGCCGCTCGCACACCCTGAGCGGCGGCTGCGGCGGCCGCGGGAGCAAGGTGGCGCTGCTGCCG
  GCAGGGGGCGCGCTGCAACACAGCCGCTCCATGTCCATGCCCGTGGCGCACTCGCCGCCCGCCGCCACCA
  GCCCCGGCTCCCTGTCGTCCAGCAGCGGCCACGGCTCGGGCTCCTACCCGCCGCCGCCCGGCCCGCACCC
  GCCTCTGCCGCATCCGCTGCACCACGGCCCCGGCCAGCGGCCCTCCAGCGGCAGCGCCTCCGCCTCGGGC
  TCCCCCAGCGACCCCGGCTTCATGTCCCTGGACGAGTACGGCTCCAGCCCAGGCGACCTGCGCGCCTTCT
  GCAGCCACCGAAGCAACACGCCCGAGTCCATCGCGGAGACGCCCCCGGCCCGAGACGGCGGCGGCGGCGG
  TGAGTTCTACGGGTACATGACCATGGACAGGCCCCTGAGCCACTGTGGCCGCTCCTACCGCCGGGTCTCG
  GGGGACGCGGCCCAGGACCTGGACCGAGGGCTGCGCAAGAGGACCTACTCCCTGACCACGCCAGCCCGGC
  AGCGGCCGGTGCCCCAGCCCTCCTCTGCCTCGCTGGATGAATACACCCTGATGCGGGCCACCTTCTCGGG
  CAGCGCGGGCCGCCTCTGCCCGTCCTGCCCCGCGTCCTCTCCCAAGGTGGCCTACCACCCCTACCCAGAG
  GACTACGGAGACATCGAGATCGGCTCCCACAGGAGCTCCAGCAGCAACCTGGGGGCAGACGACGGCTACA
  TGCCCATGACGCCCGGCGCGGCCCTCGCGGGCAGTGGGAGCGGCAGCTGCAGGAGCGACGACTACATGCC
  CATGAGCCCCGCCAGCGTGTCCGCCCCCAAGCAGATCTTGCAGCCCAGGGCCGCCGCCGCCGCCGCCGCC
  GCCGTGCCTTCTGCGGGGCCTGCGGGGCCAGCACCCACCTCTGCGGCGGGCAGGACATTCCCGGCGAGCG
  GGGGCGGCTACAAGGCCAGCTCGCCCGCCGAGAGCTCCCCCGAGGACAGTGGGTACATGCGCATGTGGTG
  CGGTTCCAAGCTGTCCATGGAGCATGCAGATGGCAAGCTGCTGCCCAACGGGGACTACCTCAACGTGTCC
  CCCAGCGACGCGGTCACCACGGGCACCCCGCCCGACTTCTTCTCCGCAGCCCTGCACCCCGGCGGGGAGC
  CGCTCAGGGGCGTTCCCGGCTGCTGCTACAGCTCCTTGCCCCGCTCCTACAAGGCCCCCTACACCTGTGG
  CGGGGACAGCGACCAGTACGTGCTCATGAGCTCCCCCGTGGGGCGCATCCTGGAGGAGGAGCGTCTGGAG
  CCTCAGGCCACGCCAGGGCCCAGCCAGGCGGCCAGCGCCTTCGGGGCCGGCCCCACGCAGCCCCCTCACC
  CTGTAGTGCCTTCGCCCGTGCGGCCTAGCGGCGGCCGCCCGGAGGGCTTCTTGGGCCAGCGCGGCCGGGC
  GGTGAGGCCCACGCGCCTGTCCCTGGAGGGGCTGCCCAGCCTGCCCAGCATGCACGAGTACCCACTGCCA
  CCGGAGCCCAAGAGCCCCGGCGAGTACATCAACATCGACTTTGGCGAGCCCGGGGCCCGCCTGTCGCCGC
  CCGCGCCTCCCCTGCTGGCGTCGGCGGCCTCGTCCTCCTCGCTCTTGTCCGCCAGCAGCCCGGCCTCGTC
  GCTGGGCTCAGGCACCCCGGGCACCAGCAGCGACAGCCGGCAGCGGTCTCCGCTCTCCGACTACATGAAC
  CTCGACTTCAGCTCCCCCAAGTCTCCTAAGCCGGGCGCCCCGAGCGGCCACCCCGTGGGCTCCTTGGACG
  GCCTCCTGTCCCCCGAGGCCTCCTCCCCGTATCCGCCGTTGCCCCCGCGTCCGTCCGCGTCCCCGTCGTC
  GTCTCTGCAGCCGCCGCCACCGCCGCCGGCCCCGGGGGAGCTGTACCGCCTGCCCCCCGCCTCGGCCGTT
  GCCACCGCCCAGGGCCCGGGCGCCGCCTCATCGTTGTCCTCGGACACCGGGGACAATGGTGACTACACCG
  AGATGGCTTTTGGTGTGGCCGCCACCCCGCCGCAACCTATCGCGGCCCCCCCGAAGCCAGAAGCTGCCCG
  CGTGGCCAGCCCGACGTCGGGCGTGAAGAGGCTGAGCCTCATGGAGCAGGTGTCGGGAGTCGAGGCCTTC
  CTGCAGGCCAGCCAGCCCCCGGACCCCCACCGCGGCGCCAAGGTCATCCGCGCAGACCCGCAGGGGGGCC
  GCCGCCGCCACAGTTCCGAGACCTTCTCCTCCACCACGACGGTCACCCCCGTGTCCCCGTCCTTCGCCCA
  CAACCCCAAGCGCCACAACTCGGCCTCCGTGGAAAATGTCTCTCTCAGGAAAAGCAGCGAGGGCGGCGTG
  GGTGTCGGCCCTGGAGGGGGCGACGAGCCGCCCACCTCCCCACGACAGTTGCAGCCGGCGCCCCCTTTGG
  CACCGCAGGGCCGGCCGTGGACCCCGGGTCAGCCCGGGGGCTTGGTCGGTTGTCCTGGGAGCGGTGGATC
  GCCCATGCGCAGAGAGACCTCTGCCGGCTTCCAGAATGGTCTCAACTACATCGCCATCGACGTGAGGGAG
  GAGCCCGGGCTGCCACCCCAGCCGCAGCCGCCGCCGCCGCCGCTTCCTCAGCCGGGAGACAAGAGCTCCT
  GGGGCCGGACCCGAAGCCTCGGGGGTCTCATCAGCGCTGTGGGCGTCGGCAGCACCGGCGGCGGGTGCGG
  GGGGCCGGGTCCCGGTGCCCTGCCCCCTGCCAACACCTACGCCAGCATTGACTTCTTGTCCCACCACTTG
  AAGGAGGCCACCATCGTGAAAGAG
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG212413 representing NM_003749
  Red=Cloning site Green=Tags(s)
  
  MASPPRHGPPGPASGDGPNLNNNNNNNNHSVRKCGYLRKQKHGHKRFFVLRGPGAGGDEATAGGGSAPQP
  PRLEYYESEKKWRSKAGAPKRVIALDCCLNINKRADAKHKYLIALYTKDEYFAVAAENEQEQEGWYRALT
  DLVSEGRAAAGDAPPAAAPAASCSASLPGALGGSAGAAGAEDSYGLVAPATAAYREVWQVNLKPKGLGQS
  KNLTGVYRLCLSARTIGFVKLNCEQPSVTLQLMNIRRCGHSDSFFFIEVGRSAVTGPGELWMQADDSVVA
  QNIHETILEAMKALKELFEFRPRSKSQSSGSSATHPISVPGARRHHHLVNLPPSQTGLVRRSRTDSLAAT
  PPAAKCSSCRVRTASEGDGGAAAGAAAAGARPVSVAGSPLSPGPVRAPLSRSHTLSGGCGGRGSKVALLP
  AGGALQHSRSMSMPVAHSPPAATSPGSLSSSSGHGSGSYPPPPGPHPPLPHPLHHGPGQRPSSGSASASG
  SPSDPGFMSLDEYGSSPGDLRAFCSHRSNTPESIAETPPARDGGGGGEFYGYMTMDRPLSHCGRSYRRVS
  GDAAQDLDRGLRKRTYSLTTPARQRPVPQPSSASLDEYTLMRATFSGSAGRLCPSCPASSPKVAYHPYPE
  DYGDIEIGSHRSSSSNLGADDGYMPMTPGAALAGSGSGSCRSDDYMPMSPASVSAPKQILQPRAAAAAAA
  AVPSAGPAGPAPTSAAGRTFPASGGGYKASSPAESSPEDSGYMRMWCGSKLSMEHADGKLLPNGDYLNVS
  PSDAVTTGTPPDFFSAALHPGGEPLRGVPGCCYSSLPRSYKAPYTCGGDSDQYVLMSSPVGRILEEERLE
  PQATPGPSQAASAFGAGPTQPPHPVVPSPVRPSGGRPEGFLGQRGRAVRPTRLSLEGLPSLPSMHEYPLP
  PEPKSPGEYINIDFGEPGARLSPPAPPLLASAASSSSLLSASSPASSLGSGTPGTSSDSRQRSPLSDYMN
  LDFSSPKSPKPGAPSGHPVGSLDGLLSPEASSPYPPLPPRPSASPSSSLQPPPPPPAPGELYRLPPASAV
  ATAQGPGAASSLSSDTGDNGDYTEMAFGVAATPPQPIAAPPKPEAARVASPTSGVKRLSLMEQVSGVEAF
  LQASQPPDPHRGAKVIRADPQGGRRRHSSETFSSTTTVTPVSPSFAHNPKRHNSASVENVSLRKSSEGGV
  GVGPGGGDEPPTSPRQLQPAPPLAPQGRPWTPGQPGGLVGCPGSGGSPMRRETSAGFQNGLNYIAIDVRE
  EPGLPPQPQPPPPPLPQPGDKSSWGRTRSLGGLISAVGVGSTGGGCGGPGPGALPPANTYASIDFLSHHL
  KEATIVKE
  
  SGPTRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003749
• ORF Size: 4014 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003749.3
• RefSeq Size: 7014 bp
• RefSeq ORF: 4017 bp
• Locus ID: 8660
• UniProt ID: Q9Y4H2
• Cytogenetics: 13q34
• Protein Families: Druggable Genome
• Protein Pathways: Adipocytokine signaling pathway, Insulin signaling pathway, Neurotrophin signaling pathway, Type II diabetes mellitus
• Gene Summary: This gene encodes the insulin receptor substrate 2, a cytoplasmic signaling molecule that mediates effects of insulin, insulin-like growth factor 1, and other cytokines by acting as a molecular adaptor between diverse receptor tyrosine kinases and downstream effectors. The product of this gene is phosphorylated by the insulin receptor tyrosine kinase upon receptor stimulation, as well as by an interleukin 4 receptor-associated kinase in response to IL4 treatment. [provided by RefSeq, Jul 2008]