IRS2 (NM_003749) Human Untagged Clone

CAT#: SC303374

IRS2 (untagged)-Human insulin receptor substrate 2 (IRS2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: IRS2
• Synonyms: IRS-2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_003749 edited
  GCCTGAAGCGCCCGCGATGGCGAGCCCGCCGCGGCACGGGCCGCCCGGGCCGGCGAGCGG
  AGACGGCCCCAACCTCAACAACAACAACAACAACAACAACCACAGCGTGCGCAAGTGCGG
  CTACCTGCGCAAGCAGAAGCATGGCCACAAGCGCTTCTTCGTGCTGCGCGGACCCGGCGC
  GGGCGGCGACGAGGCGACGGCGGGCGGGGGGTCGGCGCCGCAACCGCCGCGGCTCGAGTA
  CTACGAGAGCGAGAAAAAGTGGCGGAGCAAGGCAGGCGCGCCGAAACGGGTGATCGCTCT
  CGACTGCTGCCTGAACATCAACAAGCGCGCCGACGCCAAGCACAAGTACCTGATCGCCCT
  CTACACCAAGGACGAGTACTTCGCCGTGGCCGCCGAGAACGAGCAGGAGCAGGAGGGCTG
  GTACCGCGCGCTCACCGACCTGGTCAGCGAGGGCCGCGCGGCCGCCGGAGACGCGCCCCC
  CGCCGCCGCGCCCGCCGCGTCCTGCAGCGCCTCCCTGCCCGGCGCCCTGGGCGGCTCTGC
  CGGCGCCGCCGGGGCCGAGGACAGCTACGGGCTGGTGGCTCCCGCCACGGCCGCCTACCG
  TGAGGTGTGGCAGGTGAACCTGAAGCCCAAGGGTCTGGGCCAGAGCAAGAACCTGACGGG
  GGTGTACCGTCTGTGCCTGTCTGCGCGCACCATCGGCTTCGTGAAGCTCAACTGCGAGCA
  GCCGTCGGTGACGCTGCAGCTCATGAACATCCGCCGCTGCGGCCACTCGGACAGCTTCTT
  CTTCATCGAGGTGGGCCGCTCGGCCGTCACAGGCCCCGGCGAGCTGTGGATGCAGGCGGA
  CGACTCGGTGGTGGCGCAGAACATCCACGAGACCATCCTGGAGGCCATGAAGGCGCTCAA
  GGAGCTCTTCGAGTTCCGGCCGCGCAGTAAGAGCCAATCGTCGGGGTCGTCGGCCACGCA
  CCCCATCAGCGTCCCCGGCGCGCGCCGCCACCACCACCTGGTCAACCTGCCCCCCAGCCA
  GACGGGCCTGGTGCGCCGCTCGCGCACCGACAGCCTGGCCGCCACCCCGCCGGCGGCCAA
  GTGCAGCTCGTGCCGGGTGCGCACCGCCAGCGAGGGCGACGGCGGCGCGGCGGCGGGAGC
  GGCGGCCGCGGGCGCCAGGCCGGTGTCGGTGGCTGGGAGCCCCCTGAGCCCCGGGCCGGT
  GCGCGCGCCCCTGAGCCGCTCGCACACCCTGAGCGGCGGCTGCGGCGGCCGCGGGAGCAA
  GGTGGCGCTGCTGCCGGCAGGGGGCGCGCTGCAACACAGCCGCTCCATGTCCATGCCCGT
  GGCGCACTCGCCGCCCGCCGCCACCAGCCCCGGCTCCCTGTCGTCCAGCAGCGGCCACGG
  CTCGGGCTCCTACCCGCCGCCGCCCGGCCCGCACCCGCCTCTGCCGCATCCGCTGCACCA
  CGGCCCCGGCCAGCGGCCCTCCAGCGGCAGCGCCTCCGCCTCGGGCTCCCCCAGCGACCC
  CGGCTTCATGTCCCTGGACGAGTACGGCTCCAGCCCAGGCGACCTGCGCGCCTTCTGCAG
  CCACCGAAGCAACACGCCCGAGTCCATCGCGGAGACGCCCCCGGCCCGAGACGGCGGCGG
  CGGCGGTGAGTTCTACGGGTACATGACCATGGACAGGCCCCTGAGCCACTGTGGCCGCTC
  CTACCGCCGGGTCTCGGGGGACGCGGCCCAGGACCTGGACCGAGGGCTGCGCAAGAGGAC
  CTACTCCCTGACCACGCCAGCCCGGCAGCGGCCGGTGCCCCAGCCCTCCTCTGCCTCGCT
  GGATGAATACACCCTGATGCGGGCCACCTTCTCGGGCAGCGCGGGCCGCCTCTGCCCGTC
  CTGCCCCGCGTCCTCTCCCAAGGTGGCCTACCACCCCTACCCAGAGGACTACGGAGACAT
  CGAGATCGGCTCCCACAGGAGCTCCAGCAGCAACCTGGGGGCAGACGACGGCTACATGCC
  CATGACGCCCGGCGCGGCCCTCGCGGGCAGTGGGAGCGGCAGCTGCAGGAGCGACGACTA
  CATGCCCATGAGCCCCGCCAGCGTGTCCGCCCCCAAGCAGATCTTGCAGCCCAGGGCCGC
  CGCCGCCGCCGCCGCCGCCGTGCCTTCTGCGGGGCCTGCGGGGCCAGCACCCACCTCTGC
  GGCGGGCAGGACATTCCCGGCGAGCGGGGGCGGCTACAAGGCCAGCTCGCCCGCCGAGAG
  CTCCCCCGAGGACAGTGGGTACATGCGCATGTGGTGCGGTTCCAAGCTGTCCATGGAGCA
  TGCAGATGGCAAGCTGCTGCCCAACGGGGACTACCTCAACGTGTCCCCCAGCGACGCGGT
  CACCACGGGCACCCCGCCCGACTTCTTCTCCGCAGCCCTGCACCCCGGCGGGGAGCCGCT
  CAGGGGCGTTCCCGGCTGCTGCTACAGCTCCTTGCCCCGCTCCTACAAGGCCCCCTACAC
  CTGTGGCGGGGACAGCGACCAGTACGTGCTCATGAGCTCCCCCGTGGGGCGCATCCTGGA
  GGAGGAGCGTCTGGAGCCTCAGGCCACGCCAGGGCCCAGCCAGGCGGCCAGCGCCTTCGG
  GGCCGGCCCCACGCAGCCCCCTCACCCTGTAGTGCCTTCGCCCGTGCGGCCTAGCGGCGG
  CAGCCCGGAGGGCTTCTTGGGCCAGCGCGGCCGGGCGGTGAGGCCCACGCGCCTGTCCCT
  GGAGGGGCTGCCCAGCCTGCCCAGCATGCACGAGTACCCACTGCCACCGGAGCCCAAGAG
  CCCCGGCGAGTACATCAACATCGACTTTGGCGAGCCCGGGGCCCGCCTGTCGCCGCCCGC
  GCCTCCCCTGCTGGCGTCGGCGGCCTCGTCCTCCTCGCTCTTGTCCGCCAGCAGCCCGGC
  CTCGTCGCTGGGCTCAGGCACCCCGGGCACCAGCAGCGACAGCCGGCAGCGGTCTCCGCT
  CTCCGACTACATGAACCTCGACTTCAGCTCCCCCAAGTCTCCTAAGCCGGGCGCCCCGAG
  CGGCCACCCCGTGGGCTCCTTGGACGGCCTCCTGTCCCCCGAGGCCTCCTCCCCGTATCC
  GCCGTTGCCCCCGCGTCCGTCCGCGTCCCCGTCGTCGTCTCTGCAGCCGCCGCCACCGCC
  GCCGGCCCCGGGGGAGCTGTACCGCCTGCCCCCCGCCTCGGCCGTTGCCACCGCCCAGGG
  CCCGGGCGCCGCCTCATCGTTGTCCTCGGACACCGGGGACAATGGTGACTACACCGAGAT
  GGCTTTTGGTGTGGCCGCCACCCCGCCGCAACCTATCGCGGCCCCCCCGAAGCCAGAAGC
  TGCCCGCGTGGCCAGCCCGACGTCGGGCGTGAAGAGGCTGAGCCTCATGGAGCAGGTGTC
  GGGAGTCGAGGCCTTCCTGCAGGCCAGCCAGCCCCCGGACCCCCACCGCGGCGCCAAGGT
  CATCCGCGCAGACCCGCAGGGGGGCCGCCGCCGCCACAGTTCCGAGACCTTCTCCTCCAC
  CACGACGGTCACCCCCGTGTCCCCGTCCTTCGCCCACAACCCCAAGCGCCACAACTCGGC
  CTCCGTGGAAAATGTCTCTCTCAGGAAAAGCAGCGAGGGCGGCGTGGGTGTCGGCCCTGG
  AGGGGGCGACGAGCCGCCCACCTCCCCACGACAGTTGCAGCCGGCGCCCCCTTTGGCACC
  GCAGGGCCGGCCGTGGACCCCGGGTCAGCCCGGGGGCTTGGTCGGTTGTCCTGGGAGCGG
  TGGATCGCCCATGCGCAGAGAGACCTCTGCCGGCTTCCAGAATGGTCTCAACTACATCGC
  CATCGACGTGAGGGAGGAGCCCGGGCTGCCACCCCAGCCGCAGCCGCCGCCGCCGCCGCT
  TCCTCAGCCGGGAGACAAGAGCTCCTGGGGCCGGACCCGAAGCCTCGGGGGTCTCATCAG
  CGCTGTGGGCGTCGGCAGCACCGGCGGCGGGTGCGGGGGGCCGGGTCCCGGTGCCCTGCC
  CCCTGCCAACACCTACGCCAGCATTGACTTCTTGTCCCACCACTTGAAGGAGGCCACCAT
  CGTGAAAGAGTGAAGATCTGTCTGGCTTTATCACCAGGATGTCACATGTCAGAGAATATC
  ATTAAAAGAAGACGCTCAGCACTGTTTCAGCCCGAAGCTGCTTGCAGTTTTCTTTTGGAT
  CTGAGCAATGACTGTGTTTGGAAACATCTGTGGACTCTGTTAGATGAGGCACCAACAAGG
  CAAGGTCACCTGCCTCTTTCCCTTGTTCCCGGATGGGGCATTCATCATTGTGCTGTTTGC
  GTTTTGTTTTGTTTTGTTTTAACAAAATTAGCTGAAGAAGTTATTCTCAAGAAAATTGGA
  TGTTTTCATTGGCCTTCTTAAATTGTGGCCAGTGTCTTTTAATTTCTTCTTCTTTTCCTT
  TTGGCAAAGCAGATATAACCCTCAGCATGCTAGGAGAGTGCACCCGTACCTATGGAAGTG
  GTAAAATCTGGTATTTACTGGCTTACACTCAAAACGACCACAGTCCTACCTCAGTTCAAG
  GTAAAGCCGGATTTCCGTGGCGGGGGTCCCACAGGACCTCCTGTAGTAGCCCCTGCGCTG
  TGTGTCTGGAGCGCGGTCCTCGGCCTTATTGAAATGGTCCAAGTAGACAGCTGCTTGTTG
  GATTCCAGTGCAGGTACCTGCGATGTTTACGTCCACACCGAGCCCAGTGTGGGACTGACA
  TTTCTCAATGGAAGTGAAATTTGGGATTGGACTTTGAAGACGGATTACTAAATAATAATT
  ATTATATGTAACTGAAGCAACCTACTTTTGAAAATCAACTGTATTGGGTAGTGGGAGGTG
  GGAGGGAAGGGCTTTGGGAAGGGGATGAATATTTTTTTTTACCTTTAACAGACTTGTTTA
  ATCTTCTCGATGTAGATGTTTATGTAGGTACTTCACATTGCAAACGCCTTTTATT
  
• Restriction Sites: Please inquire
• ACCN: NM_003749
• Insert Size: 4900 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003749.2, NP_003740.2
• RefSeq Size: 7014 bp
• RefSeq ORF: 4017 bp
• Locus ID: 8660
• UniProt ID: Q9Y4H2
• Cytogenetics: 13q34
• Protein Families: Druggable Genome
• Protein Pathways: Adipocytokine signaling pathway, Insulin signaling pathway, Neurotrophin signaling pathway, Type II diabetes mellitus
• Gene Summary: This gene encodes the insulin receptor substrate 2, a cytoplasmic signaling molecule that mediates effects of insulin, insulin-like growth factor 1, and other cytokines by acting as a molecular adaptor between diverse receptor tyrosine kinases and downstream effectors. The product of this gene is phosphorylated by the insulin receptor tyrosine kinase upon receptor stimulation, as well as by an interleukin 4 receptor-associated kinase in response to IL4 treatment. [provided by RefSeq, Jul 2008]