CCT6A (NM_001009186) Human Tagged ORF Clone
SKU
RC212330
CCT6A (Myc-DDK-tagged)-Human chaperonin containing TCP1, subunit 6A (zeta 1) (CCT6A), transcript variant 2
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Protein expression verified by Western blot, fully sequenced and in stock
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Product Data | |
Type | Human Tagged ORF Clone |
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Target Symbol | CCT6A |
Synonyms | CCT-zeta; CCT-zeta-1; CCT6; Cctz; HTR3; MoDP-2; TCP-1-zeta; TCP20; TCPZ; TTCP20 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
ORF Nucleotide Sequence
>RC212330 representing NM_001009186
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCGGTGAAGACCCTGAACCCCAAGGCCGAGGTGGCCCGAGCGCAGGCGGCGCTGGCGGTCAACA TCAGCGCAGCGCGGGGTCTGCAGGACGTGCTAAGGACCAACCTGGGGCCCAAGGGCACCATGAAGATGCT CGTTTCTGGCGCTGGAGACATCAAACTTACTAAAGACGGCAATGTGCTGCTTCACGAAATGGGCCTTCAT CCTAGAATAATCACTGAAGGATTTGAAGCTGCAAAGGAAAAGGCCCTTCAGTTTTTGGAAGAAGTCAAAG TAAGCAGAGAGATGGACAGGGAAACACTTATAGATGTGGCCAGAACATCTCTTCGTACTAAAGTTCATGC TGAACTTGCAGATGTCTTAACAGAGGCTGTAGTGGACTCCATTTTGGCCATTAAAAAGCAAGATGAACCT ATTGATCTCTTCATGATTGAGATCATGGAGATGAAACATAAATCTGAAACTGATACAAGCTTAATCAGAG GGCTTGTTTTGGACCACGGAGCACGGCATCCTGATATGAAGAAAAGGGTGGAGGATGCATACATCCTCAC TTGTAACGTGTCATTAGAGTATGAGAAAACAGAAGTGAATTCTGGCTTTTTTTACAAGAGTGCAGAAGAG AGAGAAAAACTCGTGAAAGCTGAAAGAAAATTCATTGAAGATAGGGTTAAAAAAATAATAGAACTGAAAA GGAAAGTCTGTGGCGATTCAGATAAAGGATTTGTTGTTATTAATCAAAAGGGAATTGACCCCTTTTCCTT AGATGCTCTTTCAAAAGAAGGCATAGTTGCTCTGCGCAGAGCTAAAAGGAGAAATATGGAGAGGCTGACT CTTGCTTGTGGTGGGGTAGCCCTGAATTCTTTTGACGACCTAAGTCCTGACTGCTTGGGACATGCAGGAC TTGTATATGAGTATACATTGGGAGAAGAGAAGTTTACCTTTATTGAGAAATGTAACAACCCTCGTTCTGT CACATTATTGATCAAAGGACCAAATAAGCACACACTCACTCAGATCAAAGATGCAGTGAGGGACGGCTTG AGGGCTGTCAAAAATGCTATTGATGATGGCTGTGTGGTTCCAGGTGCTGGTGCCGTGGAAGTGGCAATGG CAGAAGCCCTGATTAAACATAAGCCCAGTGTAAAGGGCAGGGCACAGCTTGGAGTCCAAGCATTTGCTGA TGCATTGCTCATTATTCCCAAGGTTCTTGCTCAGAACTCTGGTTTTGACCTTCAGGAAACATTAGTTAAA ATTCAAGCAGAACATTCAGAATCAGGTCAGCTTGTGGGTGTGGACCTGAACACAGGTGAGCCAATGGTGG CAGCAGAAGTAGGCGTATGGGATAACTATTGTGTAAAGAAACAGCTTCTTCACTCCTGCACTGTGATTGC CACCAACATTCTCTTGGTTGATGAGATCATGCGAGCTGGAATGTCTTCTCTGAAAGGT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC212330 representing NM_001009186
Red=Cloning site Green=Tags(s) MAAVKTLNPKAEVARAQAALAVNISAARGLQDVLRTNLGPKGTMKMLVSGAGDIKLTKDGNVLLHEMGLH PRIITEGFEAAKEKALQFLEEVKVSREMDRETLIDVARTSLRTKVHAELADVLTEAVVDSILAIKKQDEP IDLFMIEIMEMKHKSETDTSLIRGLVLDHGARHPDMKKRVEDAYILTCNVSLEYEKTEVNSGFFYKSAEE REKLVKAERKFIEDRVKKIIELKRKVCGDSDKGFVVINQKGIDPFSLDALSKEGIVALRRAKRRNMERLT LACGGVALNSFDDLSPDCLGHAGLVYEYTLGEEKFTFIEKCNNPRSVTLLIKGPNKHTLTQIKDAVRDGL RAVKNAIDDGCVVPGAGAVEVAMAEALIKHKPSVKGRAQLGVQAFADALLIIPKVLAQNSGFDLQETLVK IQAEHSESGQLVGVDLNTGEPMVAAEVGVWDNYCVKKQLLHSCTVIATNILLVDEIMRAGMSSLKG TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
Chromatograms
![]() Sequencher program is needed, download here |
Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001009186 |
ORF Size | 1458 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Shipping | Ambient |
Reference Data | |
RefSeq | NM_001009186.2 |
RefSeq Size | 2547 bp |
RefSeq ORF | 1461 bp |
Locus ID | 908 |
UniProt ID | P40227 |
Cytogenetics | 7p11.2 |
MW | 53.1 kDa |
Summary | The protein encoded by this gene is a molecular chaperone that is a member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin. Alternate transcriptional splice variants of this gene, encoding different isoforms, have been characterized. In addition, several pseudogenes of this gene have been located. [provided by RefSeq, Jun 2010] |
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Product Manuals |
FAQs |
SDS |
SKU | Description | Size | Price | |
---|---|---|---|---|
RC212330L3 | Lenti ORF clone of Human chaperonin containing TCP1, subunit 6A (zeta 1) (CCT6A), transcript variant 2, Myc-DDK-tagged | 10 ug |
$757.00
|
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RC212330L4 | Lenti ORF clone of Human chaperonin containing TCP1, subunit 6A (zeta 1) (CCT6A), transcript variant 2, mGFP tagged | 10 ug |
$757.00
|
|
RG212330 | CCT6A (tGFP-tagged) - Human chaperonin containing TCP1, subunit 6A (zeta 1) (CCT6A), transcript variant 2 | 10 ug |
$657.00
|
|
SC301398 | CCT6A (untagged)-Human chaperonin containing TCP1, subunit 6A (zeta 1) (CCT6A), transcript variant 2 | 10 ug |
$503.00
|
|
Citations
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.