CEP170 (NM_014812) Human Tagged ORF Clone

SKU RC212249

CEP170 (Myc-DDK-tagged)-Human centrosomal protein 170kDa (CEP170), transcript variant alpha

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: CEP170
• Synonyms: FAM68A; KAB; KIAA0470
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC212249 representing NM_014812; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGAGCTTAACATCCTGGTTTTTGGTGAGCAGTGGAGGCACTCGCCACAGGCTGCCACGAGAAATGATTT; TTGTTGGAAGAGATGACTGTGAGCTCATGTTGCAGTCTCGTAGTGTGGATAAGCAACACGCTGTCATCAA; CTATGATGCGTCTACGGATGAGCATTTAGTGAAGGATTTGGGCAGCCTCAATGGGACTTTTGTGAATGAT; GTAAGGATTCCGGAACAGACTTATATCACCTTGAAACTTGAAGATAAGCTGAGATTTGGATATGATACAA; ATCTTTTCACTGTAGTACAAGGAGAAATGAGGGTCCCTGAAGAAGCTCTTAAGCATGAGAAGTTTACCAT; TCAGCTTCAGTTGTCCCAAAAATCTTCAGAATCAGAATTATCCAAATCTGCAAGTGCCAAAAGCATAGAT; TCAAAGGTAGCAGACGCTGCTACTGAAGTGCAGCACAAAACTACTGAAGCACTGAAATCCGAGGAAAAAG; CCATGGATATTTCTGCTATGCCCCGTGGTACTCCATTATATGGGCAGCCGTCATGGTGGGGGGATGATGA; GGTGGATGAAAAAAGAGCTTTCAAGACAAATGGCAAACCTGAAGAAAAAAACCATGAAGCTGGAACATCA; GGGTGCGGCATAGATGCCAAGCAAGTTGAGGAACAATCTGCAGCTGCAAATGAAGAAGTACTTTTTCCTT; TCTGTAGGGAACCAAGTTATTTTGAAATCCCTACAAAAGAATTCCAGCAACCATCACAAATAACAGAAAG; CACTATTCATGAAATCCCAACAAAAGACACGCCAAGTTCCCATATAACAGGTGCAGGGCATGCTTCATTT; ACCATTGAATTTGATGACAGTACCCCAGGGAAGGTAACTATTAGAGACCATGTGACAAAGTTTACTTCTG; ATCAGCGCCACAAGTCCAAGAAGTCTTCTCCTGGAACTCAAGACTTGCTGGGGATTCAAACAGGAATGAT; GGCACCCGAAAACAAAGTTGCTGACTGGCTAGCACAAAACAACCCTCCTCAAATGCTATGGGAAAGAACA; GAAGAGGATTCTAAAAGCATTAAAAGTGATGTTCCAGTGTACTTGAAAAGGTTGAAAGGAAATAAACATG; ATGATGGTACGCAAAGTGATTCAGAGAACGCTGGGGCTCACAGGCGCTGTAGCAAACGTGCAACTCTTGA; GGAACACTTAAGACGCCACCATTCAGAACACAAAAAGCTACAGAAGGTCCAGGCTACTGAAAAGCATCAA; GACCAAGCTGTTACTAGCTCTGCGCATCACAGAGGGGGGCATGGTGTTCCACATGGGAAATTGTTAAAAC; AGAAATCAGAGGAGCCATCGGTGTCAATACCCTTCCTACAAACTGCATTATTAAGAAGTTCAGGGAGTCT; TGGGCACAGACCAAGCCAGGAGATGGATAAAATGTTAAAAAATCAAGCAACTTCTGCTACTTCTGAAAAG; GATAATGATGATGACCAAAGTGACAAGGGTACTTATACCATTGAGTTAGAGAATCCCAACAGTGAGGAAG; TGGAAGCAAGAAAAATGATTGACAAGGTGTTTGGAGTAGATGACAATCAGGATTATAATAGGCCTGTTAT; CAACGAAAAACATAAAGATCTAATAAAAGATTGGGCTCTCAGTTCTGCTGCAGCAGTAATGGAAGAAAGA; AAACCACTGACTACATCTGGATTTCACCACTCAGAGGAAGGCACATCTTCATCTGGAAGCAAACGTTGGG; TTTCACAGTGGGCTAGTTTGGCTGCCAATCATACAAGGCATGATCAAGAAGAAAGGATAATGGAATTTTC; TGCACCTCTTCCTTTAGAGAATGAGACAGAGATCAGTGAGTCTGGCATGACAGTGAGAAGTACTGGCTCT; GCAACTTCCTTGGCTAGCCAGGGAGAGAGAAGGAGACGAACTCTTCCCCAGCTTCCAAATGAAGAAAAGT; CTCTTGAGAGCCACAGAGCAAAGGTTGTAACACAGAGGTCAGAGATAGGAGAAAAACAAGACACAGAACT; TCAGGAGAAAGAAACACCTACACAGGTATACCAGAAAGATAAACAAGATGCTGACAGACCCTTGAGTAAA; ATGAACAGGGCAGTAAATGGAGAGACTCTCAAAACTGGTGGAGATAATAAAACCCTACTTCACTTAGGCA; GCTCTGCTCCTGGAAAAGAGAAAAGTGAAACTGATAAGGAAACTTCTTTGGTAAAGCAAACATTAGCAAA; ACTTCAACAACAAGAACAAAGGGAGGAGGCTCAGTGGACACCTACTAAATTGTCTTCCAAAAATGTTTCA; GGTCAGACAGATAAATGTAGGGAGGAAACTTTTAAACAAGAATCACAACCTCCAGAAAAAAATTCAGGAC; ATTCTACAAGCAAAGGAGACAGAGTGGCACAAAGTGAGAGCAAGAGAAGAAAAGCTGAGGAAATTCTGAA; AAGTCAGACTCCAAAGGGAGGAGACAAGAAGGAATCCTCCAAGTCATTAGTGCGACAAGGGAGCTTCACT; ATAGAAAAACCCAGCCCAAACATACCCATAGAACTTATTCCCCATATAAATAAACAGACTTCCTCTACTC; CTTCTTCTTTAGCATTAACATCTGCAAGTAGAATACGAGAAAGAAGTGAGTCTTTGGATCCTGATTCTAG; TATGGACACAACCCTTATTCTAAAAGACACAGAAGCAGTAATGGCTTTTCTAGAAGCTAAACTACGTGAA; GATAATAAAACTGATGAAGGACCAGATACTCCCAGTTATAATAGAGACAATTCTATTTCACCAGAATCTG; ATGTAGATACAGCTAGTACAATCAGTCTGGTTACTGGAGAAACTGAAAGAAAGTCAACCCAAAAGCGAAA; GAGTTTCACTAGCCTCTATAAAGATAGGTGTTCCACAGGTTCTCCTTCCAAAGATGTTACAAAATCATCA; TCTTCAGGTGCTAGGGAAAAAATGGAAAAGAAAACAAAAAGTCGTTCCACAGATGTGGGTTCAAGAGCAG; ATGGTCGTAAATTTGTTCAGTCCAGTGGGAGAATAAGACAGCCCTCAGTAGACTTAACAGATGATGACCA; AACCTCTAGTGTACCTCATTCTGCCATCTCTGATATTATGTCATCTGATCAAGAAACTTACTCTTGTAAA; CCTCATGGACGGACTCCACTTACCTCAGCTGATGAGCATGTACATTCCAAACTGGAAGGAAGTAAAGTAA; CGAAATCTAAGACTTCTCCGGTGGTATCTGGTTCATCTAGTAAATCAACCACCCTTCCAAGGCCACGACC; TACCAGGACTTCCCTCTTGCGCAGAGCACGACTTGGTGAAGCTTCAGACAGTGAACTTGCTGATGCTGAC; AAAGCATCTGTTGCTTCTGAAGTATCCACAACAAGTTCTACATCAAAACCTCCCACAGGAAGGCGTAACA; TCTCTCGGATTGATTTATTGGCTCAGCCTCGTAGAACACGACTTGGCTCACTGTCAGCTCGTAGTGACTC; TGAAGCAACAATTTCTAGAAGTAGTGCCTCTTCGAGGACCGCAGAAGCCATCATTAGAAGTGGAGCCAGA; CTAGTACCATCAGATAAATTTTCTCCTAGAATTAGAGCTAACAGTATCTCTCGACTCTCAGACTCCAAGG; TCAAAAGTATGACCTCAGCTCATGGCTCTGCTTCAGTAAATTCAAGATGGAGGCGCTTTCCTACTGATTA; TGCTTCCACCTCAGAAGATGAATTTGGATCAAACCGTAATTCCCCTAAACATACCCGTCTACGTACTTCT; CCAGCCCTGAAAACCACTCGCTTGCAGAGCGCTGGATCAGCAATGCCTACTAGTTCTTCATTCAAACACC; GGATTAAAGAGCAGGAAGACTACATCCGAGATTGGACTGCTCATCGAGAAGAGATAGCCAGGATCAGCCA; AGATCTTGCTCTCATTGCTCGGGAGATCAACGATGTAGCAGGAGAGATAGATTCAGTGACTTCATCAGGC; ACTGCCCCTAGTACCACAGTAAGCACTGCTGCCACCACCCCTGGCTCTGCCATAGACACTAGAGAAGAGT; TGGTTGATCGTGTTTTTGATGAAAGCCTCAACTTCCGAAAGATTCCTCCATTAGTTCATTCCAAAACACC; AGAAGGAAACAACGGTCGATCTGGTGATCCAAGACCTCAAGCAGCAGAGCCTCCCGATCACTTAACAATT; ACAAGGCGGAGAACCTGGAGCAGGGATGAAGTCATGGGAGATAATCTGCTGCTGTCATCCGTCTTTCAGT; TCTCTAAGAAGATAAGACAATCTATAGATAAGACAGCTGGAAAGATCAGAATATTATTTAAAGACAAAGA; TCGGAATTGGGATGACATAGAAAGCAAATTAAGAGCCGAAAGTGAAGTCCCTATTGTGAAAACCTCAAGC; ATGGAGATTTCTTCTATCTTACAGGAACTGAAAAGAGTAGAAAAGCAGCTGCAAGCAATCAATGCTATGA; TTGATCCTGATGGAACTTTGGAGGCTCTGAACAACATGGGATTTCCCAGTGCTATGTTGCCATCTCCACC; GAAACAGAAGTCCAGCCCTGTGAATAACCACCACAGCCCGGGTCAGACACCAACACTTGGCCAACCAGAA; GCTAGGGCTCTTCATCCTGCTGCTGTTTCAGCCGCAGCTGAATTTGAGAATGCTGAATCTGAGGCTGATT; TCAGTATACATTTCAATAGATTCAACCCCGATGGGGAAGAGGAAGATGTTACAGTACAAGAA; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC212249 representing NM_014812; Red=Cloning site Green=Tags(s); ; MSLTSWFLVSSGGTRHRLPREMIFVGRDDCELMLQSRSVDKQHAVINYDASTDEHLVKDLGSLNGTFVND; VRIPEQTYITLKLEDKLRFGYDTNLFTVVQGEMRVPEEALKHEKFTIQLQLSQKSSESELSKSASAKSID; SKVADAATEVQHKTTEALKSEEKAMDISAMPRGTPLYGQPSWWGDDEVDEKRAFKTNGKPEEKNHEAGTS; GCGIDAKQVEEQSAAANEEVLFPFCREPSYFEIPTKEFQQPSQITESTIHEIPTKDTPSSHITGAGHASF; TIEFDDSTPGKVTIRDHVTKFTSDQRHKSKKSSPGTQDLLGIQTGMMAPENKVADWLAQNNPPQMLWERT; EEDSKSIKSDVPVYLKRLKGNKHDDGTQSDSENAGAHRRCSKRATLEEHLRRHHSEHKKLQKVQATEKHQ; DQAVTSSAHHRGGHGVPHGKLLKQKSEEPSVSIPFLQTALLRSSGSLGHRPSQEMDKMLKNQATSATSEK; DNDDDQSDKGTYTIELENPNSEEVEARKMIDKVFGVDDNQDYNRPVINEKHKDLIKDWALSSAAAVMEER; KPLTTSGFHHSEEGTSSSGSKRWVSQWASLAANHTRHDQEERIMEFSAPLPLENETEISESGMTVRSTGS; ATSLASQGERRRRTLPQLPNEEKSLESHRAKVVTQRSEIGEKQDTELQEKETPTQVYQKDKQDADRPLSK; MNRAVNGETLKTGGDNKTLLHLGSSAPGKEKSETDKETSLVKQTLAKLQQQEQREEAQWTPTKLSSKNVS; GQTDKCREETFKQESQPPEKNSGHSTSKGDRVAQSESKRRKAEEILKSQTPKGGDKKESSKSLVRQGSFT; IEKPSPNIPIELIPHINKQTSSTPSSLALTSASRIRERSESLDPDSSMDTTLILKDTEAVMAFLEAKLRE; DNKTDEGPDTPSYNRDNSISPESDVDTASTISLVTGETERKSTQKRKSFTSLYKDRCSTGSPSKDVTKSS; SSGAREKMEKKTKSRSTDVGSRADGRKFVQSSGRIRQPSVDLTDDDQTSSVPHSAISDIMSSDQETYSCK; PHGRTPLTSADEHVHSKLEGSKVTKSKTSPVVSGSSSKSTTLPRPRPTRTSLLRRARLGEASDSELADAD; KASVASEVSTTSSTSKPPTGRRNISRIDLLAQPRRTRLGSLSARSDSEATISRSSASSRTAEAIIRSGAR; LVPSDKFSPRIRANSISRLSDSKVKSMTSAHGSASVNSRWRRFPTDYASTSEDEFGSNRNSPKHTRLRTS; PALKTTRLQSAGSAMPTSSSFKHRIKEQEDYIRDWTAHREEIARISQDLALIAREINDVAGEIDSVTSSG; TAPSTTVSTAATTPGSAIDTREELVDRVFDESLNFRKIPPLVHSKTPEGNNGRSGDPRPQAAEPPDHLTI; TRRRTWSRDEVMGDNLLLSSVFQFSKKIRQSIDKTAGKIRILFKDKDRNWDDIESKLRAESEVPIVKTSS; MEISSILQELKRVEKQLQAINAMIDPDGTLEALNNMGFPSAMLPSPPKQKSSPVNNHHSPGQTPTLGQPE; ARALHPAAVSAAAEFENAESEADFSIHFNRFNPDGEEEDVTVQE; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_014812
• ORF Size: 4752 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.; ; The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_014812.3
• RefSeq Size: 7204 bp
• RefSeq ORF: 4755 bp
• Locus ID: 9859
• UniProt ID: Q5SW79
• Cytogenetics: 1q43
• Domains: FHA
• MW: 175.3 kDa
• Summary: The product of this gene is a component of the centrosome, a non-membraneous organelle that functions as the major microtubule-organizing center in animal cells. During interphase, the encoded protein localizes to the sub-distal appendages of mature centrioles, which are microtubule-based structures thought to help organize centrosomes. During mitosis, the protein associates with spindle microtubules near the centrosomes. The protein interacts with and is phosphorylated by polo-like kinase 1, and functions in maintaining microtubule organization and cell morphology. The human genome contains a putative transcribed pseudogene. Several alternatively spliced transcript variants of this gene have been found, but the full-length nature of some of these variants has not been determined. [provided by RefSeq, Jul 2008]