RGS4 (NM_001102445) Human Tagged ORF Clone
SKU
RC212133
RGS4 (Myc-DDK-tagged)-Human regulator of G-protein signaling 4 (RGS4), transcript variant 1
$300.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | RGS4 |
| Synonyms | RGP4; SCZD9 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_001102445 |
| ORF Size | 906 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_001102445.2, NP_001095915.1 |
| RefSeq Size | 3215 bp |
| RefSeq ORF | 909 bp |
| Locus ID | 5999 |
| UniProt ID | P49798, A7XA59 |
| Cytogenetics | 1q23.3 |
| Protein Families | Druggable Genome |
| MW | 33.7 kDa |
| Summary | Regulator of G protein signaling (RGS) family members are regulatory molecules that act as GTPase activating proteins (GAPs) for G alpha subunits of heterotrimeric G proteins. RGS proteins are able to deactivate G protein subunits of the Gi alpha, Go alpha and Gq alpha subtypes. They drive G proteins into their inactive GDP-bound forms. Regulator of G protein signaling 4 belongs to this family. All RGS proteins share a conserved 120-amino acid sequence termed the RGS domain. Regulator of G protein signaling 4 protein is 37% identical to RGS1 and 97% identical to rat Rgs4. This protein negatively regulate signaling upstream or at the level of the heterotrimeric G protein and is localized in the cytoplasm. Alternatively spliced transcript variants have been found for this gene. provided by RefSeq, Jul 2008 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_001102445" in other vectors (4)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC212133L3 | Lenti ORF clone of Human regulator of G-protein signaling 4 (RGS4), transcript variant 1, Myc-DDK-tagged | 10 ug |
$600.00
|
|
| RC212133L4 | Lenti ORF clone of Human regulator of G-protein signaling 4 (RGS4), transcript variant 1, mGFP tagged | 10 ug |
$600.00
|
|
| RG212133 | RGS4 (tGFP-tagged) - Human regulator of G-protein signaling 4 (RGS4), transcript variant 1 | 10 ug |
$500.00
|
|
| SC316920 | RGS4 (untagged)-Human regulator of G-protein signaling 4 (RGS4), transcript variant 1 | 10 ug |
$330.00
|
|
Citations
G protein signaling-biased agonism at the κ-opioid receptor is maintained in striatal neurons
Ho, Stahl, Schmid et al
Sci Signal (2018) 11 (542) DOI: 10.1126/scisignal.aar4309
Ho, Stahl, Schmid et al
Sci Signal (2018) 11 (542) DOI: 10.1126/scisignal.aar4309
AI Summary: OriGene contributed human GIRK1, hRGS4, hRGS9.2, and hRGS12.3 cDNA products, which were used to study signaling pathways involved in κ-opioid receptor biased agonism in striatal neurons.
Snippet: … Human GIRK1 (hGIRK1), human RGS4 (hRGS4), human RGS9 variant 2 (hRGS9.2), and human RGS 12 variant 3 (hRGS12.3) cDNA were purchased from OriGene, and hGIRK2 cDNA was purchased from GE Life Sciences. For 3×HA-hKOR-hGIRK1-hGIRK2, 3×HA-hKOR-Myc-hRGS4, or −9.2 or −12.3 constructs, the cDNA of each gene (cDNA were purchased from cDNA.org.)
Growth inhibition of human breast carcinoma cells by overexpression of regulator of G-protein signaling 4
PARK, KIM, MOON
Oncology Letters (2017) 13, 4357
PARK, KIM, MOON
Oncology Letters (2017) 13, 4357
Showing 1-2 of 2 papers.
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