PCDHGB4 (NM_032098) Human Tagged ORF Clone

SKU RC211321

PCDHGB4 (Myc-DDK-tagged)-Human protocadherin gamma subfamily B, 4 (PCDHGB4), transcript variant 2

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: PCDHGB4
• Synonyms: CDH20; FIB2; PCDH-GAMMA-B4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC211321 ORF sequence; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGGGAGCGGCGCCGGGGAGCTGGGCCGGGCTGAGAGGCTGCCAGTGCTCTTTCTCTTCCTGCTGTCTT; TGTTCTGCCCGGCGCTCTGTGAGCAGATCCGCTACAGGATTCCCGAGGAAATGCCCAAGGGCTCCGTAGT; GGGGAACCTCGCCACGGACCTGGGGTTCAGCGTCCAGGAGTTACCGACTCGAAAACTGCGCGTCAGTTCG; GAGAAGCCTTACTTCACCGTGAGCGCAGAGAGCGGGGAGTTGCTTGTGAGCAGCAGGCTAGACAGGGAGG; AGATATGCGGGAAGAAGCCAGCTTGTGCTCTGGAATTTGAGGCTGTTGCTGAAAATCCACTGAACTTTTA; TCACGTGAATGTGGAGATCGAGGACATTAATGACCACACGCCAAAATTCACGCAAAATTCCTTTGAGCTG; CAAATAAGTGAGTCTGCACAGCCTGGCACACGATTTATATTAGGATCTGCCCATGATGCGGATATTGGTA; GCAACACACTGCAGAATTACCAACTCAGTCCCAGTGATCATTTCTCACTGATAAATAAAGAGAAATCAGA; TGGCAGTAAATACCCTGAGATGGTATTGAAGACACCTTTGGACAGAGAAAAGCAGAAATCCTACCACTTG; ACTTTGACTGCCTTGGACTTTGGAGCTCCACCCCTAAGCAGCACTGCACAGATACACGTTCTAGTGACTG; ATGCCAATGATAATGCTCCAGTGTTCAGTCAAGACGTATACAGGGTGAGCCTTTCAGAAAACGTGTACCC; GGGGACCACGGTGCTACAGGTGACTGCCACGGACCAGGATGAGGGTGTCAATGCCGAGATTACTTTCTCT; TTCAGTGAAGCTAGCCAGATCACCCAATTTGACCTGAACTCTAACACCGGGGAAATTACTGTTTTAAATA; CATTAGATTTTGAAGAAGTCAAAGAATATTCCATAGTTTTGGAAGCAAGGGACGGTGGAGGAATGATTGC; GCAATGCACAGTGGAGGTAGAAGTCATAGATGAAAATGACAACGCCCCAGAAGTGATATTCCAGTCTCTA; CCCAACCTAATTATGGAGGACGCCGAGCTGGGAACACATATTGCTTTGCTCAAAGTCCGTGACAAGGATT; CCAGACACAATGGAGAAGTGACTTGTAAATTGGAAGGTGATGTTCCATTTAAAATATTAACTTCTTCAAG; AAACACGTATAAATTAGTGACAGATGCTGTTCTAGACCGCGAGCAGAATCCAGAGTACAATATAACCGTT; ACGGCAACAGATCGGGGCAAGCCTCCCCTCTCCTCCAGTTCCAGCATCACCCTGCACATTGGTGATGTAA; ATGACAACGCTCCGGTTTTCTCACAGTCTTCCTATATAGTCCACGTGGCCGAGAACAACCCGCCTGGAGC; CTCTATTTCACAAGTCAGGGCTTCTGATCCGGACTTGGGGCCCAACGGCCAAGTCTCTTACTGCATCATG; GCCAGTGACCTGGAGCAGCGGGAGCTGTCATCCTACGTGTCCATAAGCGCGGAGAGCGGGGTGGTGTTCG; CGCAGCGCGCCTTCGACCACGAGCAGCTGCGCGCCTTCGAACTCACACTGCAGGCCCGCGACCAGGGCTC; GCCAGCGCTCAGCGCGAACGTGAGCCTGCGCGTGTTAGTGGACGACCGCAACGACAATGCGCCACGGGTG; CTGTACCCCGCGCTGGGTCCCGACGGCTCTGCGCTCTTCGATATGGTGCCGCACGCTGCAGAGCCTGGCT; ACTTGGTGACCAAGGTAGTGGCGGTGGACGCAGACTCAGGACACAACGCCTGGCTGTCCTACCACGTGCT; GCAGGCTAGCGAGCCCGGGCTCTTCAGCCTGGGGCTGCGCACGGGCGAAGTGCGCACAGCGCGTGCCTTA; GGCGACAGGGACGCCGTCCGCCAGCGCCTTCTGGTCGCCGTGCGTGACGGTGGACAGCCACCACTCTCGG; CCACTGCCACGTTGCACCTGGTCTTCGCCGACAGCTTGCAGGAGGTGCTGCCGGATATCACTGACCGCCC; CGACCCCTCTGACCTCCAGGCTGAGCTGCAGTTTTACCTAGTGGTGGCCTTGGCCTTGATCTCAGTGCTC; TTCCTCGTGGCCATGATTCTGGCCATTGCCTTGCGCCTGCGACGCTCCTCCAGCCCCGCCTCCTGGAGCT; GCTTCCAGCCTGGTCTCTGTGTTAAATCCGAATCCGTGGTTCCCCCCAACTACAGCGAGGGGACTTTGCC; TTATTCCTACAATCTATGTGTTGCACATACAGGAAAGACGGAGTTTAATTTCCTAAAATGTAGTGAGCAG; TTGAGTTCAGGACAAGACATACTTTGCGGTGATTCATCTGGGGCCTTATTTCCACTTTGTAATTCCAGTG; AATTGACTTCCCATCAGGTGAGTTTCCTT; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC211321 protein sequence; Red=Cloning site Green=Tags(s); ; MGSGAGELGRAERLPVLFLFLLSLFCPALCEQIRYRIPEEMPKGSVVGNLATDLGFSVQELPTRKLRVSS; EKPYFTVSAESGELLVSSRLDREEICGKKPACALEFEAVAENPLNFYHVNVEIEDINDHTPKFTQNSFEL; QISESAQPGTRFILGSAHDADIGSNTLQNYQLSPSDHFSLINKEKSDGSKYPEMVLKTPLDREKQKSYHL; TLTALDFGAPPLSSTAQIHVLVTDANDNAPVFSQDVYRVSLSENVYPGTTVLQVTATDQDEGVNAEITFS; FSEASQITQFDLNSNTGEITVLNTLDFEEVKEYSIVLEARDGGGMIAQCTVEVEVIDENDNAPEVIFQSL; PNLIMEDAELGTHIALLKVRDKDSRHNGEVTCKLEGDVPFKILTSSRNTYKLVTDAVLDREQNPEYNITV; TATDRGKPPLSSSSSITLHIGDVNDNAPVFSQSSYIVHVAENNPPGASISQVRASDPDLGPNGQVSYCIM; ASDLEQRELSSYVSISAESGVVFAQRAFDHEQLRAFELTLQARDQGSPALSANVSLRVLVDDRNDNAPRV; LYPALGPDGSALFDMVPHAAEPGYLVTKVVAVDADSGHNAWLSYHVLQASEPGLFSLGLRTGEVRTARAL; GDRDAVRQRLLVAVRDGGQPPLSATATLHLVFADSLQEVLPDITDRPDPSDLQAELQFYLVVALALISVL; FLVAMILAIALRLRRSSSPASWSCFQPGLCVKSESVVPPNYSEGTLPYSYNLCVAHTGKTEFNFLKCSEQ; LSSGQDILCGDSSGALFPLCNSSELTSHQVSFL; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Chromatograms: Chromatograms ; Sequencher program is needed, download here
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_032098
• ORF Size: 2409 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_032098.1, NP_115269.1
• RefSeq Size: 2412 bp
• RefSeq ORF: 2412 bp
• Locus ID: 8641
• UniProt ID: Q9UN71
• Cytogenetics: 5q31.3
• MW: 87.4 kDa
• Summary: This gene is a member of the protocadherin gamma gene cluster, one of three related clusters tandemly linked on chromosome five. These gene clusters have an immunoglobulin-like organization, suggesting that a novel mechanism may be involved in their regulation and expression. The gamma gene cluster includes 22 genes divided into 3 subfamilies. Subfamily A contains 12 genes, subfamily B contains 7 genes and 2 pseudogenes, and the more distantly related subfamily C contains 3 genes. The tandem array of 22 large, variable region exons are followed by a constant region, containing 3 exons shared by all genes in the cluster. Each variable region exon encodes the extracellular region, which includes 6 cadherin ectodomains and a transmembrane region. The constant region exons encode the common cytoplasmic region. These neural cadherin-like cell adhesion proteins most likely play a critical role in the establishment and function of specific cell-cell connections in the brain. This particular family member is expressed in fibroblasts and is thought to play a role in wound healing in response to injury. Alternative splicing has been described for the gamma cluster genes. [provided by RefSeq, Jul 2008]