TRPV3 (NM_145068) Human Tagged ORF Clone

SKU RC211184

TRPV3 (Myc-DDK-tagged)-Human transient receptor potential cation channel, subfamily V, member 3 (TRPV3)

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: TRPV3
• Synonyms: FNEPPK2; OLMS; OLMS1; VRL3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC211184 ORF sequence; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGAAAGCCCACCCCAAGGAGATGGTGCCTCTCATGGGCAAGAGAGTTGCTGCCCCCAGTGGGAACCCTG; CCGTCCTGCCAGAGAAGAGGCCGGCGGAGATCACCCCCACAAAGAAGAGTGCACACTTCTTCCTGGAGAT; AGAAGGGTTTGAACCCAACCCCACAGTTGCCAAGACCTCTCCTCCTGTCTTCTCCAAGCCCATGGATTCC; AACATCCGGCAGTGCATCTCTGGTAACTGTGATGACATGGACTCCCCCCAGTCTCCTCAAGATGATGTGA; CAGAGACCCCATCCAATCCCAACAGCCCCAGTGCACAGCTGGCCAAGGAAGAGCAGAGGAGGAAAAAGGG; GCGGCTGAAGAAGCGCATCTTTGCAGCCGTGTCTGAGGGCTGCGTGGAGGAGTTGGTAGAGTTGCTGGTG; GAGCTGCAGGAGCTTTGCAGGCGGCGCCATGATGAGGATGTGCCTGACTTCCTCATGCACAAGCTGACGG; CCTCCGACACGGGGAAGACCTGCCTGATGAAGGCCTTGTTAAACATCAACCCCAACACCAAGGAGATCGT; GCGGATCCTGCTTGCCTTTGCTGAAGAGAACAACATCCTGGGCAGGTTCATCAACGCCGAGTACACAGAG; GAGGCCTATGAAGGGCAGACGGCGCTGAACATCGCCATCGAGCGGCGGCAGGGGGACATCGCAGCCCTGC; TCATCGCCGCCGGCGCCGACGTCAACGCGCACGCCAAGGGGGCCTTCTTCAACCCCAAGTACCAACACGA; AGGCTTCTACTTCGGTGAGACGCCCCTGGCCCTGGCAGCATGCACCAACCAGCCCGAGATTGTGCAGCTG; CTGATGGAGCACGAGCAGACGGACATCACCTCGCGGGACTCACGAGGCAACAACATCCTTCACGCCCTGG; TGACCGTGGCCGAGGACTTCAAGACGCAGAATGACTTTGTGAAGCGCATGTACGACATGATCCTACTGCG; GAGTGGCAACTGGGAGCTGGAGACCACTCGCAACAACGATGGCCTCACGCCGCTGCAGCTGGCCGCCAAG; ATGGGCAAGGCGGAGATCCTGAAGTACATCCTCAGTCGTGAGATCAAGGAGAAGCGGCTCCGGAGCCTGT; CCAGGAAGTTCACCGACTGGGCGTACGGACCCGTGTCATCCTCCCTCTACGACCTCACCAACGTGGACAC; CACCACGGACAACTCAGTGCTGGAAATCACTGTCTACAACACCAACATCGACAACCGGCATGAGATGCTG; ACCCTGGAGCCGCTGCACACGCTGCTGCATATGAAGTGGAAGAAGTTTGCCAAGCACATGTTCTTTCTGT; CCTTCTGCTTTTATTTCTTCTACAACATCACCCTGACCCTCGTCTCGTACTACCGCCCCCGGGAGGAGGA; GGCCATCCCGCACCCCTTGGCCCTGACGCACAAGATGGGGTGGCTGCAGCTCCTAGGGAGGATGTTTGTG; CTCATCTGGGCCATGTGCATCTCTGTGAAAGAGGGCATTGCCATCTTCCTGCTGAGACCCTCGGATCTGC; AGTCCATCCTCTCGGATGCCTGGTTCCACTTTGTCTTTTTTATCCAAGCTGTGCTTGTGATACTGTCTGT; CTTCTTGTACTTGTTTGCCTACAAAGAGTACCTCGCCTGCCTCGTGCTGGCCATGGCCCTGGGCTGGGCG; AACATGCTCTACTATACGCGGGGTTTCCAGTCCATGGGCATGTACAGCGTCATGATCCAGAAGGTCATTT; TGCATGATGTTCTGAAGTTCTTGTTTGTATATATCGTGTTTTTGCTTGGATTTGGAGTAGCCTTGGCCTC; GCTGATCGAGAAGTGTCCCAAAGACAACAAGGACTGCAGCTCCTACGGCAGCTTCAGCGACGCAGTGCTG; GAACTCTTCAAGCTCACCATAGGCCTGGGTGATCTGAACATCCAGCAGAACTCCAAGTATCCCATTCTCT; TTCTGTTCCTGCTCATCACCTATGTCATCCTCACCTTTGTTCTCCTCCTCAACATGCTCATTGCTCTGAT; GGGCGAGACTGTGGAGAACGTCTCCAAGGAGAGCGAACGCATCTGGCGCCTGCAGAGAGCCAGGACCATC; TTGGAGTTTGAGAAAATGTTACCAGAATGGCTGAGGAGCAGATTCCGGATGGGAGAGCTGTGCAAAGTGG; CCGAGGATGATTTCCGACTGTGTTTGCGGATCAATGAGGTGAAGTGGACTGAATGGAAGACGCACGTCTC; CTTCCTTAACGAAGACCCGGGGCCTGTAAGACGAACAGCAGATTTCAACAAAATCCAAGATTCTTCCAGG; AACAACAGCAAAACCACTCTCAATGCATTTGAAGAAGTCGAGGAATTCCCGGAAACCTCGGTG; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC211184 protein sequence; Red=Cloning site Green=Tags(s); ; MKAHPKEMVPLMGKRVAAPSGNPAVLPEKRPAEITPTKKSAHFFLEIEGFEPNPTVAKTSPPVFSKPMDS; NIRQCISGNCDDMDSPQSPQDDVTETPSNPNSPSAQLAKEEQRRKKGRLKKRIFAAVSEGCVEELVELLV; ELQELCRRRHDEDVPDFLMHKLTASDTGKTCLMKALLNINPNTKEIVRILLAFAEENNILGRFINAEYTE; EAYEGQTALNIAIERRQGDIAALLIAAGADVNAHAKGAFFNPKYQHEGFYFGETPLALAACTNQPEIVQL; LMEHEQTDITSRDSRGNNILHALVTVAEDFKTQNDFVKRMYDMILLRSGNWELETTRNNDGLTPLQLAAK; MGKAEILKYILSREIKEKRLRSLSRKFTDWAYGPVSSSLYDLTNVDTTTDNSVLEITVYNTNIDNRHEML; TLEPLHTLLHMKWKKFAKHMFFLSFCFYFFYNITLTLVSYYRPREEEAIPHPLALTHKMGWLQLLGRMFV; LIWAMCISVKEGIAIFLLRPSDLQSILSDAWFHFVFFIQAVLVILSVFLYLFAYKEYLACLVLAMALGWA; NMLYYTRGFQSMGMYSVMIQKVILHDVLKFLFVYIVFLLGFGVALASLIEKCPKDNKDCSSYGSFSDAVL; ELFKLTIGLGDLNIQQNSKYPILFLFLLITYVILTFVLLLNMLIALMGETVENVSKESERIWRLQRARTI; LEFEKMLPEWLRSRFRMGELCKVAEDDFRLCLRINEVKWTEWKTHVSFLNEDPGPVRRTADFNKIQDSSR; NNSKTTLNAFEEVEEFPETSV; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Chromatograms: Chromatograms ; Sequencher program is needed, download here
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_145068
• ORF Size: 2373 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_145068.4
• RefSeq Size: 6130 bp
• RefSeq ORF: 2373 bp
• Locus ID: 162514
• UniProt ID: Q8NET8
• Cytogenetics: 17p13.2
• Protein Families: Druggable Genome, Ion Channels: Transient receptor potential, Transmembrane
• MW: 90.6 kDa
• Summary: This gene product belongs to a family of nonselective cation channels that function in a variety of processes, including temperature sensation and vasoregulation. The thermosensitive members of this family are expressed in subsets of sensory neurons that terminate in the skin, and are activated at distinct physiological temperatures. This channel is activated at temperatures between 22 and 40 degrees C. This gene lies in close proximity to another family member gene on chromosome 17, and the two encoded proteins are thought to associate with each other to form heteromeric channels. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2012]