PTPRS (NM_130853) Human Tagged ORF Clone

SKU RC211163

PTPRS (Myc-DDK-tagged)-Human protein tyrosine phosphatase, receptor type, S (PTPRS), transcript variant 3

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: PTPRS
• Synonyms: PTPSIGMA; R-PTP-S; R-PTP-sigma
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC211163 ORF sequence; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGCGCCCACCTGGGGCCCTGGCATGGTGTCTGTGGTTGGTCCCATGGGCCTCCTTGTGGTCCTGCTCG; TTGGAGGCTGTGCAGCAGAAGAGCCCCCCAGGTTTATCAAAGAACCCAAGGACCAGATCGGCGTGTCGGG; GGGTGTGGCCTCTTTCGTGTGTCAGGCCACGGGTGACCCCAAGCCACGAGTGACCTGGAACAAGAAGGGC; AAGAAGGTCAACTCTCAGCGCTTTGAGACGATTGAGTTTGATGAGAGTGCAGGGGCAGTGCTGAGGATCC; AGCCGCTGAGGACACCGCGGGATGAAAACGTGTACGAGTGTGTGGCCCAGAACTCGGTTGGGGAGATCAC; AGTCCATGCCAAGCTTACTGTCCTCCGAGAGGACCAGCTGCCCTCTGGCTTCCCCAACATCGACATGGGC; CCACAGTTGAAGGTGGTGGAGCGGACACGGACAGCCACCATGCTCTGTGCAGCCAGCGGCAACCCTGACC; CTGAGATCACCTGGTTCAAGGACTTCCTGCCTGTGGATCCTAGTGCCAGCAATGGACGCATCAAACAGCT; GCGATCAGGAGCCCTGCAGATTGAAAGCAGTGAGGAAACCGACCAGGGCAAATATGAGTGTGTGGCCACC; AACAGCGCCGGCGTGCGCTACTCCTCACCTGCCAACCTCTACGTGCGAGTCCGCCGCGTGGCCCCGCGCT; TCTCCATCCTGCCCATGAGCCACGAGATCATGCCAGGGGGCAACGTGAACATCACCTGCGTGGCCGTGGG; CTCGCCCATGCCATACGTGAAGTGGATGCAGGGGGCCGAGGACCTGACCCCCGAGGATGACATGCCCGTG; GGTCGGAACGTGCTGGAACTCACAGATGTCAAGGACTCGGCCAACTACACCTGCGTGGCCATGTCCAGCC; TGGGCGTCATTGAGGCGGTTGCTCAGATCACGGTGAAATCTCTCCCCAAAGCTCCCGGGACTCCCATGGT; GACTGAGAACACAGCCACCAGCATCACCATCACGTGGGACTCGGGCAACCCAGATCCTGTGTCCTATTAC; GTCATCGAATATAAATCCAAGAGCCAAGACGGGCCGTATCAGATTAAAGAGGACATCACCACCACACGTT; ACAGCATCGGCGGCCTGAGCCCCAACTCGGAGTACGAGATCTGGGTGTCGGCCGTCAACTCCATCGGCCA; GGGGCCCCCCAGCGAGTCCGTGGTCACCCGCACAGGCGAGCAGGCCCCGGCCAGCGCGCCGCGGAACGTG; CAAGCCCGGATGCTCAGCGCGACCACCATGATTGTGCAGTGGGAGGAGCCGGTGGAGCCCAACGGCCTGA; TCCGCGGCTACCGCGTCTACTACACCATGGAACCGGAGCACCCCGTGGGCAACTGGCAGAAGCACAACGT; GGACGACAGCCTGCTGACCACCGTGGGCAGCCTGCTGGAGGACGAGACCTACACCGTGCGGGTGCTCGCC; TTCACCTCCGTCGGCGACGGGCCCCTCTCGGACCCCATCCAGGTCAAGACGCAGCAGGGAGTGCCGGGCC; AGCCCATGAACCTGCGGGCCGAGGCCAGGTCGGAGACCAGCATCACGCTGTCCTGGAGCCCCCCGCGGCA; GGAGAGTATCATCAAGTACGAGCTCCTCTTCCGGGAAGGCGACCATGGCCGGGAGGTGGGAAGGACCTTC; GACCCGACGACTTCCTACGTGGTGGAGGACCTGAAGCCCAACACGGAGTACGCCTTCCGCCTGGCGGCCC; GCTCGCCGCAGGGCCTGGGCGCCTTCACCCCCGTGGTGCGGCAGCGCACGCTGCAGTCCATCTCGCCCAA; GAACTTCAAGGTGAAAATGATCATGAAGACATCAGTTCTGCTCAGCTGGGAGTTCCCTGACAACTACAAC; TCACCCACACCCTACAAGATCCAGTACAATGGGCTCACACTGGATGTGGATGGCCGTACCACCAAGAAGC; TCATCACGCACCTCAAGCCCCACACCTTCTACAACTTTGTGCTGACCAATCGCGGCAGCAGCCTGGGCGG; CCTCCAGCAGACGGTCACCGCCTGGACTGCCTTCAACCTGCTCAACGGCAAGCCCAGCGTCGCCCCCAAG; CCTGATGCTGACGGCTTCATCATGGTGTATCTTCCTGACGGCCAGAGCCCCGTGCCTGTCCAGAGCTATT; TCATTGTGATGGTGCCACTGCGCAAGTCTCGTGGAGGCCAATTCCTGACCCCGCTGGGTAGCCCAGAGGA; CATGGATCTGGAAGAGCTCATCCAGGACATCTCACGGCTACAGAGGCGCAGCCTGCGGCACTCGCGTCAG; CTGGAGGTGCCCCGGCCCTATATTGCAGCTCGCTTCTCTGTGCTGCCACCCACGTTCCATCCCGGCGACC; AGAAGCAGTATGGCGGCTTCGATAACCGGGGCCTGGAGCCCGGCCACCGCTATGTCCTCTTCGTGCTTGC; CGTGCTTCAGAAGAGCGAGCCTACCTTTGCAGCCAGTCCCTTCTCAGACCCCTTCCAGCTGGATAACCCG; GACCCCCAGCCCATCGTGGATGGCGAGGAGGGGCTTATCTGGGTGATCGGGCCTGTGCTGGCCGTGGTCT; TCATAATCTGCATTGTCATTGCTATCCTGCTCTACAAGAACAAACCCGACAGTAAACGCAAGGACTCAGA; ACCCCGCACCAAATGCCTCCTGAACAATGCCGACCTCGCCCCTCACCACCCCAAGGACCCTGTGGAAATG; AGACGCATTAACTTCCAGACTCCAGGCATGCTTAGCCACCCGCCAATTCCCATCGCAGACATGGCGGAGC; ACACGGAGCGGCTCAAGGCCAACGACAGCCTCAAGCTCTCCCAGGAGTATGAGTCCATCGACCCTGGACA; GCAGTTCACATGGGAACATTCCAACCTGGAAGTGAACAAGCCGAAGAACCGCTATGCCAACGTCATCGCC; TATGACCACTCCCGTGTCATCCTCCAGCCCATTGAAGGCATCATGGGCAGTGATTACATCAATGCCAACT; ACGTGGACGGCTACCGGCGTCAGAACGCGTACATTGCCACGCAGGGGCCGCTGCCTGAGACCTTTGGGGA; CTTCTGGCGTATGGTGTGGGAGCAGCGGTCGGCGACCATCGTCATGATGACGCGGCTGGAGGAGAAGTCA; CGGATCAAGTGTGATCAGTATTGGCCCAACAGAGGCACGGAGACCTACGGCTTCATCCAGGTCACGTTGC; TAGATACCATCGAGCTGGCCACATTCTGCGTCAGGACATTCTCTCTGCACAAGAATGGCTCCAGTGAGAA; ACGCGAGGTCCGCCAGTTCCAGTTTACGGCGTGGCCGGACCATGGCGTGCCCGAATACCCAACGCCCTTC; CTGGCTTTCCTGCGGAGAGTCAAGACCTGCAACCCGCCAGATGCCGGCCCCATCGTGGTTCACTGCAGTG; CCGGTGTGGGCCGCACAGGCTGCTTTATCGTCATCGACGCCATGCTTGAGCGGATCAAGCCAGAGAAGAC; AGTCGATGTCTATGGCCACGTGACGCTCATGAGGTCCCAGCGCAACTACATGGTGCAGACGGAGGACCAG; TACAGCTTCATCCACGAGGCCCTGCTGGAGGCCGTGGGCTGTGGCAACACAGAAGTGCCCGCACGCAGCC; TCTATGCCTACATCCAGAAGCTGGCCCAGGTGGAGCCTGGCGAACACGTCACTGGCATGGAACTCGAGTT; CAAGCGGCTGGCTAACTCCAAGGCCCACACGTCACGCTTCATCAGTGCCAATCTGCCTTGTAACAAGTTC; AAGAACCGCCTGGTGAACATCATGCCCTATGAGAGCACACGGGTCTGTCTGCAACCCATCCGGGGTGTGG; AGGGCTCTGACTACATCAACGCCAGCTTCATTGATGGCTACAGGCAGCAGAAGGCCTACATCGCGACACA; GGGGCCGCTGGCGGAGACCACGGAAGACTTCTGGCGCATGCTGTGGGAGAACAATTCGACGATCGTGGTG; ATGCTGACCAAGCTGCGGGAGATGGGCCGGGAGAAGTGTCACCAGTACTGGCCGGCCGAGCGCTCTGCCC; GCTACCAGTACTTTGTGGTAGATCCGATGGCAGAATACAACATGCCTCAGTATATCCTGCGAGAGTTCAA; GGTCACAGATGCCCGGGATGGCCAGTCCCGGACTGTCCGGCAGTTCCAGTTCACAGACTGGCCGGAACAG; GGTGTGCCAAAGTCGGGGGAGGGCTTCATCGACTTCATTGGCCAAGTGCATAAGACTAAGGAGCAGTTTG; GCCAGGACGGCCCCATCTCTGTCCACTGCAGTGCCGGCGTGGGCAGGACGGGCGTCTTCATCACGCTTAG; CATCGTGCTGGAGCGGATGCGGTATGAAGGCGTGGTGGACATCTTTCAGACGGTGAAGATGCTACGAACC; CAGCGGCCGGCCATGGTGCAGACAGAGGATGAGTACCAGTTCTGTTACCAGGCGGCACTGGAGTACCTCG; GAAGCTTTGACCACTATGCAACC; ; AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC; TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC211163 protein sequence; Red=Cloning site Green=Tags(s); ; MAPTWGPGMVSVVGPMGLLVVLLVGGCAAEEPPRFIKEPKDQIGVSGGVASFVCQATGDPKPRVTWNKKG; KKVNSQRFETIEFDESAGAVLRIQPLRTPRDENVYECVAQNSVGEITVHAKLTVLREDQLPSGFPNIDMG; PQLKVVERTRTATMLCAASGNPDPEITWFKDFLPVDPSASNGRIKQLRSGALQIESSEETDQGKYECVAT; NSAGVRYSSPANLYVRVRRVAPRFSILPMSHEIMPGGNVNITCVAVGSPMPYVKWMQGAEDLTPEDDMPV; GRNVLELTDVKDSANYTCVAMSSLGVIEAVAQITVKSLPKAPGTPMVTENTATSITITWDSGNPDPVSYY; VIEYKSKSQDGPYQIKEDITTTRYSIGGLSPNSEYEIWVSAVNSIGQGPPSESVVTRTGEQAPASAPRNV; QARMLSATTMIVQWEEPVEPNGLIRGYRVYYTMEPEHPVGNWQKHNVDDSLLTTVGSLLEDETYTVRVLA; FTSVGDGPLSDPIQVKTQQGVPGQPMNLRAEARSETSITLSWSPPRQESIIKYELLFREGDHGREVGRTF; DPTTSYVVEDLKPNTEYAFRLAARSPQGLGAFTPVVRQRTLQSISPKNFKVKMIMKTSVLLSWEFPDNYN; SPTPYKIQYNGLTLDVDGRTTKKLITHLKPHTFYNFVLTNRGSSLGGLQQTVTAWTAFNLLNGKPSVAPK; PDADGFIMVYLPDGQSPVPVQSYFIVMVPLRKSRGGQFLTPLGSPEDMDLEELIQDISRLQRRSLRHSRQ; LEVPRPYIAARFSVLPPTFHPGDQKQYGGFDNRGLEPGHRYVLFVLAVLQKSEPTFAASPFSDPFQLDNP; DPQPIVDGEEGLIWVIGPVLAVVFIICIVIAILLYKNKPDSKRKDSEPRTKCLLNNADLAPHHPKDPVEM; RRINFQTPGMLSHPPIPIADMAEHTERLKANDSLKLSQEYESIDPGQQFTWEHSNLEVNKPKNRYANVIA; YDHSRVILQPIEGIMGSDYINANYVDGYRRQNAYIATQGPLPETFGDFWRMVWEQRSATIVMMTRLEEKS; RIKCDQYWPNRGTETYGFIQVTLLDTIELATFCVRTFSLHKNGSSEKREVRQFQFTAWPDHGVPEYPTPF; LAFLRRVKTCNPPDAGPIVVHCSAGVGRTGCFIVIDAMLERIKPEKTVDVYGHVTLMRSQRNYMVQTEDQ; YSFIHEALLEAVGCGNTEVPARSLYAYIQKLAQVEPGEHVTGMELEFKRLANSKAHTSRFISANLPCNKF; KNRLVNIMPYESTRVCLQPIRGVEGSDYINASFIDGYRQQKAYIATQGPLAETTEDFWRMLWENNSTIVV; MLTKLREMGREKCHQYWPAERSARYQYFVVDPMAEYNMPQYILREFKVTDARDGQSRTVRQFQFTDWPEQ; GVPKSGEGFIDFIGQVHKTKEQFGQDGPISVHCSAGVGRTGVFITLSIVLERMRYEGVVDIFQTVKMLRT; QRPAMVQTEDEYQFCYQAALEYLGSFDHYAT; ; SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Chromatograms: Chromatograms ; Sequencher program is needed, download here
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_130853
• ORF Size: 4503 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.; ; The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_130853.3
• RefSeq Size: 6006 bp
• RefSeq ORF: 4506 bp
• Locus ID: 5802
• UniProt ID: Q13332
• Cytogenetics: 19p13.3
• Protein Families: Druggable Genome, Phosphatase, Transmembrane
• MW: 168.4 kDa
• Summary: The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains an extracellular region, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus represents a receptor-type PTP. The extracellular region of this protein is composed of multiple Ig-like and fibronectin type III-like domains. Studies of the similar gene in mice suggested that this PTP may be involved in cell-cell interaction, primary axonogenesis, and axon guidance during embryogenesis. This PTP has been also implicated in the molecular control of adult nerve repair. Four alternatively spliced transcript variants, which encode distinct proteins, have been reported. [provided by RefSeq, Jul 2008]