CDC14A (NM_033313) Human Tagged ORF Clone

SKU RC210878

CDC14A (Myc-DDK-tagged)-Human CDC14 cell division cycle 14 homolog A (S. cerevisiae) (CDC14A), transcript variant 3

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: CDC14A
• Synonyms: cdc14; DFNB32; DFNB35; DFNB105; hCDC14
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC210878 representing NM_033313; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGCAGCGGAGTCAGGGGAACTAATCGGGGCTTGTGAGTTCATGAAAGATCGGTTATATTTTGCTACTT; TAAGGAATAGACCAAAAAGCACAGTAAATACCCACTATTTCTCCATCGATGAGGAGCTGGTCTATGAAAA; TTTCTATGCAGATTTTGGACCGCTGAACTTGGCAATGGTGTACAGATATTGCTGCAAACTAAACAAGAAA; CTAAAATCATACAGTTTGTCAAGAAAGAAAATAGTGCACTACACCTGTTTTGACCAACGGAAAAGAGCAA; ATGCAGCATTTTTGATAGGTGCCTATGCAGTAATCTATTTAAAGAAGACACCAGAAGAAGCCTACAGAGC; ACTCCTGTCTGGCTCAAACCCCCCCTATCTTCCATTCAGGGATGCTTCCTTTGGAAATTGCACTTACAAT; CTCACCATTCTCGACTGTTTGCAGGGAATCAGAAAGGGATTACAACATGGATTTTTTGACTTTGAGACAT; TTGATGTGGATGAATATGAACATTATGAGCGAGTTGAAAATGGTGACTTCAACTGGATTGTTCCAGGAAA; ATTTTTAGCATTTAGTGGACCACATCCTAAAAGCAAAATTGAGAATGGTTATCCTCTTCACGCCCCTGAA; GCCTACTTTCCTTATTTCAAAAAGCATAATGTGACTGCAGTTGTGAGGCTAAACAAAAAGATTTATGAGG; CAAAGCGCTTCACAGACGCTGGCTTCGAGCACTATGACCTCTTCTTCATAGATGGCAGCACACCCAGTGA; CAACATCGTGCGAAGGTTCCTGAACATCTGTGAGAACACCGAAGGGGCCATCGCCGTTCACTGCAAAGCT; GGTCTTGGAAGAACAGGGACATTGATAGCCTGTTATGTAATGAAACACTACAGGTTTACACATGCTGAAA; TAATTGCTTGGATTAGAATATGCCGGCCAGGCTCTATTATAGGACCCCAGCAGCACTTCCTGGAAGAAAA; ACAAGCATCGTTGTGGGTCCAAGGAGACATTTTCCGATCCAAACTGAAAAATCGACCATCCAGTGAAGGA; AGTATTAATAAAATTCTTTCTGGCCTAGATGATATGTCTATTGGTGGAAATCTTTCAAAAACACAAAACA; TGGAACGATTTGGAGAGGTAAGTTTTCCC; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC210878 representing NM_033313; Red=Cloning site Green=Tags(s); ; MAAESGELIGACEFMKDRLYFATLRNRPKSTVNTHYFSIDEELVYENFYADFGPLNLAMVYRYCCKLNKK; LKSYSLSRKKIVHYTCFDQRKRANAAFLIGAYAVIYLKKTPEEAYRALLSGSNPPYLPFRDASFGNCTYN; LTILDCLQGIRKGLQHGFFDFETFDVDEYEHYERVENGDFNWIVPGKFLAFSGPHPKSKIENGYPLHAPE; AYFPYFKKHNVTAVVRLNKKIYEAKRFTDAGFEHYDLFFIDGSTPSDNIVRRFLNICENTEGAIAVHCKA; GLGRTGTLIACYVMKHYRFTHAEIIAWIRICRPGSIIGPQQHFLEEKQASLWVQGDIFRSKLKNRPSSEG; SINKILSGLDDMSIGGNLSKTQNMERFGEVSFP; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_033313
• ORF Size: 1149 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_033313.2, NP_201570.1
• RefSeq Size: 1900 bp
• RefSeq ORF: 1152 bp
• Locus ID: 8556
• UniProt ID: Q9UNH5
• Cytogenetics: 1p21.2
• Protein Families: Druggable Genome, Phosphatase
• Protein Pathways: Cell cycle
• MW: 43.7 kDa
• Summary: The protein encoded by this gene is a member of the dual specificity protein tyrosine phosphatase family. It is highly similar to Saccharomyces cerevisiae Cdc14, a protein tyrosine phosphatase involved in the exit of cell mitosis and initiation of DNA replication, suggesting a role in cell cycle control. This protein has been shown to interact with, and dephosphorylate tumor suppressor protein p53, and is thought to regulate the function of p53. Alternative splicing of this gene results in several transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2008]