ZP4 (NM_021186) Human Tagged ORF Clone

SKU RC210177

ZP4 (Myc-DDK-tagged)-Human zona pellucida glycoprotein 4 (ZP4)

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: ZP4
• Synonyms: ZBP; Zp-4; ZP1; ZPB
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC210177 representing NM_021186; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGTGGCTGCTGCGGTGCGTTTTGCTGTGTGTTTCATTATCTCTTGCTGTGAGTGGCCAGCATAAGCCTG; AGGCACCAGATTATTCCAGTGTGCTCCACTGTGGGCCGTGGAGCTTCCAGTTTGCTGTAAACCTCAACCA; GGAGGCAACGTCTCCTCCTGTACTAATAGCTTGGGACAACCAAGGGCTGCTGCACGAGCTGCAGAATGAC; TCCGACTGTGGCACCTGGATAAGAAAAGGTCCAGGCAGCTCCGTGGTGTTGGAGGCAACCTATAGCAGCT; GCTATGTCACTGAGTGGGACTCCCACTACATCATGCCAGTTGGAGTTGAAGGAGCAGGCGCGGCTGAACA; CAAGGTGGTTACAGAGAGGAAGCTGCTCAAGTGTCCTATGGATCTTCTAGCCCGAGATGCTCCAGATACT; GACTGGTGTGACTCCATCCCAGCACGGGACAGACTGCCATGTGCACCTTCACCCATCTCTCGAGGAGACT; GTGAAGGGCTAGGCTGTTGTTATAGCTCTGAAGAGGTGAATTCCTGCTACTATGGAAACACTGTGACCTT; GCATTGTACCCGAGAGGGCCATTTCTCTATTGCTGTGTCTCGGAACGTGACCTCGCCACCACTGCTCTTG; GATTCTGTGCGCTTGGCCCTTAGGAATGACAGTGCGTGTAACCCTGTGATGGCAACACAAGCTTTTGTTC; TGTTCCAGTTTCCATTTACTTCCTGTGGCACCACAAGACAGATCACTGGAGACCGAGCAGTATATGAAAA; TGAACTGGTGGCAACTAGGGATGTGAAAAATGGGAGCCGTGGCTCTGTCACTCGTGACAGCATCTTCAGG; CTCCATGTCAGCTGCAGCTACTCAGTAAGTAGCAACTCTCTCCCAATCAATGTCCAGGTTTTCACTCTCC; CACCACCCTTTCCTGAGACCCAGCCTGGACCCCTCACTCTGGAACTTCAGATTGCCAAAGATAAAAACTA; TGGCTCTTACTACGGTGTTGGTGACTACCCAGTGGTGAAGTTGCTTCGGGATCCCATTTACGTGGAGGTC; TCCATCCTTCACAGAACAGACCCCTACCTGGGGCTGCTCCTACAACAGTGTTGGGCAACACCCAGCACTG; ACCCCCTGAGTCAGCCACAGTGGCCCATCCTGGTAAAGGGCTGCCCCTACATTGGAGACAACTATCAGAC; CCAGCTGATCCCTGTCCAGAAAGCCTTGGATCTTCCATTTCCCTCTCACCACCAGCGCTTCAGCATCTTC; ACCTTCAGCTTTGTGAACCCTACAGTGGAGAAACAGGCCCTCAGGGGACCGGTGCATCTGCACTGCAGCG; TGTCAGTCTGCCAGCCTGCTGAGACACCATCCTGTGTGGTGACCTGTCCTGATCTCAGTCGAAGAAGAAA; TTTTGACAACAGTTCTCAGAACACTACTGCTAGTGTTTCTAGCAAAGGCCCCATGATTCTACTCCAAGCC; ACTAAGGACCCTCCAGAAAAGCTCCGTGTTCCTGTAGACTCGAAAGTTCTGTGGGTGGCAGGCCTTTCTG; GGACCTTAATCCTTGGAGCCTTGTTAGTATCCTACTTGGCTGTCAAGAAACAGAAGAGTTGCCCAGACCA; AATGTGTCAA; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC210177 representing NM_021186; Red=Cloning site Green=Tags(s); ; MWLLRCVLLCVSLSLAVSGQHKPEAPDYSSVLHCGPWSFQFAVNLNQEATSPPVLIAWDNQGLLHELQND; SDCGTWIRKGPGSSVVLEATYSSCYVTEWDSHYIMPVGVEGAGAAEHKVVTERKLLKCPMDLLARDAPDT; DWCDSIPARDRLPCAPSPISRGDCEGLGCCYSSEEVNSCYYGNTVTLHCTREGHFSIAVSRNVTSPPLLL; DSVRLALRNDSACNPVMATQAFVLFQFPFTSCGTTRQITGDRAVYENELVATRDVKNGSRGSVTRDSIFR; LHVSCSYSVSSNSLPINVQVFTLPPPFPETQPGPLTLELQIAKDKNYGSYYGVGDYPVVKLLRDPIYVEV; SILHRTDPYLGLLLQQCWATPSTDPLSQPQWPILVKGCPYIGDNYQTQLIPVQKALDLPFPSHHQRFSIF; TFSFVNPTVEKQALRGPVHLHCSVSVCQPAETPSCVVTCPDLSRRRNFDNSSQNTTASVSSKGPMILLQA; TKDPPEKLRVPVDSKVLWVAGLSGTLILGALLVSYLAVKKQKSCPDQMCQ; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Chromatograms: Chromatograms ; Sequencher program is needed, download here
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_021186
• ORF Size: 1620 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_021186.4
• RefSeq Size: 2474 bp
• RefSeq ORF: 1623 bp
• Locus ID: 57829
• UniProt ID: Q12836
• Cytogenetics: 1q43
• Protein Families: Secreted Protein, Transmembrane
• MW: 59.4 kDa
• Summary: The zona pellucida is an extracellular matrix that surrounds the oocyte and early embryo. It is composed primarily of three or four glycoproteins with various functions during fertilization and preimplantation development. The nascent protein contains a N-terminal signal peptide sequence, a conserved ZP domain, a consensus furin cleavage site, and a C-terminal transmembrane domain. It is hypothesized that furin cleavage results in release of the mature protein from the plasma membrane for subsequent incorporation into the zona pellucida matrix. However, the requirement for furin cleavage in this process remains controversial based on mouse studies. Previously, this gene has been referred to as ZP1 or ZPB and thought to have similar functions as mouse Zp1. However, a human gene with higher similarity and chromosomal synteny to mouse Zp1 has been assigned the symbol ZP1 and this gene has been assigned the symbol ZP4. [provided by RefSeq, Jul 2008]